最新刊期
卷 0 ， 期 5 ， 2004
- 摘要：Apoplexy is a commonly-encountered disease and sometimes, its zang-fu diagnosis and treatment can not fully achieve desired clinical efficacy. It seems that the approach of diagnosing and treating collateral diseases can be adopted as a beneficial supplement for dealing with apoplexy. It is found that there exists the pathogenesis of collateral disease in the development of apoplexy, so that using the approach of diagnosing and treating collateral diseases to deal with apoplexy can help to increase the clinical efficacy. The commonly-seen TCM apoplectic syndromes differentiated by this approach are mainly attack of wind to collaterals, obstruction of collaterals by phlegm, obstruction of collaterals by stasis, harassment of cerebral collaterals by fire, detention of toxin in collaterals, attack of water to collaterals, and deficiency of both qi and blood of collaterals.关键词：Apoplexy;Collateral Disease;TCM Syndrome Differentiation;Differentiation and Treatment of TCM Syndromes
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发布时间：2021-09-15 -
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- 摘要：Objective To investigate the mechanism of Zhongyan No.Ⅱ for the treatment of AIDS in the monkeys. Methods Flow cytometry was used to detect and analyze the cell cycle, Western Blot analysis was used to detect the activity of mitogen activated protein kinase （ERK2）, and electrophoresis mobility shift assay was used to detect the activities of nuclear transcription factor-kB （NF-kB） and activator protien-1 （AP-1）. Results Zhongyan No.Ⅱ could block the lymphocytic function mediated by HI-SIV and HIVgp120, inhibit the activity of ERK2, decrease the activities of NF-kB and AP-1, and regulate and control the CD 3/CD 4 stimulation signal transduction pathway induced by HI-SIV. Conclusion The exertion of the effect of Zhongyan No.Ⅱ for regulating the function of T lymphocytes in the monkeys with AIDS may be through a signal cascade involving CD 4 co-stimulated T cell receptor signal pathway.关键词：Zhongyan No.Ⅱ;HIV-SIV;HIVgp120;CD 3/CD 4
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发布时间：2021-09-15 - 摘要：Objective To study the influence of Ganshenbo on the components of extracellular matrix of renal tissue, such as fibronetin （FN）, laminin （LN）, and collangen Ⅳ （Col Ⅳ）, and on the gene expressions of the extracellular matrix, in the rat with 5/6 nephrectomy （5/6 NT）. Methods Immunohistochemical techniques and hybridization in situ were used to detect FN, LN and Col Ⅳ and their mRNA expressions, TN-8502 multi-functional graph analysis system was used to process the experimental data, and SPSS statistical software was used to conduct the statistical processing. Results It was showed that there were no FN, LN and Col Ⅳ and their positive reactants of mRNA found in the rats in both the normal control group and sham-operation group; FN, LN and Col Ⅳ and their intensely-positive yellow reactants of mRNA were found in glomerular cytoplasm and tubular cytoplasm in the rats in the 5/6 NT group; FN, LN and Col Ⅳ and their positive reactants of mRNA were found decreased in the rats in the valsartan group, as compared with those in the 5/6 NT group （P<0.05）; and FN, LN and Col Ⅳ and their positive reactants of mRNA were found markedly decreased in the Ganshenbao group, as compared with those in the 5/6 NT group （P<0.01）.Conclusion One of the mechanisms of preventing and treating renal fibrosis of Ganshenbao is down-regulating FN, LN and Col Ⅳ and their gene expressions in the renal tissue.
