最新刊期
卷 0 ， 期 1 ， 2005
- 摘要：Objective To carry out a study by using data-mining techniques in TCM quantitative diagnostics for promoting normalization and standardization of TCM diagnoses. Method Bayesian network method was used to make the quantitative diagnosis of blood stagnation syndrome based on TCM clinical diagnostic data of blood stagnation syndrome collected in 474 cases.Results 7 Key symptoms of blood stagnation syndrome were found out by using this method and their contribution degrees were calculated quantitatively. The rate of accurateness in diagnosing blood stagnation syndrome by a na?ve Bayesian classifier model based on these key symptoms was proved to be 96.6%.Conclusion The results suggest that the Bayesian network method has a promise of good future in making TCM quantitative diagnoses.关键词：TCM syndrome differentiation;Bayesian network;quantitative diagnostics
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发布时间：2021-09-15 -
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- 摘要：Objective To study the expressions of MARCKS and p-MARCKS in the hippocampal cortex in the rats with multiple acute cerebral infarct, observe the effects of 9601-Granules on MARCKS signal transduction pathway, and investigate the protective mechanism of TCM drugs against acute cerebral ischemia on the basis of proteins. Methods The rat model of multiple acute cerebral infarct was established by using modified Kaneko method; changes in the scoring of symptoms and signs of nervous system, and changes in the pathology and cell ultrastructure of hippocampal cortex, were observed in each experimental group; changes in the expressions of MARCKS and p-MARCKS in hippocampal cortex experienced acute ischemia and the regulating effects of TCM drugs on their expressions were detected by using immunohistochemical method and Western blot method. Results The levels of the expressions of MARCKS and p-MARCKS in hippocampal cortex were higher in the group experienced acute ischemia than those in the normal control group （P<0.05）. Although both 9601-Granules and Nimodipine could decrease the abnormal elevation in the expressions of MARCKS and p-MARCKS, there was no significant difference between the effects of the two drugs. Conclusion Under the condition of acute cerebral ischemia, the expressions of MARCKS and p-MARCKS are abnormally increased in hippocampal cortex in the rats, which is closely related with ischemic defect; and 9601-Granules possess marked downregulating effects on the abnormal increase.
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发布时间：2021-09-15 - 摘要：Objective To observe the inhibitory effect of two combinations of active fractions of Qixuebingzhi Formula, the active fractions D （mainly paeoniflorin） and E （mainly total flavones） blended according to a ratio based on the original formula and D and E blended according to an optimized ratio, on the smooth muscle cell （VSMC） proliferation caused by ox-LDL-induced vascular endothelial cell （VEC） injury-conditioned medium. Methods VSMC was cultured respectively in the normal VSMC culture fluid, ox-LDL-induced VEC injury-conditioned medium, simvastatin plus ox-LDL-induced VEC injury-conditioned medium, D and E blended according to the original formula plus ox-LDL-induced VEC injury-conditioned medium, and D and E blended according to an optimized ratio. MTT staining method was used to assess the growth state of VSMC and a flow cytometry method was used to detect the cell cycle of VSMC. Results After being cultured in VEC injury-conditioned medium, the activity of VSMC proliferation was markedly increased （P<0.01） and the number of S-phase cells was raised, as compared with the results from the normal control group; and the activity of VSMC proliferation was found to be markedly lower （P<0.01） and the number of S-phase cells was found to be reduced in the group of D and E blended according to the original formula （1 mg/L） and the group of D and E blended according to an optimized ratio （1 mg/L）, the effects of which were like the those of simvastatin. Conclusion Both combinations of the active fractions of Qixuebingzhi Formula can effectively inhibit VSMC proliferation induced by VEC injury-conditioned medium, to which the mechanism of Qixuebingzhi Formula for preventing and treating atherosclerosis is closely related.关键词：atherosclerosis;Endothelial Cell;Smooth Muscle Cell;oxidized low-density lipoprotein;Qixuebingzhi Formula
