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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    30 July 2018, Volume 41 Issue 7 Previous Issue    Next Issue
    Chinese medicine in new situation: inheritance and innovation*
    Wang Yongyan, Tian Jinzhou
    2018, 41 (7):  533-536.  doi: 10.3969/j.issn.1006-2157.2018.07.001
    Abstract ( 439 )   PDF (1640KB) ( 290 )   Save
    China’s average life expectancy is prolonged. Chronic diseases related to aging have become the focus of disease prevention and control. Effective inheritance and innovation of traditional Chinese medicine can enable it to be the leading way for chronic diseases’ prevention and treatment in the new era.The paper stressed characteristics of high concept-big data era should be paid attention to during formulation of preventive and therapeutic strategy. The original sources of ancient science and philosophy in prehistoric Hetu Luoshu (River Map and Luo Book) and Taiji diagram are interpreted at idea level and combined with philosophy and mathematics, thenphilosophic mathematics, an emerging inter-discipline, is induced. The holographic Taiji diagram with image-numerology combination is established after integrating the science and philosophy in Hetu Luoshu with yin and yang concepts, and circular motion diagram with correspondence between nature and human is established after integrating five phase theory. The philosophic thoughts returned image thinking, including “all things by means of the Dao”, “unitary concordance” and “to value unity and great harmony” are uncovered, innovation is emphasized on the base of inheritance, andtrain of thinking and approaches for preventing and treating chronic diseases including major brain disdords are expanded.
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    Connotation of heart desiring soft and application of supplementation-draining theory in Huangdi Neijing (Yellow Emperor’s Internal Classic)*
    Wang Ranran, HE Juan
    2018, 41 (7):  537-541.  doi: 10.3969/j.issn.1006-2157.2018.07.002
    Abstract ( 631 )   PDF (1191KB) ( 262 )   Save
    The theory of supplementation and draining according to desire of five viscera, from Suwen · Zangqi Fashi Lun (Plain Questions · On Visceral Qi and Seasons), is an important theoretical base for guiding visceral medication, in particular for desire of five viscera and medication laws. In desire of five viscera, the connotation of heart desiring soft is hard to understand compared with desire of other 4 viscera. Heart is characterized by fire and has physical characteristics of heating the superior and flaring up. But Suwen said heart desiring soft, and physicians of all dynasties had no clear comments on this, and later generations feel puzzled. In this paper, the connotation of heart desiring soft is explained from 3 aspects, heart controlling mental activities (characterized by soft and lenience), heart controlling blood vessels (characterized by soft and fluent) and heart opening into tongue (characterized by soft and flexible). The medication laws for heart desiring soft with salty supplementation and sweet draining are interpreted, and viewpoints are promoted, including salty medicinal can impair hyperactive heart yang, which is called supplementation and actually is draining, and sweet medicinal can inspire declined heart yang, which is called draining and actually is supplementation.