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发布时间：2021-09-15 - 摘要：Objective To observe the effects of the extract of chicory on the levels of serum uric acid and lipid in the quail with hyperuricemia/hypertriglyceridemia. Method The quail model of hyperuricemia/hypertriglyceridemia was induced by feeding a kind of feedstuff prepared with yeast, and then the model quails were treated with the extract of chicory at a large dose, medium dose and a small dose, respectively, for the purpose of pharmacodynamic observation. Results It was found that the levels of serum uric acid and triglyceride were markedly increased in the model group while the level of serum cholesterol was not markedly changed, as compared with those in the normal control group. All the three doses of the extract of chicory showed marked decreasing effects on the levels of the serum uric acid and triglyceride in the model group while they did not show significant effect on serum cholesterol. Conclusion The extract of chicory possesses the effects of lowering the levels of serum uric acid and triglyceride, the mechanisms of which are waiting for a further study.关键词：Hyperuricemia/Hypertriglyceridemia;Yeast;Animal Model of Disease;Extract of Chicory;Quail
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发布时间：2021-09-15 - 摘要：Objective To observe the inhibitory effects of Hydroxysafflor Yellow A （HSYA）, which is an effective component of the dried flowers of Carthamus tinctorius used for activating blood and resolving blood stasis in TCM, on platelet activating factor （PAF）-induced rabbit platelet adhesion, 5-hydroxy tryptamine （5-HT） release, and increase in the level of free intraplatelet Ca 2+ of rabbit platelets. Methods Fluorospectrophotometry was used to detect the 5-HT released by the rabbit platelets activated by PAF, Fura-2 fluorospectrophotometry was used to observe the PAF-induced increase in the level of free intraplatelet Ca 2+ of rabbit platelets, and spectrophotometry was used to detect the PAF-induced enhancement of adhesion of rabbit platelets. Results The inhibitory effects of HSYA showed a marked dose-effect relationship when HSYA was used to treat PAF-induced enhancement of adhesion of rabbit platelets at doses of 479.6-1918.3 μmol/L, PAF-induced 5-HT release of rabbit platelets at doses of 280-1320 μmol/L, and PAF-induced increase in the level of free intraplatelet Ca 2+ of rabbit platelets at doses of 6.29-201.2 μmol/L. Conclusion HSYA can markedly inhibit PAF-induced enhancement of adhesion of rabbit platelets, PAF-induced 5-HT release of rabbit platelets, and PAF-induced increase in the level of free intraplatelet Ca 2+ of rabbit platelets.关键词：hydroxysafflor yellow A;Platelet Activating Factor;5-Hydroxy Tryptamine;Platelet Adhesion;Free Intraplatelet Ca 2+
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发布时间：2021-09-15 - 摘要：Objective To investigate the mechanism of the effects of Zhuangganzhuyu Decoction （ZD） on the occurrence and development of hepatic fibrosis. Methods The immunohistochemical staining technique was used to observe the effects of ZD on the conformation and function of hepatic stellate cells （HSC） of the rats with hepatic fibrosis induced by carbon tetrachloride-associated factors, and the image analysis technique and statistical method were used to process the data from the observation. Results ZD was found to have inhibitory effects on the expressions of α-SMA and Des.Conclusion ZD can inhibit the activation and proliferation of HSC, which exerts an effect of inhibiting the synthesis of extracellular matrix.关键词：Zhuangganzhuyu Decoction;Hepatic Stellate Cell;Activation;Image Analysis
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发布时间：2021-09-15 - 摘要：Objective To observe the effects of triptolide on the expressions of TNF-α and IL-10, the two cytokines of γδT lymphocytes in the peripheral blood of the rat with collagen-induced arthritis （CIA） in order to investigate the behavior of the γδT lymphocytes in CIA and the immunoregulation of triptolide over the γδT lymphocytes.Methods Wistar rats were used to induce the animal model of CIA by intradermally injecting bovine type II collagen at the back and the root of the tail. After being treated with triptolide, the peripheral blood was taken from the model rat for separating γδT lymphocytes. The flow cytometer and the TNF-α and IL-10 antibodies directly labeled with fluorescence were used to detect the expression of the intracellular cytokines of the γδT lymphocytes at the single-cell level. Results The percentage of γδT lymphocytes, （6.96±0.74）%, in the total lymphocytes; the percentage of the expression of intracellular TNF-α of γδT lymphocytes; （2.22±0.36）%; and the percentage of the expression of intracellular of IL-10, （1.00±0.20）%; were markedly decreased in the peripheral blood of the model rats, as compared with those in the normal control rats （P<0.01）. After the treatment with triptolide, all the three above-mentioned criteria were restored to their normal level. Conclusion Triptolide can exert an effect for treating rheumatoid arthritis, of which the mechanism may be its regulating the amount of γδT lymphocytes and the expressions of TNF-α and IL-10.关键词：Triptolide;Flow Cytometry;Collagen-Induced Arthritis;γδT Lymphocyte;rheumatoid arthritis
