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主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

Table of Content

    30 June 2018, Volume 41 Issue 6 Previous Issue    Next Issue
    Analyzing the origin and evolution of theories and text styles of HuangdiNeijing*
    Shi Lingsheng, He Juan
    2018, 41 (6):  445-450.  doi: 10.3969/j.issn.1006-2157.2018.06.001
    Abstract ( 404 )   PDF (1263KB) ( 352 )   Save
    Huangdi Neijing(Huangdi’s Internal Classic) is a medical classic in its own system in early ancient China. Through exploring the shared rules of ancient literatures inheritance, this study investigated the origin and evolution of theories and text styles of Huangdi Neijing. The theories of Huangdi Neijing derived from the medical experience of senior official doctor in Zhou Dynasty. In a long course of history, Huangdi Neijing was handed down between generations from ear to ear, mouth to mouth, until the particular written history set in.In the early period, the text was written on the bamboo slips and was distributed chapter by chapter. In Han Dynasty, these scatted chapters were collected and compiled into a medical book. In Tang Dynasty, Wang Bing, a TCM practitioner, reorganized the chapters and finalized the version, which is still available in modern days.
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    The functional symptoms of Jin in TCM*
    Xu Yang, Zhang Jianxiong, Zhang Qiming, Wang Quannian, Wang Yiguo, Lyu Jian, Shi Yingjie
    2018, 41 (6):  451-454.  doi: 10.3969/j.issn.1006-2157.2018.06.002
    Abstract ( 400 )   PDF (1441KB) ( 311 )   Save
    From textbooks for TCM universities and colleges, 30 TCM foundational symptoms of Jin (sinew) were selected in accordance with the TCM understanding of “liver storing blood and nourishing sinew”, and symptoms, physiology, and pathology of external wind patterns. 30 symptoms includes dazzle, blindness, ophthalmic nystagmus, tinnitus, epicophosis, frequent blink, upward staring eyes, single eyelid ptosis, tongue tremor, tongue stiffness, tongue crook, lockjaw, neck stiffness, shoulder shrugging and head jerking, facial paralysis, frequent wink, numbness, vertigo, gait abnormality(sensational ataxic gait), vestibular nystagmus, limb tremor, limb convulsion, limb spasm, muscular twitching, limb wriggling, opisthotonos, flaccid paralysis, spastic paralysis, limb stiffness, and hemiplegia. Corresponding to Jin in TCM, the biomedical concept may refer to visual, auditory, equilibrium, superficial sensory, deep sensory signal pathways, and the motion signal pathways dominating skeletal muscle movement. The specific function of Jin are to transmit the visible, audible, equilibratory sensation, superficial sensation, deep sensory signals, and the motion signals dominating skeletal muscle movement.
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    On relationship among skipping pulse, bound pulse, intermittent pulse and arrhythmia*
    Shen Wuxia, Wang Tianfang, Huo Yanming, Chen Xinran
    2018, 41 (6):  455-458.  doi: 10.3969/j.issn.1006-2157.2018.06.003
    Abstract ( 437 )   PDF (1102KB) ( 866 )   Save
    Skipping pulse, bound pulse and intermittent pulse are intermittent pulse conditions commonly seen in clinic, and they are closely related to arrhythmia in Western medicine. They are different pulses but they are generally referred as “intermittent pulses” without any distinguishment in clinical practice. This paper did an inductive analysis on ancient and modern literature about skipping pulse, bound pulse, intermittent pulse and arrhythmia. Skipping pulse is an irregular intermittent pulse with rapid pulse rate, and it can be seen in fast atrial fibrillation, irregular premature contractions, tachycardia and atrial flutter. Bound pulse is also an irregular intermittent pulse but with slower pulse rate, and it can be seen in sinus arrest, sinoatrial conduction block, atrioventricular block, irregular premature systole and escape beat. Intermittent pulse is a regular intermittent pulse with normal, rapid or slow pulse rate, and it can be seen in regular premature contraction, sinoatrial conduction block and atrioventricular block.
