最新刊期
卷 0 ， 期 0 ， 2020
- 摘要：Objective The foundational theory of Chinese medicine is the core contents of Chinese medicine. It is the demand of the development of not only the discipline itself but also the history that the development of the foundational theory promotes the development of Chinese medicine. Based on the modern science and technology sysuem established by QIAN Xue-sen, related problems about the development of the foundational theory of Chinese medicine is discussed.Methods of multidisciplinary synthesis, such as generation of Chinese medicine and Metasynthesis, are applied to study the rationality of the foundational theory of Chinese medicine.It is emphasized that the study of "qualitative intelligence" is as important as the study of "quantitative intelligence".关键词：modern science and technology system;foundational theory of Chinese medicine;multidisciplinary synthesis;qualitative intelligence
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发布时间：2021-09-15 -
- 摘要：There are lacks of terms for mild cognitive impairment in pro-dementia in ancient medical literatures of traditional Chinese medicine （TCM）. Most of TCM terms for this abnormal state adopt the glossaries of dementia. In order to record the disease progress objectively and establish the fundamental terms for syndrome study and standardization of syndrome differentiation, we explored the standardization of TCM terms for the disease.The study is based on the long-term clinical observation and ancient medical literatures, summarizing the terms of TCM for describing mild cognitive impairment in pro-dementia from the ability of memory, language, executive function, sense, and calculation.关键词：dementia;mild cognitive impairment;terms of traditional Chinese medicine
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发布时间：2021-09-15 -
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- 摘要：objective To discuss the immunological connotation of modulating mechanism of "lung corresponding to autumn",and to provide new ideas for the essence of Zangxiang and experimental basis to the cognition of pathology and physiology mechanism for seasonal attack of respiratory system diseases.Method we adopted pinealectomized rats model to measure rats index of spleen and thymus on vernal equinox and autumnal equinox（ using weighting method）,T lymphocyte transformation rate of spleen（using MTT assay）, alveolar macrophages phagocytosis（using phagocytosis of chicken erythrocytes）, IFN-γ in BALF（using ELISA assay）, IL-10 in the lung tissues（using RIA）.Results ① The indexes of spleen and thymus in the normal group and the sham operated group were lower on autumn equinox than on vernal equinox with significant differences. The indexes of spleen and thymus in the model group showed no significant differences between autumn equinox and vernal equinox.② T lymphocyte transformation rate of spleen in all three of the groups were lower on autumn equinox than on vernal equinox with the remarkable differences （P<0.01）. T lymphocyte transformation rates of spleen in the sham operated group and the model group were obviously higher than in the normal group in the same season （P<0.01）. ③ The alveolar macrophages phagocytosis in the normal group was obviously lower on autumn equinox than on vernal equinox with the significant differences.The differences on vernal equinox and autumn equinox were disappeared in the sham operated group and the model group.④ IFN-γ levels in BALF in the normal group were slightly lower on autumn equinox than on vernal equinox without the significant differences.They were slightly higher on autumnal equinox than on vernal equinox in the sham operated group and the model group without the significant differences. ⑤ IL-10 levels of lung tissues in the normal group were slightly lower on autumn equinox than on vernal equinox without the significant differences.They were slightly higher on autumnal equinox than on vernal equinox in the sham operated group without the significant differences.They were slightly lower on autumn equinox than on vernal equinox in the model group without the significant differences.Conclusions ① The seasonal rhythm of lower in autumn and higher in spring is exist in the immune function in lung and body. It may be one of the pathology and physiology bases for respiratory system diseases frequently-occurring in autumn.② Pineal gland play a certain role in modulating the seasonal rhythm of immunity.③ The essence of "lung corresponding to autumn" is that the modulate ability of immune function in body and lung is lower in autumn.Pineal gland-Melatonin is one of the intermediary material of the lung immunity seasonal rhythm changes.关键词：lung corresponding to autumn;pineal gland;immune function;rat