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    On pathogenesis of distal metastasis of moderate and advanced lung cancer based on theory of wind-toxin entering collaterals*
    He Wei
    2018, 41 (7):  542-546.  doi: 10.3969/j.issn.1006-2157.2018.07.003
    Abstract ( 402 )   PDF (1194KB) ( 164 )   Save
    The distal metastasis of moderate and advanced lung cancer, based on disease course, condition, nature, severity and morbidity, conforms to pathogenic wind’s features of rapid movement and swift changes and violent changeable characteristics of cancer toxin, which dues to internal wind carrying cancer toxin and transferring into wind toxin. Wind toxin is a pathogenic factor with disease-evil natures, symptomatic manifestations and pathogenesis characteristics of wind-evil and toxin. It can be classified into external wind toxin and internal wind toxin. The external wind toxin promotes the development of lung cancer, and internal wind toxin mainly takes part in the evolution of lung cancer pathogenesis, which is a turning point of lung cancer development. The wind toxin mainly takes collaterals and triple energizers as its route of metastasis and thrusts medullar collaterals, which causes bone and brain metastases of middle and advanced lung cancer. The principal pathogenesis is dual deficiency of lung qi and yin and disorders of governing management leading production of wind toxin. The initial pathogenesis of wind stirring inside of lung cancer is that hundred vessels become estranged, stasis toxin hinders collaterals and wind forms. The core pathogenesis of brain metastases of lung cancer is that liver body and function become unbalance, and wind toxin thrusts brain orifice and medullar collaterals. The principal pathogenesis of bone metastasis of lung cancer is that kidney essence is deficient, kidney’s functions of reproduction and growth are dysfunctional, and wind toxin flows bone cavity and medullar collaterals. Discussing the pathogenesis of bone and brain metastasis of lung cancer based on theory of wind-toxin entering collaterals has a higher clinical reference value for enriching the theoretical system of etiology and pathogenesis of TCM oncology, determining the visceral levels of tumor, confirming the key factors of tumor metastasis and improving the accuracy of tumor differentiation and treatment.
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    Influence of Jiawei Shaoyao Gancao Tang on proliferation of adenomyosis cells through regulating P53-273H*
    Jiang Xinchan, Li Kunyin, Guan Yongge, Wang Shuai, Guo Yudan
    2018, 41 (7):  547-552.  doi: 10.3969/j.issn.1006-2157.2018.07.004
    Abstract ( 362 )   PDF (1437KB) ( 139 )   Save
    Objective To study the influence of Jiawei Shaoyao Gancao Tang (Modified Debark Peony Root and Liquorice Root Decoction, JSGT) on proliferation of adenomyosis(AM) cells through regulating P53-273H. Methods The human AM cells were collected and cultured, and then divided randomly into JSGT group, mifepristone group and blank group. After treatment with corresponding medicinals for 24 h and 48 h, the influence of JSGT on apoptosis of AM focus cells was detected by using flow cytometry (FCM). The expressions of P53-273H mRNA and LIFR mRNA of AM cells were detected by using real-time reverse transcription quantitative polymerase chain reaction (RT-PCR). The protein expressions of P53-273H, LIF, STAT3 and p-STAT3 were detected by using Western blotting assay. Results The results of FCM showed that the apoptosis rate was significantly higher in JSGT group than that in blank group (P<0.01). The results of real-time RT-PCR showed that the relative expressions of P53-273H mRNA and LIFR mRNA were lower in JSGT group than those in blank group (P<0.05). The results of Western blotting assay showed that, after intervention with JSGT for 24 h and 48 h, the protein expressions of P53-273H, LIF, STAT3 and p-STAT3 were lower in JSGT group than those in mifepristone group (P<0.05) and blank group (P<0.05). Conclusion JSGT can inhibit the proliferation and improve the apoptosis of AM cells, and the mechanism may be related to that JSGT can regulate P53/LIF/STAT3 signal pathway.
