Analyzing chemical composition of Mongolian compound Xieriga-4 in vivo and in vitro*
2018, 41 (10):
Objective To establish the in vitro and in vivo HPLC profiles of Mongolian drug compound Xieriga-4, and assign and identify its chemical composition in vitro and in vivo. Methods To take SD rats and intragastrically administer a certain amount of Mongolian medicine compound Xieriga-4 to prepare in vivo serum and metabolic urine, and measure with HPLC. comparing the effective components in drug-containing serum and urine, an in vitro and in vivo HPLC profile of Mongolian drug compound Xieriga-4 was established. Results The in vitro HPLC profiles of Mongolian medicine compound Xieriga-4 were analyzed, and a total of 53 peaks were found. There were 35 blood components after in vivo administration of Mongolian Medicine Compound Xieriga-4, and 25 blood components were directly injected into the blood. The other 10 were considered new metabolites. Peaks 7, 9, 12, 14, 15, 17, 26, 27, 30, 33, 51, 53 originated from turmeric, Peaks 18 and 19 came from Cork; the peaks 1, 2, 3, 10, and 16 from lice; the peaks 8, 18, 31, and 32 from earthworms. Four incoming chemical constituents were identified through in vitro and in vivo chemical composition comparisons. Three of them were identical to those in metabolism and it was assumed that they may act as prototype components and be excreted. Conclusion Blood components and metabolites may be effective components of compound effects. This article speculates that the composition of blood components of the compound into the blood to speculate the mechanism of its treatment, in order to provide further evidence to explore the mechanism of Mongolian medicine compound Xieriga-4 treatment of diseases.
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