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    Improving contemporary compilation methods of TCM classics from the perspective of national cultural heritage systemization
    Gu Jianjun,Li Chengwen
    2018, 41 (6):  459-463.  doi: 10.3969/j.issn.1006-2157.2018.06.004
    Abstract ( 352 )   PDF (1105KB) ( 330 )   Save
    The core of classic compilation is to explore and find the academic value. For TCM classic compilation, national cultural heritage systemization plays a key role in transforming from traditional methods to contemporary methods. The Objective of TCM classics compilation is to serve the clinical practice. According to national cultural heritage systemization, the academic, ideological values, and rules of medical development need to be explored in TCM classic compilation. Different TCM schools compiled TCM classics using a variety of categories including by material medica, formulas, cases, principles, diseases, and thinking patterns, which demonstrate the academic values of TCM classics, and could be considered as useful attempts in TCM classic compilation.
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    Effects of three-step sequential therapy on the concentrations of relevant parameters in TGF-β1/Smad signal pathway among asthma rats during steroid withdrawal*
    Tian Yan, Cui Hongsheng, Qin Yang, Li Qiannan, Qin Fangfang, Ren Ying, Zhang Shujing, Liu Baoshan
    2018, 41 (6):  464-470.  doi: 10.3969/j.issn.1006-2157.2018.06.005
    Abstract ( 309 )   PDF (1148KB) ( 167 )   Save
    Objective To observe the influence of three-step sequential therapy on the TGF-β1/Smad signal pathway in rats with asthma during steroid withdrawal. Methods One hundred and fifty male SD rats were randomly divided into blank control group, airway remodeling group, hexadecadrol group, pulmicortrespules group and three-step sequential treatment group. The rat models were established by using sensitization of ovalbu-min (OVA) (100mg) and aluminum hydroxide (100mg). The models were stimulated by using 1% OVA, and from the third week, hexadecadrol (0.5mg/kg) was injected intraperitoneally on the first 30min before stimulation for 2 consecutive weeks. The dosage of hexadecadrol was reduced at a rate of 0.1mg/kg per week, and it was stopped on the 7th week. Rats in the three-step sequential therapy group received extra gavage administration of the first-stage formula (yin-nourishing, heat-clearing,phlegm-resolving and qi-downbearing) on the 15th to 28thday, followed by second-step formula(yin-yang regulating & tonifying,blood-activating and phlegm-resolving)on the 29th to 63rd day, and the third-step formula(yang-warming and blood-harmonizing, qi-regulating)on the 64thto 77th day. The alveolar lavage fluid of rats were obtained on the 28th, 63rd, and 77th day after 2 hours of the last stimulation, and the relevant parameters of TGF-β1/Smadsignal pathway were measured by using enzyme linked immunosorbent assay. Results In the process of steroid withdrawal, compared with the blank control group, the concentration of TGF-β1, Smad2 of rats in hexadecadrol group and airway remodeling group were higher (P<0.05); Smad3 were significantly higher (P<0.01) and Smad7 were lower (P<0.05). Compared with hexadecadrol group,the concentration of TGF-β1 , Smad2 and Smad3 of rats in the three-step sequential therapy group decreased (P<0.05) and the concentration of Smad7 increased (P<0.05). Conclusion Three-step sequential therapy could inhibit airway remodeling of rats in the process of steroid withdrawal, possibly though regulating the TGF-β1/Smadsignal pathway.
