最新刊期
      卷 45 ， 期 11 ， 2022

        Special Theme: TCM Hematology and Oncology

      • CHEN Xinyi
        Vol. 45, Issue 11, Pages: 1081-1088(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.001
        摘要：The characteristics and advantages of traditional Chinese medicine in improving the quality of life of tumor patients were comprehensively reviewed through the research and analysis of relevant literature, both at home and abroad. The Chinese and foreign literature published from January 1, 2012 to April 23, 2022 were searched in the databases of China National Knowledge Infrastructure and PubMed. The two key issues of the research status of the quality of life of tumor patients and the impact of symptom control on the quality of life of tumor patients were summarized and evaluated. In recent years, improving the quality of life of tumor patients has become a hot topic in the medical field. Important measures to enhance or improve the quality of life of tumor patients gradually tend to traditional Chinese medicine treatment. Active treatment of primary diseases and effective control of related symptoms are the key to improving the quality of life of tumor patients. Traditional Chinese medicine has obvious advantages in preventing and treating tumor-related anemia, depression, fatigue, pain, bone marrow suppression, adverse reactions of digestive tract, and peripheral neurotoxicity, which is of great significance for improving the quality of life of tumor patients. However, there are still main problems of traditional Chinese medicine in improving the quality of life of tumor patients, including: clinical research lacks top-level design and multi-center randomized controlled study data with characteristics of traditional Chinese medicine, and the level of evidence needs to be further improved; the endpoint indicators and efficacy evaluation system of how traditional Chinese medicine improves the quality of life of tumor patients urgently need to be evaluated with localized evaluation standards. In the era of precise disease treatment, the advantages of traditional Chinese medicine in improving the quality of life of tumor patients will be more prominent, and the clinical research and efficacy evaluation system is expected to be improved.  
        关键词：tumor;quality of life;traditional Chinese medicine   
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        发布时间：2022-12-03
      • XU Jing,DONG Shi,DONG Qing,HOU Li
        Vol. 45, Issue 11, Pages: 1089-1094(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.002
        摘要：Cancer-related anemia (CRA) is a common complication in the development and treatment of tumors. As an independent risk factor for the prognosis of tumor patients, the overall incidence of CRA is approximately 30% to 90%, which seriously affects the clinical efficacy, quality of life, and survival prognosis. In China, only 7.16% of tumor patients have received treatment for anemia, and only 1.8% of those with mild anemia have received related treatment. At present, CRA has a high incidence rate, low treatment rate, and a general treatment effect. Timely and effective prevention and treatment of CRA is crucial for tumor patients. Traditional Chinese medicine has unique advantages and characteristics in the prevention and treatment of CRA, including promoting bone marrow hematopoiesis and improving hemoglobin levels, as well as combining prevention and treatment by protecting whole blood cells with multiple targets, significantly relieving systemic symptoms, improving quality of life, and reducing the incidence of related adverse events.  
