Effect of Yiqi Huoxue Granule on urine metabolomics in rats with myocardial infarction*
2018, 41 (6):
Objective To observe the effect of Yiqi Huoxue(qi-boosting and blood-activating,YQHX)Granule on urinary metabolites in rats with myocardial infarction. Methods Myocardial infarction (MI) model was established by ligation of left anterior descending cornary artery. Rats with successful modeling were randomly divided into 7 d group and 28 d group. In both groups rats were further randomly divided into MI model group, perindopril group, Qishen Yiqi(astragalus root and ginseng qi-boosting,QSYQ) pill group and YQHX granule group, and a sham operation group with puncture but no ligation was set up.The corresponding medication was given on the second day after operation. Rats' urine was collected after 7th and 28th of medication.The urine samples contained the same types of metabolites, but the concentrations were different. The metabolites in the 7-day and 28-day groups were screened with nuclear magnetic resonance and the differences in sham operation group and model group were compared. Based on the shared metabolites, the differences between the HQHX granule group and the model group were compared. Results After gavage for 7 days, 16 kinds of differential metabolites were identified. In the model group, 9 different metabolites were found in the urine compared with the sham group (P<0.05). The model group included lysine, β-hydroxybutyrate, valine, glutamic acid, and oxaloacetate. The concentration was lower than that of the sham group, but the concentrations of asparagine, fructose, isoleucine, and serine were higher than those of the sham group. The YQHX granule group was compared with the model group, six kinds of differential metabolites were found (P<0.05). The concentrations of lysine, β-hydroxybutyrate, glutamic acid, and oxaloacetate in the YQHX granule group were higher than those in the model group. In the group, the concentration of fructose and isoleucine was lower than that of the model group. After 28 days of gavage, 25 kinds of differential metabolites were initially screened. In the model group, 14 different metabolites were found in the urine compared with the sham group (P<0.05), including β-hydroxybutyrate, glutamic acid, methylamine, cysteine, betaine, and choline in the model group. The concentrations of lactate, lactic acid, proline, threonine, malic acid and nicotinic acid were lower than those in the sham operation group, but the concentrations of ornithine, γ-aminobutyric acid and α-glucose were higher than those in the model group. 12 kinds of differential metabolites were found in the YQHX granule group compared with the model group (P<0.05). YQHX granule group glutamic acid, cysteine, betaine, choline, lactic acid, valine, threonine The concentration of malic acid, nicotinic acid, and niacin were higher than those in the MI model group. The concentrations of ornithine, methylamine, and α-glucose were lower than those in the model group. Conclusion There are multiple metabolic disorders in rat after MI, and YQHX granules can effectively regulate the disorder metabolites and promote its return to normal range.
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