最新刊期
卷 0 ， 期 0 ， 2020
- 摘要：Objective To explore the modern methodology on the study of TCM syndrome manifestation.Method The methods of mathematical statistics used in TCM syndrome manifestation study were collected from the magazines of TCM of recent years.Results At the present time,the methods of quantification and mathematical statistics used in the study of TCM syndrome manifestation mainly are questionnaires for experts,multiple factor regression analysis,maximum likelihood distinguish method,Logistic regression,factor analysis,principal component analysis,canonical correlation analysis, structural equation model,etc.Conclusion At the present stage,the ideal method in the study of TCM syndrome manifestation is to begin with clinical study according to the aim of study,then to use several statistical methods together after some results have been obtained from clinical study.关键词：TCM syndrome manifestation;quantification;statistical method
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发布时间：2021-09-15 -
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- 摘要：Objective To study the regulatory mechanism of "kidney corresponding with winter" through the observation of the seasonal changes of melatonin receptor.Method The radioligand saturation method was used to detect the changes of maximal combination（Bmax） value and dissociation constant（Kd value） of melatonin receptor of sex gland axis in winter and in summer in male SD rats.Results Bmax values of melatonin receptor of hypothalamus and pituitary in the normal group were both high in winter and low in summer（P<0.05,P<0.05）,while Kd values of melatonin receptor of them were also high in winter and low in summer（P<0.05,P<0.01）.In the pineal gland removed group,Bmax values of melatonin receptor of hypothalamus and pituitary were higher than those of the normal group in winter（P<0.01,P<0.01）,and Bmax value of melatonin receptor of hypothalamus was higher than that of the normal group in summer（P<0.01）.Kd values of melatonin receptor of hypothalamus and pituitary in the pineal gland removed group were higher than those in the normal group in winter and in summer（P<0.01,P<0.01）.Bmax value and Kd value of melatonin receptor could not be detected in testicle.Conclusion The regulatory mechanism of "kidney corresponding with winter" is related with the seasonal changes of melatonin receptor,and the pineal gland plays an important role in the process of regulation.关键词：kidney corresponding with winter;sex gland axis;melatonin recepter;rats
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发布时间：2021-09-15 - 摘要：Objective To study the effect of Weimaining（WMN） on adhesion between high metastasis lung cancer cell line（PG） and human umbilical vein endothelial cell（HUVEC） in vitro to investigate the mechanism of anti-tumor metastasis of WMN.Methods The effects of WMN on the vitality of PG and HUVEC were detected by MTT.The effect of WMN on adhesion of PG to HUVEC and on adhesion of TNF-activated HUVEC to PG were investigated by Rose Bengal staining.Results WMN（31.25、62.5 mg/L） had no effect on PG and HUVEC vitality after treating cells for 24 h（P>0.05）.WMN（31.25、62.5 mg/L）obviously decreased PG-HUVEC adhesion after treating PG for 24 h（P<0.05,P<0.01）.HUVEC-PG adhesion decreased significantly after TNF-induced HUVEC was treated by WMN for 6 h and 12 h（P<0.01）.Conclusion WMN may inhibit the adhesion between PG and HUVEC by effect on both PG and HUVEC.Thereby,WMN can inhibit blood route metastasis of tumor cells.关键词：Weimaining;PG;human umbilical vein endothelial cell（HUVEC）;adhesion
