Influence of total flavonoids in Shayuanzi on blood fat level and synthesis pathway of triglyceride in rats with hyperlipidemia (kidney yang deficiency pattern)*
2018, 41 (1):
Objective To investigate the therapeutic effect of total flavonoids in Shayuanzi (Semen Astragali Complanati, flatstem milkvetch seed, TFS) on hyperlipidemia (kidney yang deficiency pattern) and their influence on liver synthesis pathway of triglyceride (TG). Methods SD rats (10 weeks old) were divided, according to weight randomized block method, into 6 groups: sham-operation group, model group, estrogen group, high-dose TFS group, mid-dose TFS group) and low-dose TFS group (each n=10). The rat model of hyperlipidemia (kidney yang deficiency pattern) was copied by bilateral ovariectomization and high fat diet for 6 weeks. The sham-operation group and model group were intragastrically given normal saline (10 mL/kg), estrogen group, estradiol (0.2 mg/kg), and TFS groups, total flavonoids in Shayuanzi (28.5 mg/kg, 57 mg/kg and 114 mg/kg respectively) for 8 weeks. The levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) were detected. The activities of liver fatty acid synthase (FAS) and acctyl-CoA carboxylase (ACC), and levels of GPAT, CPT-1α and acy-CoA oxidase (ACO) were detected by using enzyme immunoassay (EIA). The protein expressions of sterol regulatory element-binding protein-1c (SREBP-1c) and peroxisome proliferator-activiated receptor-α (PPAR-α) were detected by using immunohistochemistry technique. ResultsCompared with model group, the levels of serum TG, TC and LDL-C decreased significantly (P<0.05, P<0.01), level of serum HDL-C increased significantly (P<0.05), activities of liver ACC and GPAT level decreased significantly (P<0.01, P<0.01), levels of ACO and CPT-1α increased significantly (P<0.01, P<0.05), protein expression of SREBP-1c was significantly down-regulated, and protein expression of PPAR-α was significantly up-regulated in high-dose TFS group. Conclusion TFS has a good effect of regulating blood lipid metabolism, which is its major material base of regulating blood fat. The mechanism may be related to that TFS can inhibit the expression of liver SREBP-1c, reduce the activities and levels of FAS, ACC and GPAT in TG synthesis pathway, and up-regulate protein expression of PPAR-α, ACO and CPT-1α for inhibiting liver TG synthesis and controlling blood fat level.
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