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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    10 October 2017, Volume 40 Issue 10 Previous Issue    Next Issue
    Scientific nature of traditional Chinese medicine in post-modern era: a commentary
    WANG Yongyan
    2017, 40 (10):  797-802.  doi: 10.3969/j.issn.1006-2157.2017.10.001
    Abstract ( 469 )   PDF (1309KB) ( 392 )   Save
    In post-modern era, it is essential to study the scientific nature of traditional Chinese medicine (TCM) in an ever-changing context. This paper analyzes the scientific and humanistic nature of TCM together with its unique image thinking ideology from the integration of science and humanities and interaction of image thinking and logical thinking. TCM theory is not based on mere materialism but on image thinking, which is of nonlinear uncertainty. It gives emphasis to the integration of entity and relation and change of energy and information. Research into the scientific and methodological questions about TCM in a complex systemic science should be aimed. The author holds that people-oriented medical value would guide the integration of science and humanity, holism and reductionism, image and logical thinking, systematic and descriptive studies, and evidence-based and narrative medicine, leading to the integration, harmonization and advancement of Western and Eastern medicine and generation of a new unified medicine.
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    On approaches to Western disease treated with Chinese medicine*
    HOU Yajing, CHEN Jiaxu, WANG Fangfang, LI Xiaojuan
    2017, 40 (10):  803-807.  doi: 10.3969/j.issn.1006-2157.2017.10.002
    Abstract ( 391 )   PDF (1230KB) ( 246 )   Save
    Approaches to Western disease treated with Chinese medicine include not only the thinking modality of TCM in the treatment of Western disease,but also relevant chinical and laboratory research on its efficacy and mechanism.The complexity and diversity of TCM treatment based on pattern identification together with non-standardized disease names of TCM affect how the approaches are formed. There are differences between these approaches and traditional treatment approaches in terms of method, objective, and use of formulas and herbs. Disease differentiation is primarily used with specific formula developed and modified for specific disease. The system of approaches still awaits further improvement and optimization so as to promote modernization of traditional Chinese medicine.
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    On the holistic view of the meridian sinew theory*
    LIN Xingxing,DONG Baoqiang
    2017, 40 (10):  808-812.  doi: 10.3969/j.issn.1006-2157.2017.10.003
    Abstract ( 508 )   PDF (1236KB) ( 1114 )   Save
    Current studies on the essence and theory of the meridian sinews are mainly based on anatomical approaches and lack the holistic view of traditional Chinese medicine (TCM). This paper explores the holistic relationship between the external nature, internal organs, and internal sinews; the inherent relationship between the sinews and qi and blood in movement, function and system; and the balanced relationship between up and down, left and right, front and back, angle and rectangle based on functional line. The authors hold that establishing the theoretical system of meridian sinews should be and must be placed in a multi-dimensional interconnected network so as to break the shackles of current tendon studies.
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    Combined formulas in traditional Chinese medicine and Kampo medicine: origin and comparison of clinical thinking*
    WANG Jin
    2017, 40 (10):  813-816.  doi: 10.3969/j.issn.1006-2157.2017.10.004
    Abstract ( 387 )   PDF (1167KB) ( 260 )   Save
    This paper compares the difference of combined formulas between traditional Chinese and Kampo medicine by contrasting“qi,blood,fluid and humor”theory of TCM and “qi,blood and water” theory of kampo medicine. In traditional Chinese medicine (TCM), combined formulas target at diseases of complicated pathogenesis with modified ingredients and dosage, which is featured by “multiple compound formula”. Whereas in Kampo medicine of Japan, “correspondence between formula and pattern” is emphasized for typical primary and secondary pattern. The combined formulas are used at the presence of multiple patterns with strictly standardized dosage and proportion. This emphasis on “correspondence between formula and pattern” tends to oversimplify the theory of Traditional Chinese Medicine and compromise its approach of treatment based on pattern identification. Combined formulas in TCM with multiple forms and layers are based on analysis of pathogenesis.
