最新刊期
卷 0 ， 期 0 ， 2020
- 摘要：The animal model of syndrome is a basic tool used in the study of effects and pharmacology of Chinese compound formulas and preparations.It is the precondition for reflecting the effects of Chinese herbal remedies accurately to copy the animal model with TCM syndrome characteristics.A few of judgement methods for animal model of syndrome combined diseases and syndromes include judgement of micro-manifestations or several physicochemical indexes,speculation of syndromes by formulas,and conjecture according to modeling factors.The disadvantages of these methods were analyzed here,and equivalent correspondence of clinical diagnostic criteria was provided for the determination of syndrome attributives in animal model scientifically and rationally.Three questions were expounded in detail,which were very important in the determination of syndrome attributives in animal model by applied the equivalent correspondence of clinical diagnostic criteria,including macro-equivalent correspondence of macroscopic indexes of clinical syndrome diagnosis in model animals,micro-equivalent correspondence of microscopic indexes of clinical syndrome diagnosis in model animals,and micro-equivalent correspondence and association of macroscopic indexes of syndrome diagnosis in model animals.关键词：combination of disease and syndrome;model;criteria;syndromes
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发布时间：2021-05-20 - 摘要：The combination of micro-morphology and TCM symptomatology and its application in clinic and research is important to the establishment of morphological theory system with TCM characteristics.Syndrome differentiation and treatment is one of unique features of TCM diagnosis,which lays stress on pathogenesis.The pathogenesis of endometriosis（EMT） was investigated here from the angle of micro-syndrome-differentiation.Multiple causes were thought as the inducements of kidney deficiency and blood stasis in women through discussing the conformity of immune disorder in women with EMT and in the pqtients with kidney-deficiency and blood-stasis syndrome.The blood stasis was accumulated along with menstrual cycle and EMT showed a progressive severe trend.EMT takes kidney deficiency as its root and blood stasis as its manifestation, so it is deficiency in origin and excess in superficiality.关键词：micro-syndrome-differentiation;endometriosis;pathogenesis;kidney deficiency and blood stasis
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发布时间：2021-05-20 - 摘要：Formula consists of more than two medicinal ingredients and it is a high level form of the application of Chinese herbal remedies（CHR）.A formula with multiple medicinal ingredients is not a simple congregation of the actions of its ingredients though it is formed on the basis of the capability of one single ingredient.There is a complicated parting and reunion relation between formulas and CHR.The signification,phenomenon, cause and meaning of parting and reunion of formulas and CHR were discussed and analyzed here.The parting and reunion of formulas and CHR was thought as the characteristics of convergence and separation in actions showed by formulas and CHR.The phenomenon of parting and reunion of formulas and CHR existed commonly in formula compatibility and application.The cause might be related to that CHR had multiple properties,different combinations,and the diseases and syndromes related to these combinations had complex pathogenesis.The scientific principle of parting and reunion of formulas is revealing by modern research. The parting and reunion of formulas and CHR contains a scientific question of Chinese medicine,i.e.a closer relation among CHR-formula-syndrome-efficacy.It is important academically for revealing the principle of TCM syndrome differentiation and treatment to recognize deeply the relationship between formulas and CHR.关键词：formulas;Chinese herbal remedies;efficacy;parting and reunion of formulas and Chinese herbal remedies
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发布时间：2021-05-20 - 摘要：Objective To analyze the laws of formula design and administration of Chinese medicinal formulas in the treatment of alcoholic hepatiosis with TCM,and discuss the characteristics of TCM therapies in modern times.Method The clinical literature（46 pieces） on the treatment of hepatiosis with TCM in recent 20 years in China were collected.The classification and frequency of Chinese herbal remedies（CHR）,and the main CHR administrated in the treatment were calculated and analyzed.Result CHR with the actions of activating blood circulation,resolving stasis,excreting water and dampness,clearing heat,and regulating qi had higher administrative frequency.Radix Salviae Miltiorrhizae,Radix Bupleuri and Poria were commonly used in the treatment of alcoholic hepatiosis.Conclusion The essential pathogenesis of alcoholic hepatiosis is the accumulation of qi,blood and phlegm.The therapies include mostly activating-blood and regulating-qi therapy and clearing-heat and dredging-dampness.CHR used commonly are those with the actions of activating blood circulation,resolving stasis,excreting water and dampness,clearing heat,and regulating qi.关键词：alcoholic hepatiosis;medication classification;medicine administration laws
