The pou1f1 target genes expression characteristic in pituitary gland of H22 tumor-bearing mice with different TCM patterns*
2017, 40 (2):
Objective To study the expression of pituitary key functional genes and their pituitary-specific transcription factor 1 (Pou1fl) gene in H22 tumor-bearing mice with different TCM patterns. Methods 190 Kunming mice were injected subcutaneously into the armpit with H22 hepatoma carcinoma cells, and 60 more healthy mice were assigned into the normal group. The mice with pattern of exuberance of pathogenic toxin, asthenia of qi, asthenia of yang and qi, or asthenia of qi, yin and yang were identified by using the method of mice standardization of four diagnosis information and syndrome differentiation developed by our team. Then RNA was extracted from pituitary gland, hypothalamus and testis and was detected using GeneChip Mouse Exon 1.0 ST Array. And the genetic data of pituitary gland of different patterns were analyzed. Results Compared with normal group, the expression of pituitary key functional genes including Pomc, Gh and Prl were decreased by about 10% in mice with pattern of exuberance of pathogenic toxin or asthenia of qi, which was the pattern of early stage. At the middle or late stage, TCM patters were the pattern of asthenia of yang and qi, or asthenia of qi, yin and yang; Pomc, Gh, Prl and Tshb were upregulated by more than 30%. Pou1fl expressed at the highest level in pituitary tissue with the characteristic of tissue specific expression. Pou1f1 and Neurod1 were only upregulated in pattern of exuberance of pathogenic toxin, Tbx19 and Egr1 were upregulated in all patterns, which was just opposite to that of Nr4a1, Nr4a2, Pitx1, Gata2, Nr4a3, and Foxl2. Poulfl target genes including Crem, Tcf4, Rnf41, Lrpprc, Mrps18b, Eif3a, Lars, Arf4, Cep57, Vcpip1, Ptpn6, Myd88, F3, Lrrfip3 and Dixdc1 all expressed in the similar pattern with that of Poulfl, upregulated in pattern of exuberance of pathogenic toxin. Conclusion The abnormal expression of pituitary key functional genes was proved in the tumor-bearing mice with different TCM patterns. Pituitary-specific transcription factor 1 with its target genes expressed in the similar way in the pattern of exuberance of pathogenic toxin.
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