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    How traditional Chinese medicine views the body and nurtures life with physical exercises*
    LI Youqiang
    2017, 40 (10):  817-824.  doi: 10.3969/j.issn.1006-2157.2017.10.005
    Abstract ( 485 )   PDF (1269KB) ( 722 )   Save
    Compared with the body views in traditional Confucius and Daoism, traditional Chinese medicine (TCM) has broken through the traditional concept of “body and mind” and “mind is greater than the body”, putting the biological body in the ontological position. TCM views the body from the following three aspects: anatomical body focusing on physiological structure; qi-movement body focusing on movement; and emotional body focusing on emotional status. The anatomical body view corresponds to “neither internal nor external cause” and is against risky physical activities. It advocates sport safety to avoid any potential risk of injury. The qi-movement body view corresponds to “external cause” and advocates physical activities in line with the solar terms rather than otherwise. Emotional body view corresponds to “internal cause” and advocates common mild physical activities. It is against emerging stimulant sports. Therefore Daoyin is considered as the optimal form of exercise for nurturing life. Although those intense stimulant sports are not advocated in TCM body view, there is still room for these types of physical activities to exist, as the logic of TCM body view is to harmonize and regulate all physical activities.
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    Research on supplementary prescription of Qianjin Yaofang of Song Dynasty edition*
    LI Nan, ZENG Feng
    2017, 40 (10):  825-827.  doi: 10.3969/j.issn.1006-2157.2017.10.006
    Abstract ( 334 )   PDF (1224KB) ( 268 )   Save
    By comparing with Qianjin Yaofang of Song Dynasty edition and Xindiao Sunzhenren Qianjin Fang, the paper found that a large number of prescription were supplemented with Qianjin Yaofang of Song Dynasty edition. The supplementary prescription were selected according to the principle of understanding of medical knowledge and improvement of academic system, comparing with Qianjin Yifang, Waitai Miyao, Jingui Yaolue of Song Dynasty edition. The supplementary work pushed development of medical academic system, but destroyed original meaning of Qianjin Yaofang to a certain degree. Therefore, scholar value of Qianjin Yaofang of Song Dynasty edition should evaluated rationally based on confirmed contribution of Song scholars at the same time.
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    Textual research to identify the original plants for Kui and Dongkuizi*
    ZHOU Guanwu, QI Zhensheng, TIAN Jianfu, DI Guiying, WANG Tao, WU Chengliang, YANG Na
    2017, 40 (10):  828-832.  doi: 10.3969/j.issn.1006-2157.2017.10.007
    Abstract ( 375 )   PDF (1249KB) ( 622 )   Save
    This study aims to identify the original plants for Kui and Dongkuizi by reading and analyzing relevant historical literature. Misidentifications, misjudgments and false corrections by Japanese and Chinese experts as well as by modern and ancient scholars have been refuted and rectified based on comparing plant morphological characteristics, nomenclature basis, place of origin, growing environment, indications and therapeutic effects. The findings show that the ancient and modern records of the original plant for “Kui” as well as “Dongkuizi” from the family of Malvaceae is inaccurate. The original plant is creeping spinach (Basella alba, also known as Vine Spinach, Red Vine Spinach, Climbing Spinach) belonging to Basellaceae family.
