最新刊期
卷 0 ， 期 0 ， 2020
- 摘要：Objective To combine diagnosis of Chinese medicine with complicated algorithm to study a new way for syndrome diagnosis by complicated algorithm. Methods Through multicentre epidemiologic survey 696 cases including 5 kinds of diseases were colected. Correlation rate of information entropy and correspondence analysis were used to investigate syndrome of blood stasis and its subtypes. Results The symptoms and signs of 4 subtypes of syndrome of blood stasis, deficiency of qi and blood stasis, qi- stagnancy and blood stasis, yin asthenia and blood stasis and phlegm-turbid and blood stasis, were got out based on correlation rate of information entropy, and 12 common symptoms and signs shared by 4 subtypes were taken as symptoms and signs of total syndrome of blood stasis. The patients with syndrome of qi-stagnancy and blood stasis contributed more to total syndrome of blood stasis than those with other 3 subtypes. Correspondence analysis was applied to get the table of correlated distance between disease and subtype of syndrome of blood stasis. The results showed that syndrome of deficiency of qi was located at the centre of 5 syndromes of blood stasis with different situs. Rheumatoid arthritis （RA）, hepatic cirrhosis, irregular menses, cerbral infarction and angina pectoris had most close relationship with yin asthenia and blood stasis, qi-stagnancy and blood stasis, qi-stagnancy and blood stasis and deficiency of qi and blood stasis, phlegm-turbid and blood stasis and qi-stagnancy and blood stasis, respectively. Conclusion Complicated algorithm is applicable to study the normalization of syndrome of Chinese medicine. Syndrome of deficiency of qi is the main syndrome accompanied with blood stasis and different subtypes of syndrome of blood stasis have their special symptoms and signs apart from common symptoms and signs.关键词：syndrome of blood stasis;correlation rate of information entropy;correspondence analysis
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发布时间：2021-09-15 -
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- 摘要：Objective To investigate the pathogenesis of hyperuricacidemia in quail model by observing the changes of various kinds of enzyme activity in different pathological stages. Methods Biochemical and molecular biological methods were applied to dynamically observe activity and gene expression of xanthine oxidase （XO）, Guanosine deaminase （GD）, adenosine deaminase （ADA）, lipoproteinesterase （LPL） and hepatic lipoidase （HL） in serum and tissue. Results In the model group, XO activity and gene expression in the liver increased, while ADA and LPL activity decreaced significantly. GD activity had no significant change and HL activity changed alternatively. Conclusion The pathogenesis of hyperuricacidemia in quail model may be associated with increased XO activity and reduced LPL activity. The relationship between the activity of ADA and HL and pathological process of hyperuricacidemia needs to be further investigated.关键词：hyperuricacidemia;model;pathogenesis;Quail
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发布时间：2021-09-15 - 摘要：Objective To evaluate and optimize the clinical symptom information collection table adopted in syndrome study of chronic renal failure by the method of reliability estimation to establish a reliable and effective symptom information collection table. Methods The investigate was carried out in 6 centers of entaire country and out-patients and in-patients who visited hospital continuouslly were chosen. Two trained qualified doctors collected symptom information from a patient at the same time. The coefficient correlation of scores to same symptom from two doctors was calculated. Results Score differences of diffrernt inter-raters could be reflected by reffering to the method of inter-raters reliability estimation to evaluate the information collection table. The coefficient correlations of most symptoms in information collection table corresponded to the requirement. Conclusion This method can be used to evaluate information collection table. The symptom information collection table of chronic renal failure can be used to investigate symptoms in syndrome study.关键词：chronic renal failure;symptom information collection table;evaluation