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    Effects of modified biejiajian pill on the proliferation and apoptosis of hepatocellular carcinoma HCCLM3 cells*
    Gao Yebo, Li Weidong, Liu Rui, Guo Qiujun, Jiang Shulong
    2018, 41 (6):  471-475.  doi: 10.3969/j.issn.1006-2157.2018.06.006
    Abstract ( 390 )   PDF (1482KB) ( 178 )   Save
    Objective To investigate the effects of modified biejiajian(Turtle Shell Decoction, BJJ) pill on the proliferation and apoptosis of hepatocellular carcinoma HCCLM3 cells. Methods Modified BJJ pill was orally administered to SD rats at 21 g/kg dosage for 7 days. Blood samples were drawn from the rat abdominal aorta at d7 and then centrifuged to prepare medicated serum. Modified BJJ medicated serum at different concentrations were added into 96-well-plates. At 24 h, 48 h, 72 h, 10 μL MTT solution was further added into each well, incubated for another 4h, and effects of medicated serum on the proliferation of HCCLM3 were then observed; Cultured HCCLM3 cells were administered with Chinese medicine(CM) medicated serum (5%,10%,20%) and cucurbitacin I, then the cells were incubated for 24h and 48h, on which Annexin V-FITC/PI was used to evaluate the effects of medicated serum on HCCLM3 apoptosis. Results At 24h after treated with BJJ at different concentrations, 5% and 10% of BJJ had little effect on HCCLM3 cell proliferation, and the difference was not statistically significant compared with the control group (P>0.05). After 48-h treatment of BJJ, cell proliferation rates were significantly higher in the BJJ and BJJ groups (87.86%, 84.60% respectively) compared with the control group (P<0.05). 5% BJJ had little effect on HCCLM3 cell apoptosis, and the difference was not statistically significant (P>0.05) compared with control group. Apoptosis rates were 7.90%, 12.22% and 15.36% in 10% BJJ, 20% BJJ and cucurbitacin I (0.5μmol/l) groups respectively with significant difference compared with the control group (P<0.05). After 48-h treatment, apoptosis rates in the 5%,10%,and 20% BJJ, and cucurbitacin I (0.5μmol/l) groups were 8.08%, 11.95%, 13.89% and 19.02% respectively, statistically higher than that in the control group (P<0.05). Conclusion s Modified BJJ medicated serum could inhibit the proliferation and increase the apoptosis rates of HCCLM3 cells. The effects were dose-dependent.
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    Effects of curcumin on the expression of microRNA-21 and fibrosis-related factors in rat renal interstitial fibroblast cells*
    Liu Yi, Wang Xiting, Zhang Lan, Sun Xuejiao, Han Sen, Tang Lei, Li Yadong, Li Yu
    2018, 41 (6):  476-481.  doi: 10.3969/j.issn.1006-2157.2018.06.007
    Abstract ( 333 )   PDF (1824KB) ( 190 )   Save
    Objective To investigate the effects of curcumin on expression of microRNA-21 and fibrosis-related factors induced by transforming growth factor-beta 1(TGF-β1) in the renal interstitial fibroblast cells (NRK49F) of rats. Methods NRK49F cells were divided into normal group, model group, and curcumin groups. NRK49F cells in the model group were activated by TGF-β1(10μg/L). Cells in the curcumin groups were treated with curcumin at high, medium, and low concentration(20,10,5μmol/L respectively) for 24 and 72 hours. The protein expression of α-SMA were measured by using immunofluorescence cell staining and cell analyzer. The expression of micro RNA-21 and mRNA of fibrosis-related factors (COL1A1, COL1A2, COL3A1, ACTA2, Smad3) were measured with RT-PCR. Results After treated with TGF-β1 for 72 hours, the protein expression level of α-SMA increased significantly(P<0.01);Compared with TGF-β1-treated group, the protein expression level of α-SMA decreased significantly in the curcumin groups (20,10,5 μmol/L)(P<0.01, P<0.01, P<0.05). After treated with TGF-β1 for 24 hours, the expression level of miR-21, COL1A1, COL3A1, α-SMA, and Smad3 mRNA increased significantly (P<0.01). Compared with TGF-β1-treated group, the expression level of miR-21, COL1a1, COL3a1, α-SMA, and Smad3 mRNA decreased significantly in the curcumin groups (20,10 μmol/L)(P<0.01, P<0.05);Compared with TGF-β1-treated group, the expression level of COL1A1, COL3A1, and α-SMA mRNA decreased significantly (P<0.01) and the expression level of miR-21 and smad3 mRNA showed a downward trend in the 5 μmol/L curcumin group. Conclusion Curcumin down-regulated fibrosis-related gene and protein expression induced by TGF-β1 in rat renal interstitial fibroblasts, which may be related to its down-regulating the expression of miR-21 genes.