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发布时间：2021-09-15 - 摘要：Objective To study the effect of Zuogui Pills and Yougui Pills on clinical course and the levels of interleukin-1α （IL-1α）, interleukin-4（IL-4）, interferon-γ （IFN-γ） and tumor necrosis factor-α （TNF-α） in plasma of experimental autoimmune encephalomyelitis （EAE） Lewis rats.Methods Lewis rats were immunized with the myelin basic protein （MBP）. Rats were divided randomly into normal-group （Group A）, EAE-group （Group B）, prednisone-group （Group C）, Zuogui Pills group （Group D） and Yougui pills group （Group E） after immunization. Rats were monitored daily, the weight and the clinical score of the degree of paralysis were recorded. On day 15 and 27 after immunization, rats were killed and the peripheral blood was collected for cytokines examination.Results The incidence of EAE was significantly lower （P<0.05） and the weight was significantly heavier （P<0.05） in Group E than in other groups. in Group E.The paralysis score was significantly lower （P<0.05） in Group D and Group E than in Group B and Group C. On day 15 after immunization, the level of IL-4 was significantly higherin Group D than in Group A and Group C （P<0.05）, and the level of IFN-γ and TNF-α were significantly higher in Group D than in Group A, Group B and Group C （P<0.05）. On day 27 after immunization, the level of IL-1α was significantly higher in Group B than in Group A（P<0.05）, the level of IL-4 and TNF-α was significantly higher in Group E than in Group A and Group B（P<0.05）, and the level of IFN-γ was significantly higher in Group E than in Group A（P<0.05）. The level of IL-1α of Group B was significantly higher on day 15 than on day 27 after immunization （P<0.05）. The level of IL-4, IFN-γ and TNF-α of Group D was significantly higher on day 15 than on day 27 after immunization （P<0.05）.Conclusion Yougui Pills can decrease the incidence of EAE. Zuogui Pills and Yougui Pills both are better than prednisone in alleviating clinical signs.Yougui Pills has the effect on increasing weight of EAE rats.Prednisone, Zuogui Pills and Yougui Pills can down-regulate the level of IL-1α in serum. Zuogui Pills can up-regulate the level of IFN-γ, TNF-α and IL-4 in acute phase.Yougui Pills can up-regulate the level of IFN-γ,TNF-α and IL-4 in paracmasis.
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发布时间：2021-09-15 -
- 摘要：Objective To observe the changes of pain threshold and ENK and β-EP contents in the spinal cord and the hypothalamus, and to study the central analgesic effect of acupotomy dissolution in rats with transverse syndrome of the third lumbar vertebrae.Methods Twenty-eight SD rats were randomly divided into normal control group,model control group, acupotomy dissolution group and electroacupunture（EA） group, with 7 cases in each group. Model was established by operation. Acupotomy dissolution was applied twice, EA was applied six times. Radiation heat stimulation was applied to the rat tail and the pain threshold（PT） was detected by tail flick latency test. The test would be done 9 times. ENK and β-EP contents in spinal cord and the hypothalamus were detected with enzyme linked immunosorbent assay（ELISA） on day 28.Results In the model group, the pain threshold values were lower than in the normal group and ENK and β-EP contents in the spinal cord and the hypothalamus were increased significantly （P<0.01）. In the acupotomy dissolution group and EA group, the pain threshold values were higher than in the model control group, and ENK and β-EP contents in the spinal cord and the hypothalamus were decreased significantly（P<0.01）. ENK and β-EP contents in the acupotomy dissolution group were closer to those in the normal control group than in the EA group（P<0.05）.Conclution Acupotomy dissolution and EA can raise the pain threshold value by adjusting ENK andβ-EP of central nervous system.Acupotomy dissolution was more effective.关键词：acupotomy dissolution;transverse syndrome of the third lumbar vertebrae;pain threshold;electroacupuncture;ENK;β-EP;rat
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发布时间：2021-09-15 -
Effects of salvianolic acid B on rat cortical neurons during combined oxygen and glucose deprivation
