Effects of Cyclocarya Paliurus leaves aqueous extract on pancreatic β cell’s apoptosis in type 2 diabetic rats*
2018, 41 (8):
Objective To investigate the effects of Cyclocarya Paliurus aqueous extract (CPAE) on pancreatic β cell apoptosis in type 2 diabetic (T2D) rats. Methods T2D ract model was established by high-fat diet feeding for 8 weeks and single intraperitoneal injection of streptozotocin. From the ninth week, the rats in the model group (sodium chloride solution), metformin group (0.3 g/kg), low, medium and high dose CPAE groups (0.18, 0.36, 1.08 g/kg) were treated for 4 consecutive weeks. Glucose tolerance was measured at the last administration. After sacrifing the rats and removing the pancreas, the contents of superoxide dismutase (SOD), peroxidase (CAT), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and free fatty acid (FFA) were detected with colorimetric method. Tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) were measured with radioimmunoassay. The levels of apoptosis factor (Fas) and its ligand (Fasl), activated cysteine protease (Caspase8) were detected by using ELISA. Western blot assay was used to detect total c-Jun N-terminal kinase 1 (T-JNK1), phosphorylated c-Jun N-terminal kinase 1 (P-JNK1), pancreatic duodenal homeobox 1 (PDX-1) protein expression; TUNEL staining was used to observe pancreatic cell’s apoptosis. Results CPAE treated groups presented with increased contents of SOD, CAT, GSH-PX and increased expression of PDX-1 in the pancreas of the CPE groups; fasting blood glucose, area under the curve, MDA, FFA, TNFα, IL6, Fas, Fasl, Caspase8 content, T-JNK1, p-JNK1 expression, and pancreatic cell apoptosis rate were all reduced in the model group. Conclusion CPAE can improve the pancreas antioxidant, alleviate oxidative stress, and inhibit pancreatic β cell’s apoptosis. The mechanism may be related to inhibiting the expression of T-JNK1 and P-JNK1 protein, increasing the expression of PDX-1 protein and decreasing the content of Fas, Fasl and Caspase8.
Related Articles |