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发布时间：2021-09-15 - 摘要：Objective To analyze syndrome distribution of perimenopausal period syndrome （PPS） and explore syndrome differentiation method by clinical epidemiology method. Methods Every patient was differentially diagnosed by TCM symptom weighted score method. Results Among 340 patients, 156 patients were differentially diagnosed as the single syndrome, including kidney yin deficiency, kidney yang deficiency, deficiency of both kidney yin and yang, disharmony between heart and kidney, stagnation of liver-qi and deficiency of both heart and spleen. One hundred and eighty-four patients were diagnosed one or compound syndromes of those six. The number of patients with above six syndromes was 49, 20, 105, 49, 25 and 47, respectively. Conclusion TCM symptom weighted score method settles the following problems: quantization, classification and the weight of clinical symptoms and sign, the combination of primary and secondary clinical symptoms and signs, and the TCM syndrome diagnosis criteria.关键词：perimenopausal period syndrome;syndrome;TCM symptom weighted score method
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发布时间：2021-09-15 - 摘要：Objective To establish a literature database of common syndrome manifestations, symptomes, signs and syndrome factors of chronic viral hepatitis B. Methods Access 2003 database software was used to establish literature database based on the syndrome manifestations, symptomes and signs of chronic viral hepatitis B searched from mordern periodicals by handwork or cmputer, and syndrome factors in documents were got out. Results The literature database of chronic viral hepatitis B was established and analysis of syndrome manifestations, symptomes, signs and syndrome factors was completed. Conclusion It is convenient and efficient to use Access 2003 database software to process literature data in which number of variates is unknown.关键词：chronic viral hepatitis B;Chinese medicine;literature;database;Access
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发布时间：2021-09-15 - 摘要：Objective To screen gene fragment differentially expressed in testicle in winter and summer. Method Differential display reverse transcription PCR （DDRT-PCR） was used to detect the total RNA in testicle sample. Results ①Forty-four gene fragments differentially expressed were obtained. Ten gene fragments sequence were detected and 7 of them had higher homology with known genes. ②Three new genes were discovered and were included by gene bank （CK743226,CK743227,CK743228）. Conclusion ①In physiological situation, seasonal variation of winter and summer can result in change of gene expression in testical. ②Conarium regulats gonad according to season by differential expression of gene. ③Three new genes may be needed by animals for self-regulation according to season and for evolvement . ④The theory of “kidney corresponding with winter” has substance basis. Its regulating mechanism is related to conarium’s regulation to differential expression of gene in testical.关键词：kidney corresponding with winter;conarium;testicle;differential expression of gene;rats
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发布时间：2021-09-15 - 摘要：Objective To investigate the effect of fatty on insulin resistance in high fat-fed rats. Methods Thirty male Wistar rats including the normal group and the high fat-fed group were used in study. The control rats were fed with common diet.The high fat-fed group were fed with high fat diet containing saturated free fatty acids to provide 59% caloric. All of items were detected after each group was fed for 12 weeks. Results Compared with the normal group, the body weight from week 4 to the end, fasting blood glucose and blood glucose after 30,60 and 120 min of oral glucose, the tolerance test area under the glucose curve, blood glucose after 40,90 and 120 min of insulin injection subcutaneously and the area under glucose curve in the insulin tolerance, serum insulin, insulin resistance index, serum leptin, serum cholesterol, serum triglyceride and saturated fatty acid were significantly increased in the high fat-fed group, while the unsaturated fatty acid was significantly decreased. Conclusion The substance basis of insulin resistance induced by fatty is related to abnormal lipid metabolism, higher saturated fatty acid and lower unsaturated free fatty acids.关键词：fatty;insulin resistance;substance basis
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发布时间：2021-09-15 - 摘要：Objective To study the contents and determination method of cryptotanshinone,tanshinone I and tanshinone IIA in Guanxindanshen Capsules and Guanxindanshen Gutta Pills. Methods Ultrasonic extraction was used to extract the fat-soluble components of Guanxindanshen Capsules and Guanxindanshen Gutta Pills as samples and RP-HPLC assay was established to determine cryptotanshinone, tanshinone I and tanshinone IIA in these samples. The HPLC column was Diamonsil TM C 18（250 mm×4.6 mm,5 μm）. The mobile phase was methanol-water（75∶25）with the flow rate of 0.8 mL/min and the detection wavelength was 270 nm. Results The calibration curves of cryptotanshinone,tanshinone I and tanshinone IIA were linear in the range of 1.456 to 10.190 mg/L,2.160 to 15.120 mg/L and 3.152 to 22.060 mg/L with the correlation coefficient beyond 0.999 1,0.999 2 and 0.999 6, respectively.The mean recoveries of the assay were 98.0% （RSD=1.30%,n=3） for cryptotanshinone,100.0% （RSD=0.96%,n=3） for tanshinone I and 99.3% （RSD=1.40%,n=3） for tanshinone IIA, respectively. Conclusion The method is rapid ,accurate and reliable, and can be used to control the quality of Guanxindanshen Capsules and Guanxindanshen Gutta Pills.关键词：Guanxindanshen Capsules;Guanxindanshen Gutta Pills;cryptotanshinone;tanshinone I;tanshinone IIA;RP-HPLC