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    Effects of Bushen Shengxue Jiedu Fang on lymphocyte differentiation and hematological parameters in mice with immune-mediated aplastic anemia*
    LI Xue, WEI Yi, DENG Peiying, DONG Bin, JIN Qiushuo, CHAI Limin
    2017, 40 (10):  833-838.  doi: 10.3969/j.issn.1006-2157.2017.10.008
    Abstract ( 388 )   PDF (1200KB) ( 204 )   Save
    Objective To investigate the effects of Bushen Shengxue Jiedu Fang (Kidney-tonifying blood-generating toxin-removing Formula, BSSXJDF) on lymphocyte differentiation and hematological parameters in mice with immune-mediated aplastic anemia (AA). Methods Mice in all groups except the normal group received 60Co-γirradiation combined with injection of allogeneic immune cells in the tail vein to establish bone marrow hematopoietic inhibition model. The immunosuppressive cyclosporine A was used as the positive control drug, whereas Bushen Shengxue Jiedu Fang (BSSXJDF) was given to mice in the treatment groups for 28 consecutive days. Changes of hematological parameters after medication in the peripheral blood of mice were measured with cytometer at day 7,14,21 and 28. The ratio of T, B and NK cells was detected with flow cytometer. Results The count of red blood cells (RBC), hemoglobin (HGB), platelets (PLT) and white blood cells (WBC) in peripheral blood of mice were significantly increased (P<0.05) in BSSXJDF group compared with the model group; the ratio of CD3+CD4+ cells increased(P<0.01), and the ratio of CD3-CD19+ cells and CD3-NK1.1+ cells decreased(P<0.01) with statistical significance in the cyclosporine and BSSXJDF groups compored with the model group. Conclusion Bushen Shengxue Jiedu Fang can repair the hematopoietic function of bone marrow by regulating the differentiation of lymphocytes in the treatment of AA.
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    Effects of Danzhi Yin on mice with sequelae of pelvic inflammatory disease via JNK signal transduction pathway and inflammation-related cytokines*
    XU Lin, ZHUANG Yulong, JIN Zhe
    2017, 40 (10):  839-844.  doi: 10.3969/j.issn.1006-2157.2017.10.009
    Abstract ( 361 )   PDF (1910KB) ( 213 )   Save
    Objective To investigate the mechanism of Danzhi Yin (Pony Bark Jasmine Fruit Decoction, DZY) in treating mice with sequelae of pelvic inflammatory disease (SPID). Methods 24 mice were randomly divided into the blank group, model group, DZY group and aspirin group. The SPID model was established by intrauterine infection with chlamydia muridarum. After treatment, serum levels of IL-6 and IL-8 were detected by using ELISA. The protein expression of MCP-1, MIP-2, and p-JNK were detected with Western blot. The protein phosphorylation of JNK was measured with immunochemistry technology. Results Compared with blank group, IL-6, IL-8, p-JNK, MCP-1, and MIP-2 levels in the model group were significantly higher (P<0.01). Compared with model group, IL-6, IL-8, p-JNK, MCP-1, and MIP-2 levels in the DZY group were significantly lower (P<0.01). Conclusion DZY may play an anti-inflammatory role by regulating the levels of cytokines (IL-6, IL-8) in serum. DZY may inhibit the inflammation through suppression of JNK signal transduction pathway.
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    Establishing orthotopic and metastatic colon cancer model by submucous injection of HCT116 cells in nude mice*
    WANG Rongrong, LI Yongmin, WANG Qimei, JIANG Yilan,TAN Xiaoning, JIAN Xiaolan, YANG Xiao
    2017, 40 (10):  845-848.  doi: 10.3969/j.issn.1006-2157.2017.10.010
    Abstract ( 591 )   PDF (1803KB) ( 722 )   Save
    Objective To establish a simple, feasible and reliable orthotopic implantation and metastasis model of human colon cancer in nude mice cecum mucosa. Methods Tumor cell line HCT116, derived from human colon cancer, was injected into colon cecum mucosa of nude mice to develop colorectal cancer model. HE staining and human carcinoma antigen (CEA) immunohistochemistry were performed to detect tumor in situ and metastasis condition in abdominal lymph nodes, the liver and the lungs. Results On the 2nd week after inoculation, 90% of the nude mice developed cecum tumor of 2-5 mm in diameter in situ. Abdominal lymph node metastasis was observed on the 4th week and present in all mice at week 12. Liver and lung metastasis started to appear at a percentage of 60% on the 10th week and 39% on the 12th week. Conclusion Human colon cancer in situ could be successfully established by inoculating HCT116 into cecum mucosa of nude mice to mimic the clinical development and growth of colon cancer. This method proves to be simple with a high tumor-bearing rate. It could be used as a reliable and optimal animal model for in-depth basic research about the mechanism of colon cancer metastasis.