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    Influence of Qishan Fang combined with cisplatin on PI3K/AKt signal pathway of lung cancer A549 cells*
    Sui Yutong, Chi Wencheng, Jiang Jiakang
    2018, 41 (7):  553-558.  doi: 10.3969/j.issn.1006-2157.2018.07.005
    Abstract ( 316 )   PDF (1253KB) ( 128 )   Save
    Objective To observe the influence of Qishan Fang (Milkvetch Root and Taxus Chinensis Decoction) combined with cisplatin on PI3K/AKt signal pathway of lung cancer A549 cells. Methods Male Wisatr rats were randomly divided into group of Qishan Fang medicated serum (serum group), cisplatin group, medicated serum combined with cisplatin group (combined group) and blank control group (control group, each n=10). The serum samples were collected after all groups were orally given corresponding medicinal for 3 d. The influence of Qishan Fang medicated serum combined with cisplatin on proliferation, apoptosis and relative protein and genetic expressions of A549 cells were detected respectively by using methyl thiazolyl tetrazolium test (MTT), Annexin V-PE/7-AAD double-staining, Western blotting assay and real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Results The inhibition of A549 cell proliferation was observed in serum group, cisplatin and combined group, and the inhibitory rate was, respectively 27.20% (P<0.01), 28.52% (P<0.01) and 51.25% (P<0.01). The protein and genetic expressions of Caspase-3, Caspase-9, mTOR and PI3K decreased in serum group, cisplatin and combined group compared with control group (P<0.01). The influence on protein expression related to A549 cell apoptosis was the most significant in combined group (P<0.01). Conclusion The proliferation of A549 cell can be inhibited, relative apoptosis pathways of Caspase-3, Caspase-9, AKT and mTOR can be activated in serum group, cisplatin group and combined group, and apoptosis of A549 cell can be induced. Qishan Fang combined with cisplatin has better regulative effect than single of them, and inhibitions of proliferation rate and apoptosis rate are significantly improved in combined group than those in cisplatin group and serum group.
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    Maka, its TCM properties and influence on mitochondrial energy metabolism enzymes in spleen deficiency mice*
    Fei Wenting, Hou Yan, Wang Yujie, Wang Zichen, Li Aimin, Wang Linyuan, Zhang Jianjun
    2018, 41 (7):  559-566.  doi: 10.3969/j.issn.1006-2157.2018.07.006
    Abstract ( 372 )   PDF (1185KB) ( 304 )   Save
    Objective To investigate the effects of relieving physical fatigue and anti-oxidative damage of Maka (Maca, Lepidium meyenii Walp.), to verify Maka’s properties of warming and fortifying spleen, and to discuss the mechanism of energy metabolism in mice with spleen deficiency pattern. Methods Male Kunming mice were randomly divided into normal group, model group, ginseng group (control group) and high-dose, mid-dose and low-dose Maka groups (1.5 g/kg, 0.75 g/kg, 0.25 g/kg). Except of normal group, other groups were given abnormal diet and fatigue to impair spleen for 14 d for establishing mouse model of spleen deficiency pattern. Meanwhile all groups were preventively given corresponding drugs at the first day. The time of forced swimming test (FST) was recorded in spleen deficiency mice. The activities or concentration of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidease (GSH-PX) and malondialdehyde (MDA) in liver tissue homogenate were detected by using chromatoptometry. The concentration of serum cAMP and cGMP were detected by using radioimmunoassay (RIA), and ratio of cAMP to cGMP (cAMP/cGMP) was calculated. The activity of F0F1-ATP enzyme was detected by using continuous cycle method, and activities of Na+-K+-ATP and Ca2+-Mg2+-ATP enzymes were detected by using enzymolysis. Results Compared with normal group, mouse body weight and time of FST decreased, activities of SOD, CAT and GSH-PX decreased, MDA content increased, cAMP/cGMP decreased, and activities of F0F1-ATP, Na+-K+-ATP and Ca2+-Mg2+-ATP decreased in model group (P<0.01). Compared with model group, mouse body weight was increased in all Maka groups (P<0.01). The time of FST was significantly prolonged in high-dose Maka group and ginseng group (P<0.01), which was not significant in mid-dose and low-dose Maka groups. The activities of SOD, CAT and GSH-PX were significantly increased (P<0.01), MDA content was decreased (P<0.01), cAMP/cGMP was significantly increased (P<0.01), and activities of F0F1-ATP, Na+-K+-ATP and Ca2+-Mg2+-ATP were significantly increased (P<0.01) in mid-dose and high-dose Maka groups, which was no significant in low-dose Maka group. Conclusion Maka, in a certain dose, can relieve physical fatigue, improve activities of anti-oxidative enzymes and eliminate cytotoxicity of free radicals, which verifies Maka’s TCM properties of warming and fortifying spleen. The correlation between Maka’s effective mechanism and enhancing mitochondrial energy metabolism enzymes is further confirmed.