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发布时间：2021-09-15 - 摘要：Objective To confirm the serum pharmacochemistry of Xingnao Gutta Pills. Methods Analysis method of HPLC fingerprint for Xingnao Gutta Pills and rat serum with Xingnao Gutta Pills was established. HPLC fingerprints of Xingnao Gutta Pills and rat serum with or without Xingnao Gutta Pills were compared and the fingerprint peaks were confirmed by addition of control article. Results In serum with Xingnao Gutta Pills, 5 compounds were not absorbed or had been metabolized. 18 compounds were detected, 10 of them were original compounds, 8 of them were metabolits. Conclusion Serum pharmacochemistry of Xingnao Gutta Pills is confirmed initially, which establishes a basis for further study on active compound in Xingnao Gutta Pills and mechanism.关键词：Xingnao Gutta Pills;HPLC fingerprint;serum pharmacochemistry;rats
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发布时间：2021-09-15 - 摘要：Objective To investigate the identification of cornu cervi pantotrichum in Guiyuan Oral Solution （GOS） and to provide the scientific basis for the qualitative standard control. Methods TLC was used for qualitative identification and HPLC was used for quantitative detection. Results In TLC procedure the control material and the test sample showed the same coloured spots of red brown, while the negative control sample showed no interference. In HPLC procedure the contents of uridine and hypoxantine were determined in cornu cervi pantotrichum. The chromatographic column was ODS2 C 18, the mobile phase was[methanol to tetrahydrofuran （2∶1）]-water（0.1∶99.9）, and the detection wavelength was 254nm. The average recovery of uridine was 99.67% （RSD=2.23%） and that of hypoxantine was 98.18% （RSD=2.14%）. Conclusion TLC-HPLC can be used as one of the methods in the qualitative control of cornu cervi pantotrichum in GOS. When the dosage of cornu cervi pantotrichum was more than 5.0% in GOS the lowest concentration of uridine and hypoxantine in the test sample would be higher than 0.130 g/L and 0.050 g/L respectively.关键词：TLC;HPLC;Guiyuan Oral Solution;Cornu cervi pantotrichum
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发布时间：2021-09-15 - 摘要：Objective To optimize the conditions of hydrolysis processing of black-bone chicken by using pepsin, tripsin, papain, neutrase and fiberase. Method The conditions of temperature, duration, pH and the ratio of enzyme to substrate were compared by using the absorbance of amino acids in enzymolysis liquid as indicator. Orthogonal test was used to optimize the conditions of hydrolysis. The efficacy of the hydrolysis product on replenishing qi and nourishing blood and regulating manstruation and stoping leukorrhagia was validated by comparing with that of Wujibaifeng Pills. Result Hydrolysis conditions were pH 8.3, neutrase 12 000 U, temperature 50 ℃ and duration 4 h. Conclusion The pharmacological efficacy of Wujibaifeng Pills produced by new processing is same as that of the old one.关键词：black-bone chicken;enzyme;enzymatic hydrolysis processing;Wujibaifeng Pills
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发布时间：2021-09-15 - 摘要：Objective To investigate proper application of statistics on curative effect evaluation of disease and syndrome of TCM.Methods Kruskal-Wallis rank test,Ridit analysis,univariateANOVA and MANOVA were used to analysis the data gained according to the project of treating and preventing diabetic nephropathy renal inadequacy. Results The curative effects and total syndrome integral of both the chinese herbs treated group and the integrated Chinese and western medicine treated group were better than that of the western medicine treated group.The chinese herbs treated group had better results in general curative effect,serum Cr reducing,CCr enhancing and renal function improving than the other tow groups. There was no notable difference between the integrated Chinese and western medicine treated group and the western medicine treated group in the above-mentioned aspects. Conclusion Treatment according to syndrome differentiation is the optimized therapy scheme for diabetic nephropathy renal inadequacy. Applying methods of statistics properly is the key link in the study on curative effect evaluation of disease and syndromes of TCM.
