主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    28 February 2018, Volume 41 Issue 2 Previous Issue    Next Issue
    A review on Huangdi Neijing’s contribution to critical disease management in TCM emergency discipline*
    Wang Xiaopeng, Chen Tengfei, Liu Qingquan
    2018, 41 (2):  93-96.  doi: 10.3969/j.issn.1006-2157.2018.02.001
    Abstract ( 485 )   PDF (1256KB) ( 522 )   Save
    Huangdi Neijing (Huangdi’s Internal Classic, hereinafter referred to as Neijing) not only laid the foundation of traditional Chinese medicine theories, but also recorded quite a few data about critical diseases. Neijing categorized and named critical diseases, and most of those diseases were named as Jue (syncope), bao (sudden onset), and cu (sudden death), which built a series of basic pathogenesis theories for TCM critical diseases. The statements of yin-yang relationship in Neijing illuminated the pathogenesis of critical disease to a large extent. Neijing explicitly recorded manifestations, diagnosis thinking pattern, treatment principles, and therapies of critical diseases. Neijing marks the establishment of TCM emergency system, and provides basic theories for TCM emergency.
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    Bigu is not a TCM life nurturing method for healthy people*
    Ma Fangfang, Liao Yan, Lin Yin, Zhang Cong, Xi Xi, Pan Shixia, Wang Yichen, Ke Xiuhui
    2018, 41 (2):  97-101.  doi: 10.3969/j.issn.1006-2157.2018.02.002
    Abstract ( 483 )   PDF (1193KB) ( 599 )   Save
    No ancient records of bigu (abstinence from grains) for life nurturing in healthy people have been identified although it was originally a taoist asceticism for pursuing immortality. The TCM classics of Huangdi Neijing and Nanjing have shown that healthy person with bigu would die of losing spirit after seven days. Bigu and life nurturing have been divided into different chapters to show the difference in the ancient medical books. The taoist doctors oppose bigu on account of early death and malady. Shiqi Bigu(eating qi with abstinence from grains) lacks theoretical evidence and no success cases could be verified. “Bloating without appetite” originally refers to the symptom (anorexia) of spleen deficiency pattern. This term is used in Taoist practice to denote willpower of “eliminating desire” (zhinian) in taoist cultivation. Without precise fabrication, bigu prescriptions tend to consist of 80% sweet-flavored ingredients or grain and tonic herbs, which may be the actual cause of bloating and anorexia with safety concern. Bigu seems to have deviated from the ancient rule of TCM “well-balanced diet with the grains, meats, fruits and vegetables for health”, and also violated the principle of “food variety with grain dominant” diet in modern nutrition. There is no rationality to include bigu into the life nurturing methods or therapies of traditional Chinese medicine.
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    Changes of estrus in female rats with liver-stagnation pattern and effects of Chaihu Shugan San on its prevention and treatment*
    Zhang Yuanfeng,Qian Meng,Liu Biyuan,Xie Ming
    2018, 41 (2):  102-109.  doi: 10.3969/j.issn.1006-2157.2018.02.003
    Abstract ( 421 )   PDF (1436KB) ( 254 )   Save
    Objective To investigate the changes of estrous cycle and the preventive and therapeutic effects of Chaihu Shugan San (Radix bupleuri liver-soothing Powder, CHSGS) in female rats with liver-stagnation pattern. Methods Female rats were randomly divided into normal group, liver-stagnation group, high-dose CHSGS group (H group), and low-dose CHSGS group (L group) (n=10 in each group). Liver stagnation pattern was established by using chronic restraint stress for 4 weeks. After modeling for 14 days, rats in the H and L groups were given the investigated medicine at the dose of 1.26 g/kg and 0.31 g/kg (equivalent to 2 and 0.5 times of human dosage) respectively, once a day for 2 weeks. Rats in the model group and the normal group were given the same amount of distilled water. At week 2 and week 4, sucrose preference test (SPT) was conducted; At week 4, open field test (OFT) was performed. Rat estrous cycles were observed by using vaginal smears. General observation was used throughout the experiment. Results At week 2, compared with the normal group, the model group showed a state of hyperactivity, irritability, reduced and slower mobility, and reduced food-intake. SPT were higher at week 2 but decreased at week 4 (P<0.01). Estrous cycle disorders were also reduced (P<0.01). Duration in central zone (Index S), cubicle-crossing counts (Index C) and vertical movements (Index N) decreased in OFT test (P<0.01). Compared with the model group, the activities of the rats in the H group were more similar to the control group, with the duration in the central zone and vertical movements higher than those in the model group (P<0.01) and SPT decreased (P<0.01). Disordered estrous cycles decreased in H group (P<0.01). The above-mentioned changes were not observed in the L group (P>0.05). Conclusion Behavior changes with estrous cycle disorders were observed in female rat with liver-stagnation model established by chronic restraints stress. Chaihu Shugan San appeared to be effective in relieving the symptoms and estrous cycle disorders. High-dose CHSGS was superior to low-dose CHSGS.