        关键词：cancer-related anemia;traditional Chinese medicine;prevention and treatment;advantages;characteristics   
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        发布时间：2022-12-03
      • ZHANG Jiahui,YU Mingwei,WANG Xiaomin,MENG Hui,CHEN Xing,DING Tongjing,ZHOU Jie,GAO Chong
        Vol. 45, Issue 11, Pages: 1095-1102(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.003
        摘要：ObjectiveTo observe the clinical effect of traditional Chinese medicine in improving the quality of life of patients with triple-negative breast cancer after radiotherapy and chemotherapy.MethodsFrom March 2016 to December 2017, according to the admission and discharge criteria, 314 patients were collected from nine tertiary hospitals in Beijing, including Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University. The traditional Chinese medicine group (198 cases) received traditional Chinese medicine based on syndrome differentiation, while the observation group (116 cases) did not receive traditional Chinese medicine treatment related to triple-negative breast cancer. All of the patients were followed up every 3-6 months until the 3-year follow-up was completed. The patients’ quality of life was evaluated according to the Functional Assessment of Cancer Therapy-Breast (FACT-B), the Hospital Anxiety and Depression Scale (HADS), the Chinese version of Piper Fatigue Scale, and the Pittsburgh sleep quality index.ResultsThe improvement degree of the total score of FACT-B and the scores of physiological condition, emotional state, functional status and additional concerns in the traditional Chinese medicine group were higher than those in the observation group (P<0.05, P<0.01). The degree of improvement of anxiety and depression scores in HADS in the traditional Chinese medicine group was higher than that of the observation group (P<0.01). The improvement in the total score and sub-dimensions of behavior, emotion, feeling and cognition of the Chinese version of Piper Fatigue Scale in the traditional Chinese medicine group were higher than those of the observation group (P<0.01). There was no significant difference between the two groups in terms of the improvement in the total score and each sub-dimension score of the Pittsburgh sleep quality index.ConclusionChinese medicine treatment can improve the degree of fatigue, relieve anxiety and depression in patients with triple-negative breast cancer, and improve the overall quality of life, with good drug safety.  
        关键词：traditional Chinese medicine;triple-negative breast cancer;fatigue;insomnia;anxiety;depression;quality of life   
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      • LU Enhao,ZHU Lihua,ZHANG Ren,WU Guanzhong,FAN Rui,OUYANG Yiran,CHEN Zhiwei,LI Hegen
        Vol. 45, Issue 11, Pages: 1103-1109(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.004
        摘要：ObjectiveTo observe the clinical efficacy of Jinfukang oral liquid combined with adjuvant chemotherapy in the treatment of early-stage non-small cell lung cancer (NSCLC) after radical resection.MethodsA prospective, randomized, controlled study method was used. A total of 198 patients with early-stage Ib to Ⅱb NSCLC after radical resection with syndrome of deficiency of both qi and yin was included, and the patients were divided into a control group (98 cases) and an experimental group (100 cases) by randomized block design. The control group received four cycles of postoperative standard adjuvant chemotherapy, and the experimental group received adjuvant chemotherapy combined with Jinfukang oral liquid at the same time. The differences in traditional Chinese medicine (TCM) symptom score, physical strength score, and disease-free survival rate were compared between two groups after treatment.ResultsIn terms of TCM symptom score, the symptom scores in cough, expectoration, panting, chest pain, lack of strength, inappetence, dry mouth and throat, spontaneous and night sweating, insomnia, and frequent nocturia were smaller in the experimental group compared with the control group (P<0.05, P<0.01). The physical strength scores of both groups improved after treatment compared with before treatment (P<0.05), and the improvement rate of physical strength score in the experimental group was higher than that of the control group (P<0.05). The disease-free survival rates at 12, 24 and 36 months in the experimental group were 90.9%, 84.4% and 83.1%, respectively, while those of the control group were 88.1%, 82.0% and 77.3%, respectively. The disease-free survival rates of the experimental group were higher than those of the control group, albeit not statistically significant.ConclusionJinfukang oral liquid combined with postoperative adjuvant chemotherapy can reduce TCM symptom scores, improve the related symptoms and physical strength of patients with NSCLC, and to some extent delay disease progression.  