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发布时间：2021-09-15 - 摘要：Objective To study the effect of Kaiyuqingwei Granules on the beta-cell function in patients with different blood glucose levels,and discuss the relationship of different plasma glucose levels,blood glucose and beta-cell function. Methods Subjects were divided into three groups according to 2 h postprandial blood glucose（PBG） levels of oral glucose tolerance test（OGTT） as follows:Group 1,7.8 mmol/L≤PBG<11 .1 mmol/L; Group 2,11.1 mmol/L≤PBG<16.7 mmol/L; Group 3,PBG>16.7 mmol/L. Sixty-two patients with abnormal glycometabolism were observed before and after treatment by Kaiyuqingwei Granules and OGTT data was used to calculate Homa β-cell function index（HBCI）,the ratio of insulin increment to PBG increment 30 min after glucose load（ΔI30/ΔG30）and insulin area under curve （INSAUC） to evaluate beta-cell function.Results ①In Group 1 and Group 2, after treatment by Kaiyuqingwei Granules,the body weight, blood glucose and blood fat of patients were improved. Insulin sensibility index（ISI） and HBCI、ΔI30/ΔG30 increased,while INSAUC and insulin at different time decreased. ②In Group 3, blood glucose and blood fat of patients decreased and ISI increased after treatment. HBCI and ΔI30/ΔG30 increased a little,while INSAUC and insulin of different time point had no change. Conclusion When PBG<16.7 mmol/L, Kaiyuqingwei Granules improve β-cell fanction by decreasing blood glucose and blood fat and increasing insulin sensibility. The improvement of the early-phase insulin secretion is closely associated with the significant decrease of blood glucose 2 h after glucose load.When PBG>16.7 mmol/L,high-level glucose restrains insulin secretion of beta-cells, which indicates that glucose toxicity does a lot of damage to beta-cell function. Kaiyuqingwei Granules improve beta-cell function by decreasing blood glucose and attenuate glucose toxicity.关键词：Kaiyuqingwei Granules;glucose;blood glucose;beta-cell function
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发布时间：2021-09-15 - 摘要：Objective To observe the effect of Huatanquyu Decoction （HD） on neurofunctional impairment score,tissue type plasminogen activator （t-PA）,tissue type plasminogen activator inhibitor （PAI） and on traditional Chinese medicine （TCM） syndrome in patients with acute cerebral infarct. Methods Forty-two patients with acute cerebral infarct were randomly divided into two groups. The control group （n=22） were treated with the conventional treatment. On the basis of conventional treatment, the HD was given to the treated group （n=20） additionally. The neurofunctional impairment score, TCM syndrome grade, t-PA and PAI were determined before and after treatment. Results There was a significant difference in the total effective rate of TCM syndrome score between the treated group（75%） and the control group （41%） （P<0.05）. There was a significant improvement in the neurofunctional impairment scores,t-PA and PAI（P<0.05）. Conclusion HD has the significant effects of improving neurofunctional impairment score,increasing t-PA and decreasing PAI. HD markedly improves the therapeutic effects when combined with the conventional treatment in the patients with acute cerebral infarct. The mechanism could be related mainly to its effects on t-PA and PAI activation.
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发布时间：2021-09-15 - 摘要：Objective To explore the endocrine mechanism of promoting labor through electroacupuncture at Hegu（LI4） and Sanyinjiao（SP6） points with different duration of needle retention in late pregnant rats. Methods A total of 60 Wistar rats were randomized into 4 electroacupuncture groups, normal control group and pregnant control group with 10 cases in each group according to the requirements of 2×2 factorial experiment. The contents of prostaglandin E2 （PGE2）, estradiol （E2） and progesterone （P） in serum were detected. Results The duration of needle retention in both LI4 and SP6 after needling LI4 firstly and SP6 afterward had more important influence on the contents of PGE2. The level of retention needles for 5 min after needling SP6 had better effect in increasing the PGE2 contents than the level of 20 min as well as the value of E2/P. The duration of needle retention of 20 min or 40 min at LI4 had significant influence on P contents only. Needle retention of 40 min could significantly increase P contents than that of 20 min. Only the combination of needling LI4 20 min firstly and SP6 5 min afterward could significantly increase the contents of PGE2, E2 and P in serum and the value of E2/P. Conclusion Electroacupuncture at LI4 and SP6 with different time factors has different effect on promoting labor. Its mechanism may be synergy or antagonism to the effect of above two points on promoting labor by way of endocrine and nerve reflex.关键词：electroacupuncture;duration of needle retention;acupuncture-moxibustion effect;Hegu（LI4）;Sanyinjiao（SP6）;factorial experiment
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发布时间：2021-09-15
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