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    Intestinal uric acid excretion in rats with hyperuricemia*
    Wang Yu, Lin Zhijian, Zhang Bing
    2018, 41 (2):  110-117.  doi: 10.3969/j.issn.1006-2157.2018.02.004
    Abstract ( 343 )   PDF (1767KB) ( 330 )   Save
    Objective To explore the pathological mechanism of hyperuricemia by observing the alteration of intestinal uric acid excretion and intestinal transporter ABCG2 expression in rats with hyperuricemia induced by fructose. Methods SD male rats (n=24) were randomly divided into normal group and model group according to the weight. The rats in the normal group were given water, and the rats in model group were given 10% fructose solution to establish hyperuricemia model. Levels of serum uric acid (SUA) and fecal uric acid (FUA) were measured every 10 days. The intestinal urate excretion (IUE) level was detected by using in situ perfusion method and HPLC. The location and expression of transporter ABCG2 in the intestine was analyzed with immunohistochemical staining and semi-quantitative analysis of protein was conducted. Protein expression of transporter ABCG2 in each part of intestine was further detected by using Western blot. The expression of mRNA of ABCG2 in both groups was determined with RT-qPCR. Results From the 20th to the 40th days, SUA in the model group was significantly higher than that in normal group. From the 10th to the 30th days, the FUA in model group was significantly lower than that in normal group. The IUC in model group was significantly lower than that in normal group. Immunohistochemical staining showed that the intestinal transporter ABCG2 was located in the intestinal villus and gland, and the analysis of staining showed that the protein expression of ABCG2 in the jejunum and ileum was significantly lower than those in the normal group. Western blot tests exhibited the same results. RT-qPCR indicated that compared with the normal group, expression of mRNA of ABCG2 in the jejunum and ileum in the model group were markedly lower. Conclusion Fructose could successfully induce hyperuricemia in rats. Hyperuricemia induced by fructose presents dysfunction in the intestinal uric acid excretion, which is probably related to the down-regulation of the ABCG2 mRNA level and inhibition of ABCG2 protein level in the jejunum and ileum.
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    Effects of kidney-supplementing and blood-activating formula on Wnt/β-catenin signal pathway of stem cell of thin endometrium*
    Yin Xiaodan, He Junqin, Yang Wei, Xin Mingwei, Xue Xiao’ou
    2018, 41 (2):  118-123.  doi: 10.3969/j.issn.1006-2157.2018.02.005
    Abstract ( 382 )   PDF (1405KB) ( 217 )   Save
    Objective To investigate the effects of Bushen Huoxue Fang kidney-supplementing and blood-activating formula (BSHXF) on Wnt/β-catenin signal pathway in stem cell of thin endometrium. Methods During the 7th to 9th day after ovulation, 100 mg endometrial membrane samples of selected patients with thin endometrium were taken with disposable endometrial sampling device. After washing with saline, the samples was fixed in 4% polyformaldehyde and then separated to culture the endometrial stem cells, and drug serum was prepared. The cultured endometrium stem cells were divided into the control group, High, medium and low dose groups of kidney-supplementing and blood-activating formula (BSHXF) and Western medicine group. After intervention, β-catenin /TCF (T cytokine) and transcriptional activity were measured by using double Luciferase Report System; CD34/CD117 protein expression was examined with immunofluorescence; Oct-4 gene was examined with RT-PCR; and ABCG2 protein was measured by using Western blot. Results After transfecting the same dosage of plasmid, there was a significant difference among mid-dose BSHXF group, high-dose BSHXF group, Western medicine group, and control group (P<0.05). CD34/CD117, Oct-4 gene and ABCG2 protein had positive expression in the endometrium stem cells. There was a significant difference in the expression of CD34 protein between BSHXF groups and control group (P<0.05). There was a significant difference between high-dose BSHXF group and Western medicine group (P<0.01). The expression of CD117 protein in the high-dose group was significantly different from that in the control group (P<0.05), and there was a significant difference between the Western medicine group and the control group (P<0.01). RT-PCR test showed that the expression rate of Oct-4mRNA were significantly different from the interventional groups and the control group (P<0.05). Western blot test showed that the expression rate of ABCG2 protein in the interventional groups were significantly different from that in the control group (P<0.05). Conclusion Kidney-supplementing and blood-activating formula can increase the fluorescent activity of thin endometrial stem cells, thereby promote the activation of Wnt/β-catenin signal pathway, and increase the expression of CD34/CD117, OCT4 gene and ABCG2 protein.
