Früher|Nächster

Effect of Shenqi Jieyu Formula on magnetic resonance spectroscopy of Papez circuit-related brain regions in adult offspring of maternal separation rats

  • role： First author第一作者
  • Affiliation：

    Beijing University of Chinese Medicine, Beijing 100029, China

    Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China

  • Introduction：李伯梁，男，在读硕士生
LI Boliang12,  
  • Affiliation：

    Staff Hospital of China Power Construction Group Shandong Electric Power Construction First Engineering Co., Ltd., Jinan 250100, China

JIANG Shengnan3,  
  • Affiliation：

    Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China

TANG Qisheng2,  
  • Affiliation：

    Beijing University of Chinese Medicine, Beijing 100029, China

    Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China

SUN Di12,  
  • role： Corresponding author通信作者
  • Affiliation：

    Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China

  • Email：ivy_miao@163.com
  • Introduction：QU Miao, Ph. D., Associate Chief Physician, Master′s Supervisor. Beijing University of Chinese Medicine Third Affiliated Hospital, No. 51, Xiaoguan Street, Wai Andingmen, Chaoyang District, Beijing 100029. E-mail: ivy_miao@163.com
QU Miao2*

Resümee

ObjectiveTo explore the effect and mechanism of Shenqi Jieyu Formula (SQJYF) on depression and anxiety-like behavior in adult offspring rats after maternal separation (MS). This was done by observing the levels of metabolites in the hippocampus and prefrontal cortex of the Papez circuit through magnetic resonance spectroscopy (MRS).MethodsTwenty-four Sprague-Dawley rats at 16 days of pregnancy were divided into the normal, model, SQJYF, and fluoxetine groups using the random number table method, with six rats per group. MS was performed 4 h a day from the first to the 21st day after birth. Starting from the 15th day, the corresponding medications(SQJYF group: 12.5 g/kg, fluxetine group: 2.33 mg/kg) were administered to mother rats once a day for 7 consecutive days. Normal breastfeeding was performed during the gavage. The offspring rats were weaned on the 22nd day. According to the experimental grouping of mother rats, one male and one female offspring rat of each mother rat were randomly selected using the random number table method, with six rats per group. They were divided into normal, model, SQJYF, and fluoxetine female and male rat groups, respectively. After 8 weeks of feeding, the offspring rats in each group were subjected to forced swimming test(FST), the sucrose water consumption test, and open field tests (OFT), and the elevated plus maze (EPM) test was conducted. The relative values of N-acetyl-aspartate (NAA), choline (Cho), glutamic acid (Glu), myo-inositol (mI), and creatine (Cr) in the hippocampus and prefrontal cortex were detected using MRS.ResultsCompared with that of the normal female and male rat groups, the weight of the rats in the model group was decreased as well as sucrose water consumption and the horizontal and vertical scores of OFT, whereas the immobility time of FST increased. Compared with the normal female and male rat group, the NAA/Cr and Glu/Cr values in the bilateral hippocampus and prefrontal cortex of the female and male rats in the model group decreased (all P<0.01). The proportion of rats entering the open arm and open arm residence time of the model female rat group in the EPM test decreased, whereas the Cho/Cr value of the right hippocampus of the female rats in the model group increased (P<0.05). Compared with the model female and male rat group, the weight of the rats in the SQJYF and fluoxetine groups increased, the immobility time of FST decreased, but the sucrose water consumption and the horizontal and vertical OFT scores increased (P<0.01). The proportion of rats entering the open arm and open arm residence time in the EPM increased in the SQJYF and fluoxetine female rat groups compared to those of the model female rat group (P<0.01). Compared with the model female rat group, the NAA/Cr and Glu/Cr values in the bilateral hippocampus and prefrontal cortex of female rats in the SQJYF and fluoxetine groups increased. The NAA/Cr and Glu/Cr values in the right hippocampus and prefrontal cortex of male rats in the SQJYF and fluoxetine groups were higher than those in the model group (P<0.05), whereas the Cho/Cr value in the right hippocampus of female and male rats in the SQJYF and fluoxetine groups decreased (P<0.05).ConclusionMS in early life can lead to depression and anxiety-like behavior in adult offspring. However, SQJYF may exert antidepressant and anti-anxiety effects by regulating the metabolite levels in related brain regions of the Papez circuit.