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发布时间：2021-05-20 - 摘要：Objective To observe the changes of glutamate receptors 1（GluR1） and glutamate receptors 2（GluR2） expressions in hippocampus and amygdala in rats of chronic restraint stress,and the influence of Xiaoyao Powder.Method Male SD rats（n=30） were randomly divided into three groups（each n=10）,including the normal control group（group A）,model group（group B） and Xiaoyao Powder group（group C）.Group B and C were given chronic restraint to establish the stress model,and group A was scatter-fed in breeding box.From the first day of modeling,group C was fed daily the suspension of effective components of Xiaoyao Powder one hour before the stress,and group A and B were fed equivalent distilled water.After 21 days rats in all groups were anaesthetized and affused.The rat brain was collected,frozen,sliced up,and then stained by immunohistochemistry method.Result In the model group the expression of GluR1 was increased in regions of CA1 and DG,and expression of GluR2 was decreased in regions of CA1,CA3 and DG.The expressions of GluR1 and GluR2 did not change in BLA region.Xiaoyao Powder could down-regulate the expressions of GluR1 positive cells and immune positive substance,and up-regulate the expressions of GluR2 positive cells and immune positive substance.Conclusion The expressions of GluR1 and GluR2 of amino-3-hydroxy-5-methyl-4-isoxazole propionic acid（AMPA） in hippocampus had different trends in the rat model of liver-stagnation and spleen-deficiency syndrome.CA1 region of hippocampus may be the most sensitive region of synaptic plasticity.The regulation effects of Xiaoyao Powder on the expressions of GluR1 and GluR2 show the characteristics of regional-selective,time-phase and double-directional.关键词：chronic restraint stress;AMPA;GluR1;GluR2;liver-stagnation and spleen-deficiency syndrome;Xiaoyao Powder;rats
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发布时间：2021-05-20 - 摘要：Objective To compare the influences of formulas with actions of soothing liver,invigorating spleen or soothing liver and invigorating spleen on immune system in rat model of liver-stagnation and spleen-deficiency syndrome.Method All rats were randomly divided into the normal control group（group 1）,group of liver-stagnation and spleen-deficiency syndrome（group 2）,Chaihushugan Powder group（group 3）,Sijunzi Decoction group（group 4） and Chaishusijun Decoction group（group 5,each n=12）.The model was established in group 2,3,4 and 5 by chronic restraint-fatigue-diet disorder method for 4 weeks.Group 3,4 and 5 were given orally the relevant formulas（10 mL/g） for 2 weeks at the end of 2nd week,and group 1 and 2 were given equivalent distilled water.On the 29th day all rats were killed and serum interleukin-2（IL-2）, interleukin-6（IL-6） and proliferation rate of T lymphocytes were detected.Result Compared with group 1,serum IL-2,IL-6 and proliferation rate of T lymphocytes decreased significantly in group 2（P<0.05）.Compared with group 2,serum IL-2 increased significantly in group 3,4 and 5（P<0.05）,serum IL-6 increased significantly in group 3 and 5（P<0.05）,and proliferation rate of T lymphocytes increased significantly in group 5（P<0.05）.Conclusion Formulas with actions of soothing liver,invigorating spleen or soothing liver and invigorating spleen can relieve the hypoimmune state of the model of liver-stagnation and spleen-deficiency syndrome,among them Chzishusijun Decoction is the best.Formulas with actions of soothing liver and invigorating spleen is related more closely to liver-stagnation and spleen-deficiency syndrome.关键词：relation between formulas and syndromes;liver-stagnation and spleen-deficiency syndrome;interleukin-2;interleukin-6;proliferation rate of T lymphocytes;Chaihushugan Powder;Sijunzi Decoction;Chaishusijun Decoction;rats
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发布时间：2021-05-20 - 摘要：Objective To study the influence of Jianing Formula on the proliferation of FRTL cells processed with thyroid stimulation antibodies（TSAb） and its inducing effect in apoptosis in Fisher rats.Methods The serum samples from patient with Graves disease was processed with polyethylene glycol 4000 to obtain the crude extract of TSAb,and then the latter was used to act on FRTL cells in Fisher rats.All serum samples were divided into 4 groups,including high-dose Jianing Formula group,mid-dose Jaining Formula group,low-dose Jaining Formula group and Jiakangling Tablets control group.After the collective culture of serum samples with FRTL cells,the proliferation of FTRL cells was detected with methyl thiazolyl tetrazolium test（MTT）,and the expressions of bcl-2 and bax mRNA were examined by using the technique of in situ hybridization.Results The crude extract of TSAb promoted the proliferation of FRTL cells.In all groups the proliferation of FRTL cells was inhibited in a manner of dose-dependent,and which was more significant in high-dose Jianing Formula group（P<0.01）.The positive expression rate of bcl-2 decreased in all Jianing Formula groups,and that of bax mRNA increased in mid-dose and high-dose Jianing Formula groups.Jianing Formula reduced the ratio of bcl-2 to bax mRNA,and the effect was better as the dose was higher.Conclusion Jianing Formula can inhibit the proliferation of FRTL cells processed with TSAb,induce the apoptosis,which may be one of mechanisms of Jianing Formula for curing Graves disease.关键词：FRTL cells;Jianing Formula;proliferation;apoptosis;rats