        关键词：Jinfukang;treating cancer by strengthening vital qi;non-small cell lung cancer;disease-free survival;radical resection   
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      • LI Ya,LI Xiao,DONG Qing,CHEN Xinyi,LI Yuanqing,LIU Qun,HOU Li
        Vol. 45, Issue 11, Pages: 1110-1118(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.024
        摘要：ObjectiveTo investigate the inhibitory effect and preliminary mechanism of Modified Liangfu Granule (MLFG) on gastric cancer AGS cells.MethodsGastric cancer AGS cells and normal human gastric epithelial cells GES-1 were cultured in vitro, and treated with different mass concentrations of MLFG (4, 5, 6, 7 and 8 g/L). The CCK-8 method was used to detect the inhibitory effect of MLFG on gastric cancer AGS cell proliferation, the scratch test was used to detect the effect of MLFG on the migration of gastric cancer AGS cells, and the expression level of m6A transmethylase METTL3 gene in AGS cells was observed by real-time PCR method. The survival analysis website (http: //kmplot.com/analysis/) was used to analyze the effect of METTL3 expression on the survival time of patients with gastric cancer. The Illuminal platform was used for sequencing, and GO enrichment analysis and KEGG signaling pathway were used to analyze the role of differentially expressed genes in molecular function, biological process, cell composition and signaling pathway after the intervention of MLFG.ResultsThe result of CCK-8 showed that MLFG could inhibit the proliferation of AGS cells in each mass concentration and each time (P<0.01). Scratch test showed that the migration ability of AGS cells in MLFG group was significantly reduced, and each mass concentration of MLFG could inhibit the migration of AGS cells at 24 h and 48 h (P<0.05, P<0.01). The result of real-time PCR showed that the expression level of METTL3 in AGS cells was significantly higher than that in GES-1 cells (P<0.01). Survival analysis of gastric cancer patients showed that the survival time of patients with low METTL3 expression was longer than that of patients with high METTL3 expression(P<0.05). After MLFG intervention, a total of 1 245 up-regulated genes and 1 156 down-regulated genes were screened, among which the expression level of METTL3 was down-regulated (P<0.05). The differentially expressed genes induced by MLFG were involved in the response of cells to chemokines, the negative regulation of mRNA catabolism and the binding process of enzymes, proteins and nucleotides; while the signal pathways were mainly related to cell apoptosis, autophagy regulation, p53 signaling pathway, cellular cycle and classic tumor-related signaling pathways, such as mTOR, MAPK and TNF.ConclusionMLFG can inhibit the proliferation and migration of gastric cancer cells, and its effect may be related to regulating the level of m6A methyltransferase METTL3.  
        关键词：Modified Liangfu Granule;gastric cancer;METTL3;m6A   
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        发布时间：2022-12-03

        Expert Commentary

      • JIA Chunhua,WANG Yongyan
        Vol. 45, Issue 11, Pages: 1119-1123(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.027
        摘要："Relativity between prescription and syndrome" is a timeless topic, and interpreters of different eras are continually injecting new content into it. With the addition of new members "correspondence between prescription and syndrome" and "relevance of prescription and syndrome" , the "relativity between prescription and syndrome" family is constantly growing. If "relativity between prescription and syndrome" is a method of syndrome differentiation, it is undoubtedly necessary to conduct research around the three basic concepts of "prescription-syndrome" , "relative" , and "relativity between prescription and syndrome" . "Prescription-syndrome" is a method of classifying syndromes according to the indications of prescriptions, that is, to name syndromes according to prescriptions. "Relative" is to compare the main symptoms of the prescription with the symptoms displayed by the patient. "Relativity between prescription and syndrome" is to select the prescription according to the degree of "consistency" of the comparison result. The establishment of prescriptions for treating diseases and syndromes originates from the records of previous physicians and the integration of new experiences. The methods of choosing prescriptions and judging whether "prescription-syndrome" are relative are based on syndrome characteristics and pathogenesis characteristics. "Relativity between prescription and syndrome" is a theory that emphasizes "how to do" , and its advantages and disadvantages are suggested to be evaluated from the perspectives of base and application.  
