Effect of medicated serum of NaotaifangⅡ on microglia polarization induced by LPS*
2020, 43 (5):
Objective To investigate the influence of medicated serum of Naotaifang Ⅱ on the morphology, M1/M2 polarization markers and inflammatory factors of HAPI cells induced by LPS. Methods The inflammation model of HAPI cell was induced by LPS. The cultured cells were divided into: blank group, model group, Naotaifang Ⅱ group, and minocycline group. Morphology and quantity of microglia were observed by High Content Imaging System. mRNA expression of M1-type markers (MCH II, iNOS, MCP-1, CD11b) and M2-type markers (Arg1, Mrc1, Ym1), pro-inflammatory factors (IL-1β, IL-6, IL-12, TNF-α) and anti-inflammatory factors (IL-4, IL-10, TNF-β) was detected by qRT-PCR. Results Compared with the blank group, in the model group, microglia cell body became larger while showing multipolarization or amoeba type with coarser and shorter synapse; the mRNA level of M1 markers and pro-inflammatory increased significantly (P< 0.01); moreover, the expression of M2 marker Mrc1 and anti-inflammatory factors IL-4 and TNF-β decreased dramatically (P<0.01). Compared with the model group, in NaotaifangⅡgroup, the cell swelling recovered, the synapses became longer, and the microglia increased. Besides, in NaotaifangⅡgroup, the mRNA expression of M1 markers MHCⅡ, iNOS and CD11b and the pro-inflammatory factors were significantly decreased (P<0.01), and the mRNA expression of M2 markers and anti-inflammatory factors were significantly increased (P<0.01). Compared with the minocycline group, the Naotaifang Ⅱ group showed no significant advantage in reducing the expression of M1 markers (P>0.05), while better effect on lowering the expression of pro-inflammatory factors IL-6, IL-12 and TNF-α (P<0.01) except IL-β. Besides, NaotaifangⅡcould better increase mRNA expression of M2 markers Mrc1, Ym1 and anti-inflammatory factors IL-4, TGF-β compared with the minocycline group. Conclusion Naotaifang seems to inhibit M1 polarization, promote M2 polarization by regulating cell morphology, M1/M2 markers and inflammatory factors. It could possibly enhance M2 markers and anti-inflammatory factors better than minocycline.
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