Protective effects of Jiedu Tongluo Fang on rats with diabetic cardiomyopathy*
2017, 40 (8):
Objective To explore the possible mechanism of Jiedu Tongluo Fang(Toxin-removing collateral-unblocking Formula, JDTLF) in the treatment of diabetic cardiomyopathy by observing its effects on the expression of peroxisome proliferator activated receptor gamma(PPARγ)and cardiac function in rats with diabetic cardiomyopathy. Methods 100 Wistar rats were randomly divided into normal control group (n=8) and diabetic model group (n=92). Diabetic model was built by using intraperitoneal injection of streptozotocin (STZ, 55 mg/kg). Diabetic cardiomyopathy(DCM) model was built by feeding those rats for another 12 consecutive weeks.DCM model rats were randomly divided into JDTLF group,Qingre JieduYao(heat-clearing toxin-removing medicinal, QRJDY)group, pioglitazone group, and DCM model group.Except the DCM model group and the normal control group, the other 3 groups were orally gavaged with 10.4 g/(kg·d) JDTLF,2.7 g/(kg·d) QRJDY,and 2.7 mg/(kg·d) pioglitazone respectively. DCM group and NC group received distilled water for 6 weeks. At the end of the experiment, random blood glucose (RBG), blood lipid(TC, TG, LDL-C, and HDL-C), high sensitive C reactive protein (hs-CRP), cardiac function(LVSP,LVEDP,+dp/dtmax,-dp/dtmax and t-dp/dt) and morphological changes were evaluated. Expression of PPARγ in myocardium was measured with immunohistochemistry. Results Compared with the NC group, body weight in DCM group decreased significantly (P＜0.01); Random blood glucose,hs-CRP,TC, TG and LDL-C increased significantly(P＜0.01,P＜0.05); HDL-C decreased significantly (P＜0.05),and cardiac function decreased significantly; LVSP, +dp/dtmax,-dp/dtmax decreased significantly; LVEDP and t-dp/dt increased significantly (P＜0.05). There was a significant decrease in the expression of myocardial PPARγ; Compared with DCM model group, random blood glucose in JDTLF group and QRJDY group decreased significantly;Hs-CRP,TC, TG and LDL-C decreased significantly (P＜0.01,P＜0.05), HDL-C increased significantly(P＜0.05); Cardiac function improved in JDTLF group and QRJDY group; LVSP, +dp/dtmax and -dp/dtmax increased significantly (P＜0.05); LVEDP and t-dp/dt decreased significantly (P＜0.05); Expression of myocardial PPARγ presented an increasing tendency (P＜0.01). Conclusion Jeidu Tongluo Fang and Qingre Jiedu Yao can significantly improve the glucose and lipid metabolism, heart function and heart tissue structure, and have protective effect on diabetic cardiomyopathy.Its mechanism may be related to the up-regulation of PPARγ.
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