Effects of Dendrobium huoshanense on fatigue and energy metabolism in mice with kidney-yin deficiency*
2018, 41 (12):
Objective To explore the effects of Huoshan Shihu (Dendrobium huoshanense, HSSH) on fatigue and energy metabolism regulation in mice with kidney-yin deficiency.Methods Mice model with kidney-yin deficiency was established by gavage of thyroxine. Effects of Huoshan Shihu on mice with kidney-yin deficiency were observed. The thermotropism behavior and the weight-loaded swimming time of mice were monitored. The levels of T3 and T4 were detected by using Enzyme-linked Immunosorbent Assay (ELISA); cAMP, cGMP, LDH and CK in serum were detected with radioimmunoassay, cAMP/cGMP was thns calculated. and the Na+-K+-ATP-ase and Ca2+-Mg2+-ATP-ase in liver tissue of mice were detected with chromatometry. Results Compared with the normal animals, the time duration in high-temperature zone and the loaded-swimming time of mice in model group were significantly decreased (P＜0.01). The levels of T3, T4, cAMP, and cAMP/cGMP were significantly increased(P＜0.01,P＜0.001). The activities of LDH were decreased(P＜0.05), and CK, Na+-K+-ATP-ase and Ca2+-Mg2+-ATP-ase were increased (P＜0.001). Compared with the model animals, time duration in high-temperature zone and loaded-swimming time in A (0.33 g/kg), B (1 g/kg), C (2 g/kg), and D (3 g/kg) dose of HSSH group, Tiepi Shihu (Dendrobium candidum, TPSH) group, and Liuwei Dihuang Wan (Six-Ingredient Rehmannia pill, LWDH) group increased significantly; the level of cAMP/cGMP decreased significantly (P＜0.05, P＜0.01); and the activities of Na+-K+-ATP-ase and Ca2+-Mg2+-ATP-ase decreased significantly (P＜0.01,P＜0.001). B, C, and D dose of HSSH group, TPSH group and LWDH group appeared to reduce the concentration of T3 and T4 (P＜0.05, P＜0.01, P＜0.001). the activities of LDH were significantly increased in B and C dose of HSSH, TPSH and LWDH groups, and the levels of CK decreased (P＜0.05, P＜0.01, P＜0.001). Conclusion With the effects of nourishing yin and clearing heat, Huoshan Shihu could regulate the energy metabolism rate, improve the loaded-swimming time, and reduce the activity of energy metabolizing enzymes and the thyroxine level of the mice with kidney-yin deficiency induced by thyroxine. Its anti-fatigue mechanism may be related to the regulation of energy metabolism enzymes. This study further proves that the property of Huoshan Shihu is cold.
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