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    Effect of Yiqi Huoxue Granule on urine metabolomics in rats with myocardial infarction*
    Wang Hui, Zheng Chenglong, Guo Shuwen, Li Fanghe, Huang Xiaolou, Tan Xiaobo, Cai Qian, Wu Jiani, Zhang Yuqin, Lin Wang’ou, Zhang Binyue
    2018, 41 (6):  482-489.  doi: 10.3969/j.issn.1006-2157.2018.06.008
    Abstract ( 316 )   PDF (2264KB) ( 208 )   Save
    Objective To observe the effect of Yiqi Huoxue(qi-boosting and blood-activating,YQHX)Granule on urinary metabolites in rats with myocardial infarction. Methods Myocardial infarction (MI) model was established by ligation of left anterior descending cornary artery. Rats with successful modeling were randomly divided into 7 d group and 28 d group. In both groups rats were further randomly divided into MI model group, perindopril group, Qishen Yiqi(astragalus root and ginseng qi-boosting,QSYQ) pill group and YQHX granule group, and a sham operation group with puncture but no ligation was set up.The corresponding medication was given on the second day after operation. Rats' urine was collected after 7th and 28th of medication.The urine samples contained the same types of metabolites, but the concentrations were different. The metabolites in the 7-day and 28-day groups were screened with nuclear magnetic resonance and the differences in sham operation group and model group were compared. Based on the shared metabolites, the differences between the HQHX granule group and the model group were compared. Results After gavage for 7 days, 16 kinds of differential metabolites were identified. In the model group, 9 different metabolites were found in the urine compared with the sham group (P<0.05). The model group included lysine, β-hydroxybutyrate, valine, glutamic acid, and oxaloacetate. The concentration was lower than that of the sham group, but the concentrations of asparagine, fructose, isoleucine, and serine were higher than those of the sham group. The YQHX granule group was compared with the model group, six kinds of differential metabolites were found (P<0.05). The concentrations of lysine, β-hydroxybutyrate, glutamic acid, and oxaloacetate in the YQHX granule group were higher than those in the model group. In the group, the concentration of fructose and isoleucine was lower than that of the model group. After 28 days of gavage, 25 kinds of differential metabolites were initially screened. In the model group, 14 different metabolites were found in the urine compared with the sham group (P<0.05), including β-hydroxybutyrate, glutamic acid, methylamine, cysteine, betaine, and choline in the model group. The concentrations of lactate, lactic acid, proline, threonine, malic acid and nicotinic acid were lower than those in the sham operation group, but the concentrations of ornithine, γ-aminobutyric acid and α-glucose were higher than those in the model group. 12 kinds of differential metabolites were found in the YQHX granule group compared with the model group (P<0.05). YQHX granule group glutamic acid, cysteine, betaine, choline, lactic acid, valine, threonine The concentration of malic acid, nicotinic acid, and niacin were higher than those in the MI model group. The concentrations of ornithine, methylamine, and α-glucose were lower than those in the model group. Conclusion There are multiple metabolic disorders in rat after MI, and YQHX granules can effectively regulate the disorder metabolites and promote its return to normal range.
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    Effects of Yi’nao Jieyu formula on magnetic resonance spectroscopy of rats with post-stroke anxiety*
    Zhang Yuan, Zhao Ruizhen, Zhao Zijun, Tang Qisheng
    2018, 41 (6):  490-496.  doi: 10.3969/j.issn.1006-2157.2018.06.009
    Abstract ( 357 )   PDF (1522KB) ( 178 )   Save
