最新刊期
卷 0 ， 期 12 ， 2006
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- 摘要：Objective To explore the modern research methods for TCM symptomatology.Method The data mining techniques of TCM symptomatology were summarized from different TCM magazines in recent years,and their advantages and disadvantages were analyzed.Result The result showed that the（methods） for data mining in TCM symptomatology included association rules,set pair analysis,rough set theory,cluster analysis,artificial neural network,decision tree,support vector machine and Bayes（network）,etc.Conclusion The TCM data have the characteristics of nonlinearity,indistinction,（complicacy） and unquantification and so on.These methods should be applied integratedly in accordance with the specific TCM data and different mining aims,and their advantages will be given a full play to.关键词：TCM syndrome;data mining technique;methodology
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发布时间：2021-08-10 -
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- 摘要：Objective To study the change characteristics of gene expression of hippocampus in rats with congestive heart failure after heart infarction.Method The model of heart infarction was established by ligation of left front descending coronary artery and computer chip technique of gene spectrum was used to study the changes of gene expressions in the hippocampus and cortex in rats with congestive heart failure for 10 days and for 60 days respectively.Result The changes of gene expression in 10-day group was more active than those in 60-day group.There were 24 genes in hippocampus in 10day group and 14 genes in 60-day group.These genes were mainly related to genes of energy metabolism,neurotransmitters（metabolism）,sphingoin,signal transduction,stress and immune reaction.Among these genes the（expressions） of energy metabolism genes,such as pyruvate kinase 3（NM053297） and HMG-CoA lyase（NM024386） were decreased from 10th day of congestive heart failure,and the expressions of（neurotransmitters） metabolism genes,such as cholinergic receptor（nicotinic,epsilon polypeptide,（NM017194））,endosulfine alpha（NM021842） and glutamate oxaloacetate transaminase 2（（NM013177）） were decreased from 10th day of congestive heart failure.Conclusion The genes of（energy） metabolism and neurotransmitters metabolism were changed in brain hippocampus in primary（congestive） heart failure.关键词：congestive heart failure;gene expression;energy metabolism;neurotransmitters metabolism;rat
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发布时间：2021-08-10 -
- 摘要：Objective To study the mechanism of neuroimmunologic regulation of Danakang（EGb761） in rat model of Alzheimer’s disease（AD） induced by β-amyloid protein（Aβ）.Methods The AD rat model was established by injecting Aβ25～35 into rat bilateral amygdale.The spatial memory ability of AD model in Morris water maze（MWM） was observed,the expressions of glial fibrillary acidic protein（GFAP） and APPmRNA in AD rats were studied by using immunohistochemistry and RT-PCR,the（changes） of IL-1β and IL-6,and the influence of EGb761 were observed.Result The spatial memory（ability） of AD model in MWM was decreased.The expressions of APPmRNA,GFAP,IL-6 mRNA and（IL-1β） in the cortex and hippocampus of AD model were increased.The result showed that EGb761（improved） the oriental learning and memory ability, decreased the expressions of GFAP in hippocampus and cortex and APPmRNA expression of hippocampus in AD rats.Conclusion EGb761 can relieve（significantly） the disorder of learning and memory ability induced by Aβ in AD rat model,decrease APP mRNA（expression） of hippocampus,and alleviate the inflammation and immune reaction of neurons.One of the important mechanisms of EGb761 may be related to that it can control Aβ immune cascade reaction in the brain of AD patient.关键词：Danakang;b-amyloid protein;glial fibrillary acidic protein;IL-1;IL-6;β-amyloid（precursor） protein（β-APP）;rat