Schlüsselwort

maternal separation;magnetic resonance spectroscopy;Papez circuit;offspring;Shenqi Jieyu Formula;rats

References

  1. [1].
    ROST F, BOOKER T, GONSARD A, et al. The complexity of treatment-resistant depression: a data-driven approach[J]. J Affect Disord, 2024, 358: 292-301.
  2. [2].
    YIN WW, PAN YL, ZHOU LH, et al. The relationship between childhood trauma and depressive symptom among Zhuang adolescents: Mediating and moderating effects of cognitive emotion regulation strategies[J]. Front Psychiatry, 2022, 13: 994065.
  3. [3].
    YACAMAN-MENDEZ D, HALLGREN M, FORSELL Y. Childhood adversities, negative life events and outcomes of non-pharmacological treatments for depression in primary care: a secondary analysis of a randomized controlled trial[J]. J Psychiatr Res, 2019, 110: 152-158.
  4. [4].
    LI YC, RONG J, ZHONG HQ, et al. Prenatal stress leads to the altered maturation of corticostriatal synaptic plasticity and related behavioral impairments through epigenetic modifications of dopamine D2 receptor in mice[J]. Mol Neurobiol, 2021, 58(1): 317-328.
  5. [5].
    WILLIAMS LM, DEBATTISTA C, DUCHEMIN AM, et al. Childhood trauma predicts antidepressant response in adults with major depression: data from the randomized international study to predict optimized treatment for depression[J]. Transl Psychiatry, 2016, 6(5): e799.
  6. [6].
    姜圣楠，曲淼，王丹，等. 母婴分离产后抑郁大鼠脑内5-羟色胺及其代谢产物的变化及参芪解郁方的干预作用[J]. 中华中医药杂志，2023, 38(2): 836-840.
  7. [7].
    张玉荣，王瑞忠，王莉，等. 母婴分离对子代大鼠海马Wnt信号分子的影响[J]. 第三军医大学学报，2021, 43(20): 2167-2172.
  8. [8].
    MINSHALL BL, SKIPPER RA, RIDDLE CA, et al. Sex differences in acute early life stress-enhanced fear learning in adult rats[J]. Dev Psychobiol, 2024, 66(5): e22511.
  9. [9].
    王岳青，曲淼，茹尘，等. 参芪解郁方对母婴分离产后抑郁大鼠磁共振波谱的干预作用研究[J]. 湖南中医药大学学报，2021, 41(3): 338-344.
  10. [10].
    曹云燕，蔡圣朝，崔倩倩，等. 行气调神针刺疗法对缺血性卒中后抑郁模型大鼠行为学影响的研究[J]. 山西中医学院学报，2013, 14(4): 4-8.
  11. [11].
    DE SOUZA JA, DA SILVA MC, COSTA FCO, et al. Early life stress induced by maternal separation during lactation alters the eating behavior and serotonin system in middle-aged rat female offspring[J]. Pharmacol Biochem Behav, 2020, 192: 172908.
  12. [12].
    吴如燕，陆彩，夏宝妹，等. 产后抑郁母代对子代小鼠青少年期抑郁发病的影响[J]. 中国医学科学院学报，2016, 38(2): 187-191.
  13. [13].
    SCARBOROUGH J, IACHIZZI M, SCHALBETTER SM, et al. Prenatal and postnatal influences on behavioral development in a mouse model of preconceptional stress[J]. Neurobiol Stress, 2024, 29: 100614.
  14. [14].
    LI MX, WU F, CAO Y, et al. Abnormal white matter integrity in Papez circuit in first-episode medication-naive adults with anxious depression: a combined voxel-based analysis and region of interest study[J]. J Affect Disord, 2023, 324: 489-495.
  15. [15].
    ZOU RY, TIEMEIER H, VAN DER ENDE J, et al. Exposure to maternal depressive symptoms in fetal life or childhood and offspring brain development: a population-based imaging study[J]. 2019, 176(9): 702-710.
  16. [16].
    PEÑA CJ, KRONMAN HG, WALKER DM, et al. Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2[J]. Science, 2017, 356(6343): 1185-1188.
  17. [17].
