Effects of <italic style="font-style: italic">Polygonatum cyrtonema</italic> Hua polysaccharide on C2C12 cells with cisplatin-induced myotube atrophy based on TMT proteomics

TANG Xueyang; ZHOU Rongrong; FANG Xiaoyang; HUANG Ao; ZENG Hongliang; XIE Jing; JIN Jian; ZHANG Shuihan

doi:10.3969/j.issn.1006-2157.2023.06.009

您当前的位置：

首页 >

文章列表页 >

Effects of Polygonatum cyrtonema Hua polysaccharide on C2C12 cells with cisplatin-induced myotube atrophy based on TMT proteomics

Experimental Studies | 更新时间：2023-07-05

- Effects of Polygonatum cyrtonema Hua polysaccharide on C2C12 cells with cisplatin-induced myotube atrophy based on TMT proteomics
- Journal of Beijing University of Traditional Chinese Medicine Vol. 46, Issue 6, Pages: 790-800(2023)
- 作者机构：
  
  1.湖南中医药大学科技创新中心　长沙　410208
  2.湖南省中医药研究院中药资源研究所
  3.湖南省中医药研究院附属医院
  4.湖南省中医药研究院医学实验动物中心
- 作者简介：
  
  ZHANG Shuihan, Ph. D., Researcher, Doctoral Supervisor. Institute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, No. 8, Yuehua Road, Yuelu District, Changsha 410013. E-mail: zhangshuihan0220@126.com
- 基金信息：
  
  National Natural Science Foundation of China(81673585);Hunan Innovative Province Construction Special Project(2021SK2012)
- DOI：10.3969/j.issn.1006-2157.2023.06.009
  CLC： R285.5
- Received：08 January 2023，
  
  Online First：18 May 2023，
  
  Published：30 June 2023
- 稿件说明：
移动端阅览
TANG Xueyang, ZHOU Rongrong, FANG Xiaoyang, et al. Effects of Polygonatum cyrtonema Hua polysaccharide on C2C12 cells with cisplatin-induced myotube atrophy based on TMT proteomics[J]. Journal of Beijing University of Traditional Chinese Medicine, 2023, 46(6): 790-800. DOI: 10.3969/j.issn.1006-2157.2023.06.009.
DOI：

TANG Xueyang, ZHOU Rongrong, FANG Xiaoyang, et al. Effects of Polygonatum cyrtonema Hua polysaccharide on C2C12 cells with cisplatin-induced myotube atrophy based on TMT proteomics[J]. Journal of Beijing University of Traditional Chinese Medicine, 2023, 46(6): 790-800. DOI: 10.3969/j.issn.1006-2157.2023.06.009. DOI：

摘要

目的

基于串联质量标签(TMT)标记的定量蛋白质组学研究黄精多糖改善顺铂诱导小鼠成肌细胞(C2C12细胞)肌管萎缩的蛋白质代谢轮廓，探讨黄精多糖的作用机制。

方法

将C2C12细胞培养至肌管，分为4组：正常组、顺铂组、黄精多糖组、顺铂＋黄精多糖组。按如下方法处理。正常组：不含血清的高糖DMEM培养基；顺铂组：不含血清的高糖DMEM培养基＋50 μmol/L顺铂；黄精多糖组：不含血清的高糖DMEM培养基＋0.200 mg/L黄精多糖；顺铂＋黄精多糖组：不含血清的高糖DMEM培养基＋50 μmol/L顺铂＋0.200 mg/L黄精多糖。各组均避光培养24 h。CCK8法检测C2C12细胞活力，免疫荧光法检测肌管直径和细胞融合指数，TMT蛋白质组学表征黄精多糖改善顺铂致C2C12细胞肌管萎缩的蛋白质代谢轮廓。

结果

CCK8法结果显示，黄精多糖可促进C2C12细胞活力，顺铂致C2C12肌管萎缩的造模浓度为50 μmol/L，黄精多糖的给药质量浓度为0.200 mg/L。免疫荧光法结果显示，与顺铂组比较，顺铂＋黄精多糖组萎缩肌管的直径和融合指数升高(