摘要：Objective To investigate the effects of salvianolic acid B （SalB） on the primary cultured rat cortical neurons during combined oxygen and glucose deprivation.Methods Primary cultured rat cortical neurons were randomly divided into the control group,the model group and the SalB group.The cell model was established by depriving oxygen and glucose for a period of 4 hours. The cortical neurons viability was determined by MTT assay. The leakage rate of lactate dehydrogenase （LDH） was measured by chromatometry. The mitochondria membrane potential （MMP）, the cytosolic free calcium concentration and the apoptosis rate were quantitatively!analyzed by flow cytometry.Results In the SalB group the cortical neurons vitality （0.450±0.105）, the survival rate （58.20±13.56）% and the fluorescence value of MMP （126.24±12.09） were obviously higher than those, 0.336±0.037, （43.40±4.73） % and 109.75±10.15 in the model group. Obvious difference was observed between the two groups （P0.05～0.01）. In addition, in the SalB group the leakage rate of LDH （15.84±1.47） %, the cytosolic free calcium （66.75±5.93） and the apoptosis rate （4.82±2.48）% were significantly lower than those, （27.39±3.75）%, 101.09±16.08 and （10.83±2.13）% in the model group （P0.01）.Conclusion SalB can protect the primary cultured rat cortical neurons during combined oxygen and glucose deprivation through maintaining the calcium homeostasis, stabilizing the mitochondria membrane potential and reducing the apoptosis rate.关键词：neuron culture;salvianolic acid B;mitochondria membrane potential;calcium over1oad;apoptosis;rat- 8
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发布时间：2021-09-15 - 摘要：Objective To compare the difference of syndrome manifestation in C57BL/6N mice with H22 liver cancer and Lewis lung carcinoma.Methods Mice models with H22 liver cancer and Lewis lung carcinoma were established by inoculating H22 liver cancer ascites or cell suspension of Lewis lung carcinoma in armpit of C57BL/6N mice.C57BL/6N mice inoculated with normal sodium were taken as normal control. The four diagnostic methods workstation was used to collect the information which was adopted to practice quantitative syndrome differentiation.Results From the 11thd to the 21thd, the tumor growth and death speed of Lewis lung carcinoma mice were faster than those of H22 liver cancer mice. The survival rate was 43.75%（7/16）and 25.0 %（5/20）in H22 liver cancer mice and Lewis lung carcinoma mice on the 21th day.The asthenia syndrome developed spontaneously in both H22 liver cancer mice and Lewis lung carcinoma mice. And the asthenia syndrome in Lewis lung carcinoma mice occurred early and seriously, and developed quickly.Conclusion Just like the human being,H22 liver cancer mice and Lewis lung carcinoma mice present spontaneity and development of syndrome manifestation, which can be applied to imitate the development of syndrome manifestation in clinical tumor patients and can be used to study about the treatment based on syndrome differentiation. In addition, the quantitative syndrome differentiation adopted by us is helpful to treatment based on syndrome differentiation individually in tumor mice and to evaluate the curative effect objectively and quantitatively.关键词：liver cancer;lung carcinoma;H22;Lewis;Syndrome manifestation;treatment based on syndrome differentiation;mice
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发布时间：2021-09-15 - 摘要：Objective To establish an implantable animal model of prostate cancer, and to apply it to evaluate the anti-tumor activity of medicine. Methods Transgenic adenocarcinoma mouse prostate cell（TRAMP-C2）suspensions were implanted to C57BL/6N mice subcutaneously at oxter. TRAMP-C2 was went down to the future generates in mice until the biological features were stable. The anti-cancer activity of cyclophosphamide（CTX）, TCM recipe Zilongjin and TCM complex prescription were tested on this mice model. Results The biological features were stable after four generations. The weight of tumor reached 2-3g four weeks after hypodermic inoculation of 1×107cells. The growth inhibiting ratios of tumor were 83.23%, 29.58% and 36.03% for CTX, Zilongjin and TCM complex prescription, respectively. Conclusion The establishment of TRAMP-C2 implantable mice model provided a new model for the study of prostate cancer medicine treatment.关键词：prostate cancer;animal model;transgenic adenocarcinoma mouse prostate cell（TRAMP-C2）;Chinese materia medica;mice