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发布时间：2021-09-15 - 摘要：Objective To observe the effects of Qidan Decoction （QD） on the vascular cell adhesion molecule-1 （VCAM-1） of the abdominal aorta, serum high density lipoprotein （HDL） and serum low density lipoprotein （LDL） in the rabbit with experimental arteriosclerosis, and to investigate the mechanisms of the effects. Methods The rabbit model of arteriosclerosis was induced by feeding a kind of high-lipid feedstuff. Different intervening factors were used to each experimental group. Immunohistochemical techniques were used to detect the expression of VCAM-1, and enzymological techniques were used to detect the levels of HDL and LDL.Results As compared with those in the model group, the expression of VCAM-1 was markedly decreased （P<0.01）, the level of serum LDL was significantly lowered （P<0.01）, and the level of serum HDL was markedly increased （P<0.01）. Conclusion QD can inhibit the development of arteriosclerosis by decreasing the level of LDL, increasing the level of HDL, and inhibiting the expression of VCAM-1 of abdominal aorta.关键词：Qidan Decoction;Arteriosclerosis;Vascular Cell Adhesion Molecule-1
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发布时间：2021-09-15 - 摘要：Objective To find out a method for establishing the animal model of experimental autoimmune myositis. Methods The animal model of experimental autoimmune myositis was induced by repeatedly immunizing the rats with guinea-pig skeletal muscle myosin （the concentration was 6 g/L and 3 g/ L, respectively） plus Freund’s complete adjuvant （containing BCG vaccine）, or giving a celiac injection of the stock solution of Bordetella pertussis （at a dose of 4.0×1010 bacteria of Bordetella pertussis each rat） in addition. The changes in serum muscle enzymes, serum myosin antibodies, electromyography parameters and other pathological respects were observed. Results It was found that the animal model of experimental autoimmune myositis induced by the above-mentioned procedure was similar to human polymyositis concerning the clinical symptoms, serum muscle enzymes, electromyography parameters and other pathological respects. Conclusion The animal model of experimental autoimmune myositis established in this experiment offers an important approach to the study of human polymyositis and is also a good animal model, which can be used to investigate the pathogenesis and treatment of human myositis.关键词：Polymyositis;animal model;immunity;Myosin
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发布时间：2021-09-15 - 摘要：Objective To observe the effects of Zibaishizhen Granules （ZG） against allergic reactions. Methods The 48-hour rat-homologous passive cutaneous anaphylaxis （PCA） was used to observe the effects of ZG on type Ⅰ allergic reaction; the reversed cutaneous anaphylaxis （RCA） was used to observe the effects of ZG on type Ⅱ allergic reaction; Arthus reaction was used to observe the effects of ZG on type Ⅲ allergic reaction; 2,4-Dinitrochlorobenzene （DNCB） method was used to observe the effects of ZG on type Ⅳ allergic reaction; and the effects of ZG on degranulation of mastocytes mediated by rat homocytotropic antibodies, and on the production of hemolysin were also observed in the experiment. Results Intragastric administration of ZG at a dose of 2.5 g/kg could markedly inhibit rat PCA （P<0 5）; the same administration of ZG at the doses of 1.5 g/kg and 2.5 g/kg could inhibit degranulation of mastocytes mediated by rat homocytotropic antibodies, wit the inhibitory rates as 37.8% and 41.5%, respectively. When being administered at the doses of 0 5 g/kg, 1.5 g/kg and 2.5 g/kg, ZG could inhibit rat RCA, with the inhibitory rates as 65 0%, 68 15 and 70.2%, respectively. ZG could also markedly inhibit rat Arthus reaction and mouse delayed type hypersensitivity induced by DNCB, and decrease mouse thymus index and spleen index. Conclusion ZG can exert significant inhibitory effects on the allergic reactions of type Ⅰ to type Ⅳ, and on degranulation of mastocytes mediated by homocytotropic antibodies.
