To investigate the mechanism of Illicium henryi injection on rheumatoid arthritis based on JAK/STAT signaling pathway

Huijie ZHANG; Limin CHAI; Juan LIU; Pingxin ZHANG; Jinyu LI; Dongyang LI; Song SUN

doi:10.3969/j.issn.1006-2157.2022.03.010

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To investigate the mechanism of Illicium henryi injection on rheumatoid arthritis based on JAK/STAT signaling pathway

Chinese Medicinal Pharmacology | 更新时间：2022-04-01

- To investigate the mechanism of Illicium henryi injection on rheumatoid arthritis based on JAK/STAT signaling pathway
- Journal of Beijing University of Traditional Chinese Medicine Vol. 45, Issue 3, Pages: 267-274(2022)
- 作者机构：
  
  北京中医药大学东直门医院　北京　100700
- 作者简介：
  
  Prof. CHAI Limin, Ph.D., Doctoral supervisor. Dongzhimen Hospital, Beijing University of Chinese Medicine, No.5, Haiyuncang, Dongcheng District, Beijing 100700, China. E-mail: liminchai@hotmail.com
- 基金信息：
  
  Second Batch of Scientific Research Project of the National TCM Clinical Research Base of State Administration of Traditional Chinese Medicine(JDZX2015191)
- DOI：10.3969/j.issn.1006-2157.2022.03.010
  CLC： R285.5
- Received：06 August 2021，
  
  Published：30 March 2022
- 稿件说明：
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Huijie ZHANG, Limin CHAI, Juan LIU, et al. To investigate the mechanism of Illicium henryi injection on rheumatoid arthritis based on JAK/STAT signaling pathway[J]. Journal of Beijing University of Traditional Chinese Medicine, 2022, 45(3): 267-274.
DOI：

Huijie ZHANG, Limin CHAI, Juan LIU, et al. To investigate the mechanism of Illicium henryi injection on rheumatoid arthritis based on JAK/STAT signaling pathway[J]. Journal of Beijing University of Traditional Chinese Medicine, 2022, 45(3): 267-274. DOI： 10.3969/j.issn.1006-2157.2022.03.010.

摘要

目的

探讨红茴香注射液治疗类风湿关节炎的作用及机制。

方法

32只雄性DBA/1小鼠随机分为正常组、模型组、来氟米特组及红茴香注射液组，每组8只。采用尾根部及背部多点注射牛Ⅱ型胶原蛋白乳化剂建立胶原诱导性关节炎( CIA)小鼠模型。正常组和模型组以生理盐水、来氟米特组以来氟米特(3 mg/kg)灌胃，红茴香注射液组腹腔注射红茴香注射液(0.3 mL/kg)，每日1次，连续28 d。药物干预后每7天进行1次关节炎性评分，测量足跖关节肿胀厚度。28 d后取材，采用HE染色法观察踝关节组织病理学形态并进行组织病理学评分；采用Safranin O染色法观察关节软骨破坏程度并进行染色缺失评分；Western blot法测定滑膜组织中酪氨酸激酶2(JAK2)和信号转导及转录激活蛋白6(STAT6)、磷酸化酪氨酸激酶2(p-JAK2)、磷酸化信号转导及转录激活蛋白6(p-STAT6)的表达水平。

结果

与模型组比较，红茴香注射液组关节炎指数评分降低(

0. 05)，足跖关节厚度减小(

0. 05，

0. 01 )，关节病理改变评分及滑膜中JAK2、STAT6蛋白表达量及其磷酸化水平均降低(

0. 05，

0. 01)。

结论

红茴香注射液对CIA小鼠具有抗炎及免疫调节作用，其作用机制可能是通过抑制JAK2、STAT6相关蛋白磷酸化表达，调控JAK/STAT信号通路。

Abstract

Objective

We aimed to investigate the mechanisms underlying the effects of Illicium henryi injection on rheumatoid arthritis (RA).

Methods

Male DBA/1 mice were randomly divided into: (i) the normal group

(ii) the model group

(iii) the leflunomide group

and (iv) the Illicium henryi injection group (

= 8 mice per group). A collagen-induced arthritis (CIA) mouse model was established by multipoint injection of bovine type Ⅱ collagen emulsifier in the tail and back. Mice in the normal group and model group were given normal saline. Mice in the leflunomide group were given leflunomide (3 mg/kg) by gavage. Mice in the Illicium henryi injection group were intraperitoneally injected with Illicium henryi injection (0.3 mL/kg) once a day for 28 consecutive days. Joint inflammatory scores were recorded every 7 days after the intervention to measure the degree of joint swelling. After 28 days

HE staining was used to observe the histopathological morphology and record the histopathological score of the ankle joint. Safranin O staining was used to observe the degree of damage to articular cartilage and score the loss of staining. The protein expression levels of JAK2

p-JAK2

STAT6

and p-JAK6 in synovial tissues were determined by Western blot.

Results

Compared with the model group

the arthritis index score was lower in the Illicium henryi injection group (

0.05)

the foot joint was thinner (

0.05

0.01)

the pathological changes of the joint were less severe

and the expression and phosphorylation of JAK2 and STAT6 in synovium were lower (

0.05

0.01).

Conclusion

Illicium henryi injection has anti-inflammatory and immunomodulatory effects on CIA mice through inhibiting the phosphorylation and expression of JAK2 and STAT6 and regulating the JAK/STAT signaling pathway.

关键词

Keywords

references

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