0.01)。蛋白质组学结果显示，以磷酸泛酰－半胱氨酸连接酶、双功能损伤控制性磷酸酶、2－磷酸－D－甘油酸水解酶、CCR4－NOT转录复合体亚基9为代表的43个蛋白在正常组、顺铂组和顺铂＋黄精多糖组3组间发生了连续性的调节变化，核糖体、鞘脂类代谢、鞘糖脂生物合成相关代谢通路调节在黄精多糖改善顺铂所致的C2C12肌管萎缩中作用明显。

结论

黄精多糖能改善顺铂所致C2C12肌管萎缩，其机制可能与下调鞘磷脂磷酸二酯酶2介导的神经酰胺积累有关。

Abstract

Objective

To investigate the protein metabolism profile of

Polygonatum cyrtonema

Hua polysaccharide (PCP) on cisplatin-induced myotube atrophy in mouse myoblasts (C2C12 cells) by tandem mass tag (TMT)-labeled quantitative proteomics

and to further explore the mechanism of PCP.

Methods

C2C12 cells were cultured into myotubes and divided into four groups: normal group

cisplatin group

PCP group

and cisplatin + PCP group. The cells in the normal group were administered serum-free high-glucose DMEM medium

the cells in the cisplatin group were administered serum-free high-glucose DMEM medium + 50 μmol/L cisplatin

the cells in the PCP group were administered serum-free high-glucose DMEM medium+ 0.200 mg/L PCP

the cells in the cisplatin + PCP group were administered serum-free high-glucose DMEM medium+ 50 μmol/L cisplatin + 0.200 mg/L PCP

and cells of all of the groups were cultured for 24 h in the dark. The viability of C2C12 cells was detected by the CCK8 method

the myotube diameter and cell fusion index were determined by an immunofluorescence method

and the protein metabolism profile of PCP that improved C2C12 myotube atrophy induced by cisplatin was characterized by TMT proteomics.

Results

CCK8 result showed that PCP promoted the viability of C2C12 cells

the modeling dose of cisplatin-induced C2C12 myotube atrophy was 50 μmol/L

and the mass concentration of PCP was 0.200 mg/L. The result of immunofluorescence showed that

compared with the cisplatin group

the diameter and fusion index of the myotubes were increased after PCP intervention (

0.01). Proteomic result showed that 43 proteins

including phosphopantothenate-cysteine ligase

pantothenate kinase 4

α-enolase and CCR4-NOT transcription complex subunit 9

underwent continuous regulatory changes among the normal group

cisplatin group

and cisplatin + PCP group. Regulation of metabolic pathways related to ribosomes

sphingolipid metabolism and sphingolipid biosynthesis contributed to the improvement of C2C12 myotube atrophy induced by cisplatin.

Conclusion

PCP improved C2C12 myotube atrophy induced by cisplatin

and its mechanism may be related to downregulating the accumulation of ceramide

which may be mediated by sphingomyelin phosphodiesterase 2.

关键词

Keywords

references

HIRSCH TZ , PILET J , MORCRETTE G , et al . Integrated genomic analysis identifies driver genes and cisplatin-resistant progenitor phenotype in pediatric liver cancer [J]. Cancer Discov , 2021 ， 11 ( 10 )： 2524 － 2543 .

VAN ALLEN EM , MOUW KW , KIM P , et al . Somatic ERCC2 mutations correlate with cisplatin sensitivity in muscle-invasive urothelial carcinoma [J]. Cancer Discov , 2014 ， 4 ( 10 )： 1140 － 1153 .

HAIN BA , XU H , WANING DL . Loss of REDD1 prevents chemotherapy-induced muscle atrophy and weakness in mice [J]. J Cachexia Sarcopenia Muscle , 2021 ， 12 ( 6 )： 1597 － 1612 .

MALLARD J , HUCTEAU E , BENDER L , et al . Development of skeletal muscle atrophy and intermuscular adipose tissue in patients with early breast cancer treated with chemotherapy [J]. Am J Physiol Cell Physiol , 2022 ， 323 ( 4 )： 1325 － 1332 .

