The effects of <italic style="font-style: italic">Epimedium koreanum</italic> Nakai on liver function in rats with kidney yang deficiency and kidney yin deficiency pattern models based on the " You Gu Wu Yun" theory

PENG Xiyi; ZHANG Lin; ZHAI Yuqi; YU Shuwei; TIAN Yuan; GUO Zhaojuan; YANG Song; WANG Ting

doi:10.3969/j.issn.1006-2157.2024.11.000

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The effects of Epimedium koreanum Nakai on liver function in rats with kidney yang deficiency and kidney yin deficiency pattern models based on the " You Gu Wu Yun" theory

Experimental Studies | 更新时间：2024-12-02

- The effects of Epimedium koreanum Nakai on liver function in rats with kidney yang deficiency and kidney yin deficiency pattern models based on the " You Gu Wu Yun" theory
- Journal of Beijing University of Traditional Chinese Medicine Vol. 47, Issue 11, Pages: 1562-1572(2024)
- 作者机构：
  
  1.北京中医药大学中医学院　北京　100029
  2.北京中医药大学中医药研究院
  3.北京中医药大学中药学院
- 作者简介：
  
  Prof. WANG Ting, Ph.D., Doctoral Supervisor. School of Chinese Materia Medica, Beijing University of Chinese Medicine, No.11, Beisanhuan Donglu Road, Chaoyang District, Beijing 100029. E-mail: wangting1973@sina.com.
- 基金信息：
  
  National Natural Science Foundation of China(82004035)
- DOI：10.3969/j.issn.1006-2157.2024.11.000
  CLC： R285.5
- Received：20 May 2024，
  
  Published：30 November 2024
- 稿件说明：
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PENG Xiyi, ZHANG Lin, ZHAI Yuqi, et al. The effects of Epimedium koreanum Nakai on liver function in rats with kidney yang deficiency and kidney yin deficiency pattern models based on the " You Gu Wu Yun" theory[J]. Journal of beijing university of traditional chinese medicine, 2024, 47(11): 1562-1572.
DOI：

PENG Xiyi, ZHANG Lin, ZHAI Yuqi, et al. The effects of Epimedium koreanum Nakai on liver function in rats with kidney yang deficiency and kidney yin deficiency pattern models based on the " You Gu Wu Yun" theory[J]. Journal of beijing university of traditional chinese medicine, 2024, 47(11): 1562-1572. DOI： 10.3969/j.issn.1006-2157.2024.11.000.

摘要

目的

基于“有故无殒”理论观察朝鲜淫羊藿对肾阳虚、肾阴虚及正常大鼠肝脏功能的影响，探究朝鲜淫羊藿致肝损伤与机体证候间的联系。

方法

采用随机数字表法将72只雄性SD大鼠分为正常组、肾阳虚组、肾阴虚组。肾阳虚组大鼠每日肌内注射氢化可的松注射液(20 mg/kg)建立肾阳虚证候模型，肾阴虚组大鼠每日灌胃甲状腺片混悬液(160 mg/kg)建立肾阴虚证候模型，连续14 d。造模成功后将大鼠按随机数字表法分为正常组，朝鲜淫羊藿低、高剂量组，肾阳虚组，肾阳虚＋朝鲜淫羊藿低、高剂量组，肾阴虚组，肾阴虚＋朝鲜淫羊藿低、高剂量组。药物组动物连续灌胃相应药物，朝鲜淫羊藿低剂量各组剂量为0.26 g/kg、朝鲜淫羊藿高剂量各组剂量为0.77 g/kg，其余组予饮用水，每日1次，连续28 d。给药期间监测大鼠体质量变化；给药结束后，采用生化分析仪检测各组大鼠血清碱性磷酸酶(ALP)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、直接胆红素(DBil)、间接胆红素(IBil)、总胆红素(TBil)含量；测量各组大鼠肝脏质量、计算脏体比及脏脑比；HE染色观察各组大鼠肝脏病理变化。

结果

与正常组比较，朝鲜淫羊藿高剂量组大鼠在给药第21天体质量降低，IBil升高(均

0.05)；肾阳虚组大鼠第3～28天每个测量时间节点体质量均降低，ALP升高，肝脏质量降低(均

0.05)。与肾阳虚组比较，肾阳虚＋朝鲜淫羊藿高剂量组大鼠ALP降低(均

0.05)，肾阳虚各给药组大鼠肝组织病理损伤情况有所改善，脂肪空泡情况减少，病理评分有降低趋势。与肾阴虚组比较，肾阴虚＋朝鲜淫羊藿高剂量组大鼠IBil升高，脏脑比降低(均

0.05)。

结论

在研究剂量范围内，朝鲜淫羊藿对肾阳虚证大鼠肝功能影响较小，对正常大鼠及肾阴虚证大鼠有一定致肝损伤作用，朝鲜淫羊藿致肝损伤与证候间可能存在相关性。

Abstract

Objective

This study explored the effects of

Epimedium koreanum

Nakai (EK) on liver function in normal

kidney yang deficiency (KYANGDS)

and

kidney yin deficiency (KYINDS) rats based on the theory of " You Gu Wu Yun." The study also investigated the connection between EK-induced liver injury and symptoms.

Methods

Seventy-two male SD rats were divided into normal

KYANGDS

and KYINDS groups using the random number table method. The KYANGDS model was established through intramuscular injection of 20 mg/kg of hydrocortisone

whereas the KYINDS model was established through daily gavage of 160 mg/kg of thyroid tablet suspension for 14 consecutive days. After successful modeling

the rats were divided into the normal

EK low-dose

EK high-dose

KYANGDS

KYANGDS + EK low-dose

KYANGDS + EK high-dose

KYINDS

KYINDS + EK low-dose

and KYINDS + EK high-dose groups using the random number table method. Animals in the drug groups were administered low (0.26 g/kg) and high (0.77 g/kg) EK extract doses through continuous gavage. The other groups received drinking water once a day for 28 consecutive days. Changes in body mass were monitored during the administration period

and serum alkaline phosphatase (ALP)

alanine transaminase(ALT)

aspartate transaminase(AST)

direct bilirubin

indirect bilirubin (IBil)

and total bilirubin (TBil) were measured at the end of administration using biochemical analyzers. The liver weight

visceral body ratio

and visceral brain ratio were measured. Hematoxylin and eosin staining were performed to observe the pathological changes in the liver.

Results

Compared with the normal group

the EK high-dose group showed a decrease in body weight on the 21st day during the drug administration period and an increase in IBil (

0.05); the KYANGDS group had lower body weight at each measurement time point from the third to the 28th day

higher ALP

and lower liver weight (

0.05). Compared with the KYANGDS group

the KYANGDS + EK high-dose group had decreased ALP levels (

0.05). The pathological damage of liver tissue

in each KYANGDS administration group improved

the presence of fat vacuoles were reduced

and pathological scores show a decreasing trend. Compared with the KYINDS group

KYINDS+ EK high-dose group exhibited a higher IBil level and a lower visceral brain ratio(

0.05).

Conclusion

EK has a small effect on the liver function of rats with KYANGDS within the dose range; however

it has a specific hepatogenic impact on normal and KYINDS rats

and EK-induced liver injury and the symptoms may be associated with each other.

关键词

Keywords

references

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The effects of Epimedium koreanum Nakai on liver function in rats with kidney yang deficiency and kidney yin deficiency pattern models based on the " You Gu Wu Yun" theory

DOI：10.3969/j.issn.1006-2157.2024.11.000

摘要

Abstract

关键词

Keywords

references