        关键词：relativity between prescription and syndrome;prescription and syndrome;syndrome;Treatise on Febrile Disease;Essentials from the Golden Cabinet   
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        Theoretical Studies

      • LIU Zhenzhu,QIAN Kehong,LIU Xiuchao,LIU Yuxin,ZHAI Shuangqing
        Vol. 45, Issue 11, Pages: 1130-1134(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.023
        摘要：The theory of "aromatics acting on the spleen" is crucial evidence leading aromatic herbs to regulate the spleen to treat related diseases in clinical practice. This article discusses the definitions, origin and the application of the theory of "aromatics acting on the spleen" . "Aromatics acting on the spleen" originates from "spleen is related to fragrance" in Huangdi Neijing, which is one of the five odors. Odor is a concept as important as flavor, which are different from nose to another, causing heart and lungs dominating odors. Aromatics, including cereals and vegetations, refer to harmonious and glorious qi in general. "Aromatics acting on the spleen" is short for "odors that are fragrance being transported to the spleen" , which originates from the theory of five odors in Huangdi Neijing after the Song and Yuan dynasty, during the development of Zang-Fu viscera syndrome differentiation, the medicinal properties theory, and the meridian tropism system. Essentially, "aromatics acting on the spleen" is to operate spleen qi, highlighting the function that the medicinal "qi" affects Zang-fu viscera qi movement, which can both nurture spleen qi, promote rotating in the middle energizer, and invigorate the spleen to resolve dampness, stimulating qi to eliminate dampness. Meanwhile, if aromatics are overused or misused, they could easily damage the spleen qi. Clarification of the theory’s connotation, origin, and forming and developing process has significance in constructing a modern Chinese medicine theoretical framework, implementing the use of aromatic herbs in the clinic, and treating diseases related to spleen and stomach.  
        关键词：fragrance;spleen;five odors;five flavors;Huangdi Neijing;aromatic herbs   
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      • XIONG Weifeng,HE Juan
        Vol. 45, Issue 11, Pages: 1135-1141(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.007
        摘要：Based on the seven chapters of Yunqi in the Huangdi Neijing, this paper traces the literature on the different patterns of its intra-annual calendar and its formation, development and application, and proposes that the calendar system of the seven chapters of Yunqi in the Huangdi Neijing is a collection of diverse ideas under the influence of multiple ideas. The division of the six periods is based on astronomical observations, the well-established yin-yang calendar, and a unique calendrical system formed by the combined influence of the Book of Changes ( "Da Ming Zhong Shi, Liu Wei Shi Cheng" ) and the Zhou Bi Suan Jing (Qi Heng Liu Jian Chart). The application of the four seasons and the 24 solar terms is a practical need for meteorological and climatic observation, which is a manifestation of the theory of Yunqi towards practical application under the influence of agricultural civilization. The division of the five seasons is influenced by the cultural idea of the five elements and has a strong cultural flavor. Comparing the three models, the six periods have a precise calendar system and rules, with strong theoretical attributes, and occupy a dominant position in the calculation mode of Yunqi theory. The four seasons and the 24 solar terms are used for practical observation, with more practical attributes; while the five seasons mode is in line with the five elements cultural idea, which is a reflection of cultural penetration.  