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    Effects of qi-tonifying and blood-activating therapy on mice breast cancer model with qi-deficiency blood-stasis pattern: animal model establishment and interventional study*
    ZHENG Xiang, GUO Yong, LI Yan
    2017, 40 (10):  849-855.  doi: 10.3969/j.issn.1006-2157.2017.10.011
    Abstract ( 386 )   PDF (1443KB) ( 266 )   Save
    Objective To establish a mice model of breast cancer 4-T1 with the pattern of qi deficiency and blood stasis and study the effects of qi-tonifying and blood-activating therapy on tumor microenvironment. Methods 48 female Balb/c mice were randomly divided into 6 groups: blank control group (group A), tumor-bearing model group (group B), tumor-bearing with qi-deficiency blood-stasis pattern composite model group (group C, composite model), composite model with low-dose herbal treatment group (group D), composite model with mid-dose herbal treatment group (group E), and composite model with high-dose herbal treatment group (group F). 4-T1 monocyte suspension was injected to induce breast cancer model while reserpine injection was added to establish qi-deficiency blood-stasis composite pattern model. Herbal treatment (Si junzi Tang plus Xuefu Zhuyu Tang, i.e. four noble-ingredient decoction plus blood-activating stasis-removing decoction) of corresponding dose was offered to mice (intragastric administration) in group D, E and F from the 15th day daily for 28 days consecutively. Symptoms scores were evaluated on the 7th, 14th, 21st, 28th, 35th, and 42nd day. On the 42nd day blood flow of microcirculation in ear rim, toes and veins on the abdominal surface was detected. Matrix metalloproteinase-2 (MMP-2) and phospho-Erk1/2 (p-ERK1/2) in tumor issue were tested by using Western blot. Results Symptoms of qi deficiency and blood stasis were observed in group C, D, E and F. Symptom score of group D became lower than group C since day 28(P<0.05). The local blood flow of mice in group A was higher than that of group C fom day 21 (P<0.01). Blood flow in group C was lower than that in group B (P<0.05). Microcirculation all improved in group D, E and F compared with group B (P<0.01 or P<0.05). The expression of MMP-2 in mice in group C was higher than that in group B(P<0.05); lower in group D, E and F than that in group C(P<0.05). The expression of p-ERK1/2 in group C was slightly higher than that in group B(P>0.05); lower in group D and E than that in group C (P<0.01 or P<0.05). Conclusions The mice composite model of breast cancer with qi deficiency and blood stasis established in this study proves to be reliable and consistent with TCM pattern characteristic. MMP-2 and p-ERK1/2 were upregulated in mice tumor with the pattern of qi deficiency and blood stasis. Qi-tonifying and blood-activating herbal intervention could not only relive qi deficiency and blood stasis, but also downregulate the expression of MMP-2 and p-ERK1/2, and thus inhibit the growth of tumor.