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    Effects of baicalein on AC-cAMP-PKA signaling pathway in prefrontal cortex of rats with attentional deficit hyperactivity disorder*
    Wang Qing, Fan Jinping, Liao Yingzhao, Chen Jing, Liu Hua, Ni Xinqiang
    2018, 41 (2):  124-130.  doi: 10.3969/j.issn.1006-2157.2018.02.006
    Abstract ( 391 )   PDF (1161KB) ( 233 )   Save
    Objective To study the effects of baicalein on spontaneous activity, impulsivity, learning-memorial ability, and the adenylate cyclase (AC)-cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) signaling pathway in prefrontal cortex (PFC) of rats with attention deficit hyperactivity disorder (ADHD). Methods Fifty 4-week-old SHR rats were randomly divided into the model group, methylphenidate (MPH) group (2 mg/kg), and low-dose, mid-dose and high-dose (100, 200, 400 mg/kg) baicalein groups, and WKY rats of the same age were selected as the normal control group (n=10 in each group). Rats were orally gavaged for 4 weeks in the dark-phase. Open field test (OFT), elevated plus maze (EPM) and novel object recognition test (NORT) were conducted to evaluate the spontaneous activity, impulsivity and learning-memorial ability of rats individually at the end of treatment. AC, cAMP and PKA quantity were determined with enzyme-linked immunosorbent (ELISA) method. Results Movement distance in OFT was reduced in high-dose baicalein group and mid-dose baicalein group (P<0.05) afterwards. Vertical movements also decreased in all baicalein groups. Percentage of the times entering the open arms in the EPM was significantly reduced in the mid-dose baicalein group (P<0.05); staying duration in the total period in the EPM was also reduced in all baicalein groups(P<0.05). The preference index in the testing phase of NORT increased in the mid-dose baicalein group (P<0.05). Levels of AC and cAMP in the PFC increased in all baicalein groups (P<0.05), while tPKA increased in high-dose and mid-dose baicalein group (P<0.05). Conclusion Baicalein could increase the levels of AC, cAMP and PKA in PFC of ADHD rats, which may shed some light to the mechanism how baicalein could relieve the spontaneous activity and impulsivity, improve learning-memorial ability of ADHD rats.
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    Lipid-lowering ingredients in Chinese medicinals based on PPARγ-LXRα-ABCA1 pathway*
    Zhao Bowen, Chen Yankun, Zhang Xu, Gu Yu, Zhang Yanling
    2018, 41 (2):  131-139.  doi: 10.3969/j.issn.1006-2157.2018.02.007
    Abstract ( 439 )   PDF (3180KB) ( 312 )   Save
    Objective To discover the potential compounds with lipid-lowering` effect in Chinese medicinal based on PPARγ-LXRα-ABCA1 pathway by using pharmacophore and molecular docking technology. Methods Firstly, PPARγ and LXRα were selected as search keywords, and the efficacy-identifying models of their agonists were built respectively. The optimal pharmacophore models of PPARγ and LXRα were obtained after validation and evaluation. Traditional Chinese medicine database (TCMD) was selected according to the optimal pharmacophore models. The primary screening compounds were retained based on the Lipinski five rules and Fitvalue. Finally, molecular docking was employed to refine the outcomes of pharmacophore model and analyze the binding mode of protein-ligand. The compounds with higher docking scores and amino acids were retained. Results In this study, the optimal model of PPARγ agonists was built, which contains one hydrogen bond acceptor, one hydrophobic group and two aromatic rings; The optimal model of LXRα agonists contains two hydrogen bond acceptors, one hydrophobic group and two aromatic rings. Meanwhile, the crystal structure of PPARγ and LXRα built by molecular docking method was used in molecular docking study. According to the pharmacophore and outcomes of Lipinski five rules, there were 22 and 180 compounds obtained respectively. Based on the screening standard of docking studies, the artabotrycinol and hinoki flavone were identified as potential agonists of PPARγ, while anomalamide (artemisia annua) and trichosanatine (trichosanthes rosthornii) were identified as potential agonists of LXRα. These potential compounds could play a key role by acting on the corresponding target in PPARγ-LXRα-ABCA1 pathway respectively. Conclusion This study provides an efficient method for screening potential agonists of PPARγ and LXRα in TCMD and potential compounds that increase the expression of ABCA1, which could serve as the candidate compounds in the development of new drugs for hyperlipidemia.