    Objective To observe metabolism changes in rats with post-stroke anxiety (PSA) and effects of Yi’nao Jieyu(brain-benefiting depression-resolving, YNJY)formula by using magnetic resonance spectroscopy. Methods Wistar rats were randomly divided into normal group, model group, sham-operation group, and YNJY group. PSA model was established by injecting collagenase (type VII) into the right globus pallidus combined with empty water bottle stimulation. Saline was injected instead of collagenase in rats of the sham-operation group. Rats in the YNJY group received YNJY formula (4.19 g/kg) for 4 weeks afterwards while those in the normal, model, and sham-operation groups were given distilled water (10 mL/g). Morphology and behavior (elevated plus maze) were observed after 4 weeks. Magnetic resonance spectroscopy was utilized at week 4 as well. Magnetic resonance spectroscopy imaging was the last one to be detected. Bilateral prefrontal cortex and hippocampus were scanned in each rat and the relative contents of N-acetylaspartic acid (NAA), choline-containing compounds (Cho), myoinositol(mI), glutamate (Glu) and creatine (Cr) were recorded. Results 1. Morphology: Compared with the normal group, rats in the model group ate less, hadmessy and dull fur, behaved aggressively, and gnawed the cage frequently. Compared with the model group, rats in the YNJY group had less aggressive behaviors, and their fur appeared softer and smoother. 2. Elevated plus maze: entry into and duration in open arms were statistically significantly less in the model group than the normal and YNJY group(P<0.05). 3. Magnetic resonance spectroscopy: model rats had lower level of NAA/Cr and Cho/Cr, and higher level of Glu/Cr in the right pre-frontal cortex than normal rats (P<0.05, P<0.01), but there was no significant differences in left pre-frontal cortex and both hippocampi (P>0.05). Compared with the model group, levels of NAA/Cr and Cho/Cr were higher in the YNJY group, while Glu/Cr level was lower in the right pre-frontal cortex (P<0.05). Conclusion YNJY formula could restore cerebral metabolism caused by PSA, relieve the post-stroke anxiety, the mechanism of which may be related with reducing amino acid neurotransmitters and repairing of impaired neurons.
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    Effects of shaoyang-dredging method on HBV-induced protein expression of pSmad3 in HK-2 Cells*
    Xu Bing, Wang Yaoguang, Zhou Huijie
    2018, 41 (6):  497-501.  doi: 10.3969/j.issn.1006-2157.2018.06.010
    Abstract ( 323 )   PDF (1291KB) ( 224 )   Save
    Objective To observe the effects of shaoyang (lesser yang)-dredging method on HBV-induced transdifferentiation and pSmad3’s protein expression in HK-2 cells, with the attempt to explore the mechanism of shaoyang-dredging method in treating HBV associated glomerulonephritis(HBV-GN). Methods MTT assay was used to measure the concentration of chinese medicine(SSN), a representative formula of dredging lesser yang. 9 concentration gradients were set. Absorbance was measured at the OD570nm of a microplate reader and the cell survival rate was calculated. Half inhibitory concentration (IC50) was estimated according to the drawn growth curve, and this IC50 concentration would be used in the next steps. HK-2 cells were treated with pHY106-HBV agent and TGF-β1 stimulation. HK-2 cell lines were divided into 6 groups: blank control group(routine culture of HK-2 cells); empty plasnid group(HK-2 cells transfected with empty plasmid pHY106); HBV group(HK-2 cells transfected with pHY106-HBV); TGF-β1 group(HK-2 cells with 12 ng of recombinant human TGF-β1); HBV+SSN group(HK-2 cells treated with Chinese medicine 6h after being transfected by pHY106-HBV); and TGF-β1+SSN group(HK-2 cells treated with chinese medicine 6 h after being stimulated by recombinant human TGF-β1).48 hours later, pSmad3 expression was evaluated with Western blot assay. Results Based on the cell growth curve plotted according to the cell survival rate, the IC50 value calculated was equivalent to 1/200-diluted 1.313 g/ml crude drug concentration. The expression of pSmad3 protein increased in HBV group compared with empty plasmid group, and the difference was statistically significant (P<0.05). The expression of pSmad3 protein was also significantly increased in TGF-β1 group compared with blank control group(P<0.05). Compared with HBV group, the expression of pSmad3 was markedly reduced in SSN group(P<0.05). This reduction was also observed in TGF-β1 group reduced compared with TGF-β1 group(P<0.05). Conclusion At 1/200 of crude drug concentration 1.313 g/ml, the representative formula of dredging lesser yang method can reduce the pSmad3 protein expression. It is further speculated that it can affect the transdifferentiation of HK-2 cells by inhibiting Smad-mediated signal transduction pathways.