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    Effects of Cigu Xiaozhi Wan on NF-κB mediated nonalcoholic fatty liver apoptosis*
    MA Yanhua, YANG Shaojun, BAI Zhouxia, XU Sanpeng
    2017, 40 (10):  856-863.  doi: 10.3969/j.issn.1006-2157.2017.10.012
    Abstract ( 410 )   PDF (3404KB) ( 177 )   Save
    Objective To study the effects of Cigu Xiaozhi Wan (Sagittaria Lipid-reducing Pill, CGXZW) on the expression of nuclear factor-κ gene binding(NF-κB), B-cell lymphana-2(Bcl-2) and Bcl-2 associated X protein(Bax) in rats with nonalcoholic fatty liver disease (NAFLD) and explore the therapeutic mechanism of toxin-removing phlegm-resolving method. Methods SPF grade male Wistar rats (n=60) were randomly divided into six groups: normal control group, model group, low-dose, mid-dose and high-dose CGXZW groups, and pioglitazone hydrochloride group (n=10 in each group). All rats were fed with high-fat diet to establish NAFLD model except those in the normal control group. After 6 weeks of intervention, all the experimental animals were sacrificed. Liver and serum sample were taken. Serum samples were drawn to test liver function and blood lipid by automatic biochemical analyzer. The liver histopathological changes were observed under microscope with HE staining.The ultrastructure of liver cells was observed by using transmission electron microscope. The expression of NF-κB, Bcl-2 and Bax in rats of all groups was detected by using Western blot. Results Compared with the normal group, the serum levels of alanine aminotransferase(ALT), aspartate transaminase(AST), triglyceride(TG), total cholesterol(TC) and low density lipoprotein-cholesterol(LDL-C) were significantly increased (P<0.01) in the model group; liver tissue of NAFLD rats showed moderate to severe steatosis and hepatocytes apoptosis. The expression of NF-κB and Bax in liver tissue of rats were increased significantly (P<0.01), while the expression of Bcl-2 and Bcl-2/Bax ratio were significantly reduced (P<0.01) in the model group. Compared with the model group, the serum levels of ALT, AST, TG, TC and LDL-C were decreased significantly in all-dose CGXZW groups (P<0.01); the expression of NF-κB and Bax were significantly reduced (P<0.01) whereas the expression of Bcl-2 and the ratio of Bcl-2/Bax was increased (P<0.05). CGXZW high-dose group was superior to pioglitazone hydrochloride group in terms of down-regulation of NF-κB and up-regulation the expression of Bcl-2 (P<0.01) whereas pioglitazone hydrochloride group was better than all CGXZW groups in down-regulating the expression of Bax and up-regulating the ratio of Bcl-2/Bax (P<0.01). Conclusion Sagittaria Lipid-reducing Pill can significantly improve liver function and reduce blood lipid level in NAFLD rats, upregulate the expression of Bcl-2, decrease the expression of NF-κB and Bax, and inhibit liver cell apoptosis. It could be used in the prevention and treatment of NAFLD.
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    Determination and isolation of chemical constituents from the dried stems of Rubus amabilis Focke*
    DIAO Yulin, ZHU Dongqing, LIU Bin
    2017, 40 (10):  864-871.  doi: 10.3969/j.issn.1006-2157.2017.10.013
    Abstract ( 443 )   PDF (2148KB) ( 261 )   Save
    Objective To study the chemical constituents from the dried stems of Rubus amabilis (Focke). Method The chemical constituents of R. amabilis were extracted with boiled water, then isolated and purified with various chromatographic columns including silica gel, reversed phase and gelatin chromatography and crystallization methods. The structure was identified by using different spectroscopic and chromatographic techniques including nuclear magnetic resonance and mass spectrum. The liquid chromatography-mass spectrometry-ion trap-time of flight (LC/MS-IT-TOF) method was also applied to analyze the ingredients. The chromatographic separation was performed on SunFireTM C18 column (4.6 mm×150 mm, 5 μm) with gradient elution of methanol (A)-0.5% formic acid aqueous solution (B). Multistage mass spectrometry data of different compounds was obtained by using electrospray ionization source and monitoring in positive and negative ion modes. Results There were 5 chemical constituents isolated from the plant including 1, 2, 8-trimethoxy-6-hydroxyxanthone, 2α, 3α, 19α, 23-tetraydroxy-urs-12-en-28-oic acid, oleanolic acid, (+)-catechin and (-)-epicatechin. Twenty-seven compounds including 5 diterpenoids, 1 monoterpenoid, 1 xanthone, 11 flavones and 9 triterpenoids were identified based on the characteristic MS/MS fragment ions data with chemical standards and published literatures. These constituents were determined to be 1-hydroxyl-3,7,8-trimethoxylxanthone, 7-epiphlomiol, ent-kaur-16-ene-9-ol, ent-16β,17-dihydroxy-kauran-19-oic acid, ent-kauran-3α(β),16β,17-triol, rubusoside, rubusoside B, pinfaenoic acid, isopinfaenoic acid, 19α-hydroxylursolic acid, 2α,19α-dihydroxyl-ursan-12-en-28-oic acid, 2α-hydroxylursolic acid, 2α,3α-dihydroxyl-oleanan-12-en-28-oic acid, camelliagenin A, camelliagenin C, tormentic acid, procyanidin B1-B8, isoquercitrin, quercetin and kumata kenin. Conclusion Compound 1 was isolated from Rosaceae for the first time. Compounds 20 and 21 were obtained from Rubus firstly. The rest constituents were firstly found from the plant.
