Determination of phenolic parts in ThymusquinquecostatusCelak by HPLC and UV-Vis*
2019, 42 (10):
ObjectiveTo establish methods for the determination of active components from phenolic parts of folk medicine Thymus quinquecostatus Celak by using HPLC and UV-Vis. Methods For HPLC, scutellarin and rosmarinic acid were used as references. The mobile phase A was acetonitrile while mobile B was 0.2% formic acid. The flow rate was set at 1.0 mL/min and the column temperature was maintained at 25 ℃ . 10 μL sample solutions were injected into auto sampler and monitored at 280 nm. For UV-Vis, developer dosage of K3[Fe(CN)6]-FeCl3 were chosen, rosmarinic acid was used as reference, the dosage of reagent was 2 mL, the determination time was 60 min and the detection wavelength was 760 nm. Results The linear ranges of scutellarin and rosmarinic acid were 0.019~0.592 g/L (R2=0.999), 0.999~0.280 g/L (R2=1.000), respectively. The RSDs(n=6) of precision experiment were 0.21%, 0.56%, respectively. The RSDs(n=6) of stability experiment were 0.21%, 0.56%, respectitvely. The RSDs(n=6) of reproducibility experiment were 1.35%, 1.47%, respectively, while the average adding standard recoveries were 99.42%, 95.31%, 104.49% (RSD=1.48%, 1.46%, 0.35%) and 101.23%, 102.02%, 97.62%(RSD=0.29%, 1.38%, 2.05%). The linear ranges of total polyphenolsisl 1.10~2.05 mg/L (R2=0.999). The RSD(n=6) of precision experiment was 0.97%. The RSD(n=6) of stability experiment was 1.32%. The RSD(n=6) of reproducibility experiment was 0.89%, while the average adding standard recoveries was 100.88%(RSD=1.55%). The average content of scutellarin and rosmarinic acid in all three batchesphenolic parts was 8.40% and 8.23%, respectively, while the averagecontent of total polyphenols was 47.40%.
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Conclusion Both methods were simple, accurate, specific and sensitive, and can be foundations for the development and subsequent research of T. quinquecostatus Celak.