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发布时间：2021-08-10 - 摘要：Objective To observe the influence of salvianolic acid B（Sal B） on the energy metabolism of cerebral ischemia in mice.Methods The mice were randomly divided into the sham-operated group,cerebral ischemia group,Sal B group and Nimodipine（Nim） group.Brain tissue was collected to determine the mitochondria membrane potential（MMP）,energy charge（EC）,content of phosphocreatine（PCr）,activities of Na+-K+-ATPase and Ca2+-ATPase.Results In cerebral ischemia group MMP was significantly increased after cerebral（ischemia） for 20 and 30（minutes）（P<0.01）.The increase of MMP（after） cerebral ischemia was（abated） obviously in SalB group and Nim group（P<0.01）.In cerebral（ischemia） group the contents of EC and PCr of brain tissue were decreased remarkably 30 minutes after（cerebral） ischemia（P<0.05～0.01）,while those were（increased） in SalB group and Nim group（P<0.01）.The activities of Na+-K+-ATPase and Ca2+-ATPase in cerebral ischemia group were decreased（evidently） after cerebral ischemia for 20 and 30 minutes（P<0.01）,but the decrease was markedly（regulated） in SalB group and Nim group（P<0.01）.Conclusion The mechanism that SalB can improve the energy metabolism of cerebral ischemia might be related to its different regulative effects.关键词：salvianolic acid B;cerebral ischemia;energy metabolism;mice
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发布时间：2021-08-10 - 摘要：Objective To observe the effect of cinnamyl aldehyde（main principle of Cortex einnamomi） on the proliferation of NIH3T3 cells.Methods The effect of cinnamyl aldehyde in different concentrations on the proliferation of NIH3T3 cells was observed by using MTT assay,and on NIH3T3 cell cycle,by using flow cytometer.Result The cell proliferation rate（15%） increased obviously 48 hours after cinnamyl aldehyde acting on NIH3T3 cells（P<0.01）.The result of flow cytometer assay showed that the percentage of NIH3T3 cells in S phase was increased by 3%（P<0.05）,the percentage in G2/M phase was not increased and proliferate index（PrI） was increased by 3.5%（P<0.01） 24 hours after the action of cinnamyl aldehyde.Conclusion Cinnamyl aldehyde can improve the（proliferation） of NIH3T3 cells,which may be related to that cinnamyl aldehyde can promote more cells（into） S phase through regulating the cell cycle.关键词：fibroblast;cinnamyl aldehyde;cell cycle;proliferation
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发布时间：2021-08-10 - 摘要：Objective To observe the preventive effect of the total saponin of Radix Codonopsis on the（lesion） of cultured primary astrocytes（Ast） in rats induced by hypoxia and hypoglycemia reoxygenation.Methods The primary Ast of rat brain were cultured in Wistar rats（one day old） and then treated with Earle’s solution to establish the model of hypoxia and hypoglycemia.The cell survival rate was detected by MTT assay.The cell morphological changes,necrotic rate and apoptotic rate were observed by（orthotopic） double staining of hoe33342 and propidium iodide（PI） with the fluorescence microscope.The leakage rate of lactated dehydrogenase（LDH） was detected by chromatoptometry.Results The cell survival rate was significantly increased in the total saponin of Radix Codonopsis group I and III compared with that in the model group（P<0.01）.The necrotic rate was decreased significantly in the total（saponin） of Radix Codonopsis group I,II and III（P<0.01）. The apoptotic rate was not changed in these groups（P>0.05）,but the leakage rate of LDH was decreased significantly（P<0.01）.Conclusion The total saponin of Radix Codonopsis has a remarkable inhibitory effect on the necrosis of Ast after（hypoxia） and hypoglycemia reoxygenation lesion but no preventive effect on apoptosis,which indicates that the total saponin of Radix Codonopsis is the main active principle in Radix Codonopsis for treatment of（acute） stroke.关键词：Radix Codonopsis;the total saponin of Radix Codonopsis;astrocytes;hypoxia and hypoglycemia;rat
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发布时间：2021-08-10 - 摘要：Objective To study the regulation law of Chinese herbal drugs to catrtilage cell activity and metabolism,and discuss the machenism of Chinese herbal drugs for treating ostearthritis.Methods The metabolism changes of cartilage cells of rabbit knee joint were observed from the aspects of cell（proliferation）（MTT assay）,cell DNA synthesis（3H-TdR incorporation rate）,protein polysaccharides formation（toluidine blue（staining）） and collagen II synthesis（3H-proline incorporation）.Results The velvet antler polypeptides preparation and Viartrils Capsules had the improvement effect and Yiqitongluo Formula had the inhibitory effect on cell proliferation and polysaccharides formation.The improvement effect on