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发布时间：2021-09-15 - 摘要：ObjectiveTo study the influence of various concentrations of serum with Naoluoxintong on growth and differentiation of rat embryonic neural stem cells.Methods The growth and differentiation of rat embryonic neural stem cells cultured in various concentrations（0,2.5%,5%,and 10%） of serum with Naoluoxintong were observed.The phenotypic changes of neural stem cells were detected under the phase contrast microscopy and specific markers of neurocytes（neuron,astrocyte and oligodendrocyte） were detected by immunohistochemistry.Results Various concentrations of serum with Naoluoxintong could promote the growth of neural stem cells to some extent,and the 10% serum with Naoluoxintong had the most effective performance. All concentrations of serum with Naoluoxintong could induce the majority of neural stem cells to differentiate into neurons,astrocytes or oligodendrocytes.The differentiated ratio of neurons was the highest in 10% serum with Naoluoxintong,which consequently made the differentiated neurons resemble mature neurons morphologically to greater extent.Conclusion Naoluoxintong can promote the growth and differentiation of cultured rat embryonic neural stem cells.Different concentrations of serum with Naoluoxintong have different effect on growth and differentiation of neural stem cells,and the effect of 10% serum is optimum.关键词：neural stem cells;growth;differentiation;Naoluoxintong;drug serum;rats
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发布时间：2021-09-15 - 摘要：ObjectiveTo observe the effects of main components of pearl powder,dragon’s blood,boswellin,velver antler and arsenolite ore,including soluble pearl powder（SPD）,dracohodin perochlorate（DP）,boswellin aqueous extract（BAE）,velver antler polypeptide（VAP） and arsenic trioxide（AT）,on the proliferation and collagen synthesis of human fibroblasts（HFB） to investigate the therapeutic mechanism on chronic ulcer of skin.Methods We obtained populations of normal human fibroblasts from primary tissue mass culture in vitro.MTT assay was used for observing cell proliferation,and hydroxyproline chromatometry was used for detecting collagen synthesis.Results After HFB was cultured with the tested drugs for 48 hours,the proliferation rate and collagen synthesis of HFB were elevated by SPD,DP,BAE,VAP and lowered by AT.For SPD,DP,BAE and VAP,obvious proliferation-promoting concentrations on HFB were 1.25×1021×103 mg/L,5.0×1002.0×101 mg/L,6.4×10-12.0×103 mg/L and 6.25×10-34.0×10-1 mg/L,respectively（P<0.01,P<0.05）.In concentrations of 1.6×10-18.0×10-1 mg/L,AT lowered the proliferation rate of HFB.After treating HFB for 48 hours,SPD,DP,BAE and VAP could stimulate collagen synthesis in concentrations of 2.5×1021.0×103 mg/L,2.5×100～2.0×101 mg/L,6.4×10-1～2.0×103 mg/L and 1.6×10-34.0×10-1 mg/L,respectively（P<0.05 or P<0.01）,and AT could decrease collagen synthesis in concentrations of 3.2×10-28.0×10-1 mg/L.Conclusion SPD,DP,BAE and VAP can elevate the proliferating rate and collagen synthesis of HFB.On the contrary,AT can decrease the proliferation rate and collagen synthesis of HFB.关键词：proliferation;collagen synthesis;fibroblast;topically used traditional Chinese medicine
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发布时间：2021-09-15 - 摘要：objectiveTo investigate the mechanism of protective effect of Qixuanyixin Capsules on diabetic rats with myocardial ischemia.Methods The diabetic rat models with myocardial ischemia were reproduced by injecting alloxan intraperitoneally to cause pancreas damage and injecting pituitrin in ranine vein to produce myocardial ischemia,which were randomly divided into Minidiab group,Danshen Gutta Pills group,high dose of Qixuanyixin Capsules group,medium dose of Qixuanyixin Capsules group,low dose of Qixuanyixin Capsules group and model group.There was a blank control group with the same number of healthy Wistar rats.The expression of NF-κBp50,IκBα and IL-1β portein and mRNA of acute ischemic cardiac muscle in rats of all groups were detected.Results Compared with the blank control group,the expression of NF-κBp50,IκBαand IL-1β portein and mRNA of acute ischemic cardiac muscle were higher in the other groups（P<0.05）.In high,middle and low dose of Qixuanyixin Capsules groups,the levels of NF-κBp50,IκBαand IL-1β protein and mRNA were lower than in the model group（P<0.05）,and the effect of Qixuanyixin Capsules was better than that of Danshen Gutta Pills and Minidiab（P<0.05）.Conclusions The activation of NF-κBp50 and IL-1β lies in ischemic cardiac muscle of diabetic rats,which can be inhibited by Qixuanyixin Capsules to some degree.It is one of the important protective mechanisms of Qixuanyixin Capsules on cardiac muscle.关键词：Qixuanyixin Capsules;diabetes;acute ischemic cardiac muscle;animal model;rats.