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发布时间：2021-09-15 - 摘要：Objective To investigate the effects of Fufangdanshen on choline acetyl transferase （ChAT） and acetyl cholinesterase （AChE） activities in hippocampus and cerebral cortex, and the spatial discrimination in rats with Altheimer’s disease induced by amyloid beta peptide injection.Methods Aβ1～40 was injected into hippocampus of adult Sprague-Dawley rats, and all tests were performed 14 days later. The deposition of senile plaques and the formation of neurofibrillary tangles were observed by thioflavin-S stain.Morris water maze was used to monitor the spatial discrimination. Colorimetric method was used to detect the activity of ChAT and AchE.Results Neurofibrillary tangles and senile plaques were detected in the hippocampus in the model rats after Aβ injection. In spatial discrimination experiment, the rats showed a remarkable time prolonging in platform searching, with a significant increase in swimming distance after Aβ injection. The ChAT activity in both hippocampus and cerebral cortex decreased significantly after Aβ injection. There was no significant change observed in AChE activity though a little enhancement appeared. Administration of Fufangdanshen 1.05 g/kg and 0.525 g/kg significantly alleviated the impairment in spatial discrimination caused by Aβ;ChAT activity was clearly improved when Fufangdanshen was administrated after Aβ.We did not observe an obvious effect of Fufangdanshen on AchE activity.Conclusion Fufangdanshen could improve the impairment of spatial discrimination, and alleviate the brain ChAT activity decrease in AD rats induced by Aβ.
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发布时间：2021-09-15 - 摘要：Objective To establish the assay method for galgravin、veraguensin content from effective fraction of A.tatarinowii Schott. Method The chromatographic column was kromasil ODS C18（250 mm×4.6 mm,5 μm） with 30 ℃,the mobile phase was methanol to water（70∶30） with a flow velocity of 1.0 mL/min,and the detection wavelength was 230 nm in the reverse phased-high performance liquid chromatography （RP-HPLC） procedure.Result The average recovery of veraguensin was 99.65%（RSD=1.12%）, and the average recovery of galgravin was 99.90%（RSD=1.56%）.Conclusion The procedure, which is easy and accurate, can be used as a quality control method for the effective fraction of A.tatarinowii Schott.关键词：effective fraction of A.tatarinowii Schott.;galgravin;veraguensin;high performance liquid chromatography;content determination
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发布时间：2021-09-15 - 摘要：Objective To induce D.giraldii callus by using plane tissue culture technique and to systematically study the chemical components of the D.giraldii cultured cells.Methods High performance liquid chromatography（HPLC）, Gel column chromatography, liquid-liquid extraction, silica gel thin-layer chromatography were used to isolate the compositions and the spectrum was used to identify the structure of the compositions.Results Five compounds were isolated and identified as follows:daphneolone （Ⅰ）, umbelliferone （Ⅱ）, daphnetin-8-O-β-D-glucoside （Ⅲ）, daphnoretin （Ⅳ） and syringin（Ⅴ）.Conclusion It was the first time using plant cell culture technology to obtain D. giraldii callus and the best inductive condition was established. The C6-C5-C6 carbon skeleton components were isolated from D.giraldii culture cells for the first time.关键词：D.giraldii;culture of callus;secondary metabolites;chemical constituents
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发布时间：2021-09-15 - 摘要：Objective To find different expressed proteins of acute spinal cord injury（SCI） and discuss the function of them to study the mechanism of SCI. To observe the effect of GV electroacupuncture on SCI.Methods Seventy-five rats were divided into five groups randomly: Blank Group, Sham Operated Group, Model Group, Drug Group and EA Group, with 15 rats in each group. The model rats were made a spinal cord contusion by using a modified Allen’s method.. After SCI EA Group was treated by electroacupuncture at "mingmen"（DU4） and "dazhui"（DU14） points; Drug Group was treated by methylprednisolone（MP）. Six hours after SCI, the rats were put to death and 2 cm tissue of spinal cord around the injured site were got out and weighed. The proteins were extracted and quantitated. The different expressed proteins were searched by using two-dimensional gel electrophoresis, analyzed by mass spectrum and searched in the data-base.Results Six different expressed proteins were identified:1.Hypothetical 34.4 kd protein in lef3-iap2 intergenic region;2. Ubiquitin carboxyl-terminal hydrolase isozyme L1;3. Balbiani ring protein 3 precursor; 4. Hypothetical 11.0 kDa protein; 5. Keratin, type I cytoskeletal 16; 6. Vesicle-fusing ATPase.Conclusion Acupuncture has therapeutic effect on SCI. The above different expressed proteins take part in scathe and repair through arosing inflammatory cells infiltration and nerve cells apoptosis.