MENG QY , TAN SJ , JIANG Y , et al . Post-discharge oral nutritional supplements with dietary advice in patients at nutritional risk after surgery for gastric cancer: A randomized clinical trial [J]. Clin Nutr , 2021 ， 40 ( 1 )： 40 － 46 .

黄帝内经·素问 [M]. 北京：人民卫生出版社， 2012 ： 167 － 169 .

李娟，杨柱，龙奉玺，等．国医大师刘尚义治疗癌性疲乏用药数据挖掘 [J]. 辽宁中医杂志， 2018 ， 45 ( 4 )： 703 － 706 .

丁大伟．益气养阴方联合甲羟孕酮治疗气阴两虚型肺癌恶病质的临床研究 [D]. 南京：南京中医药大学， 2012 .

周英雄．健脾除痰法在非小细胞肺癌(NSCLC)的疗效及与顺铂联合增效减毒的机理研究 [D]. 广州：广州中医药大学， 2011 .

SHEN WD , LI XY , DENG YY , et al . Polygonatum cyrtonema Hua polysaccharide exhibits anti-fatigue activity via regulating osteocalcin signaling [J]. Int J Biol Macromol , 2021 ， 175 ： 235 － 241 .

SAKAI H , ASAMI M , NAITO H , et al . Exogenous insulin-like growth factor 1 attenuates cisplatin-induced muscle atrophy in mice [J]. J Cachexia Sarcopenia Muscle , 2021 ， 12 ( 6 )： 1570 － 1581 .

LI JY , WANG XF , ZHOU RR , et al . Polygonatum cyrtonema Hua polysaccharides protect BV2 microglia relief oxidative stress and ferroptosis by regulating NRF2/HO－1 pathway [J/OL]. Molecules , 2022 ， 27 ( 20 )： 7088 [ 2023－01－04 ]. https：//www.mdpi.com/1420－3049/27/20/7088 https：//www.mdpi.com/1420－3049/27/20/7088 .

李亚，李潇，董青，等 . 新加良附方抑制胃癌AGS细胞增殖迁移及其初步生物信息学分析 [J]. 北京中医药大学学报， 2022 ， 45 ( 11 )： 1110 － 1118 .

胡子铃，王雨萱，郭昕琦，等 . 补阳还五汤干预脑缺血大鼠视束损伤的作用及关键药理途径的生物信息学分析 [J]. 北京中医药大学学报， 2022 ， 45 ( 7 )： 709 － 718 .

MA R , JIANG R , CHEN XN , et al . Proteomics analyses revealed the reduction of carbon- and nitrogen-metabolism and ginsenoside biosynthesis in the red-skin disorder of Panax ginseng [J]. Funct Plant Biol , 2019 ， 46 ( 12 )： 1123 － 1133 .

AGUILAR-GALVEZ A , PEDRESCHI R , CARPENTIER S , et al . Proteomic analysis of mashua ( Tropaeolum tuberosum ) tubers subjected to postharvest treatments [J/OL]. Food Chem , 2020 ， 305 ： 125485 [ 2023－01－04 ]. https：//linkinghub.elsevier.com/retrieve/pii/S0308814619316024 https：//linkinghub.elsevier.com/retrieve/pii/S0308814619316024 .

DING ZH , FU LL , TIE WW , et al . Highly dynamic, coordinated, and stage-specific profiles are revealed by a multi-omics integrative analysis during tuberous root development in cassava [J]. J Exp Bot , 2020 ， 71 ( 22 )： 7003 － 7017 .

杨欣．基于蛋白质组学中药寒热药性的研究 [D]. 哈尔滨：黑龙江中医药大学， 2016 .

王秋红，杨欣，李雪磊，等 . 基于蛋白质组学的附子、干姜和花椒的性味研究 [J]. 世界中医药， 2015 ， 10 ( 12 )： 1824 － 1836 .

CHEN CH , YAO XQ , XU YH , et al . Dahuang Zhechong Pill suppresses colorectal cancer liver metastasis via ameliorating exosomal CCL2 primed pre-metastatic niche [J/OL]. J Ethnopharmacol , 2019 ， 238 ： 111878 [ 2023－01－04 ]. https：//linkinghub.elsevier.com/retrieve/pii/S0378874118347202 https：//linkinghub.elsevier.com/retrieve/pii/S0378874118347202 .

