Establishment of a mice model of the yin deficiency pattern of the liver and kidney and the effect of <italic style="font-style: italic">Gouqi Juhua Fang</italic> on asthenopia

Zhiqiang HUANG; Jianjun ZHANG; Danping ZHAO; Xue ZHOU; Chun WANG; Yan CHEN; Liang CHEN; Jun DU; Hongyue LIU; Guoyuan LI; Linyuan WANG

doi:10.3969/j.issn.1006-2157.2022.06.022

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Establishment of a mice model of the yin deficiency pattern of the liver and kidney and the effect of Gouqi Juhua Fang on asthenopia

Chinese Medicinal Pharmacology | 更新时间：2022-07-11

- Establishment of a mice model of the yin deficiency pattern of the liver and kidney and the effect of Gouqi Juhua Fang on asthenopia
- Journal of Beijing University of Traditional Chinese Medicine Vol. 45, Issue 6, Pages: 578-586(2022)
- 作者机构：
  
  1.北京中医药大学中药学院　北京　100029
  2.北京中医药大学中医学院
  3.安利(中国)研发中心有限公司
- 作者简介：
  
  Prof. WANG Linyuan, Doctoral Supervisor, Beijing University of Chinese Medicine. No.11 Beisanhuan Donglu Road, Chaoyang District, Beijing 100029. E-mail: wangly@bucm.edu.cn
- 基金信息：
  
  National Key R&D Program of China(2018YFC1706803)
- DOI：10.3969/j.issn.1006-2157.2022.06.022
  CLC： R285.5
- Received：19 November 2021，
  
  Online First：17 March 2022，
  
  Published：30 June 2022
- 稿件说明：
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Zhiqiang HUANG, Jianjun ZHANG, Danping ZHAO, et al. Establishment of a mice model of the yin deficiency pattern of the liver and kidney and the effect of Gouqi Juhua Fang on asthenopia[J]. Journal of Beijing University of Traditional Chinese Medicine, 2022, 45(6): 578-586. DOI: 10.3969/j.issn.1006-2157.2022.06.022.
DOI：

Zhiqiang HUANG, Jianjun ZHANG, Danping ZHAO, et al. Establishment of a mice model of the yin deficiency pattern of the liver and kidney and the effect of Gouqi Juhua Fang on asthenopia[J]. Journal of Beijing University of Traditional Chinese Medicine, 2022, 45(6): 578-586. DOI: 10.3969/j.issn.1006-2157.2022.06.022. DOI：

摘要

目的

通过建立肝肾阴虚证候小鼠模型研究具有缓解视疲劳作用的枸杞菊花方的滋补肝肾功效，以证测效，揭示枸杞菊花方缓解视疲劳的作用机制。

方法

50只雄性昆明小鼠按体质量随机分为正常组、模型组、杞菊地黄丸组(3.0 g/kg)、枸杞菊花方低剂量组(65.7 mg/kg)、枸杞菊花方高剂量组(131.4 mg/kg)，每组10只。每日上午，各干预组灌胃相应的药物，正常组和模型组灌胃等量蒸馏水，每日1次，共14 d。每日下午，模型组和各干预组灌胃左甲状腺素钠(150 μg/kg)，然后距尾尖约3 cm处夹尾5 min给予情志刺激，灌胃及夹尾均每日1次，共14 d。每日观察小鼠一般状态，第1、7、14天测量小鼠体质量。第14天检测温度趋向性。第15天取材，检测胸腺指数，血清环核苷酸、甲状腺激素、性激素、免疫因子水平，肝功能，肝能量代谢酶活力及氧化水平。

结果

与正常组相比，模型组小鼠出现体质量减轻、大便干硬、小便发黄、自主活动增加、易被激惹、反应活跃、喜冷怕热等虚热症状，杞菊地黄丸组和枸杞菊花方各组上述症状均有一定程度的改善。与模型组相比，枸杞菊花方低、高剂量组小鼠体质量增加(均