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发布时间：2021-09-15 - 摘要：Objective To investigate the effects of different ingredient-combinations of Xiaochaihu Decoction （XD） and the entire ingredients of XD on pleuritis induced by carrageenan. Methods Rat model of pleuritis was induced by intrathoracically injecting 0.2 mL of 1% carrageenan solution. Each XD group was intragastrically given a different XD ingredient- combination once a day: XD group A was given the root of Bupleurum chinense and the root of Scutellaria baicalensis （BCSB）, at 3.5 g/kg; XD group B was given the tuber of fresh ginger and Pinellia ternata （FGPT）, at 3 g/kg; XD group C was given ginseng, licorice and Chinese date （GLCD）, at 4.5 g/kg; XD group D was given BCSB plus FGPT, at 6.5 g/kg; XD group E was given BCSB plus GLCD, at 8 g/kg; XD group F was given FGPT plus GLCD, at 7.5 g/kg; and XD group G was given the entire ingredients of XD, at 11 g/kg. The normal group and the model group were intragastrically given the same dose of distilled water. The administration was lasted for 5 days, and the model of pleuritis was induced 2 hours after the last administration. The volume of pleurorrhea, the leukocyte count and the levels of LZM and MPO in the pleurorrhea, and the levels of NO and IL-8 in the serum, were examined 12 hours after the inducement of the model. Results Compared with those found in the model group, the volume of pleurorrhea, the leukocyte count and MPO level in the pleurorrhea, and the levels of IL-8 and NO were markedly decreased （P<0.01） in each XD group, and the level of LZM in the pleurorrhea was markedly increased （P<0.01）. Conclusion Both the entire ingredients of XD and different XD ingredient- combinations show a marked anti-inflammatory effect for treating and preventing pleuritis, with XD group A, XD group B, XD group C and XD group G manifesting a better effect. The mechanism for anti-inflammation of the entire ingredients of XD, BCSB, FGPT and GLCD may be checking the activation, infiltration and migration of leukocytes through inhibiting the increased levels of NO and IL-8.关键词：Xiaochaihu Decoction;formula designing;pleuritis;carrageenan
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发布时间：2021-09-15 - 摘要：Objective To investigate the influence of astrocyte-conditioned medium （ACM）, ACM treated with Kangdai-1, and ACM plus Kangdai-1 on the neurons damaged by simulated cerebral ischemia/reperfusion in vitro.Method First, the isolation, purification and cultivation of rat cortical astrocytes and neurons, and establishment of the model of stimulated cerebral damage by ischemia/reperfusion were carried out. Then, the ACM, ACM treated with Kangdai-1, and ACM plus Kangdai-1 collected 18 hours after ischemia/reperfusion, were used to culture the damaged neurons in a concentration of 1∶5. Last, the activity, survival rate and death rate of the cultured neurons, the culture fluid leakage rate of LDH, and the expressed amount of NOS-intense positive cells, were detected. Results All ACM, ACM treated with Kangdai-1, and ACM plus Kangdai-1 could markedly increase the activity and survival rate of the damaged neurons, and markedly decrease the death rate of the damaged neurons, the culture fluid leakage rate of LDH, and the expression of NOS-intense positive cells, with a sequence of potency as: ACM treated with Kangdai-1>ACM plus Kangdai-1>ACM. Conclusion （1） The neurons damaged by simulated cerebral ischemia/reperfusion in vitro showe a phasic change of damage-compensation-redamage-restoration; （2） ACM showes significant protective and repairing effects on damaged neurons; （3） Astrocytes play an important role in the process of brain ischemic preconditioning; （4） Kandai-1 may indirectly protect and repair damaged neurons through astrocytes.关键词：astrocyte;Neuron;cerebral ischemia/reperfusion;conditioned medium;Kangdai-1;brain ischemic preconditioning
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发布时间：2021-09-15 - 摘要：Objective To observe the protective effects of Naoxintong Capsules （NCs） against the cerebral damage in the rat experienced focal cerebral ischemia/reperfusion and on the cerebral infarct size and the cerebral levels of IL-1β,IL-6 and TNF-α. Methods The rat model of focal cerebral ischemia/reperfusion was used to observe the effects of NCs against the cerebral damage in the rat experienced focal cerebral ischemia/reperfusion and on the cerebral infarct size and the cerebral levels of IL-1β, IL-6 and TNF-α by using neuronal function behavior scoring, TTC staining, and ELISA. Results NCs doses of 0.24 g/kg and 0.48 g/kg could decrease the neuronal function behavior score （P<0.05）, reduce the cerebral infarct size （P<0.01）, and decrease the cerebral levels of IL-1β, IL-6 and TNF-α （P<0.05, P<0.01） in the rats in the model group. Conclusion The results suggest that NCs possess protective effects against the cerebral damage caused by focal cerebral ischemia/reperfusion, the mechanism of which is related to its decreasing the cerebral levels of IL-1β, IL-6 and TNF-α.关键词：Naoxintong Capsules;model of ischemia/reperfusion;IL-1β;IL-6;TNF-α