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发布时间：2021-09-15 - 摘要：Objective To study the effect of equisetum n-butanol extract on the apoptosis and Fas/FasL expression of arterial smooth muscle cell（ASMC） in rats with early atherosclerosis（AS）.Method Fourty SD rats were divided into four groups: the normal control group,the model group,the gemfibrozil treating group and the equisetum extract treating group.The early AS model was reproduced by high fat diet.The model rats were administered with equisetum extract prophylactically,while gemfibrozil was used as positive control drug.After nine weeks,the rate of apoptosis and the Fas/FasL expression of ASMC were detected by the flow cytometry,and the morphological changes of aorta were observed under the light microscopy.Results The rate of apoptosis and the Fas/FasL expression of ASMCs in the equisetum treating group were both higher than those of the model group（P< 0.01,P<0.05）,and the degree of atherosclerotic lesion was less than that of the model group.Conclusion Equisetum extract can regulate the expression of Fas/FasL and promote the apoptosis of ASMC of early AS to prevent the progression of early atherosclerosis.关键词：Equisetum extract;atherosclerosis;smooth muscle cell;apoptosis
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发布时间：2021-09-15 - 摘要：Objective To examine the therapeutic effect of Jiaweibiejiajian（JWBJ） Pills on rats with hepatic fibrosis induced by carbon tetrachloride（CCl4）.Method Wistar rats model with hepatic fibrosis was induced by the multiple subcutaneous injections of 40% CCl4.The first dose was 5 mL/kg,and then 3 mL/kg,twice a week for 6 weeks.When hepatic fibrosis was verified to come into being by pathological examination,the rats were divided into model-group,Colchicine group;Ganpikang group;low dose of JWBJ group,medium dose of JWBJ group and high dose of JWBJ group.JWBJ was administered orally for 8 weeks.Aspartate aminotranferase（AST）,alanini transaminase（ALT）,total protein（TP）,albumin（ALB） hyaluronic acid（HA）,laminin（LN）,collagen Ⅳ（CⅣ） in serum of all rats were examined.Results Compared with the model-group,ALT,AST,HA,LN and CⅣ decreased obviously,ALB,TP and the ratio of A/G increased obviously in the medium dose and highdose of JWBJ group.Conclusion JWBJ has better therapeutical effect on rats with hepatic fibrosis induced by CCl4.关键词：Jiaweibiejiajian pills;hepatic fibrosis;carbon tetrachloride（CCl4）;rats
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发布时间：2021-09-15 - 摘要：objective to investigate the effects and mechanism of diversifolious patrinia root（DPR） extract on proliferation and apoptosis of K562 cells to offer experimental data for screening bioactive components from DPR.Methods MTT method was used to detect inhibition ratio to K562 proliferation and IC50.The morphological characteristics of apoptosis cells were observed by trypan blue stain.The apoptosis ratio and cell cycle of K562 were measured by flow cytometry（FCM）.The expression of apoptosis related protein bcl-2 and bax were determined by immunocytochemistry.Results DRP extract obviously inhibited the proliferation of K562 cells in a does-dependent manner in the definite dose range,and the IC50 was 36.03 mg/L.The typical morphological characteristics of apoptosis cells could be seen under the inverted microscope.The apoptosis rates in the treatment groups were significant higher than that in the control group（p<0.05）.The expression of bcl-2 protein was down-regulated,and the expression of bax protein was up-regulated after K562 cells were treated by DRP extract for 48 h,and the differences between the treatment group and the control group were significant（p<0.05）.Conclusion DRP extract can inhibit the proliferation and induce the apoptosis of K562 cells,which may be one of the mechanisms of anti-tumor effect of it.关键词：diversifolious patrinia root;K562 cells;apoptosis;apoptosis related protein
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发布时间：2021-09-15 - 摘要：Object To establish a method for the quantitative determination of ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside R1 in the effective fraction of Qingnaoxuanqiao Formula by HPLC.Method The determination was carried out with phenomenex Luna NH2 column（250 mm× 4.6 mm,5 μm）.CH3CN-H2O（80∶20） was taken as mobile phase at a flow rate of 1.0 mL/min,and detection wavelength was 203 nm.Result The average recoveries of ginsenoside Rg1,ginsenosideRb1 and notoginsenoside R1 were 101.86%,102.02% and 101.23% respectively,and RSD s of the above three compounds were 1.68%（n=9）,1.45%（n=9） and 1.28 %（n=9） respectively.Conclusion The method is simple and accurate,and can be used to determine the content of ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside R1 in the effective fraction of Qingnaoxuanqiao Formula.