0.05)；血清环磷酸腺苷、环磷酸鸟苷、环磷酸腺苷与环磷酸鸟苷比值、雌二醇与睾酮比值、甲状腺素、谷丙转氨酶含量降低(均

0.05)，三碘甲腺原氨酸、白细胞介素－2升高(均

0.05)；胸腺指数升高(

0.05)；肝脏组织丙二醛含量、Na

＋

-K

＋

-ATP酶活力、Ca

2＋

-Mg

2＋

-ATP酶活力降低(均

0.05)。

结论

枸杞菊花方和杞菊地黄丸均可改善肝肾阴虚证候模型小鼠的阴虚内热和内分泌激素紊乱状态，降低能量代谢速率，增强机体免疫和抗氧化因子水平，综合缓解肝肾阴虚的状态，发挥滋养肝肾的中医功效，从而起到缓解视疲劳的功能。且由于枸杞菊花方用药安全，药少力专，有效成分明确，有开发成为安全、稳定、可控且具有中医辨证特色的缓解视疲劳产品的潜力。

Abstract

Objective

To verify and reveal the mechanism of traditional Chinese medicine (TCM) of

Gouqi Juhua Fang

(GJ

Fang

) on asthenopia

this experiment studied the nourishing effect of GJ

Fang

by establishing the yin deficiency of the liver and kidney pattern model.

Methods

According to body weight

50 male Kunming mice were randomly divided into five groups (each

=10): normal group

model group

Qiju Dihuang

Pill (QD Pill) group (3.0 g/kg)

low-dose GJ

Fang

group (65.7 mg/kg)

and high-dose GJ

Fang

group (131.4 mg/kg). Each morning

the intervention groups were administered corresponding medicine by gavage

and the normal group and model group were administered the same amount of distilled water by gavage

for 14 days. Each afternoon

the model group and each intervention group were given levothyroxine sodium solution (150 μg/kg) by gavage

and then the tail was clipped about 3 cm from the tip for 5 minutes

for 14 days. The general state of the mice was observed every day

and all mice were weighed on the 1st

7th

and 14th day. On the 14th day

the temperature tropism was detected. On the 15th day

all mice were sacrificed

and the samples were collected. The thymus index was calculated; the cyclic nucleotides

thyroid hormones

sex hormones

liver function

and immune factors in serum were detected; the energy metabolism enzyme activities and oxidation levels in the liver tissue were also determined.

Results

Compared with the normal group

the model group showed symptoms of deficiency and heat such as weight loss

dry and hard stools

yellow urine

increased voluntary activities

irritability

active reactions

and the desire to be close to the cold environment. The symptoms both improved to a certain extent in the QD Pill group and GJ

Fang

group. Compared with the model group

the low- and high-dose GJ

Fang

group showed the following: The bodyweight of the mice was increased (

0.05); the contents of cAMP

cGMP

cAMP/cGMP

and ALT were decreased (

0.05); the thymus index was increased (

0.05); and the levels of T3 and IL-2 increased (

0.05). In addition

the contents of MDA and the activities of Na

-K

-ATPase and Ca

-Mg

-ATPase decreased(

0.05) in the liver.

Conclusion

Fang

and QD Pill both can improve internal heat and endocrine hormone disorders in yin deficiency

reduce the rate of energy metabolism

enhance the immunity and antioxidant factors level

and comprehensively relieve the state of yin deficiency in the liver and kidney

thus relieving asthenopia. Because of the safe formula and clear active ingredients

Fang

has the potential to be developed into a safe

stable

controllable

and pattern-differentiated product for asthenopia.

关键词

Keywords

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Establishment of a mice model of the yin deficiency pattern of the liver and kidney and the effect of Gouqi Juhua Fang on asthenopia

DOI：10.3969/j.issn.1006-2157.2022.06.022

摘要

Abstract

关键词

Keywords

references