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发布时间：2021-09-15 - 摘要：Objective To study the mechanism of the therapy for nourishing qi, activating circulation and dispersing phlegm （NQACDP） for preventing and treating chronic obstructive pulmonary disease （COPD）. Method 72 Cases of COPD in a stable stage, manifesting a TCM syndrome of qi deficiency, blood stagnation and phlegm retention, were randomly divided into the treating group （35 cases） treated with oral NQACDP preparation, and the control group given nothing to intervene; changes in the expressions of serum TGF-β 1 and IL-8 in the patients in the two groups were observed; and a comparison was made between the changes in both criteria in the two groups. Results After treatment with NQACDP preparation, the levels of serum TGF-β 1 and IL-8 were markedly lower in the treating group, compared with those in the control group （P<0.01）; and the difference between the levels of serum TGF-β 1 and IL-8 in the two groups before and after the treatment was significant （P<0.05, P<0.01）. Conclusion NQACDP therapy may intervene airway structure remodeling in the process of progressive aggravation of COPD.关键词：therapy for nourishing qi, activating circulation and dispersing phlegm;chronic obstructive pulmonary disease;TGF-β 1;IL-8
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发布时间：2021-09-15 - 摘要：Objective To investigate the possibility of inducing cell proliferation in the penis tissue of Bushentongluo Recipe （BR） in emasculated rats. Methods 40 Adult male SD rats were randomly divided into the control group, emasculated group, BR group and testosterone propionate group. 2 Weeks after an operation for emasculation the BR group was given BR intragastrically and the testosterone propionate group was given testosterone propionate intramuscularly. The rats in all groups were executed 4 days and 1 week, respectively, after the treatment. The middle segment of the penis of the executed rats was taken for examining the cell proliferation by immunohistochemical techniques （SP method）. Results A cell proliferation to a medium degree was found in the penis tissue in the rats in the control group （35.07±2.94） but almost not any cell proliferation in the penis tissue was found 2 weeks after emasculation in the emasculated group （P<0.01）. However, DNA synthesis was induced 4 days and 1 week, respectively, after the treatment in the testosterone propionate group and BR group, with a marked increase in the cell proliferation in the penis tissue （20.60±2.66, 19.62±1.75, P<0.05）, including fibroblasts, smooth muscle cells and vascular endothelial cells, etc. Conclusion The results show that BR can induce proliferation of various types of cells in the penis tissue in the emasculated rats.关键词：Bushentongluo Recipe;androgen;cell proliferation;penis;rat
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发布时间：2021-09-15 - 摘要：Objective To study the protective effects of Naoxin Formula against brain function decline induced by D-galactose in mice. Methods The mouse model of brain function decline was induced by a subcutaneous injection of D-galactose （120 mg/kg for 46 days） at the mouse’s nape. A water maze detector was used to detect the mouse’s learning and memorizing abilities. At the same time, the activity of cerebral SOD and the levels of cerebral GSH and MDA were also examined. Results Intragastric administration of Naoxin Formula at doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg, respectively, for 46 days, could improve learning and memorizing abilities in the model mice to different degrees, markedly enhance the activity of cerebral SOD, increase the level of GSH, and decrease the level of MDA （P<0.05, P<0.01）. Conclusion One of the mechanisms of Naoxin Formula for adjusting abnormal behavior and treating senile dementia may be inhibiting cerebral lipid peroxidation and increasing the brain’s ability to resist lipid peroxidation.关键词：Naoxin Formula;D-galactose;lipid peroxidation;mouse
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发布时间：2021-09-15 - 摘要：Objective To establish a RP-HPLC method for quantitative determination of emodin in the vine of the plant of Sargentodoxa cuneata （Oliv.） Rehd. et Wils. Method DiamonsilTM C 18 column （200 mm×4.6 mm, 5 μm） was used as the chromatographic column, and methanol-0.1% aqueous solution of phosphoric acid was used as the mobile phase; the detection wavelength was 254 nm; the flow velocity was 1.00 mL/min; and the temperature of the column was 35℃. Result Emodin showed a good linear relationship when the amounts of the sample were between 0 0416～0 2080 μg （r=0.9997）, the average recovery was 101.4%, and the range of the content of emodin was found to be 6.72～13.71 μg/g.Conclusion This RP-HPLC method is simple, fast and accurate, with good repeatability.关键词：emodin;Sargentodoxa cuneata;RP-HPLC
