Maka, its TCM properties and influence on mitochondrial energy metabolism enzymes in spleen deficiency mice*
2018, 41 (7):
Objective To investigate the effects of relieving physical fatigue and anti-oxidative damage of Maka (Maca, Lepidium meyenii Walp.), to verify Maka’s properties of warming and fortifying spleen, and to discuss the mechanism of energy metabolism in mice with spleen deficiency pattern. Methods Male Kunming mice were randomly divided into normal group, model group, ginseng group (control group) and high-dose, mid-dose and low-dose Maka groups (1.5 g/kg, 0.75 g/kg, 0.25 g/kg). Except of normal group, other groups were given abnormal diet and fatigue to impair spleen for 14 d for establishing mouse model of spleen deficiency pattern. Meanwhile all groups were preventively given corresponding drugs at the first day. The time of forced swimming test (FST) was recorded in spleen deficiency mice. The activities or concentration of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidease (GSH-PX) and malondialdehyde (MDA) in liver tissue homogenate were detected by using chromatoptometry. The concentration of serum cAMP and cGMP were detected by using radioimmunoassay (RIA), and ratio of cAMP to cGMP (cAMP/cGMP) was calculated. The activity of F0F1-ATP enzyme was detected by using continuous cycle method, and activities of Na+-K+-ATP and Ca2+-Mg2+-ATP enzymes were detected by using enzymolysis. Results Compared with normal group, mouse body weight and time of FST decreased, activities of SOD, CAT and GSH-PX decreased, MDA content increased, cAMP/cGMP decreased, and activities of F0F1-ATP, Na+-K+-ATP and Ca2+-Mg2+-ATP decreased in model group (P<0.01). Compared with model group, mouse body weight was increased in all Maka groups (P<0.01). The time of FST was significantly prolonged in high-dose Maka group and ginseng group (P<0.01), which was not significant in mid-dose and low-dose Maka groups. The activities of SOD, CAT and GSH-PX were significantly increased (P<0.01), MDA content was decreased (P<0.01), cAMP/cGMP was significantly increased (P<0.01), and activities of F0F1-ATP, Na+-K+-ATP and Ca2+-Mg2+-ATP were significantly increased (P<0.01) in mid-dose and high-dose Maka groups, which was no significant in low-dose Maka group. Conclusion Maka, in a certain dose, can relieve physical fatigue, improve activities of anti-oxidative enzymes and eliminate cytotoxicity of free radicals, which verifies Maka’s TCM properties of warming and fortifying spleen. The correlation between Maka’s effective mechanism and enhancing mitochondrial energy metabolism enzymes is further confirmed.
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