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    Network pharmacological studies of Mongolian compound formula Xieriga-4*
    Bai Feng, Li Binxin, Dong Yu, Zhang Tianming
    2018, 41 (2):  140-147.  doi: 10.3969/j.issn.1006-2157.2018.02.008
    Abstract ( 390 )   PDF (2877KB) ( 304 )   Save
    Objective This study aims to explore the active ingredient and action target of Mongolian medicine compound formula Xieriga-4 in the treatment of benign prostatic hyperplasia, prostate cancer and other diseases. The mechanism of the chemical composition of Xieriga-4 was analyzed by using network pharmacology technology. Methods By searching China National Knowledge Infrastructure (CNKI), Wanfang database and other databases, Mongolian compound formula Xieriga-4’s composition was identified. PubMed, Super Target, Drug Bank, TTD (Targets Database Therapeutic) and other database were used to search for action targets of the chemical composition of Xieriga-4; KEGG (Kyoto Encyclopedia of Genes and Genomes), IPA (Ingenuity Pathway Analysis)and other database were searched to explore relevant signal pathway; Cytoscape software was applied to establish the chemical constituents-target and chemical composition-target-disease network model of the Mongolian compound formula Xieriga-4. Results The correlation between drug and target provided a new way to explore potential targets. The screening results showed that Mongolian drug compound formula Xieriga-4 has network interaction in 33 compounds and 12 kinds of biological targets, the correlation among which implied the “multi-component”,“multi-target” characteristic of online pharmacology. Meanwhile the compounds and target sites act together on disease-benign prostatic hyperplasia and prostate cancer, and the Mongolian compound Xieriga-4-chemical composition-target-disease network mode could thus be established. Through the database searching, two major signal pathways were identified, i.e. AR signal pathway and Wnt/-catenin signaling pathway, which provided a new idea for Mongolian medicine to explore the mechanism of disease treatment. Conclusion This study has established a “disease-target-drug” model by using internet-based pharmacology, which could be a new approach to the research and development of Mongolian medicine.
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    Influence of high frequency electroacupuncture on skin temperature around meridian acupoints, and non-menridian sham acupoints in rats with myocardial ischemia*
    Bai Hongxin, Ji Bo, Zhao Guozhen, Wang Dan, Yan Mingna, Sun Xiaomin, Lu Yawen, Dai Jian, Liu Yitian, Ge Yunpeng, Su Hang, Wu Jiaojuan, You Min, Chen Kai, Li Zhigang
    2018, 41 (2):  148-153.  doi: 10.3969/j.issn.1006-2157.2018.02.09
    Abstract ( 329 )   PDF (2028KB) ( 238 )   Save
    Objective To compare the skin temperature around relevant and irrelevant meridian acupoints, or non-meridian sham acupoints in rats with myocardial ischemia by high frequency electro-acupuncture (EA) at Neiguan (PC6) point or sham acupoint, in healthy rats and myocardial ischemic rats. Methods Fifty male Wister rats were randomly divided into five groups: blank control group, sham-operation group, model group, high frequency EA at Neiguan group (PC6) and high frequency electro-acupuncture at sham acupoint groups. Rats in high frequency EA at and at sham acupoints group received EA intervention after their models were successfully established. Temperature around the skin of Neiguan (PC6), Yanglingquan (GB34) and sham acupoints were measured and recorded by infrared thermography in every group at 30 minutes 60 minutes after three-day treatment for statistical analysis. Results Compared with the blank control group, skin temperature around PC6 in model groups was significantly lower (P<0.01); Compared with the model group , skin temperature around PC6 in high frequency EA at PC6 group was significantly increased (P<0.01);Skin temperature around PC6, GB34 and sham acupoint in high frequency EA at sham acupoint groups presented with no statistical significance; skin temperature around PC6 in high frequency EA at PC6 group was similar to the skin temperature of blank control group, which was measured 30 minutes after the treatment. Conclusion The relative specificity of skin temperature around PC6 can be useful for the reflection of different conditions of rats’ myocardium. High-frequency electro-acupuncture at PC6 can prevent the reduction of skin temperature caused by myocardial ischemia injury, of which the mechanism needs further study.