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发布时间：2021-09-15 - 摘要：Objective To investigate the protective effects of Jiedutongluobaoshen Capsules （JC） against renal lesion in diabetic rats.Method The experimental animals were divided into the normal control group, diabetic control group, positive control group and experimental group. The levels of blood sugar, urea nitrogen, blood creatinine, and angiotensin Ⅱ （Ang Ⅱ） were measured, and the excretion rate （UAER） of urinary albumin and the index of renal hypertrophy were detected in each group at the 12 th week after the experiment. At the same time, RT-PCR was used to detect the mRNA expression of renal cortex connective tissue growth factor （CTGF）, and immunohistochemical techniques were used to detect the expressions of renal fibronectin （FN） and type IV collagen （COL Ⅳ）.Results In the JC-treated group, the blood sugar level was markedly lowered（P<0.01）, UAER was markedly reduced （P<0.05）, the levels of urea nitrogen and blood creatinine were decreased, and the renal hypertrophy index was improved（P<0.01）, as compared with those in the diabetic control group. The RT-PCR detection showed that the mRNA expression of renal cortex CTGF was increased and the sediment of glomerular FN and Col Ⅳ was raised in the diabetic control group, while in the JC-treated group, the expressions of CTGF mRNA, FN and Col IV were lower than those in the diabetic control group （P<0.05）. Conclusion The mechanism of the protective effects of JC against renal lesion may be decreasing the level of Ang Ⅱ in the renal tissue, downregulating the expression of CTCF, and reducing the sediment of extracelluar matrix.关键词：diabetes;connective tissue growth factor;Jiedutongluobaoshen Capsules;extracellular matrix;Angiotensin Ⅱ
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发布时间：2021-09-15 - 摘要：Objective To investigate the effects of Biejiaxiaozheng Pills （BP） for preventing and treating hepatic fibrosis. Method The rat model of chronic lesion-related hepatic fibrosis was induced by repeated subcutaneous injection of tetrachloromethane. The effects of different doses of BP were observed in the model rats concerning the histopathological changes in the hepatic tissue; the levels of serum hyaluronic acid, total protein, and albumin; the activities of serum alanine aminotransferase and serum aspartic acid aminotransferase; the weight of viscera; and the portal vein pressure. Colchicine was used as a control. Results It was found that all the doses of BP administered exerted certain protective effects on the rat liver, and their preventive effects showed in the experiment were better than those showed by colchicines. However, there was no significant difference found among the effects of different doses of BP. Conclusion BP can effectively prevent the tetrachloromethane-induced hepatic fibrosis in the rat.
