Preventive effects of Yiqi Jiedu Fang on acute testicular injury of mice induced by 2.0 Gy 60Co γ ray*
2020, 43 (9):
Objective To observe the acute testicular injury in male Balb/c mice induced by 2.0 Gy 60Co γ rays and the preventive effect of Yiqi Jiedu Fang (Qi-boosting and Toxin-removing Decoction, YQJDF) administered before irradiation. Methods 200 male Balb/c mice were randomly divided into five groups: normal group, model group, positive drug group, and high- and low-dose YQJDF groups. Except for the normal group, the other mice were subjected to single-dose whole-body irradiation with 2.0 Gy 60Co γ rays. The mice in the intervention groups were given their corresponding drugs for 10 consecutive days before the irradiation, and at the same time the mice in the normal group and the model group were given deionized water. Mice tissues were collected 1 day before and on the 1st, 3rd, and 7th days after the irradiation, and the body weight, testicular parameters, morphological changes of the testis, and apoptosis of spermatogenic cells were measured. Results Compared with the normal group, there was no significant difference in the above mentioned parameters of mice between groups 1 day before the irradiation. However, 1 day after irradiation, mice in the model group had a decreased Johnsen score of testis and an increased apoptosis rate of spermatogenic cells. On the 3rd day after irradiation, the weight of mice in the model group and the positive drug group decreased, the Johnsen score of mice in the model group, the positive drug group and the high-dose YQJDF group decreased, and the apoptosis rate of spermatogenic cells in all groups subjected to irradiation increased. On the 7th day after irradiation, testicular parameters decreased in all the irradiated groups, testicular morphology and structure changed, Johnsen score decreased significantly, and the spermatogenic cell apoptosis rate increased further. Compared with the model group, on the 3rd and 7th day after the irradiation, the Johnsen score of all the intervention groups were notably higher, and the apoptosis rate of spermatogenic cells were significantly lower, and the testicular pathological changes were lighter. Conclusion 2.0 Gy 60Co γ ray can cause morphological and structural changes in mouse testis, and increase the apoptosis of spermatogenic cells, and in turn affect the spermatogenic function of mice. The preventive treatment with YQJDF before irradiation effectively reduced the above injuries.
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