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发布时间：2021-05-20 - 摘要：Objective To observe the therapeutical effect of self-made Bohe Decoction in rabbit model of weifen syndrome of warm disease,and investigate the foundation and meaning of Chinese herbal remedies（CHR） with the actions of activating blood circulation and removing blood stasis administrated in weifen stage of warm disease.Methods The rabbits（n=40） were randomly divided into 4 groups（each n=10）,among them Yinqiao Powder group and Bohe Decoction group were injected intraperitoneally respectively the decocting solution of Yinqiao Powder or Bohe Decoction 2 hours before endotoxin injection and 5 minutes after endotoxin injection.The symptoms and signs in rabbits of all groups were observed.After 0.5,1 and 1.5 hours the rectal temperature（Tr） in rabbits of all groups were taken respectively.The level of endothelin-1（ET-1） in the blood drawn from rabbit heart was detected.Finally the rabbits were killed and their main organs were observed in general and examined pathologically by microscope.Results In the model group the indexes of ΔT,temperature response index after 1.5 hours（TRI1.5） and ET-1 increased significantly with lung subcongestion.In Yinqiao Powder group the symptoms were relieved and ΔT was different significantly compared with that in the model group,but there was no difference in TRI1.5 and ET-1 between two groups and lung congestion was not relieved.In Bohe Decoction group the symptoms,all indexes,macroscopic and pathological observations were not different to those in the blank group,but different significantly to those in the model group.Conclusion Bohe Decoction can induce body temperature effectively,improve ET-1 index and lung congestion in the treatment of weifen syndrome of warm disease.CHR with the actions of activating blood circulation and removing blood stasis used in weifen syndrome of warm disease can play the role of curing disease before syndrome onset,as well as have curative effect on the pathological changes of micro-blood-stasis.关键词：weifen syndrome;Yinqiao Powder;Bohe Decoction;curing disease before syndrome onset;activating blood circulation and removing blood stasis;rabbits
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发布时间：2021-05-20 - 摘要：Objective To establish a method for quantitative determination of kurarinone and norkurarnone in Radix Sophorae Flavescentis by HPLC.Methods HPLC was performed on a column of Phenomenex Gemini C18（250 mm×4.6 mm,5 μm）,and using acetonitrile-water（50∶50） as the mobile phase.The flow rate was 1.0 mL/min and detection wavelength was 290 nm.Results Kurarinone showed a good linear relationship at range of 0.3186～1.5930 μg（r=0.999 8） and norkurarnone,at range of 0.1128～0.5640 μg（r=0.999 9）.The average recovery（n=6） of kurarinone was 100.56%（RSD=0.93%）,and norkurarnone,96.66%（RSD=0.85%）.Conclusion The determinative results of 3 batches of samples showed that the method is easy and accurate with a higher reproducibility,and it can be used in the quantitative determination of kurarinone and norkurarnone in Radix Sophorae Flavescentis.关键词：Radix Sophorae Flavescentis;kurarinone;norkurarnone;HPLC;quantitative determination
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发布时间：2021-05-20 - 摘要：Objective To study the chemical compositions of volatile oil from Rhizoma Atractylodis Macrocephalae and its anti-tumor effect in mice bearing tumor S180.Methods The volatile oil from Rhizoma Atractylodis Macrocephalae was extracted by steam distillation,which was then divided into two parts according to its polarity,i.e.part of high polarity（zbo-2） and part of low polarity（zbo-1）,by using general silicagel column chromatography.The chemical compositions of the volatile oil were separated and purified at the same time,and its structure was determined by nuclear magnetic resonance（NMR） and X-ray diffraction method.The anti-tumor effects of atractylenolide I,atractylenolide III,zbo-2 and zbo-1 in mice bearing tumor S180 were observed taken tumor growth inhibitory rate as the index.Results There were 3 compounds separated from the volatile oil from Rhizoma Atractylodis Macrocephalae,including atractylenolide I,atractylenolide II and atractylenolide III.Atractylenolide I,atractylenolide III,zbo-2 and zbo-1 in low dose and high dose had a weak anti-tumor effect,but zbo-1 in high dose（200 mg/kg） had a stronger anti-tumor effect（tumor growth inhibitory rate was 27.74%）.Conclusion Atractylenolide II was separated for the first time from the volatile oil from Rhizoma Atractylodis Macrocephalae. The anti-tumor active principle may be in the part of low polarity（zbo-1） of the volatile oil from Rhizoma Atractylodis Macrocephalae.关键词：volatile oil from Rhizoma Atractylodis Macrocephalae;atractylenolide;X-ray diffraction;anti-tumor;mice