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    Effects of electroacupuncture on the expression of caspase-12 in hippocampus and serum of rats with chronic unpredictable mild stress*
    Tian Zhaoyang, Tu Ya, Jiang Huili, Zhang Xuhui, Lu Jun, Yu Qiuyun, Liang Xingchen, Shao Shujun, Xiang Dulian, Wu Jihong
    2018, 41 (2):  154-159.  doi: 10.3969/j.issn.1006-2157.2018.02.010
    Abstract ( 344 )   PDF (1146KB) ( 213 )   Save
    Objective To observe the effects of electroacupuncture (EA) on the expression of central and peripheral cysteine aspartic acid protease-12 via JNK signal transduction pathway in rats with chronic stress depression and to explore anti-depression mechanism of electro-acupuncture. Methods SD rats were randomly divided into blank group, stress model group, acupuncture group, fluoxetine group, model+DMSO (dimethyl sulfoxide, soluble organic solvent) group, model+JNK pathway blocker SP600125 (hereinafter abbreviated as SP) group, acupuncture+SP group, and fluoxetine+ SP group (n=9 in each group). The combination of chronic stress and solitary raising was used to establish chronic unpredictable mild stress model in seven groups except the control group. The investigated intervention was EA at “Neiguan(PC6)” and “Sanyinjiao(SP6)” points, 20 min for each session, once a day, for 21 consecutive days. The positive control group was treated with fluoxetine 5 mg/kg by oral gavage for 21 days. Open field test was used to observe the rats’ behaviors. The expression of caspase-12 protein in hippocampus and serum was measured by using ELISA. Results Before the experiment, there was no statistic difference in rat behaviors of all groups (P>0.05). At Day 21 of the experiment, the horizontal crossing lattice and frequency of vertical movements markedly decreased compared to the blank group (P<0.01). Compared with the model group, EA increased the horizontal crossing lattice (P<0.05). Compared with the blank group, the expression of caspase-12 protein in the model group increased significantly (P<0.01). Compared with the model group, the expression of caspase-12 protein in the model+DMSO group, fluoxetine group, fluoxetine+SP group, acupuncture group and SP group all decreased (P<0.05). Compared with the blank group, the expression of caspase-12 protein in the model group decreased. Compared with the model group, the expression of caspase-12 protein in the model+DMSO group, model+SP group, fluoxetine group and acupuncture+SP group (P> 0.05) decreased, while the expression of caspase-12 in fluoxetine+SP group and acupuncture group was higher than that in the control group, but all the differences were not statistically significant (P>0.05). Conclusion Acupuncture treatment may reduce the expression of caspase-12 protein in the hippocampus of chronic stress rats by effectively inhibiting the JNK signal transduction pathway activity and reducing the apoptosis of hippocampal neuron, which contributes to its anti-depression mechanism.
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    Emission of fine particulate matter, CO, SO2 , and NO2 during moxa’s combustion in different moxa device*
    Hui Xin, Wang Hao, He Rui, Li Tanjiao, Ha Lue, Zhang Rui, Lin Yao, Li Dan, Zuo Yingzhu, Han Li, Zhao Baixiao
    2018, 41 (2):  160-164.  doi: 10.3969/j.issn.1006-2157.2018.02.011
    Abstract ( 357 )   PDF (1130KB) ( 478 )   Save
    Objective By observing the emission of particles and various gases under different combustion conditions during moxibustion, to further discuss the safety of moxibustion therapy. Methods Eighteen moxa sticks (1 g) from the same batch were put into three types of moxibustion devices and burnt out, which was detected by laser measuring dust and gas detectors. These moxa sticks were randomly divided into natural exposure group, moxa cylindrical device group and moxa cylindrical device with filter group (n=6 in each group). Ignition was made in the sealed glass room, and PM2.5 (Particulate Matter 2.5) mass concentration, CO, SO2 and NO2 emissions were constantly monitored, and peak values of each one were noted. Results There were statistic differences among the three groups in PM2.5, CO and SO2 levels. The concentration of PM2.5 and the emission of CO and SO2 in group of moxa cylindrical device with filter were the lowest in the three groups (P<0.05). Moxibustion did not affect the content of NO2. The peak concentration of PM2.5 in descending order was: moxibustion cylindrical device group>natural exposure group>moxibustion cylindrical device with filter group. The peak emission values of CO and SO2: natural exposure group > moxibustion cylindrical device group > moxibustion cylindrical device with filter group. Conclusion There were differences in the emission of PM2.5, CO and SO2 in different devices. Controlling the moxa burning conditions can reduce the emission of PM2.5, CO and SO2. Therefore physical barrier measures should be taken to effectively reduce the concentration of PM2.5 in moxibustion sites and avoid moxa smoke.