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    Comparison in pharmacodynamics between modified paste and decoction of Jinhui Shenqi Pill in rat model of kidney yang deficiency*
    Zheng Longfei, Han Ling, Hao Yu, Gao Zhili, Shi Lingsheng, He Juan
    2018, 41 (7):  567-571.  doi: 10.3969/j.issn.1006-2157.2018.07.007
    Abstract ( 323 )   PDF (1148KB) ( 286 )   Save
    Objective To compare and study the curative effects of modified paste and decoction of Jinhui Shenqi (Kidney-qi Pill in Synopsis of the Golden Chamber) Pill in intervention of rat model of kidney yang deficiency. Methods Male SD rats (n=40) were divided into normal group, model group, group of Jinhui Shenqi Pill paste (paste group) and group of Jinhui Shenqi Pill decoction (decoction group). Expect of normal group, other groups were orally given adenine (200 mg/kg) for 4 weeks for copying rat model of kidney yang deficiency pattern. The paste group and decoction group were respectively given modified paste or decoction of Jinhui Shenqi Pill for 6 weeks. The model group was orally given distilled water and ginseng group was given conventional breeding. On the end of the 6th week, the serum samples were collected from all groups for detecting the hormones of hypothalamus-pituitary-gonadal axis including testosterone (T), estradiol (E2), luteinizing hormone (LH) and human chorionic gonadotropin (HCG), hormones of hypothalamus-pituitary-adrenal axis including adrenocorticotrophic hormone (ACTH), cortisol (COR), 17-hydroxycorticosteroid (17-OH-CS), and hormones of hypothalamus-pituitary-thyroid axis including tri-iodothyronine (T3) and tetra-iodothyronine (T4). Results The content of LH decreased significantly in model group compared with normal group (P<0.05), and increased significantly in paste group and decoction group (P<0.05) but there was no significant difference between paste group and decoction group. The content of E2 decreased significantly in model group compared with normal group (P<0.05), increased significantly in decoction group compared with model group (P<0.05). There was no significant difference in content of E2 between paste group and model group, but it was significantly higher in decoction group than that in paste group (P<0.05). Compared with normal group, content of T had descending trend without significant difference. The content of T increased significantly in paste group and decoction group compared with model group (P<0.05), while there was no significant difference between paste group and decoction group. The content of 17-OH-CS increased significantly in model group compared with normal group (P<0.05), decreased significantly in decoction group compared with model group (P<0.05), and decreased significantly in decoction group compared with paste group (P<0.05). The content of T4 decreased significantly model group compared with normal group (P<0.05), increased significantly in paste group compared with model group (P<0.05) and increased significantly in paste group compared with decoction group (P<0.05). Conclusion The two types of preparation forms of Jinhui Shenqi Pill (paste and decoction) can play a role of warm tonification of kidney yang through increasing content of LH and T, but there is difference between these two types of preparation forms. The decoction of Jinhui Shenqi Pill can warmly tonify kidney yang through increasing content of E2 and decreasing content of 17-OH-CS, but paste of Jinhui Shenqi Pill has no significant effect on these two indexes. The paste of Jinhui Shenqi Pill can warmly tonify kidney yang through increasing content of T4, while decoction of Jinhui Shenqi Pill has no significant effect on this index.
