Effects of Tongluo Zhitong Ningjiao on synovial membrane inflammation severity of KOA rabbit model at different stages*
2020, 43 (12):
Objective To observe the effects of Tongluo Zhitong Ningjiao (Collateral-unblocking and Pain-relieving Gel, TLZTNJ) on the synovial membrane inflammation severity in rabbit models of knee osteoarthritis (KOA) at different stages, and explore its mechanism in the treatment of KOA. Methods 56 rabbits were randomly divided into 8 groups: normal group, sham operation group, mild KOA group, moderate KOA group, severe KOA group, mild KOA group treated with TLZTNJ, moderate KOA group treated with TLZTNJ, and severe KOA group treated with TLZTNJ. Except for the normal group and the sham operation group, KOA models in the other groups were established with the Hulth method. The mild KOA group, the moderate KOA group, and the severe KOA group were executed in the 4th, , 8th, and 16th, week respectively after the models were established. The mild, moderate, and severe KOA groups treated with TLZTNJ were treated with the gel for 3 weeks after the mild, moderate, and severe KOA groups were executed and were executed too. The degree of synovial inflammation was observed by HE staining. The levels of IL-1β, IL-6, TNF-α and PGE2 in serum were detected by ELISA. The mRNA and protein expressions of IL-1β, TNF-α and MMP-13 in synovial tissue were detected by qPCR and WB. Results The synovial layer of rabbits in the mild, moderate, and severe KOA groups significantly thickened, and the inflammatory infiltration increased. After the intervention with TLZTNJ, the severity of synovial inflammation in the mild, moderate, and severe KOA rabbit groups treated with TLZTNJ were significantly reduced. TLZTNJ lowered the levels of IL-1β, IL-6, TNF-α and PGE2 in the serum (P<0.05, P<0.01) and inhibited the expression levels of IL-1β, TNF-α and MMP-13 (P<0.05, P<0.01) of the rabbits with mild, moderate, and severe KOA. Conclusion TLZTNJ can significantly inhibit synovial membrane inflammation and reduce the expression of inflammatory factors in rabbit KOA models at different stages.
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