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发布时间：2021-09-15 - 摘要：Objective To establish a method for quantitative determination of gensenoside Rg 1 and gensenoside Rb 1 in the effective fractions of Qingnaoxuanqiao Formula by RP-HPLC. Method The determination was carried out on a YWG-C 18 column （250 mm×4.6 mm, 10 μm）, and the detection wavelength was 203 nm. The mobile phase for gensenoside Rg 1 was CH 3CN-0.05%H 3PO 4 （21∶79） at a flow velocity of 1.2 mL/min, and the mobile phase for gensenoside Rb 1 was CH 3CN-0.05%H 3PO 4 （31∶69） at a flow velocity of 0.9 mL/min. Results The average recovery by this method for gensenoside Rg 1 was 100.71% （RSD = 1.16%, n=5）; the average recovery by this method for gensenoside Rb 1 was 99.32%, （RSD=2.52%, n=5）. Conclusion This method is simple and accurate, and can be used to control the quality of the effective fractions of Qingnaoxuanqiao Formula.关键词：Qingnaoxuanqiao Formula;gensenoside Rg 1;gensenoside Rb 1;RP-HPLC;quantitative determination
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发布时间：2021-09-15 - 摘要：Objective To investigate the dynamic change of chlorogenic acid, an effective component of Miyankang, in rabbits. Method The content of chlorogenic acid in the rabbit plasma was detected by a HPLC method: the mobile phase was methanol-water-glacial acetic acid-triethylamine （15∶85∶1∶0.3）, the stationary phase was YMC-C 18 chromatographic column, the detection wavelength was 324 nm, the flow velocity was 1.0 mL/min, and the temperature of the chromatographic column was 25℃. Result On the oral administration of Miyankang to the rabbit, the values of the pharmacokinetic parameters were found out to be: AUC=2.55 g/L·h, C max=0.88 g/L,t max=0.92 h, and MRT=1.90 h. Conclusion The model of chlorogenic acid absorption in the body of rabbits is of one-compartment with first-order absorption.关键词：Miyankang;effective component;chlorogenic acid;pharmacokinetics
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发布时间：2021-09-15 - 摘要：Objective To observe the effects of Tiaozhitongmai Granules （TGs） on serum lipids, lipoprotein metabolism, blood rheological criteria, and fibrinolytic system in senile patients of hyperlipoidemia. Method 96 Cases of senile hyperlipoidemia were randomly divided into two groups; the treating group （56 cases） was orally given TGs and the control group （40 cases） was given Xuezhikang Capsules. Result After treatment, the levels of serum total cholesterol （TC）, triglyceride, low density lipoprotein cholesterol （LDL-C）, apolipoprotein B, and lipoprotein （a） [LP（a）] were found decreased in both groups. The effects found in the treating group were better than those found in the control group concerning decreasing TC, LDL-C and LP（a）. The improving effects on atherogenic index and D-dimer found in the treating group were better than those found in the control group. Marked effects of improving blood rheological criteria were found in both groups. The improving effect on erythrocyte aggregation found in the treating group was better than that found in the control group. Conclusion TGs have been proved to possess the effects of regulating serum lipids and lipoprotein metabolism, and improving blood rheological criteria and fibrinolytic system in senile patients of hyperlipoidemia.关键词：Tiaozhitongmai Granules;hyperlipoidemia;aged people;nourishing qi, activating blood and resolving stagnation
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发布时间：2021-09-15 - 摘要：Objective To investigate the TCM symptoms and syndromes appeared in the patients with HIV/AIDS infection by blood selling. Method Epidemiological survey was adopted in the study, including enquiry to patients according to unified standards, filling-up of the questionnaire for symptoms and signs, finding-out of the frequencies of different symptoms, TCM syndrome classification by zang-fu syndrome differentiation, statistical data processing by using SPSS10.0 software, finding-out of occurrence rates of different TCM syndromes, and carrying-out of etiological analysis.Results Commonly seen TCM symptoms of the patients with HIV/AIDS infection mainly by blood selling were in such a sequence as weakness, impatience and easy to lose temper, dry mouth, low mood, cough, forgetfulness, sensitive to cold and cold limbs, anorexia, spontaneous perspiration, aching and weak waist and knees, insomnia, diarrhea, hair loss, and fever. The most commonly seen tongue patterns were dark red tongue proper, and curdy and greasy tongue fur. The frequently encountered pulse patterns were compound pulse patterns with a string-pulse feature, and the less frequently seen pulse patterns were thin pulse, deep pulse, fast pulse, slippery pulse, and weak pulse, respectively. Main TCM syndromes were found to be compound syndromes; spleen deficiency was the most commonly seen, and the less commonly seen were liver deficiency, qi deficiency, yang deficiency, internal damp-heat, heart deficiency, and liver depression and qi stagnation, respectively. Conclusion Clinically, the commonly seen TCM syndromes of the patients with HIV/AIDS infection by blood selling are syndromes of deficiency type, which mainly involve the liver, spleen and heart.关键词：HIV/AIDS;TCM syndrome;epidemiological survey;analysis