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    Influence of Dahuang Zhechong Pill on expression of BAMBI in primary hepatic stellate cells in rats*
    Xu Xinjie, Liu Xudong, Zhao Zhuangzhi, Lyu Ping
    2018, 41 (7):  572-578.  doi: 10.3969/j.issn.1006-2157.2018.07.008
    Abstract ( 309 )   PDF (2149KB) ( 147 )   Save
    Objective To investigate the effective mechanism of Dahuang Zhechong (Rhubarb and Dormant Insect) Pill in inhibition and reversion of hepatic fibrosis through detecting the expression of bone morphogenic protein and activin membrane-bound inhibitor (BAMBI) in primary hepatic stellate cells (HSC). Methods The model of hepatic fibrosis was established in clean Wistar rats, primary HSC in vivo were isolated and cultured, and then divided into normal group, model group and Dahuang Zhechong Pill group. After culturing for 24 h, the genetic expressions of TLR4, MyD88 and BAMBI in HSC were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). The protein expressions of HSC were detected by using immunohistochemistry technique and Western blotting assay, and activity of nuclear factor-κB (NF-κB) was detected by using electrophoretic mobility shift assay (EMSA). Results Compared with normal group, the protein and genetic expressions of TLR4 and MyD88 increased (P<0.001), and protein and genetic expressions of BAMBI decreased (P<0.001) in model group. Compared with model group, the protein and genetic expressions of TLR4 and MyD88 decreased (P<0.05), and protein and genetic expressions of BAMBI increased (P<0.05) in Dahuang Zhechong Pill group. The protein and genetic expressions of TLR4 and MyD88 were higher (P<0.05), and protein and genetic expression of BAMBI were lower (P<0.05) in Dahuang Zhechong Pill group than those in normal group. The results of EMSA showed that the activation of NF-κB was improved in model group compared with normal group, and was inhibited in Dahuang Zhechong Pill group compared with model group. Conclusion Dahuang Zhechong Pill can relieve hepatic fibrosis, and the effective mechanism may be related to reducing expressions of TLR4 and MyD88, inhibiting activation of NF-κB and improving expression of BAMBI in HSC, and reducing HSC sensitivity to TGF-β.
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    Changes of cAMP-PKA signal transduction pathways in small intestinal tissue between spleen qi deficiency pattern and kidney qi deficiency pattern in rats*
    Lyu Xiaoming, Song Nan, Wang Deshan, Shan Dehong, Yang Guanlin, Jia Lianqun
    2018, 41 (7):  579-584.  doi: 10.3969/j.issn.1006-2157.2018.07.009
    Abstract ( 310 )   PDF (1293KB) ( 261 )   Save
    Objective To compare the changes of cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signal transduction pathways in ileum tissue between spleen qi deficiency pattern and kidney qi deficiency pattern in rats. Methods Male SD rats (n=24) were randomly divided into normal group, spleen qi deficiency group and kidney qi deficiency group. The model of spleen qi deficiency pattern was established by repletion for 1 d, fasting for 2 d and swimming for 15 d, and model of kidney qi deficiency pattern, by intraperitoneal injection of kanamycin (250 mg/kg) twice a day for 14 d. Taking food and drinking water were freely for all rats. The activity of cAMP in ileum tissue was detected by using ELISA in all groups after modeling. The mRNA and protein expressions of PKA, glycogen phosphorylase (GP) and phosphorylase kinase (PHK) were detected by using RT-PCR and Western blotting assay. Results The content of cAMP decreased significantly in spleen qi deficiency group and kidney qi deficiency group compared with normal group (P<0.05 or P<0.01), and decreased more in spleen qi deficiency group (P<0.01). The mRNA and protein expressions of PKA decreased significantly in spleen qi deficiency group (P<0.01) and protein expressions of PKA decreased in kidney qi deficiency group (P<0.05) compared with normal group. Compared with normal group, the mRNA and protein expressions of PHK decreased significantly in spleen qi deficiency group (P<0.01) and protein expressions of PHK decreased in kidney qi deficiency group (P<0.05). The mRNA expression of PHK was higher in kidney qi deficiency group than that in spleen qi deficiency group (P<0.05). The mRNA and protein expressions of GP decreased significantly in spleen qi deficiency group (P<0.01), and mRNA expression of GP decreased in kidney qi deficiency group (P<0.01) compared with normal group. The mRNA and protein expressions of GP were higher in kidney qi deficiency group than those in spleen qi deficiency group (P<0.01). Conclusion The changes of cAMP-PKA signal transduction pathways have difference between spleen qi deficiency pattern and kidney qi deficiency pattern in rats. As to cAMP content, it decreases more in spleen qi deficiency group compared with kidney qi deficiency group, it can be deduced that low energy performances (lack of strength, etc.) are more serious in spleen qi deficiency pattern.