        关键词：Yunqi theory;Huangdi Neijing;intra-annual calendar;astronomical observations;agrarian civilization;five elements   
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        Chinese Medicinal Pharmacology

      • GENG Qiudong,NIU Susheng,ZHANG Yan,WANG Heming,LI Nan
        Vol. 45, Issue 11, Pages: 1142-1150(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.008
        摘要：ObjectiveTo explore the possible mechanism of arthralgia-flaccidity simultaneous treatment recipe Huashi Dingtong decoction and Guilu Erxian glue on osteoarthritis.MethodsHuashi Dingtong decoction and Guilu Erxian glue medicated serum and blank serum were prepared. A stable in vitro culture system of chondrocytes was established using 4-week-old SD rat knee articular cartilage, and the F2 generation chondrocytes were identified by immunohistochemical staining. After successful identification, four volume fractions of medicated serum (5%, 10%, 15%, and 20%) and blank serum were used to treat chondrocytes (5×103 cells/well). After culturing for 24 h, 48 h, and 72 h, the CCK-8 method was used to detect chondrocyte activity, and the best medicated serum volume fraction was selected to enhance chondrocyte activity for follow-up experiments. The F2 generation chondrocytes were induced to replicate the degenerated chondrocyte model with 10 mg/L lipopolysaccharide, and the cells were divided into the following groups: the blank group, model group, Huashi Dingtong decoction and Guilu Erxian glue medicated serum group (medicated serum group). After 24 h of intervention, the chondrocyte activity of each group was detected using the CCK-8 method . The levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in the supernatant of cell culture medium were detected by ELISA. qRT-PCR method was used to detect the mRNA expression of chondrocyte B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), and cysteine protease-3 (Caspase-3) in each group. Western blotting was used to detect the expression of Bcl-2, Bax and Cleaved Caspase-3 proteins in the chondrocytes of each group.ResultsCompared with the blank group, the activity of chondrocytes in the model group decreased (P<0.01), the levels of IL-1β, IL-6 and TNF-α in the cell supernatant increased (P<0.01), the mRNA expressions of Bax and Caspase-3 and the protein expressions of Bax and Cleaved Caspase-3 were up-regulated (P<0.01), and the mRNA and protein expressions of Bcl-2 were down-regulated (P<0.01). Compared with model group, the chondrocyte activity in the medicated serum group increased(P<0.05), the levels of IL-1β, IL-6, and TNF-α in the cell supernatant decreased (P<0.05), the mRNA expressions of Bax and Caspase-3 and the expressions of Bax and Cleaved Caspase-3 proteins were decreased (P<0.05), and the mRNA and protein expressions of Bcl-2 were up-regulated (P<0.05).ConclusionThe arthralgia-flaccidity simultaneous treatment method can significantly improve the inflammatory microenvironment for chondrocyte survival, enhance chondrocyte activity, improve chondrocyte apoptosis, and then play a protective effect on rat degenerated chondrocytes, and delay the pathogenesis of osteoarthritis.  
        关键词：arthralgia-flaccidity simultaneous treatment method;Huashi Dingtong decoction;Guilu Erxian glue;cartilage cells;osteoarthritis;rats   
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      • YE Yifan,YAN Yue,WANG Lei,CHEN Xiaoying,HE Changhao,ZHANG Xiaomeng,SHI Zhongyu,JIANG Huiqian,ZHUANG Xinli,HU Sumin
        Vol. 45, Issue 11, Pages: 1151-1159(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.022
        摘要：ObjectiveTo investigate blood-testis barrier injury in C57BL/6J mice induced by one-time total-body irradiation (TBI) with 6 Gy 60Co γ ray, and the ability of Yiqi Jiedu decoction to protect against this injury.MethodsEighty male C57BL/6J mice were randomly assigned to the following groups according to body mass: blank group, model group, anduolin group (0.54 g/kg), and Yiqi Jiedu decoction group (16.58 g/kg), with 20 mice per group. After a 10-day pretreatment, the mice (except the blank group) were given one-time TBI with 6 Gy 60Co γ ray, following which, treatment was administered from the 4th day to 11th day after radiation. The mice were sacrificed separately on the 2nd day and 12th day after radiation. The testis and epididymis were weighed, and their pathological changes were examined using HE staining. Additionally, the diameter of the seminiferous tubules and the thickness of the seminiferous epithelium were measured. In addition, the serum inhibin B (INH B) was detected using ELISA, and the expressions of claudin-11 and occludin in the testis were determined using Western blotting.ResultsCompared with the blank group, the weight of the testis and epididymis in the model group, anduolin group, and Yiqi Jiedu decoction group did not change significantly on the 2nd day after the TBI, yet all decreased on the 12th day after TBI (P < 0.01). The testis and epididymis in the model group showed pathological damages on the 2nd day that were aggravated on the 12th day, while the diameter of the seminiferous tubules and thickness of the seminiferous epithelium were decreased compared with those of the blank group at both time points (P<0.01). The pathological damages to the testis and epididymis in the Yiqi Jiedu decoction group were both improved when compared with those of the model group, and the diameter of the seminiferous tubules and thickness of the seminiferous epithelium increased compared with the model group at both time points(P<0.01) . On the 12th day after TBI, the INH B level in the model group was lower than that in the blank group (P < 0.05), while that in the Yiqi Jiedu decoction group was increased compared with the model group (P < 0.01). At both time points, the expressions of claudin-11 and occludin in the model group was decreased compared with that in the blank group (P<0.05, P<0.01), while the expressions of claudin-11 and occludin in the Yiqi Jiedu decoction group increased significantly compared with the model group (P<0.01).ConclusionOne-time TBI with 6 Gy 60Co γ ray induced significant damage to the blood-testis barrier of male C57BL/6J mice, while the Yiqi Jiedu decoction effectively alleviated this damage and promoted the recovery of spermatogenic function with a certain protective effect.  