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发布时间：2021-09-15 - 摘要：Objective To investigate the significance of the Lab color model in TCM digital tongue diagnostics. Method The Lab color model was used to analyze the distribution of the digital tongue colors in 884 cases from the persons received a general physical examination, the results of which were compared with the tongue color information clinically collected by TCM physicians. Results The digital tongue color average values of L, a and b were 59.5, 27.9 and 15.5, respectively. Compared with the tongue color information clinically collected by TCM physicians, it was found that there was a negative correlation between L and b values and tongue color in the process of the change of the tongue colors from pale white to dark purple, whereas a positive correlation was found between the value of a and the tongue color, but without statistic significance. Conclusion The Lab color model still needs further improvement on the basis of clinical practice of the digital tongue diagnostics关键词：tongue diagnostics;tongue color;Image Analysis;Lab color model
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发布时间：2021-09-15 - 摘要：Objective To find out clinical characteristics of the symptoms and regularity of pathological development of SARS according to TCM syndrome differentiation, and to investigate the role of tongue patterns in preventing and treating SARS as well as the relationship between tongue patterns and changes in the blood pictures in the SARS patients. Method A prospective approach was adopted to generalize and statistically analyze the clinical manifestations, photos of tongue patterns, and results of the examination of the blood pictures in 214 SARS inpatients. Results It was found that there was a marked positive correlation between SARS patient white blood cell count and neutrophil percentage, and the condition of the tongue fur, such as curdiness and greasiness （P<0.01）; there was a marked negative correlation between lymphocyte percentage, eosinophil percentage and monocyte percentage, and the condition of the tongue fur, such as curdiness and greasiness （P<0.01）; there was a marked positive correlation between eosinophil percentage and the condition of the tongue proper, such as prickliness, flawing and red tip （P<0.01）; there was a marked positive correlation between monocyte percentage and the condition of the tongue proper, such as toughness, tenderness, redness, moisture, dryness and denudation （P<0.01）; there was a marked negative correlation between monocyte percentage and pale-redness of the tongue proper （P<0.01）; there was a marked positive correlation between blood platelet count and the condition of the tongue proper, such as shrinking, enlargement, and flawing （P<0.05）; and there was a marked negative correlation between blood platelet count, and redness of the tongue proper and thickness and thinness of the tongue fur （P<0.05）. It was also found that there was no obvious relationship between the patients’ tongue patterns, and ages and sexes. Conclusion There are specific changes in the tongue patterns and blood pictures in SARS patients, and among them, the tongue patterns have certain correlation with blood pictures. The dynamic observation can foretell changes in pathogenic development, which suggests that TCM tongue diagnostics can play an important role in the diagnosis and treatment of SARS.关键词：Severe Acute Respiratory Syndrome;TCM tongue pattern;peripheral blood picture
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发布时间：2021-09-15 -
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- 摘要：Objective To investigate the regulating effect of moxa-cone moxibustion on the phagocytic function of macrophages, in order to provide experimental data for the study of other moxibustion methods. Method 130 Mice were divided into the normal control group, moxibustion group, hypoimmune-model group, and hypoimmune-plus-moxibustion group. Flow cytometry and smear examination were adopted to observe phagocytosis of macrophages in these groups. Results Moxa-cone moxibustion of Dazhui acupoint had no big influence on phagocytosis of macrophages in the normal mice whereas it exerted marked enhancing effect on phagocytosis of macrophages in the hypoimmune mice. Conclusion Moxa-cone moxibustion possesses homeostatic effect.关键词：moxa-cone moxibustion;Dazhui acupoint;hypoimmunity;macrophage;phagocytic rate
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发布时间：2021-09-15 -
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