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发布时间：2021-09-15 -
GC-MS Analysis of Chemical Components of the Volatile Oil from the Aerial Part of Bupleurum chinense
摘要：Objective To study the chemical components of the volatile oil from the aerial part of Bupleurum chinense.Method Water vapor distillation was used to extract the volatile oil from the aerial part of Bupleurum chinense, GC-MS was used to analyze and identify the chemical components of the volatile oil. Results The volatile oil was extracted from the aerial part of Bupleurum chinense for the first time, and 39 compounds were identified based on the 51 peaks appeared in the analysis. Conclusion The volatile oil accounts for 0.08% in the aerial part of Bupleurum chinense, and the main chemical component of the volatile oil is trans-caryophyllene-oxide, which is found existing in this Bupleurum plant for the first time.关键词：Aerial Part of Bupleurum chinense;volatile oil;GC-MS;chemical component- 12
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发布时间：2021-09-15 - 摘要：Objective To establish a method for quantitatively determining cinnamaldehyde in Aweihuapi Plaster. Method The HPLC procedure was used in the determination: the chromatographic column was Carbosorb ODS C18 （5 μm, 4.6 mm×150 mm）, the mobile phase was acetonitrile : water （30:70） with a flow velocity of 1.0 mL/min, the detection wavelength was 288 nm, and the room temperature was 25 ℃.Results It was showed that cinnamaldehyde had a good linear relationship in concentrations between 0.02 μg-0.60 μg, and the average recovery of the procedure was 96.0% （RSD =1.11%）. Conclusion This procedure is highly sensitive, and easy to be performed, which can be used in the quantitative determination of cinnamaldehyde in Aweihuapi Plaster.关键词：Aweihuapi Plaster;Cinnamaldehyde;HPLC;quantitative determination
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发布时间：2021-09-15 - 摘要：Objective To establish a HPLC method for quantitatively determining. danshensu in Rukuaixiao Tablets.Method The HPLC procedure was used in the determination: the mobile phase was methanol∶water∶acetonitrile∶glacial acetic acid （8∶92∶0.5∶1） and the detection wavelength was 280 nm. Results It was showed that danshensu had a good linear relationship in concentrations between 0.13 μg-1.96 μg （r=0.9998）, and the average recovery of the procedure was 101.19% （RSD=2.92%）. Conclusion This procedure is accurate and easy to be performed.关键词：Rukuaixiao Tablets;Danshensu;HPLC;quantitative determination
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发布时间：2021-09-15 - 摘要：Objective To optimize the alcohol extraction procedure of evodia fruits by the uniform design and orthogonal design, respectively, and to compare the two experiment design methods in terms of their feasibility. Method With the extraction rates of evodiamine and rutecarpine as criteria, the uniform design and orthogonal design were used, respectively, to examine the influence of such factors, as alcohol concentration, solvent volume and marinating time, on the extraction procedure. Result It was found that the optimized extraction procedure was to extract evodia fruits, the first time, with 8-fold of 70% alcohol for 2 hours, and the second time, with 7-fold of 70% alcohol for 1.5 hours. Conclusion The optimized extraction procedure is rationally designed with high extraction rates of evodiamine and rutecarpine, and it is suitable to be used in industrial production. The uniform design shows a higher feasibility in the present screening test on the extraction procedure of evodia fruits.关键词：Evodia Fruit;evodiamine;Rutecarpine;HLPC;Uniform Design;orthogonal design;Extraction Procedure
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发布时间：2021-09-15 - 摘要：Objective To optimize the base and antioxidant of Huaren Ointment. Method First, different bases and antioxidants were used in the preparation of Huaren Ointment. Then, the samples of Huaren Ointment were put in places with different temperatures, such as -15 ℃, room temperature and a constant temperature, respectively, for examining their changes in stability, oil-water separation, and color. Results It was found that the types and amounts of the bases and antioxidants used had notable influences on the stability of the samples of Huaren Ointment. Conclusion Huaren Ointment shows a good stability when the base is composed of stearic acid, triethanolamine, Tween-80, white oil, glycerin monostearate, and glycerol, and when NaHSO3 is used as the antioxidant.关键词：Base;Antioxidant;stability;Oil-Water Separation;Color Change