LIN LF , LIU YL , FU S , et al . Inhibition of mitochondrial complex function-the hepatotoxicity mechanism of emodin based on quantitative proteomic analyses [J/OL]. Cells , 2019 ， 8 ( 3 )： 263 [ 2023－01－04 ]. https：//www.mdpi.com/2073－4409/8/3/263 https：//www.mdpi.com/2073－4409/8/3/263 .

王勇，啜文静，郭淑贞，等 . 基于小型猪冠心病心肌缺血模型的血瘀证蛋白组学研究 [J]. 北京中医药大学学报， 2011 ， 34 ( 7 )： 460 － 464 .

CHEN XZ , WU YP , YANG TH , et al . Salidroside alleviates cachexia symptoms in mouse models of cancer cachexia via activating mTOR signalling [J]. J Cachexia Sarcopenia Muscle , 2016 ， 7 ( 2 )： 225 － 232 .

KONDRASHOV N , PUSIC A , STUMPF CR , et al . Ribosome-mediated specificity in Hox mRNA translation and vertebrate tissue patterning [J]. Cell , 2011 ， 145 ( 3 )： 383 － 397 .

GOMEZ-ROMAN N , GRANDORI C , EISENMAN RN , et al . Direct activation of RNA polymerase Ⅲ transcription by c-Myc [J]. Nature , 2003 ， 421 ( 6920 )： 290 － 294 .

FERREIRA LF , MOYLAN JS , GILLIAM LA , et al . Sphingomyelinase stimulates oxidant signaling to weaken skeletal muscle and promote fatigue [J]. Am J Physiol Cell Physiol , 2010 ， 299 ( 3 ): 552 － 560 .

LUO S , PAN Z , LIU S , et al . Sphingomyelin synthase 2 overexpression promotes cisplatin-induced apoptosis of HepG2 cells [J]. Oncol Lett , 2018 ， 15 ( 1 )： 483 － 488 .

JAFFRÉZOU JP , LEVADE T , BETTAÏEB A , et al . Daunorubicin-induced apoptosis: triggering of ceramide generation through sphingomyelin hydrolysis [J]. EMBO J , 1996 ， 15 ( 10 )： 2417 － 2424 .

VERMA MK , YATEESH AN , NEELIMA K , et al . Inhibition of neutral sphingomyelinases in skeletal muscle attenuates fatty-acid induced defects in metabolism and stress [J/OL]. Springerplus , 2014 ， 3 ： 255 [ 2023－01－04 ]. https：//springerplus.springeropen.com/articles/10.1186/2193－1801－3－255 https：//springerplus.springeropen.com/articles/10.1186/2193－1801－3－255 .

Views

363

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Yiqi Yangyin Chujie Formula combined with cisplatin on the growth and apoptosis of human lung adenocarcinoma cell A549

Mechanism of treating osteoporosis with Zhuanggu Zhitong Fang based on proteomics

Proteomics of leukemia K562 cells treated by extracts of Yiyebaijiang（Patrinia heterophylla）

Influence of Siwu Mixture on metabolism in rats after chemotherapy by applying NMR metabonomics technique

Extraction,separation,composition and properties of polysaccharide from Humulus scandens

Related Author

QIAN Yanfang

HU Jinghong

ZHU Yuting

WEI Zhen

ZHANG Xiuxia

ZHAO Jiayi

SUN Hongmei

LIU Chang

Related Institution

Beijing University of Chinese Medicine

Navy General Hospital

Shaanxi University of Chinese Medicine

First People’s Hospital of Yuzhong County of Gansu Province

Hunan University of Chinese Medicine

AI问答

Postal code：100029
Tel：(010)53911857 Email：jbutcm@bucm.edu.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Effects of Polygonatum cyrtonema Hua polysaccharide on C2C12 cells with cisplatin-induced myotube atrophy based on TMT proteomics

DOI：10.3969/j.issn.1006-2157.2023.06.009

摘要

Abstract

关键词

Keywords

references