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    Simultaneous determining three active components of qi-boosting and pulse-restoring granule with HPLC*
    Zhang Ziwei, Wang Min, Tan Jiawei,Cui Yahua, Zhang Qiang, Jia Shan, Du Shouying, Bai Jie, Ma Junming, Wan Jie
    2018, 41 (6):  502-507.  doi: 10.3969/j.issn.1006-2157.2018.06.011
    Abstract ( 384 )   PDF (1636KB) ( 273 )   Save
    Objective To establish a method for simultaneous determination of paeoniflorin, salvianolic acid B and tanshinoneⅡA in yiqi fumai keli (qi-boosting and pulse-restoring, YQFM) granule. Methods The contents of paeoniflorin, salvianolic acid B and tanshinoneⅡA were determined by using HPLC method as Hypersil Gold Aq RPC18 column (4.6mm × 250mm, 5 μm) was utilized; the mobile phase was gradient-eluted by acetonitrile-0.05%phosphoric acid. The flow rate was 1.3 mL/min. As for the detection wave length, 230nm was set for Paeoniflorin,286 nm for salvianolic acid B and 270 nm for tanshinoneⅡA.The column temperature was 30℃and the sample injection’s volume was 10 μL. Results Paeoniflorin showed a good linear relationship with the peak at the range of 24.36~507.42 mg/L and the rvalue was 0.999 1. Salvianolic acid B showed a good linear relationship with the peak at the range of 17.40 to 362.48 mg/L and the rvalue was 0.999 8. TanshinoneⅡA showed a good linear relationship with the peak area at the range of 0.97 to 20.18 mg/L andthe r value was 0.999 9. The average recovery rates of the three components were all between 95% and 105% and the RSD values were all less than 3.00%. Conclusion The method seems accurate,specific, sensitive, and can be used for the quality control of qi-boosting and pulse-restoring granule.
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    Time-variant effects of needling Zhigou on the rest-state fractional amplitude of low-frequency fluctuations inside healthy human brains*
    Han Hongyan, Wang Keke, Wang Wenxian , Zhang Chi, Hu Yicong, Yan Bin
    2018, 41 (6):  508-515.  doi: 10.3969/j.issn.1006-2157.2018.06.012
    Abstract ( 358 )   PDF (3675KB) ( 247 )   Save
    Objective To observe time-variant effects of needling Zhigou (SJ6) on fractional Amplitude of Low Frequency Fluctuations(fALFF) in thebrainsof healthy subjects atresting state by using functional magnetic imaging (fMRI). Methods Eighteen healthy participants were scanned with fMRI. The scanning involved pre-treatment pure resting state(R1), then needle-retention resting states(R2-R5) and post-treatment resting states (R6~R7). All gathered data was analyzed with fALFF method. Results ①The fALFF of bilateral precuneus transformed from inactivation before treatment topositive activation after treatment and maintained tillR6 state(except for R5), and the amplitude as well as clusters of activation both remained stable. ②fALFF of superior frontal gyrus and medial frontal gyrus were strengthened on the basis of pre- treatment activation within R1; alternating positive activation on both sides of the two gyri were observed within R2, R4, R5 and R6 states except for R3 state, during within which both sides had positive activations. ③Compared with R1, the fALFF of inferior parietal lobule transformed from deactivations to positive activations only within R2 and R3 state, as there were no significant changes within R4-R7.④The fALFF of cerebellum posterior lobe presented positive activation within R2 state and both cerebellum anterior and posterior lobe presented positive activation within R6 while made deactivations within R3 and R5 states. Conclusion s The findings of this study could provide certain theoretical foundation for acupuncture on SJ6 in the treatment of diseases with weakened function of precuneus, superior frontal gyrus, medial frontal gyrus, inferior parietal lobule and strengthened function of cerebellum anterior and posterior lobefrom the perspective of brain energy metabolism. It could also provide some scientific evidence for the timing of manipulating needle during needle retention and studies of post-acupuncture effects.
