Objective To establish a new gas chromatography（ GC） method coupled with ultrasoundassisted extraction（ UAE） and solid-phase microextraction（ SPME） for rapid determination of seven kinds of organophosphorus pesticide residues and three kinds of pyrethroid pesticide residues in 13 Miao ethnomedicines produced in Guizhou province. Methods Japanese Honeysuckle Flower（ Flos Lonicerae Japonicae

Jinyinhua） was the optimization object. SPME was used directly for enrichment of extracts obtained by ultrasonic-assisted solvent. Then gas chromatography-electron capture detector was used for the determination. Results Under optimum conditions

seven kinds of organophosphorus pesticides showed a good linear relationship at the range from 5 to 500 μg / L

and three kinds of pyrethroid pesticides did at the range from 2. 5 to 250 μg / L. The recovery was from 85. 6 % to 112. 7 % with the RSD3. 2%

9. 8%. The limit of detection was from 0. 02 to 0. 33 μg / L. Conclusion UAE-SPME-GC established is an easy

environmental-friendly and rapid method for simultaneous determination of residual amount of 10 organophosphorus and pyrethroid pesticides in Miao ethomedicines. This method with directly enriching and sampling

was a simplified sample pretreatment procedure without purification process.