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发布时间：2021-09-15 - 摘要：Objective To establish a HPLC method for quantitatively determining cimicifugoside and 5-o-methylvisammioside in Raidix Saposhnikoviae of Xiaoerzhiyang Capsules.Method The mobile phase was methanol-water（40∶60） and the detection wavelength was 254 nm.Results There was a good linear relationship for cimicifugoside from 0.388 8 μg to 0.907 2 μg（r=0.999 9） and for 5-o-methylvisammioside from 0.127 2 μg to 0.763 2 μg（r=0.999 6）.The average recovery of the procedure was 101.4%（RSD=1.62%）.Conclusion The method is accurate and easy to be performed.关键词：Xiaoerzhiyang Capsules;cimicifugoside;5-o-methylvisammios-De;high performance liquid chromatography;quantitative determination
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发布时间：2021-09-15 - 摘要：Objective To systematically study the chemical components of the effective fraction of Acorus tatarinowii Schott.Method The chromatography with silica gel,thin-layer and pharmadex LH-20 were used to isolate the compositions of the ethanol extract from rhizomes of Acorus tatarinowii Schott and the spectrum was used to identify the structure of the compositions.Result Eight compounds were isolated and indentified as follows: β-asarone（Ⅰ）,α-asarone（Ⅱ）,β-sitosterol（Ⅲ）,stigmasterol（Ⅳ）,galgravin（Ⅴ）,veraguensin（Ⅵ）,2,4,5,-trimethoxybenzoic acid（Ⅶ） and eudesmin（Ⅷ）.Conclusion CompoundsⅤ,Ⅵ and Ⅷ are isolated from Acorus calamus L.genus for the first time and it is the first time to get compositions of helices and bisepoxy lignans from Acorus calamus L.genus.关键词：Acorus tatarinowii Schott;chemical composition;lignanoid poxylignans
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发布时间：2021-09-15 - 摘要：Objective To observe the therapeutical effect of autologous bone marrow mononuclear cell injection along the meridians according to syndrome differentiation on ischemia in artery of lower extremity.Method Forty-two patients with ischemia in artery of lower extremity were given the formula with Chinese medicine of replenishing qi,activating blood and promoting blood circulation for 1 week.Then autologous bone marrow mononuclear cells got from patient by density gradient centrifugation were injected along the meridians in ischemic extremity of patient according to syndrome differentiation.The patients were given Chinese medicine of warming yang,warming meridians and unblocking collaterals on the basis of the previous formula for 4 to 6 weeks after injection.Before and after treatment,ankle/brachial index（ABI）,photoplethysmography（PPG）,transcutaneous arterial oxygen tension（TcPO2）,transcutaneous carbon dioxide tension（TcPCO2）,painful feeling,cold feeling,growth and healing of raw surface were observed and then the curative effect was evaluated synthetically.Results The clinical symptoms of all patients were improved to different degree 1 to 2 weeks after injection and rest pain and cold feeling were improved more obviously.Two weeks after injection,TcPO2 increased and TcPCO2 decreased to some degree at 10 cm below articular genu.Four weeks after injection,there was tendency of increase in ABI, and the wave form of PPG was improved,and the limping distance was prolonged,and the healing cycle was shortened.Conclusion Autologous bone marrow mononuclear cell injection along the meridian according to syndrome differentiation has significant effect on improving clinical symptoms and signs of patients with ischemia in artery of lower extremity.关键词：syndrome differentiation;injection along the meridian;bone marrow mononuclear cell;ischemia of lower extremity
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发布时间：2021-09-15 - 摘要：objective To observe the curative effect of treating postinfectious cough using eliminating wind and releasing lung medicines,and to investigate mechanism of Chinese Medicine curing postinfectious cough.Method 60 patients with postinfectious cough were divided into 2 groups using partnership design:40 patients in the Chinese Medicine group and Eestern medicine group,some symptom,like cough,itch of pharynx,curative effect and course of diseases,were observed and compared in 2 groups.Result The result of the Chinese Medicine treatment group was superior to that of the Western Medicine control groups with a statistical significance in the curative effect.Conclusion The effect of eliminating wind and releasing lung medicines curing postinfectious cough is very good,some fators,like easing inflammation of respiratory tract,repairing disruption of airway epithelial integrity and depressing airway responsiveness are likely to the mechanism.关键词：postinfectious cough;wind invasion of lung;the method of eliminating wind and releasing lung
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发布时间：2021-09-15 - 摘要：Objective To observe the influence of acupuncture onβ-EP levels of plasma,hypothalamus,pituitary and lymph node in rats with adjuvant-induced arthritis.Methods The adjuvant arthritis model rats were given electro-acupuncture on the "six points of conception vessel（CV）",tanzhong（CV17）,yutang（CV18）,zigong（CV19）,huagai（CV20） and xuanji（CV21）,and on the acupoint of guanyuan（CV4）.Radio-immunity was used to determine the β-EP levels in serum,hypothalamus,pituitary and lymph node.Results The acupuncture could regulate theβ-EP levels in different tissues.After the acupuncture the β-EP level in hypothalamus showed depressed tendency,while in plasma,pituitary and lymph node,presented elevated tendency.Conclusion The acupuncture can inhibit the inflammatory reaction and regulate the β-EP levels of different tissues and organs to lighten the damage caused by harmful stimulation.关键词：acupuncture;adjuvant arthritis;β-EP
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发布时间：2021-09-15 -
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