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    Simultaneous determination of 7 coumarin constituents in Lyophilized Powder of Zhiqiao Houpo Tang by using HPLC-MS/MS*
    Xu Jianjun, Ren Ping, Liu Yalin, Yan Han, Chen Ken, Shi Shaoqi, Huang Xi
    2018, 41 (7):  585-592.  doi: 10.3969/j.issn.1006-2157.2018.07.010
    Abstract ( 335 )   PDF (2068KB) ( 204 )   Save
    Objective To establish a method for quantitative determination of 7 coumarin constituents, including isoimperatorin, bergapten, xanthotoxol, auraptene, 6′,7′- dihydroxybergamottin, bergamottin, and bergaptol in Lyophilized Powder of Zhiqiao Houpo Tang (Lyophilized Powder of Fructus-aurantii Magnolia-bark Decoction, LPFM) by using high performance liquid chromatography-mass spectrometry-tandem mass spectrometry (HPLC-MS/MS). Methods HPLC-MS/MS procedure was performed on the chromatographic column of Waters BEH C18 (100 mm×2.1 mm, 1.7 μm) at 35 ℃. The mobile phase was acetonitrile (A)-0.1% formic acid water (B) in gradient elution, flow velocity was 0.25 mL/min and sample size was 10 μL. The mass spectrometry was operated with an electrospray ion (ESI) source for detecting positive ion mode. The mode of multi-reaction monitoring (MRM) was applied for quantitative determination. Results In 7 coumarin constituents, isoimperatorin showed a good linear relationship at a range from 1.01 μg/L to 101.2 μg/L (r=0.999 6), and average recovery was 98.42% (RSD=1.88%). Bergapten showed a good linear relationship at a range from 5.25 μg/L to 525.0 μg/L (r=0.999 8), and average recovery was 99.92% (RSD=1.30%). Xanthotoxol showed a good linear relationship at a range from 5.06 μg/L to 506.4 ng/mL (r=0.999 6), and average recovery was 100.40% (RSD=1.76%). Auraptene showed a good linear relationship at a range from 4.96 μg/L to 496.0 μg/L (r=0.999 7), and average recovery was 98.65% (RSD=1.80%). 6′,7′-Dihydroxybergamottin showed a good linear relationship at a range from 4.98 μg/L to 497.8 μg/L (r=0.999 9), and average recovery was 101.16% (RSD=1.36%). Bergamottin showed a good linear relationship at a range from 0.99 μg/L to 98.6 μg/L (r=0.999 6), and average recovery was 102.05% (RSD=1.65%). Bergaptol showed a good linear relationship at a range from 5.05 μg/L to 505.0 μg/L (r=0.999 8), and average recovery was 100.73% (RSD=1.71%). Conclusion The method is quick, sensitive and accurate, which can be used for the quantitative determination of 7 coumarin constituents, including isoimperatorin, bergapten, xanthotoxol, auraptene, 6′,7′-dihydroxybergamottin, bergamottin, and bergaptol in LPFM.