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发布时间：2021-09-15 - 摘要：Objective To study the clinical research literature about ischemic cerebrovascular diseases treated by various acupuncture techniques, namely "brain arousing and resuscitation inducing technique","needling Baihui（DU20） through Qubin（GB7）","Jiao’s three-needle technique" and"temporal three-needle technique" to analyze their combinative effect. Methods A statistical analysis was made according to the average score of the selected articles, and the combination effect of each technique was analyzed with the χ2 method of Cochran-Mantel-Haenszel.Results The combinative effect analysis showed that there was a significant difference between the experimental group and the control group in the internalized literatures with the acupuncture plan of "brain arousing and resuscitation inducing technique" and "temporal three-needle technique". There were significant differences between the control group and the trial group in the "needling Baihui（DU20） through Qubin（GB7）" therapy. In each of the research report of"Jiao’s three needle technique" statistical analysis was done , and it was found that there was significant difference between the experimental group and control group. Conclusion The four accupuncture techniques are effective for treating ischemic cerebrovascular disease. In these reports, detailed statistical methods adopted were not mentioned, so it is difficult to determine wether these methods are correct or not, which directly affects not only the retrospective study, but also accumulation of the research results. In some articals, there were only a few samples in the studies, which affected the statistical analysis. It is suggested that in future study correct estimation of the sample numbers is needed with correct description and application of the statistical methods.关键词："brain arousing and resuscitation inducing technique";"needling Baihui（DU20） through Qubin（GB7）";"Jiao’s three-needle technique";"temporal three-needle technique";ischemic cerebrovascular diseases;literature study
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发布时间：2021-09-15 - 摘要：Objective To get the messages of hepatic fibrosis TCM type of syndromes and it’s basis in modern clinical literatures.Methods By searching in the Chinese National Knowledge Infrastructure（CNKI） and VIP database,literatures according with the inclusion criteria were collected,in which the data of types of syndrome and their basis were get out and the descriptive statistics was made.Results 176 literatures were found, and 34 articles of them were consistent with the inclusion criteria.There were 54 types of syndrome in 34 articles, and 6 types among them presented frequently,including stagnation of liver-qi with deficiency of the spleen, hepatic and renal yin deficiency, asdthenic splenonephro-yang, obstruction of collaterals by blood stasis, humid heat blocked in middle energizer ,qi-stagnancy and blood stasis. Splenohepatomegalia, wiry pulse, and high serum levels of hepatic fibrosis were owned by all 6 types of syndrome. Other symptoms,tongue demonstration, pulse demonstration, and the serum levels of hepatic fibrosis were different.Conclusion The type of syndrome in liver fibrosis is multiplicity and the changes of index of hepatic fibrosis in serum may be the important basis of syndrome differentiation.关键词：hepatic fibrosis;differentiation of symptoms and signs for classification of syndrome;Syndrome manifestation;pattern of syndrome;serum levels of hepatic fibrosis;literature study
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发布时间：2021-09-15 -
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