        关键词：Yiqi Jiedu decoction;ionizing radiation;Sertoli cells;blood-testis barrier;mice   
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        Acupuncture-Moxibustion and Tuina

      • ZHANG Yingqi,LIU Zhifeng,YU Tianyuan,WANG Hourong,JIAO Yi,LIU Di,XU Yajing,GUAN Qian
        Vol. 45, Issue 11, Pages: 1160-1167(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.009
        摘要：ObjectiveTo observe the antipyretic effect of Tuina antipyretic six methods on lipopolysaccharide (LPS)-induced fever in infant rabbits, and to explore the effects on the hypothalamic COX-2/PGE2/EP3 signaling pathway.MethodsUsing the random number table method, 24 New Zealand rabbits(two months old)were randomly divided into the control group, model group and Tuina six methods group, with eight rabbits per group. The fever model was replicated by LPS (0.5 mg/L) injection at the ear margin, and the injection volume was 1 mL/kg in model group and Tuina six methods group. In the Tuina six methods group, the following Tuina six methods of antipyretic intervention were used 1 h after LPS injection: opening Tianmen 200 times in 2 min, pushing Kangong 200 times in 2 min, rubbing Taiyang 200 times in 2 min, rubbing Erhougaogu 24 times and pinching three times in 2 min, clearing the Tianheshui 500 times in 5 min, and pushing the spine 300 times in 5 min. Over the same period, the animals in the model and control groups were smeared with the same volume room temperature water in the same are, and grasped without Tuina intervention. The anal temperature was recorded once every 20 min after LPS injection, and the temperature was continuously measured for 180 min. The body temperature of each group was observed, and the maximum body temperature change value and thermal response index (TRI) were calculated. The expression levels of PGE2 and cAMP in the hypothalamus were detected by ELISA, the protein expression levels of COX-2, EP3, and cAMP were detected using Western blotting, and the mRNA expression levels of COX-2, EP3, and cAMP were detected using RT-PCR.ResultsCompared with the control group, the anal temperature of the model group was increased(P<0.01), and the anal temperature of the model infant rabbits in the Tuina six methods group was decreased after the intervention (P<0.01). The maximum body temperature change value and the TRI in the model group and the Tuina six methods group were higher than those in the control group at 60 min after modeling (P<0.01). Compared with the model group, there was no difference in the maximum body temperature change value and TRI 60 min after modeling in the Tuina six methods group. Moreover, 180 min after modeling, the maximum body temperature change value and TRI were increased in the model group(P<0.01), and those were decreased in the Tuina six methods group compared with those in the model group (P<0.01). The ELISA result showed that compared with the control group, the expression levels of PGE2 and cAMP in the hypothalamus of the model group were increased (P<0.01), while those in the Tuina six methods group were lower than those in the model group (P<0.05, P<0.01). The result of Western blotting showed that compared with the control group, the expression levels of COX-2, EP3, and cAMP in the hypothalamus of the model group were increased (P<0.01), while those in the Tuina six methods group were lower than those in the model group (P<0.05, P<0.01). The RT-PCR result showed that compared with the control group, the mRNA expression levels of COX-2, EP3, and cAMP in the hypothalamus of the model group were increased (P<0.01), and those in the Tuina six methods group were decreased, compared with those in the model group (P<0.01).ConclusionTuina antipyretic six methods shows a good antipyretic effect on LPS-induced fever infant rabbits. Its mechanism is achieved by inhibiting the activation of the hypothalamic COX-2/PGE2/EP3 signaling pathway by pyrogen, reducing the level of positive regulatory medium cAMP at the terminal of the body temperature center.  