cartilage cell（proliferation） in Yiqitongluo Formula group,velvet antler polypeptides group and Viartrils Capsules group had a significant difference compared with that in the control group（P<0.05）.The improvement effect on cartilage cell DNA and collagen II synthesis in velvet antler polypeptides group and Viartrils Capsules group had a significant difference compared with that in the control group（P<0.05）. Partilage cell（metabolism） was inhibited in Yiqitongluo Formula group（P<0.05）.Conclusion Yiqitongluo Formula blocks the catabiosis cytokines,prevents collagen fibers change and promotes cell proliferation.The（velvet） antler polypeptides preparation improves cartilage cell anabolism and promotes cartilage cell（proliferation）,and Viartrils Capsules protects cartilage cells and promotes cartilage cell（proliferation）.关键词：Yiqitongluo Formula;velvet antler polypeptides preparation;cartilage cells metabolism;rabbits
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发布时间：2021-08-10 - 摘要：Objective To observe the influence of Fufang Ruikangxin Granules on [Ca2+]i of morphine dependent SK-N-SH cell of human neuroblasoma and the mechanism.Method After the cell cycle（synchronized） SK-N-SH cells were treated with morphine of 10 μmol/L for 48 hours to establish the（morphine） dependent model.The model was intervened with Fufang Ruikangxin Granules serum for 12 hours,and then abstained by naloxone.The fluorescent intensity of free Ca2+ in SK-N-SH cells was（detected） by flow cytometer after the cell stained with Fluo-3-AM.Result The fluorescent intensity of cell free Ca2+ was decreased in high dose and medium dose groups of Fufang Ruikangxin Granules.The difference was significant compared with the blank group（P<0.05）.Conclusion The abstinence effect of Fufang Ruikangxin Granules may be related to it can decrease the level of free Ca2+ in cells.关键词：Fufang Riukangxin Granules;morphine dependent;pharmacology of serum with Chinese herbal drugs;cell Ca2+;SK-N-SH cells
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发布时间：2021-08-10 - 摘要：Objective To investigate the protective effect and the mechanism of Ginkgolide B Injection on rat model of brain local ischemia reperfusion lesion.Method The rat model of ischemia（3 hours） and reperfusion （21 hours） induced by thread approach were established for observing the effect of Ginkgolide B Injection on the neurological symptoms,infarction size,the activities of SOD,GSH-PX,Na+-K+-ATPase and Ca2+-My2+-ATPase, and the contents of MDA,CAT,GSH and NO.Result Ginkgolide B（Injection） in the doses of 8 mg/kg and 4 mg/kg relieved obviously the neurological symptoms and（infarction） in rats,the inhibitory rates were 29.6% and 23.4% respectively（P<0.01,P<0.05（compared） with those of the model group）,meanwhile improved the activities of SOD and GSH-PX,（increased） the contents of GSH and CAT,decreased MDA and NO contents,and promoted obviously the activities of Na+-K+-ATPase and Ca2+-My2+-ATPase（P<0.05,P<0.01） in the brain after ischemia and reperfusion lesion.Conclusion Ginkgolide B Injection has the protective effect on rats with cerebral local ischemia and reperfusion lesion,which may be related to that Ginkgolide B Injection can decrease NO content,antagonize lesion of free radicals and improve energy metabolism.关键词：Ginkgolide B Injection;cerebral ischemia;ischemia reperfusion;rat
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发布时间：2021-08-10 - 摘要：Objective To observe the wave characteristics and effective factors of portal pressure.（Methods） The body weight,hepatic coefficient,liver function,hepatic pathological changes,and portal pressure and wave were observed in model rats with hepatic fibrosis induced by CC14 and treated with（colchicines）.The sensitivity of portal pressure wave reflecting pathological indexes was analyzed.Results The indexes,such as body weight,hepatic coefficient,liver function and hepatic pathological changes（indicated） that the model of hepatic fibrosis induced by CC14 was established and the treatment with（colchicines） was effective.In the model group the maximum,mean and minimum of portal pressure（increased） respectively by 3.9,4.1 and 4.1 times compared with those in the control group,while in（colchicines） group those increased respectively by 4.8,5.3 and 5.1 times compared with those in the control group,but decreased by 