سابِق|تال

Study on the mechanism of Suxiao Jiuxin Pills in treating stable angina pectoris with qi stagnation and blood stasis based on metabonomics

  • role： First author第一作者
  • Affiliation：

    Department of Cardiovascular Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China

  • Introduction：原梦飞，男，在读博士生
YUAN Mengfei1,  
  • role： Corresponding author通信作者
  • Affiliation：

    Department of Cardiovascular Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China

  • Email：shenxiaoxupaper@sina.com
  • Introduction：Prof.SHEN Xiaoxu, Ph.D., Chief Physician, Doctoral Supervisor. Dongzhimen Hospital, Beijing University of Chinese Medicine, No.5, Haiyuncang, Dongcheng District, Beijing 100700. E-mail: shenxiaoxupaper@sina.com
SHEN Xiaoxu1*,  
  • Affiliation：

    Department of Cardiovascular Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China

WENG Jieqiong1,  
  • Affiliation：

    Department of Cardiovascular Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China

ZHANG Jingfang1,  
  • Affiliation：

    Department of Cardiovascular Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China

SHEN Xingchen1,  
  • Affiliation：

    Department of Cardiovascular Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China

WENG Yayi1

ملخص

ObjectiveTo identify changes in metabolites in patients with stable angina pectoris(SAP) with qi stagnation and blood stasis, explore the differential metabolites and metabolic pathways associated with SAP, and determine the therapeutic mechanisms associated with Suxiao Jiuxin Pills.MethodsPatients and healthy recruited from September 2019 to September 2021 in Dongzhimen Hospital and other departments. The experimental group included 30 SAP patients with qi stagnation and blood stasis, and the control group included 30 healthy individuals. Serum metabolomics analysis was performed on both groups using liquid chromatography/mass spectrometry (LC/MS), and the VIP values of the first two principal components of the multivariate partial least squares-discriminant analysis model combined with a univariate analysis of difference fold-change and P values were used to screen differentially expressed metabolites. Metabolic pathway enrichment analysis of differential metabolites was performed using the Kyoto Encyclopedia of Genes and Genomes database.ResultsA total of 18 significant differential metabolites were identified in the experimental and control groups; the levels of 4-hydroxycinnamimide, biliverdin-Ⅸ-β, and 2-methylene glutamic acid were lower in SAP patients than in healthy individuals, and the L-carnitine, vitamin BT, agmatine, citraconic acid, guanine, and menthol levels were higher. Twenty-one significant differential metabolites were identified in the experimental group before and after the administration of Suxiao Jiuxin Pills. The levels of bacterial chlorophyll-a, sphingosine, 4-oxoretinol, ceramide, 1-methylguanine, and other metabolites decreased after administration, while those of 6-ketoprostaglandin E1, resin toxin, and prostaglandin G2 increased. The abnormal changes in differential metabolites suggest that numerous metabolic pathways are disordered in SAP patients, including amino acids, lipids, retinol, purines, porphyrins, chlorophyll, inflammatory mediator regulation of transient receptor potential channels, and serotonergic synapses.ConclusionThere are significant differences in metabolites between patients with SAP with qi stagnation and blood stasis and healthy people, and Suxiao Jiuxin Pills have positive and negative effects on regulating serum metabolites in vivo, thus regulating intestinal flora, maintaining the normal intestinal barrier, improving blood lipid levels, increasing the stability of vascular endothelial function, reducing tissue cell inflammation response, and inducing antioxidant effects.

مفهوم

Suxiao Jiuxin Pills;stable angina pectoris;qi stagnation and blood stasis;metabolomics;liquid chromatography/mass spectrometry (LC/MS)

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