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发布时间：2021-09-15 - 摘要：Objective To evaluate the effects of the integrated therapy of TCM and western-style medicine （ITTCMWM） on pulmonary inflammation in SARS patients. Method To observe the lasting time of pulmonary inflammation by a same-period nonrandom control method in two groups of SARS inpatients （376 cases in all）, who received western-style therapy （WST） alone and the integrated therapy of TCM and western-style medicine, respectively. Results It was showed that the average absorption time of pulmonary inflammation in the ITTCMWM group was 2 days shorter than that in the WST group （Z=2.498, P=0.013）. The analysis of the absorption intensity of pulmonary inflammation （AIPI） showed that the AIPI in both general patients and severe patients in the ITTCMWM group was higher than that in both kinds of patients in the WST group （for the general patients, OR=0.763,P=0.01; for severe patients, OR=0.591, P=0.01）.Conclusion ITTCMWM helps to promote the absorption of pulmonary inflammation.关键词：SARS;Pulmonary Inflammation;Integration of TCM and Western-Style Medicine
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发布时间：2021-09-15 - 摘要：Objective To investigate the correlation between TCM syndromes and modern medical diagnostic criteria of apoplexy, in order to promote the TCM diagnostic level of apoplexy. Method 122 Inpatients of apoplexy at the acute stage were involved in this study. The patients were diagnosed by TCM diagnostic procedures within 72 hours of the onset according to the TCM Diagnostic Criteria for Apoplexy. A correlation study was conducted between TCM diagnoses and modern medical diagnostic criteria of apoplexy. The data were statistically processed by the method of multifactor cross analysis. Results TCM fire-heat syndrome, one of the 6 principal TCM syndromes of apoplexy, was significantly correlative with the risk factors of apoplexy in modern medicine, such as agedness, diabetes, and complications. It was also correlative with the state of rehabilitation, neurological state, and the ability in daily life activities of apoplectic patients （P<0.05）. Conclusion The patients of apoplexy are easy to develop complications and tend to be with poor ability in daily life activities and in an unsatisfied state of rehabilitation if they manifest the fire-heat syndrome within 72 hours of the onset of apoplexy.
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发布时间：2021-09-15 - 摘要：Objective To investigate the efficacy of the TCM therapy of enforcing spleen and nourishing kidney （TESNK） in the treatment of cognitive impairment in schizophrenia （CIS）. Method 60 Cases of CIS were randomly divided into the TCM group and the placebo group. 30 Normal subjects were included in the normal control group. The TCM group was given chloropromazine plus TCM drugs for enforcing spleen and nourishing kidney, the placebo group was given chloropromazine plus placebo, and the normal control group was not given any treatment. Patients in both the TCM group and placebo group were examined by the Digit Canceling Task scale, clinical Memory scales, and Wisconsin Card Sorting Test before and after they were treated, and the subjects in the normal control group were examined by the same methods. Results It was found that there was significant difference between the criteria of attention, memory and task performance of the patients in the TCM group and those of the patients in the placebo group （P<0.05）. Conclusion TESNK has an improving effect against cognitive impairment in schizophrenia.关键词：Schizophrenia;Cognitive Impairment;Enforcing Spleen and Nourishing Kidney
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发布时间：2021-09-15 - 摘要：Objective To investigate the clinical symptoms, signs, and the chest X-ray features of the patients of severe SARS, and the experience of the TCM treatment of severe SARS. Method The clinical symptoms, signs, chest X-rays and the restoration time of 26 patients of severe SARS at first visit were retrospected, and the results of the retrospective study were compared with the results of the retrospective study of the 30 patients of general SARS admitted in the same period concerning the above-mentioned criteria. Results In the patients with severe SARS, the commonly appeared clinical symptoms were found to be fever, dry cough, weakness, coughing with sputum, headache, short breath, and aching pain in muscle. Compared with those symptoms appeared in the patients of general SARS, the symptoms of weakness, short breath, and feeling disgusted with cold appeared at a higher frequency, and the symptoms of fever, weakness, headache, aching pain in muscle needed a longer time to be relieved. The results of the comparison were with statistical significance. Conclusion The pathogenesis of SARS is mainly of stagnation of heat toxin in lung, aggregation of stasis and heat, and impairment of qi and yin. Stickling to one single factor in its pathogenesis should be avoided in diagnosing SARS by TCM diagnostic methods, and the four natures, namely mildness, severity, meekness and acuteness, of the factors of its pathogenesis should be clearly understood in order to make a reasonable treating plan.关键词：SARS;Symptom and Sign;Integrated Therapy of TCM and Western-Style Medicine;Clinical Analysis
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发布时间：2021-09-15 -
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