        关键词：Tuina antipyretic six methods;Fever;hypothalamus;COX-2/PGE2/EP3 signaling pathway;cAMP;infant rabbits   
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      • FANG Yuen,SHU Mengmeng,YANG Weimin,WEI Xuecong,FAN Lijie,MA Huirong
        Vol. 45, Issue 11, Pages: 1168-1174(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.010
        摘要：ObjectiveTo observe the clinical efficacy of acupuncture and moxibustion combined with kidney-tonifying herbs in the treatment of diminished ovarian reserve (DOR) with kidney-essence deficiency syndrome, and its effect on the oxidative stress of follicular fluid and the mitochondrial activity of ovarian granulosa cells in patients with DOR.MethodsSixty DOR infertility patients with kidney-essence deficiency syndrome who were treated with assisted reproductive technology at Hebei Family Planning Science and Technology Research Institute and Hebei Reproductive and Gynecology Hospital from March 2018 to December 2019 were included as the observation objects. The patients were classified into the control group and observation group by random envelope, with 30 cases per group. The control group was treated with the conventional controlled ovarian hyperstimulation technique, while the observation group was treated with acupuncture and moxibustion combined with kidney-tonifying herbs on the basis of the control group. Acupuncture and moxibustion were performed once a week, with no acupuncture or moxibustion and no medication during menstruation. The patients were subjected to continuous treatment for three menstrual cycles, traditional Chinese medicine(TCM) syndrome scores, number of oocytes retrieved, fertilization rate, cleavage rate, excellent embryo rate, cancellation rate of transplantation, and clinical pregnancy rate were observed and compared between the two groups. On the day of serum human chorionic gonadotropin (hCG) injection, the levels of serum estradiol and progesterone were detected. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA) in the follicular fluid, as well as the mitochondrial activity in ovarian granulosa cells were detected in the laboratory.ResultsThere was no significant change in TCM syndrome scores in the control group before and after treatment. Compared with the control group after treatment, the TCM syndrome scores in the observation group were lower than those in the control group, and the TCM syndrome scores in the observation group after treatment were lower than those before treatment (P<0.05). In the observation group, the excellent embryo rate increased, and the cancellation rate of transplantation decreased compared with those in the control group (P<0.05). There was no significant difference in the number of oocytes retrieved, fertilization rate, cleavage rate, and clinical pregnancy rate in the observation group compared with those in the control group. The levels of serum estradiol and progesterone in the observation group were higher than those in the control group on the day of hCG injection (P<0.01). Compared with the control group, the content of GSH-Px in the follicular fluid in the observation group was increased(P<0.05), the levels of SOD, CAT and MDA were not significantly different, and the mitochondrial activity of ovarian granulosa cells was enhanced (P<0.05).ConclusionAcupuncture and moxibustion combined with kidney-tonifying herbs can improve the symptoms of kidney deficiency in DOR patients with kidney-essence deficiency syndrome, and can also effectively increase the levels of serum estradiol and progesterone on the day of hCG injection, increase the excellent embryo rate, and reduce the cancellation rate of transplantation. The mechanism may be related to the enhancement of follicular fluid GSH-Px activity and the enhancement of mitochondrial activity of ovarian granulosa cells.  