19.2%,21.9% and 20.5% respectively than those in the model group.The area under each peak curve in portal pressure wave increased respectively by 5.1 and 4.3 times in the model group and colchicines group compared with that in the control group,while that decreased in colchicines group by 15.8% than that in the model group.The peak value in colchicines group was 2.3 times higher than that in the model group and control group.The maximal ascending velocity was equal to the maximal descending velocity.The maximal change velocity in the model group and colchicines group was obviously higher than that in the control group.Conclusion The portal pressure and wave,as an index of portal circulation,are sensitive and stable,and reflect the curative effect and regulation of（Chinese） herbal.关键词：portal hypertension;wave characteristics;hepatic fibrosis;rats
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发布时间：2021-08-10 - 摘要：Objective To study the operation rules of penetrating needling therapy in treatment of knee osteogonarthritis,and to analyze and evaluate comprehensively the clinical effect of this therapy.（Method）The penetrating needling therapy was used to puncture in the acupoints of Yanglingquan（GB 34） to（Yinlingquan）（SP 9）,Waixiyan（EX-LE 5） to Neixiyan（EX-LE 4）,Xiyangguan（GB 33） to Ququan（LR 8） and Liangqui（ST 34） to Xuehai（SP 10） to treat knee osteogonarthritis.The curative effect was observed by using the method of evidence-based medicine.Result There was a great significant （difference） between two groups after the evaluation and comparison of curative effect in two groups.There was a significant difference in the curative effect on pain between two groups.The group of penetrating needling therapy had better result than the group of acupuncture.Conclusion The penetrating needling therapy and filiform needling therapy all have effect in relieving pain and comprehensive curative effect on knee osteogonarthritis,while the penetrating needling therapy has a better effect than filiform needling therapy.关键词：penetrating needling therapy;filiform needling therapy;knee osteogonarthritis;curative effect evaluation
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发布时间：2021-08-10 - 摘要：Objective To study the relationship between the decocting quantity of liquiritigen or isoliquiritigenin and different formula combinations of Sini Powder.Method The reversed phased-high performance liquid chromatography（RP-HPLC） was used to determine the decocting quantity of（liquiritigenin） and isoliquiritigenin from the different combinations of Sini Powder,and then the result was analyzed statistically.Result The decocting quantity of liquiritigen and isoliquiritigenin were higher from the whole formula of Sini Powder than from other formula combinations,but lower from the（combination） of Glycyrrhiza uralensis Fisch and Bupleurum chinense DC（P﹤0.05）.Conclusion The decocting quantity of liquiritigen and isoliquiritigenin from the different formula combinations of Sini（Powder） has a significant difference.关键词：Sini Powder;combinations;liquiritigen;isoliquiritigenin;RP-HPLC;content determination
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发布时间：2021-08-10 - 摘要：Objective To study and establish the fingerprint chromatogram of raw Herba Selaginellae by using the reversed phased-high performance liquid chromatography（RP-HPLC）,and to supply a reliable base for evaluating and controlling the quality of Herba Selaginellae.Method In RPHPLC procedure Zorbax C18（4.6 mm×150 mm,5 μm） column was used with mixture solution of methanol and-0.1% formic acid aqueous as mobile phase in a gradient mode.The flow rate was 1.0 mL/ min and the（detection） wave length was set at 330 nm.Result A standard was set up for the detection of fingerprint chromatogram of raw Herba Selaginellae.Conclusion This method is simple,accurate with a good（repeatability）,and provides a base for the quality control standard of Herba Selaginellae.关键词：Herba Selaginellae;RP-HPLC;fingerprint chromatogram
