Effects of genistein on mitochondrial damage and G2/M phase arrest in breast cancer cells

CHAN Liujia; WANG Wenjing; PANG Yuheng; ZOU Xin; YI Bing; WANG Yuji; ZHAO Lichun

doi:10.3969/j.issn.1006-2157.2023.04.011

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Effects of genistein on mitochondrial damage and G2/M phase arrest in breast cancer cells

Experimental Studies | 更新时间：2023-05-09

- Effects of genistein on mitochondrial damage and G2/M phase arrest in breast cancer cells
- Journal of Beijing University of Traditional Chinese Medicine Vol. 46, Issue 4, Pages: 520-527(2023)
- 作者机构：
  
  1.广西中医药大学　南宁　530200
  2.北京市肝病研究所
  3.哈尔滨医科大学附属肿瘤医院
  4.首都医科大学
- 作者简介：
  
  Zhao Lichun, Researcher, Ph. D., Master’s Supervisor. Guangxi University of Traditional Chinese Medicine, No.13, Wuhe Dadao, Qingxiu District, Nanning 530200.E-mail: hyzlc@126.com
- 基金信息：
  
  National Natural Science Foundation of China(82072903);General Program of Guangxi Natural Science Foundation(2020GXNSFAA238021);Key projects of Guangxi Natural Science Foundation(2021GXNSFDA075008)
- DOI：10.3969/j.issn.1006-2157.2023.04.011
  CLC： R285.5
- Received：25 July 2022，
  
  Published：30 April 2023
- 稿件说明：
移动端阅览
CHAN Liujia, WANG Wenjing, PANG Yuheng, et al. Effects of genistein on mitochondrial damage and G2/M phase arrest in breast cancer cells[J]. Journal of beijing university of traditional chinese medicine, 2023, 46(4): 520-527.
DOI：

CHAN Liujia, WANG Wenjing, PANG Yuheng, et al. Effects of genistein on mitochondrial damage and G2/M phase arrest in breast cancer cells[J]. Journal of beijing university of traditional chinese medicine, 2023, 46(4): 520-527. DOI： 10.3969/j.issn.1006-2157.2023.04.011.

摘要

目的

研究金雀异黄素对乳腺癌细胞株线粒体损伤和G2/M细胞周期阻滞的作用。

方法

采用细胞增殖与活性检测－8(CCK-8)法检测金雀异黄素(12.5、25、50、100、150、200 μmol/L)作用48 h对MDA-MB－231和MCF－7细胞的增殖影响；碘化丙啶单染法检测金雀异黄素(50、100、150 μmol/L，48 h)对MDA-MB－231和MCF－7细胞周期的影响；JC－1、MitoTracker Orange CMTMRos和Hoechst 33342染色法检测金雀异黄素(50、100、150 μmol/L，48 h)对MDA-MB－231和MCF－7细胞线粒体功能及细胞凋亡的影响。

结果

与对照组相比，金雀异黄素给药组细胞增殖活力下降，不同浓度金雀异黄素对细胞增殖均有抑制作用(

0.05，

0.01)；金雀异黄素可诱导MDA-MB－231和MCF－7细胞发生凋亡，并将细胞周期阻滞在G2/M期(

0.05

0.01)；金雀异黄素可有效降低MDA-MB－231和MCF－7细胞线粒体膜电位，并降低线粒体活性(

0.05

0.01)。

结论

金雀异黄素可以抑制MDA-MB－231和MCF－7细胞的增殖，并使其周期阻滞在G2/M期，诱导细胞凋亡，其作用机制可能与诱导线粒体功能损伤有关。

Abstract

Objective

We aimed to study the effect of genistein on mitochondrial damage and G2/M cell cycle arrest in breast cancer cell strain.

Methods

The Cell Counting Kit-8 (CCK-8) assay was used to determine the proliferation of MDA-MB-231 and MCF-7 cells after administration of genistein(12.5

100

150

and 200 μmol/L) for 48 h. The effects of genistein (50

100

and 150 μmol/L for 48 h) on the cell cycle were detected by PI staining of MDA-MB-231 and MCF-7 cells. JC-1

MitoTracker Orange CMTMRos

and Hoechst 33342 staining assays were used to detect the effects of genistein (50

100

and 150 μmol/L for 48 h) on mitochondrial function and apoptosis in MDA-MB-231 and MCF-7 cells.

Results

Compared with the control group

the cell proliferation activity was decreased in genistein groups

genistein could inhibit the proliferation of cells in each concentration(

0.05

0.01). Genistein induced nuclear chromatin agglutination in MDA-MB-231 and MCF-7 cells

which indicated that apoptosis was promoted. Genistein arrested the cell cycle of MDA-MB-231 and MCF-7 cells in the G2/M phase (

0.05

0.01).Genistein decreased the mitochondrial membrane potential and mitochondrial activity in MDA-MB-231 and MCF-7 cells (

0.05

0.01).

Conclusion

Genistein could inhibit the proliferation of MDA-MB-231 and MCF-7 cells

induce cell cycle arrest in the G2/M phase

and induce apoptosis. The underlying mechanisms could be related with mitochondrial function modulation.

关键词

Keywords

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