        关键词：acupuncture and moxibustion;kidney-tonifying herbs;diminished ovarian reserve;assisted reproduction;ovarian granulosa cells;oxidative damage;mitochondrial activity   
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        Clinical Studies

      • ZHAO Zhonghui,GUO Rongjuan,GAO Wei,LI Yang,YU Yao,LI Junnan,YANG Lixuan,DUAN Wenzhe
        Vol. 45, Issue 11, Pages: 1175-1181(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.006
        摘要：Depression is a serious psychosomatic disease with a complex etiology and pathogenesis. This article, guided by the theory of "deficient-qi induced stagnation" proposed by Academician Wang Yongyan, provides an in-depth analysis of the pathogenic features of "deficient-qi induced stagnation" in depression, and elaborates on the pathogenic features and evolution of depression with deficient-qi as the origin and stagnation as the symptom, and the vicious cycle hypothesis of "inflammation-mitochondrial damage" . It is also proposed that mitochondria is the key therapeutic target to break the vicious cycle, and the protection of mitochondria is the potential biological essence of the "nourishing deficiency and cultivating energy" treatment of depression, which provides new ideas to study the pathogenesis and prevention method of depression.  
        关键词：depression;deficient-qi induced stagnation;pathogenesis;inflammation;mitochondria   
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        发布时间：2022-12-03
      • HAN Xiaomei,WANG Xu
        Vol. 45, Issue 11, Pages: 1182-1188(2022) DOI: 10.3969/j.issn.1006-2157.2022.11.021
        摘要：ObjectiveTo explore the efficacy of Huanglian Heye formula combined with metformin in the treatment of obese type 2 diabetes (T2DM) with damp-heat retention and its influence on vascular endothelial function and arterial elasticity.MethodsA total of 120 obese T2DM patients with damp-heat retention were included. The patients were divided into a control group (60 cases) and an experimental group (60 cases) by the random number table method; the control group was given metformin (0.5 g/time, three times daily), and the experimental group was given Huanglian Heye formula (two times daily) combined with metformin. Both groups were treated for 12 weeks. The clinical effect was compared between the two groups, and the metabolic indexes, including the traditional Chinese medicine(TCM) syndrome score, fasting plasma glucose (FPG), 2 h postprandial plasma glucose (2 h PG), glycosylated hemoglobin (HbA1c), insulin resistance index (HOMA-IR), body mass index (BMI), waist-to-hip ratio (WHR), vascular endothelial function [reactive hyperemia index (RHI), hip-ankle pulse wave velocity (PWV), and lipoprotein-associated phospholipase A2 (Lp-PLA2)] were compared between the two groups before and after treatment.ResultsThe difference in clinical effect between the two groups was statistically significant (P<0.05), and the total effective rate of the experimental group was 95.00%, which was higher than the 80.00% of the control group. Compared with the patients before treatment, the TCM syndrome score, FPG, 2 h PG, HbA1c, HOMA-IR, BMI, WHR, PWV and Lp-PLA2 in the two groups after treatment were significantly lower(P<0.05, P<0.01), while the RHI level was higher(P<0.05, P<0.01). When comparing the two groups after treatment, the TCM syndrome score, 2 h PG, HbA1c, HOMA-IR, BMI, WHR and Lp-PLA2 level in the experimental group were significantly decreased (P<0.01), the RHI level was increased(P<0.05), and the FPG and PWV were decreased (P<0.05).ConclusionHuanglian Heye formula combined with metformin can effectively improve blood glucose and insulin resistance in obese T2DM patients with damp-heat retention, reduce body mass, and improve vascular endothelial function and arterial elasticity function, with good curative effect.  
        关键词：type 2 diabetes;obese;Huanglian Heye formula;vascular endothelial function;arterial elasticity   
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        发布时间：2022-12-03
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