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发布时间：2021-08-10 - 摘要：Objective To study the method for controlling the uniformity of raw powder preparation of Chinese herbal drugs taking Angongniuhuang Pills as the object.Methods The near infrared spectrum（NIRS） were collected from different points of raw materials and analyzed with similarity calculation.The relationship between the similarity and uniformity was compared.Result The uniformity of raw materials can be reflected by the similarity of NIRS at different points.Conclusion This method can be used for controlling the uniformity of raw materials of Angongniuhuang Pills,which provides a fundamental for（uniformity） measure and quality control of raw powder products of Chinese raw herbal materials or Chinese patent medicines.关键词：near infrared spectrum;uniformity;Angongniuhuang Pills;quality control
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发布时间：2021-08-10 - 摘要：Objective To study the characteristics of TCM syndrome manifestations of metabolic syndrome on the base of type 2 diabetes mellitus.Method The retrospective materials of 254 hospitalized cases were chosen from the Department of Chinese Medicine,Beijing Hospital and the TCM syndrome characteristics of metabolic syndrome were discussed from the aspects of sex,age and disease course,taking the world definition of IDF’s metabolic syndrome in 2005 as the standard.Result The（manifestations） of syndrome of both qi and yin deficiency and syndrome of blood stasis in meridians had a superiority in the number,but no statistic correlation with metabolic syndrome,while syndrome of dampnessretention due to spleen insufficiency showed the statistic correlation in male,adult patients with metabolic syndrome in middle stage of disease course.Conclusion The syndrome of both qi and yin deficiency is main syndrome type of metabolic syndrome of type 2 diabetes mellitus.Some syndrome manifestations are related to age,sex and disease course,which indicates that "dampness-retention due to spleen insufficiency" is one of the main manifestations of metabolic syndrome of type 2 diabetes mellitus.关键词：type 2 diabetes mellitus;metabolic syndrome;Chinese medicine;syndrome manifestations
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发布时间：2021-08-10 - 摘要：Objective To observe the curative effect of Bushentongluoshugan Formula on partial（hypoandrogenism）（PH） in middle-aged and aged males.Methods The questionnaire score and serum total testosterone（TT） determination were used to screen 69 patients with PH（mean age was 61.77,without organic diseases） from andrology OPD from Feb.2002 to Oct.2005.The patients were divided into the treatment group（n=36） given Bushentongluoshugan Formula orally for 3 months,and the（control） group（n=33） not given any special treatment.The changes of PH scores and sexual hormone levels were observed and compared in two groups before and after the treatment.Result The total PH score,structure score and TT level in the treatment group were improved obviously after 3 month（treatment）（P<0.05） with a significant difference compared with the control group（P<0.05）.The（result） in the control group was not changed（P>0.05）.Conclusion Bushentongluoshugan Formula is safe and effective for treating PH in middle-aged and aged males.关键词：Bushentongluoshugan Formula;middle-aged and aged males;hypoandrogenism
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发布时间：2021-08-10 - 摘要：Objective To observe the curative effect of arterial perfusion of some Chinese herbal drugs（injection of Kanglaite,elemene and cinobufagin） on metastatic liver cancer.Method The patients with metastatic liver cancer（n=120） were randomly divided into the treatment group（n=52,treated with arterial perfusion of Chinese herbal drugs） and the control group（n=50,treated with chemical medicines）.Result The effective rate was 25.0% and the rate of clinical benefit was 73.1% in the treatment group,which was not different compared with those of the control group.The survival rate of 1 year and 2 years was respectively 67.3% and 32.6%,which was obviously higher than that of the（control） group.Conclusion The arterial perfusion of Chinese herbal drugs could protect the liver function of the（patient）,promote cell immunity,improve patient’s life quality and had no obvious toxic and side effects.The therapy of arterial perfusion of Chinese herbal drugs has a reliable curative effect,which（provides） new ways for treating malignant tumors with Chinese medicine.关键词：Chinese herbal drugs;arterial perfusion;metastatic liver cancer
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发布时间：2021-08-10 -
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