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    Simultaneous determination of 6 components in serum from Mongolian medicine compound formula-Shudage-4 by using HPLC*
    Xin Shasha, Ji Zengyun, Du Yinfei, Dong Yu
    2018, 41 (7):  593-598.  doi: 10.3969/j.issn.1006-2157.2018.07.011
    Abstract ( 360 )   PDF (1542KB) ( 167 )   Save
    Objective To establish a method for simultaneously determining the content of 1-phenyl-7-(3-methoxy-4-hydroxy) phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3- methylether, α-asarone and costunolide in serum from Mongolian medicine compound formula-Shudage-4 by using HPLC. Methods The HPLC procedure was performed on the chromatographic column of Agilent C18 (4.6 mm×250 mm, 5 μm) at 30℃, and mobile phase was acetonitrile (A)-0.2% water solution of acetic acid (B) in gradient elution. The flow velocity was 0.6 mL/min, sample size was 100 μL and detection wavelength was 254 nm. Results The mass concentration and peak areas of 1-phenyl-7-(3-methoxy-4-hydroxy) phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3-methylether, α-asarone and costunolide showed good linear relationships (r=0.999 0-0.999 8). The average recoveries of samples (n=6) were 91.74%- 102.10%, and RSD were 1.03%-2.61%. The average content of 1-phenyl-7-(3-methoxy-4- hydroxy) phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3-methylether, α-asarone and costunolide was, respectively, 3.200 0 mg/L, 0.647 5 mg/L, 0.865 6 mg/L, 0.156 0 mg/L, 0.331 9 mg/L and 3.075 1 mg/L. Conclusion The method is easy and stable with higher repeatability, and it can be used in quality control of Shudage-4 and provide a basis for serum pharmacochemistry study of Shudage-4 resources.
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    Influence of Huwang Mingmu Tang on hemodynamics and serum ICAM-1 and ET-1 changes in patients with diabetic retinopathy*
    Chen Mei, Cai Rang, Qi Huiyan, Wang Yongqin, Hu Zuquan
    2018, 41 (7):  599-604.  doi: 10.3969/j.issn.1006-2157.2018.07.012
    Abstract ( 308 )   PDF (1160KB) ( 181 )   Save
    Objective To investigate the improving effects of Huwang Mingmu Tang (Protecting Retina and Improving Eyesight Decoction) combined with calcium dobesilate on local hemodynamics, serum inflammatory factors and vascular endothelial factors in patients with diabetic retinopathy (DR). Methods DR patients (n=80) were chosen and divided, according to random digital table, into treatment group and control group (each n=40, 80 eyes). The control group was orally given calcium dobesilate capsules 1 capsule/time and 3 times/d. The treatment group was additionally given Huwang Mingmu Tang 1 dose/d, 2 times/d and 10 d/course for 3 courses. The changes of local hemodynamics, serum inflammatory factors and vascular endothelial factors were observed in 2 groups before and after treatment. Results The levels of peak systolic velocity (PSV) and end-diastolic velocity (EDV) of central retinal artery (CRA), posterior ciliary artery (PCA) and ophthalmic artery (OA) increased, and vascular resistance index (RI) decreased in 2 groups after treatment (P<0.01). The levels of PSV and EDV of CRA, PCA and OA were all higher (P<0.01), and RI was lower (P<0.01) in treatment group than those in control group after treatment. The levels of tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 (ICAM-1) and interleukin-6 (IL-6) were lower in 2 groups after treatment than before (P<0.01), which was more significant in treatment group (P<0.01). The levels of vascular endothelial growth factor (VEGF) and endothelin-1 (ET-1) decreased in 2 groups after treatment than before (P<0.01), which was more significant in treatment group (P<0.01). Conclusion Huwang Mingmu Tang combined with calcium dobesilate can treat DR effectively through changing hemodynamics and reducing levels of serum inflammatory factors and VEGF, and has good application prospect in clinic.
