Establishment and evaluation of psychological stress asthmatic rat model*
2016, 39 (12):
Objective To induce the psychological stress-asthmatic rat model by ovalbumin (OVA)mixture sensitization with restraint, which simulated the pathogenic factors of bronchial asthma with liver-qi stagnation pattern, as well as to evaluate the model using indicators of the macro characterization, behaviors, pulmonary function, peripheral blood inflammatory cells, bronchial tissue pathomorphology. Methods A total 32 male rats were randomly divided into four groups—control group, stress group, asthmatic group, and stress-asthmatic group. Rats of stress group and stress-asthmatic group were immobilized in restrained box once daily at different time, ten minutes more than the day before with the first duration of 2 hours. Rats of stress-asthmatic group and asthmatic group were sensitized by intraperitoneal injection 1 mL mixture of OVA with aluminium hydroxide on the first day, and after 13 days, they were stimulated by inhalation of 1 % OVA for 20 min for consecutive 14 days. The rats of control group were sensitized and stimulated by saline, without immobilizing . 28 days later, the macro characterization, behaviors, peripheral blood eosinophils, pulmonary function, bronchial tissue pathomorphology were observed. Results Stress asthmatic rats showed low mood, loss of interest, dull hair,loose stools, reducing food intake,slowly weight gain, and scratched noses and abdominal skin, coughed, sneezed and wheezed in the throat when stimulated. Compared with control group, the points of vertical movement decreased significantly(P＜0.05 or P＜0.01). Preference for sucrose, the times of active moves in tail suspension test (TST) of stress-asthmatic rats obviously decreased(P＜0.01), and the duration of immobility increased significantly(P＜0.01). Pulmonary function indicators, including peak expiratory flow(PEF), forced expiratory flow (FEF 25%-75% ), forced expiratory volume in 0.3 s [FEV(0.3]/forced expiration volume capacity (FVC) significantly declined(P＜0.05,P＜0.01,P＜0.01), while mean inspiratory airway resistance (Ri) and mean expiratory airway resistance (Re) increased remarkably (P＜0.05,P＜0.01).Peripheral blood eosinophil cells increased(P＜0.01), and infiltration of inflammatory cell in lung tissue was obvious. Compared with the stress group or asthmatic group, the signs and indicators of stress-asthmatic group were all much worse. Conlusion The psychological stress-asthmatic rat model, induced by VOA mixture with restraint, was proved to be successful. It is in accordance with clinical characteristics of bronchial asthma with liver-qi stagnation pattern, which can be used to study asthma caused by emotion .
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