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    Distribution of pattern elements and target organs in dyslipidemia: factor analysis of 1019 cases*
    Yang Tao, He Qingyong, Zhong Xiaoxue, Liu Xudong, Wang Hui, Guo Jianbo, Ding Yukun
    2018, 41 (2):  165-170.  doi: 10.3969/j.issn.1006-2157.2018.02.012
    Abstract ( 355 )   PDF (1140KB) ( 293 )   Save
    Objective To explore the distribution of pattern elements and target organs in dyslipidemia aiming at providing theoretical basis for dyslipidemia diagnosis and treatment in clinical practice. Methods A database matrix including clinical manifestations (containing 89 symptoms, physical signs, tongue and pulse) of 1019 patients with dyslipidemia. Factors were extracted according to initial models and rotated with Varimax, then the factor loading and the score of common factors was calculated. Pattern elements were concluded by the common factors, and the patients were grouped by the score of each pattern element. Thus the distribution of pattern elements and target organs were assigned according to its score of each group. Results 38 common factors and 14 pattern elements were extracted, from which 6 primary pattern elements were selected, including 3 excessive elements, phlegm (284 cases, 25.08%), blood stasis (130 cases, 11.48%), and heat (109 cases, 9.62%) and 3 deficient elements, qi deficiency (170 cases, 15.00%), yang deficiency (164 cases, 14.47%), and yin deficiency (108 cases, 9.53%). 8 target organs were extracted in which the main target included the spleen (508 cases, 40.38%) , lung (269 cases 21.38%), heart (218 cases, 17.33%) and liver (190 cases, 5.25%). Conclusion s Dyslipidemia in traditional Chinese medicine belongs to deficiency in its root (nature) and excess in branch (manifestation). Excessive manifestations include phlegm, blood stasis and heat, and deficient nature includes qi deficiency, yang deficiency and yin deficiency. The main symptom target (location) was the spleen, the lung, the heart and the liver. Therefore, the findings suggest that treatment of dyslipidemia in traditional Chinese medicine could rely on resolving phlegm, activating blood, removing heat, tonifying qi, warming yang, and reinforcing yin. Spleen should be considered as the first and foremost organ in the treatment, while the lung, heart, and liver should also be taken into consideration.
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    Effects of heat-sensitized moxibustion combined with conventional medicine on knee osteoarthritis of yang-deficiency cold-congealing pattern: a clinical trial*
    Wang Zhiqin, Xie Hongwu, Liu Fushui, Jiao lin, Chen Rixin
    2018, 41 (2):  171-176.  doi: 10.3969/j.issn.1006-2157.2018.02.013
    Abstract ( 347 )   PDF (1143KB) ( 257 )   Save
    Objective To explore the effects of heat-sensitized moxibustion combined with conventional medicine on knee osteoarthritis (KOA) including clinical efficacy and cytokine levels. Methods 60 patients with bilateral knee osteoarthritis was divided into two groups (n=30). The treatment group was given heat-sensitized moxibustion combined with conventional drugs, while the control group was administered with routine acupuncture treatment besides conventional drugs. Bilateral ST35, GB34, and EX-LE2 were selected as treating acupoints for 15 minutes once a day for 6 days as one treatment session. Four treatment sessions were one total treatment course, with 1-day interval between two sessions. The Lysholm knee function score was applied to evaluate clinical efficacy before and after treatment. The scores of joint pain, joint swelling, and walking ability and cytokine TNF-α/IL-1 were also compared before and after treatment. Criteria of adverse effect factors was adopted to evaluate the safety.Results The scores of joint pain, joint swelling, walking ability and serum cytokines TNF-α/IL-1of patients in both groups were improved after treatment (P<0.05). Compared with the control group, those above index in the treatment group were improved more markedly (P<0.01). The effective rate of the treatment group and control group was 86.7%, and 66.7% respectively, thus the efficacy of treatment group was significantly superior to that of the control group (P<0.01). Moreover, compared with control group, there were less adverse effects in treatment group (P<0.05). Conclusion To treat knee osteoarthritis, heat-sensitized moxibustion appears to be superior to routine moxibustion therapy in terms of efficacy and safety. The non-invasive heat-sensitized moxibustion may alleviate symptoms, and improve physical signs effectively, which warrants further exploration and development.
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