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发布时间：2021-05-20 - 摘要：Objective To investigate the influence of Ezhuyou-nocardia rubra-cell wall skeleton（Ezhuyou-N-CWS） Carrier Suppository on the growth of Hela cells of cervical cancer in the patients infected by human papillomavirus（HPV）,and review the curative effect of Ezhuyou-N-CWS Carrier Suppository on HPV infection of lower genital tract and its prevention effect on cervical cancer.Method Methyl thiazolyl tetrazolium（MTT） assay was used to examine the inhibitory effect of Ezhuyou-N-CWS Carrier Suppository on the growth of Hela cells of cervical cancer in the patients infected by HPV.The patients（n=60） with HPV16/18 infection of lower genital tract were chosen and randomly divided into Ezhuyou-N-CWS Carrier Suppository group（group 1） and Ezhuyou-N-CWS Liniment group（group 2,each n=30）.After 3-month treatment HPV was detected in two groups and the curative effects in two groups were analyzed.Result The result of t test used in the analysis of data get by MTT assay in two groups showed that the data of two groups at different time points had statistical significance（P<0.05）.The outcome of clinical test showed that the negative rate was 76.67% in group 1 and 80.00% in group 2 after 3-month treatment（χ2=0.098,P=0.754）,which indicated that the difference was not significant statistically between two groups.Conclusion Ezhuyou-N-CWS Carrier Suppository can inhibit obviously the growth of Hela cells of cervical cancer.The curative effect of Ezhuyou-N-CWS Carrier Suppository is as the same as that of Ezhuyou-N-CWS Liniment on HPV infection of female lower genital tract.Both can prevent effectively the onset of cervical cancer,but Ezhuyou-N-CWS Carrier Suppository is more convenient to use.关键词：nocardia rubra-cell wall skeleton;Ezhuyou;human papillomavirus;carrier suppository;liniment
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发布时间：2021-05-20 - 摘要：An acupoint is a special point,an external part of meridian system,through which qi and blood of zang-fu and meridian circulate and distribute in the body,and by which human body communicates with outside world.An acupoint can reflect the physiopathological changes of zang-fu,meridian,qi and blood.It is a stimulation point accepting excitation and transfering this excitation to meridian and zang-fu for regulating their functions,that is one of key sections of acupuncture efficacy in clinic.The location of an acupoint is related to that it is not only a point on body surface,but also a site of vitality-qi motion.So the crux of needling is to catch and regulate spirit.To understand the internal meaning of an acupoint and use it correctly is very important for acupuncture efficacy.The original meaning and locations of an acupoingt,and relationship between acupoint and vitality-qi and relationship between spirit and needling were expounded here to help to understand the concept and meaning of acupoint,and lay a solid foundation for drawing up the national standard of acupuncture terminology.关键词：acupoint;location;nature of an acupoint
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发布时间：2021-05-20 - 摘要：Objective To observe the effects of acupuncture on expressions of brain apoptosis-related genes,i.e.P53,Bcl-2 and Bax, in rats with brain contusion.Method The rat model of brain contusion was established by using impact method of Feeney freely falling body and then all rats（n=40） were randomly divided into 4 groups,including the shamoperation group,model group,naloxone group and acupuncture group（each n=10）.The acupuncture group and naloxone group were given respectively acupuncture and introperitoneal injection of noloxone once a day for 7 days.The expressions of P53,Bcl-2 and Bax in injured brain were detected by using immunohistochemistry assay.Result In the model group the expressions of P53 and Bax were higher significantly than those in the sham-operation group（P<0.01） and the ratio of Bcl-2 to Bax was reduced.In the acupuncture group and naloxone group the expression of Bcl-2 was higher significantly（P<0.01）,expressions of P53 and Bax were lower significantly （P<0.01） than those in the model group,and the ratio of Bcl-2 to Bax was increased.The expressions of P53,Bcl-2 and Bax were not different respectively between the acupuncture group and naloxone group（P>0.05）.Conclusion Acupuncture can reduce the expressions of P53 and Bax,increase Bcl-2 expression,and raise the ratio of Bcl-2 to Bax in rats with brain contusion,which may be one of the mechanisms of acupuncture for relieving brain apoptosis and preventing secondary brain injury.关键词：brain contusion;acupuncture;apoptosis-related genes;p53;ratio of Bcl-2 to Bax;rats
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发布时间：2021-05-20
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