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    A randomized controlled trial on smoking cessation by acupuncture*
    Wang Yingying, Liu Chao, Wu Yuan, Tong Shuai, Su Li, Yuan Guihong, Jing Jumei, Zhang Lu, Yang Jinsheng
    2018, 41 (7):  605-610.  doi: 10.3969/j.issn.1006-2157.2018.07.013
    Abstract ( 369 )   PDF (1150KB) ( 239 )   Save
    Objective To review smoking cessation by acupuncture and its curative effect on abstinence symptoms. Methods The volunteers trying to quit smoking were divided, according to central randomization (1∶1), into acupuncture group and nicotine patch group (patch group), and a randomized controlled trial was applied. The acupuncture group was given quit-smoking intervention by needling in Baihui (GV20), Lieque (LU7), Hegu (LI4), Zushanli (ST36), Sanyinjiao (SP6) and Taichong (LR3), and acupuncture manipulation was of neutral supplementation and draining. Meanwhile electro-acupuncture apparatus was used to stimulate Lieque (LU7) and Zushanli (ST36) with continuous wave (15 Hz) for 30 min for 3 times a week. The patch group was given quit-smoking intervention with nicotine patches. All groups were intervened for 8 weeks and followed up for 16 weeks. The difference in abstinence rate based on expiratory CO content at 24-h time point was compared between 2 groups. The score changes of Fagerstrom Test for Nicotine Dependence (FTND), heaviness of smoking index (HSI), Minnesota Nicotine Withdrawal Scale (MNWS) and Brief Questionnaire of Smoking Urges (QSU-Brief) were compared by using intentional statistical analysis. Results There were totally 200 cases chosen, and at the end of follow-up, there were 30 missed in acupuncture group and 27 in patch group. There were no adverse events observed in 2 groups. After intervention for 8 weeks, the abstinence rate based on expiratory CO content at 24-h time point was 36.00% in acupuncture group and 46.00% in patch group (P>0.05). During 24-week follow-up period, the abstinence rate was 43.00% in acupuncture group and 44.00% in patch group (P>0.05). After intervention for 8 weeks, the difference in FTND, HIS, MNWS and QSU-Brief had no statistical significance between 2 groups (P>0.05). During 24-week follow-up period, the difference in FTND, HIS, MNWS and QSU-Brief had no statistical significance between 2 groups (P>0.05). Conclusion Acupuncture is a safe and effective intervening measure for smoking cessation, and can effectively relieve abstinence symptoms.
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    Blood flow changes in sinew bind point in healthy people and focus of bound sinew in patients with knee osteoarthritis observed based on laser speckle imaging*
    Zhang Xiaoqing, Dong Baoqiang, Lin Xingxing, Zhang Zhixing, Mai Ke
    2018, 41 (7):  611-616.  doi: 10.3969/j.issn.1006-2157.2018.07.014
    Abstract ( 301 )   PDF (1215KB) ( 162 )   Save
    Objective To observe the difference in blood flow (BF) between physiologic sinew bind point and pathologic focus of bound sinew by using laser speckle imaging (LSI) in patients with knee osteoarthritis (KOA). Methods The patients with KOA of gallbladder meridian type diagnosed by pattern differentiation of meridian sinew (n=100) were chosen into KOA group, and healthy volunteers (n=100) were chosen into health group. The average BF [10 frames were counted and mean value was calculated (PU)] was observed, recorded and compared after needling distal lingxiaci of the same meridian sinew in 5 sinew bind points (focuses of bound sinew) including linghouci, yanglingci, feiguxiaotou, chengfeijian and chengguci before, during and after needling (5 min, 10 min, 15 min, 20 min) by using moor FLPI-2 system. Results Before needling, BF was higher in linghouci, yanglingci, feiguxiaotou, chengfeijian and chengguci in KOA group than that in health group (P<0.001). During needling, BF was higher in linghouci, yanglingci, feiguxiaotou, chengfeijian and chengguci in KOA group than that in health group (P<0.001). After needling, BF was higher in linghouci, yanglingci, feiguxiaotou, chengfeijian and chengguci in KOA group than that in health group at all time points (P<0.05, P<0.01, P<0.001). Conclusion BF shows a significant ascending feature of LSI in focuses of bound sinew compared with sinew bind points, which may be related to that focuses of bound sinew can reflect disease conditions.
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