    JIANG ZJ, ZHU ZM, ZHAO MY, et al. H3K9me2 regulation of BDNF expression in the hippocampus and medial prefrontal cortex is involved in the depressive-like phenotype induced by maternal separation in male rats[J]. Psychopharmacology, 2021, 238(10): 2801-2813.
  18. [18].
    TAYLOR MJ, GODLEWSKA BR, NORBURY R, et al. Early increase in marker of neuronal integrity with antidepressant treatment of major depression: 1H-magnetic resonance spectroscopy of N-acetyl-aspartate[J]. Int J Neuropsychopharmacol, 2012, 15(10): 1541-1546.
  19. [19].
    YOON SJ, LYOO IK, HAWS C, et al. Decreased glutamate/glutamine levels may mediate cytidine′s efficacy in treating bipolar depression: a longitudinal proton magnetic resonance spectroscopy study[J]. Neuropsychopharmacology, 2009, 34(7): 1810-1818.
  20. [20].
    ROSENBERG DR, MACMASTER FP, MIRZA Y, et al. Reduced anterior cingulate glutamate in pediatric major depression: a magnetic resonance spectroscopy study[J]. Biol Psychiatry, 2005, 58(9): 700-704.
  21. [21].
    HONG ST, CHOI CB, PARK C, et al. Specific hippocampal choline decrease in an animal model of depression[J]. Br J Radiol, 2009, 82(979): 549-553.
  22. [22].
    LV SH, ZHONG SM, ZHANG SH, et al. Correlations between facial emotion processing and biochemical abnormalities in untreated adolescent patients with major depressive disorder: a proton magnetic resonance spectroscopy study[J]. J Affect Disord, 2022, 296: 408-417.
  23. [23].
    URRILA AS, HAKKARAINEN A, CASTANEDA A, et al. Frontal cortex myo-inositol is associated with sleep and depression in adolescents: a proton magnetic resonance spectroscopy study[J]. Neuropsychobiology, 2017, 75(1): 21-31.
  24. [24].
    VAESSEN T, REININGHAUS U, VAN AUBEL E, et al. Neural correlates of daily-life affective stress reactivity in early psychosis: a study combining functional MRI and experience sampling methodology[J]. Schizophr Res, 2023, 255: 93-101.
  25. [25].
    曲淼，孙文军，唐启盛. “枢轴运动”理论与产后抑郁症辨治[J]. 北京中医药大学学报，2021, 44(7): 631-634.
  26. [26].
    王丹，李小黎，赵瑞珍，等. 参芪解郁方对产后抑郁症患者雌、孕激素及EPDS评分的影响[J]. 北京中医药，2016, 35(1): 29-32.
  27. [27].
    杨歆科，李小黎，赵瑞珍，等. 产后抑郁大鼠脑前额叶皮层、海马区DA及其代谢产物、受体动态变化及参芪解郁方的干预作用[J]. 中华中医药杂志，2018, 33(8): 3304-3309.
  28. [28].
    曲淼，李净娅，唐启盛，等. Treg/Th17平衡对产后抑郁大鼠的影响及参芪解郁方的细胞免疫调节作用[J]. 中华中医药杂志，2015, 30(5): 1452-1458.
  29. [29].
    QU M, TANG QS, LI XL, et al. Shen-Qi-Jie-Yu-Fang has antidepressant effects in a rodent model of postpartum depression by regulating the immune organs and subsets of T lymphocytes[J]. Neuropsychiatr Dis Treat, 2015, 11: 1523-1540.
  30. [30].
    LI JY, ZHAO RZ, LI XL, et al. Shen-Qi-Jie-Yu-Fang exerts effects on a rat model of postpartum depression by regulating inflammatory cytokines and CD4＋CD25＋ regulatory T cells[J]. Neuropsychiatr Dis Treat, 2016, 12: 883-896.
  31. [31].
    刘颖楠，唐启盛，杨歆科，等. 基于网络药理学及实验验证探究参芪解郁方治疗产后抑郁症的作用机制[J]. 世界中医药，2023, 18(24): 3492-3499.

Lesen Sie die ganze Passage

The above content is generated by Large Model Translation. The translated content is for reference only. We do not assume any commercial or legal responsibilty for any consequences arising from the use of our website