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    Effects of acupuncture combined with herbal medicine on the transplant outcome of frozen-thawed embryos and receptivity of endometria in kidney-deficiency patients with multiple implantation failure*
    Yang Yuan, Li Dong, Xin Xiyan, Luo Bin
    2018, 41 (6):  516-522.  doi: 10.3969/j.issn.1006-2157.2018.06.013
    Abstract ( 424 )   PDF (1139KB) ( 267 )   Save
    Objective To observe the effects of acupuncture combined with herbal treatment on the transplant outcome of frozen-thawed embryos and receptivity of endometria in kidney-deficiency patients with multiple implantation failure. Methods 120 patients who met the diagnostic criteria were randomly divided into a treatment group and a control group. Patients in the control group were treated with routine natural cycle freeze-thaw embryo transfer protocol. Apart from routine protocol, patients in the treatment group took blood activating formula every day and received acupuncture on guanyuan (RN4), qihai (RN6), zigong (EX-CA1), sanyinjiao (SP6, bilateral), taichong (LR3, bilateral), hegu (LI4, bilateral) every other day before the menstruation. No herbal treatment nor acupuncture was administered during the menstruation. After the menstruation, the patients resumed herbal treatment (blood-activating kidney-tonifying formula) every day and acupuncture treatment (RN4, RN6, EX-CA1, bilateral SP6, ST36, and KI3) every other day. Treatment course was three menstrual cycles. Outcome measurements were syndrome scores of kidney deficiency before and after the treatment, estradiol (E2) and progesterone (P) levels, endometrial thickness and blood flow distribution during implantation window. Biochemical and chemical pregnancy rates were also calculated in the two groups after frozen-thawed embryo transfer. Results Symptoms of kidney deficiency had been significantly relievedin the treatment group (P<0.05); this improvement was better than that of the control group (P<0.05). The post-treatment progesterone (P) and estradiol (E2) levels in the corpus luteum phase in the treatment group were significantly higher than those in the confrol group before and after the treatment (P<0.05). The endometrial blood flow distribution is divided into type A, type B and type C. After the treatment, the proportion of type A’s and type B’s distribution of endometrial blood flow were significantly increased, and the proportion of type C was significantly decreased (P<0.05). The post-treatment ratio of type A, B and C in the treatment group were compared between the two groups and there was no statistical difference in type A; but the proportion of type B was significantly higher than that of the control group (P<0.05), and the proportion of type C was significantly lower than that of the control group (P<0.05). The post-treatment endometrial thickness in the treatment group increased remarkedly (P<0.05). After the treatment, the endometrial thicknesses of the treatment group were significantly higher than that of the control groups (P<0.05). After the treatment, the biochemical pregnancy rate and clinical pregnancy rate of the treatment group were higher than those of the control group, and the differences were statistically significant (P<0.05). Conclusion Acupuncture combined with herbal therapy can significantly improve the patient's kidney-deficiency symptems; it can also regulate estrogen and progesterone levels and benefit embryonic implantation, improve endometrial thickness and endometrial blood flow, thereby enhancing endometrial tolerance and improve clinical pregnancy rate.
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    Effects of Kudiezi injection on serum neurotransmitters in patients with acute cerebral infarction*
    Zeng Zixiu, Liu Xuemei, Liang Xiao, Chen Baoxin, Liu Xiaohan, Wen Binyu, Xu Zhenmin, Fang Xiaolei, Zhang Yunling
    2018, 41 (6):  523-528.  doi: 10.3969/j.issn.1006-2157.2018.06.014
    Abstract ( 352 )   PDF (1200KB) ( 161 )   Save
    Objective To observe changes of serum neurotransmitters in patients with acute cerebral infarction on the 1st, 3rd, 5th and 14th days after admission, and to study the therapeutic effects of the Kudiezi(KDZ) injection. Methods Forty patients with acute cerebral infarction within 48 hours were recruited and randomly divided into routine treatment group (n=20), routine treatment+KDZ injection group (n=20). Patient serum samples were collected on the1st, 3rd, 5th, and 14thdays to measure concentration of glutamate (Glu) and 5-hydroxytryptamine (5-HT) by using UPLC-Q-TOF-MS. Results Kudiezi injection could significantly decrease the concentration of 5-HT and Glu over time. Concentrations of Glu at day 5 and 14 and 5-HT in KDZ treatment group at day 3 and 14 were significantly lower than those in the routine treatment group at the same time (P<0.05);and after 14 days of treatment, the concentration of Glu decreased significantly in the KDZ treatment group(P<0.05). Conclusion Kudiezi injection can significantly reduce the serum concentrations of Glu and 5-HT in acute cerebral infarction patientsat early time.
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