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    Effects of electro-acupuncture on hippocampal CXCR4 and IP-10 protein expression in rats with depression disorder*
    LIANG Xingchen, JIANG Huili,ZHANG Danmei, SHI Rongxing, WANG Yu, LI Jing, YANG Xinjing, ZHAO Bingcong, YU Qiuyun, LI Xiang, FENG Shixing, TU Ya
    2017, 40 (10):  872-875.  doi: 10.3969/j.issn.1006-2157.2017.10.014
    Abstract ( 350 )   PDF (1176KB) ( 203 )   Save
    Objective To investigate the effect of electro-acupuncture (EA) on hippocampal chemokine cys-x-cys receptor 4 (CXCR4) and interferon-inducible protein-10 (IP-10) in rats with depression disorder. Methods The rat model of depression disorder was induced by unpredictable chronic-mild stimulation. Forty male rats were randomly divided into normal, model, EA and fluoxetine groups. Rats in EA group received electro-acupuncture at GV20 (Baihui) and GV29 (Yintang). Rats in fluoxetine group were administered with fluoxetine by oral gavage. On the 28th day, the expression of hippocampal CXCR4 and IP-10 were detected by using antibody microarrays technology. Fold change and ratio of the density of the proteins were used to investigate alteration of the differential protein expression. Results Compared with the normal group, unpredictable chronic-mild stimulation up-regulated the expression of hippocampal CXCR4 and IP-10 in model group (fold change=1.21,1.50). Compared with the model group, EA and fluoxetine reversed the up-regulation of hippocampal CXCR4 and IP-10 (EA group: fold change=0.65, 0.72; fluoxetine group: fold change=0.57, 0.62). Conclusion Effects of electro-acupuncture on depression disorder may be achieved by regulating the expression of hippocampal CXCR4 and IP-10.
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    Correlation between clinical manifestations and pattern diagnosis of primary liver cancer based on association rules*
    SHI Huayue, WU Chengyu
    2017, 40 (10):  876-880.  doi: 10.3969/j.issn.1006-2157.2017.10.015
    Abstract ( 373 )   PDF (1232KB) ( 213 )   Save
    Objective To investigate the correlation between clinical manifestations (symptoms and signs) and pattern (disease location and nature) diagnosis in liver cancer based on association rules. Methods 1 042 patients with primary liver cancer were identified by using clinical epidemiological method. Medcase V3.2 was used to set up the dataset for the analysis of association between manifestations and patterns in liver cancer. Results Clinical manifestations such as right hypochondriac pain, upper right abdominal pain, abdominal distension, abdominal bulge, ascites, jaundice, loss of appetite, fatigue, greasy coating, and wiry pulse were more common in patients with liver cancer. The patterns of primary liver cancer appeared to be located mainly in the “liver, spleen, and gallbladder”, and were closely related to “qi deficiency, qi stagnation, blood stasis, and dampness”. Conclusion Correlation between clinical manifestations and patterns in liver cancer is present based on association rules. Given the ambiguity and complexity of TCM data, different data mining algorithms should be developed and applied to benefit TCM clinical practice.
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