最新刊期
卷 47 ， 期 2 ， 2024
- 摘要：In recent years, there have been advances in the fundamental research of acupuncture, yet the growing disconnect between this research and clinical practice has become increasingly evident. This paper revolves around the establishment of the discipline of Acupuncture Systems Engineering and explores the paradigm shift in acupuncture research from being science-driven to becoming technology-driven, with the aim of reshaping the innovative development path of acupuncture. Acupuncture Systems Engineering aims to investigate acupuncture techniques and their applications, while also seeking to establish research models and method that align with these pursuits, thereby providing new technological means and pathways for the study of acupuncture effects and achieving clinical translation. Under the guidance of Acupuncture Systems Engineering, acupuncture is no longer merely a traditional medical approach but has evolved into a multidisciplinary field of innovative traditional Chinese medicine, incorporating engineering, biology, information technology, artificial intelligence, and more. Through technology-driven research and application, Acupuncture Systems Engineering brings a revitalized and promising future to the field of acupuncture.关键词：acupuncture;acupuncture systems engineering;scientific paradigm;disciplinary development;acupuncture theory
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发布时间：2024-05-20 - 摘要：The symbiotic relationship between humanities and technology constitutes the two pillars of the modern acupuncture academic system. However, in the process of disciplinary technological development, the humanistic aspect of acupuncture has not received deserved attention. Against the backdrop of interdisciplinary integration in scientific and educational development, acupuncture as a field needs to reexamine its own humanistic attributes. Building upon the fruitful achievements of previous scholars in humanistic research, it is essential to continue and expand the research of acupuncture humanities. This article reviews the current accomplishments in acupuncture humanistic research, highlighting the necessity of such research from three perspectives: the humanistic background of traditional Chinese medicine, the need for and inevitability of acupuncture discipline development, and the significance deficit faced by acupuncture research. The article puts forth four research directions: acupuncture literature and theoretical studies, acupuncture history research, acupuncture sociocultural studies, and acupuncture digital humanities research. Additionally, it proposes three fundamental method for conducting acupuncture humanistic research, namely, reading, interviewing, and practical application. Acupuncture and the humanities are, in fact, two sides of the same coin, both driven by the pursuit of truth.关键词：acupuncture humanities;research method;research content;discipline development
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发布时间：2024-05-20 - 摘要：The initial step taken by the author involves an analysis of the merits and demerits associated with the theoretical paradigm of traditional acupuncture and moxibustion, specifically referred to as the macro and humanistic paradigm. By adopting a historical materialism and dialectical perspective, the author elucidates how the theoretical paradigm of traditional acupuncture and moxibustion is constrained by historical and technological circumstances. The proposition suggests that in response to the evolving landscape of science and technology across temporal and spatial dimensions, there is a need to shift and augment the traditional acupuncture and moxibustion paradigm. Specifically, this entails transitioning towards the micro and natural science paradigm. The subsequent analysis focused on the issues present within the current paradigm of acupuncture and moxibustion clinical research. It was posited that while the initial objective is to validate the clinical efficacy of acupuncture and moxibustion, the ultimate aim is to establish the instructive significance of traditional acupuncture and moxibustion theory. Moreover, the absence of novel theories and principles capable of guiding the modern scientific innovation in acupuncture and moxibustion clinical practice was identified. Finally, this paper presents the issues and initial concepts that warrant consideration in future clinical research on acupuncture and moxibustion within the context of a paradigm shift. Specifically, emphasis should be placed on investigating the theory and principles of acupuncture and moxibustion in light of the contemporary scientific understanding of human tissue structure and function. For instance, within the theoretical paradigm of local diagnosis and treatment, it is imperative to direct our attention towards the distinctive attributes and principles governing acupuncture therapy at various tissue levels on the external surface of the body. Similarly, within the remote and holistic diagnosis and treatment paradigm, it is crucial to thoroughly examine the instructive implications and pertinent characteristic principles associated with the neural network, fiber network, and vascular network in the context of clinical acupuncture and moxibustion.关键词：science of acupuncture and moxibustion;external therapy;theoretical paradigm;research paradigm shift;paradigm of clinical research
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发布时间：2024-05-20 -
Construction and transformation of a contemporary acupuncture and moxibustion education paradigm AI导读
摘要：Education paradigm is a relatively stable action structure of education and training and its practical effect. The construction of a modern acupuncture and moxibustion education paradigm is a complicated historical process, which not only follows the internal logic of the academic development of acupuncture and moxibustion, but is also influenced by the development of modern education paradigm and medical and health services. With the establishment of modern acupuncture and moxibustion discipline, marked by Science of Acupuncture and Moxibustion written by the Teaching and Research Group of Acupuncture and Moxibustion Discipline of Jiangsu University of Traditional Chinese Medicine in 1957, the education and training model of acupuncture and moxibustion specialized talents in modern traditional Chinese medicine higher education is gradually formed, and solidified into college professional education model in the 1980s. Deconstructing the problems existing in the contemporary education paradigm of acupuncture and moxibustion mainly include the contradictions and problems between connotation and structure, formation and development, discipline and specialty, and level type and function. The transformation of the modern acupuncture and moxibustion education paradigm can be promoted by studying industry demands, revising training objective, defining discipline connotation, adjusting teaching content, learning from advanced concepts, and changing teaching modes.关键词：acupuncture and moxibustion;acupuncture and moxibustion education;education paradigm;teacher education;college education;major;course;deconstruction;transformation- 148
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发布时间：2024-05-20
Special Theme: Shifting, Exploration, and Practice of Paradigm of Acupuncture
- 摘要：The concept of vessel in Chinese medicine has undergone the evolution from blood vessels to theoretical models, and in its development, the concepts of blood vessels and theoretical models of vessel coexisted and influenced each other, resultting in confusion about the concept of pulse in modern society. This article is about the phylogenetic study of the concept of pulse. Based on the conceptual framework theory and the rules of logical concept division, this article attempts to construct the conceptual framework of vessel within the basic theoretical system of Chinese medicine based on Huangdi Neijing. By analyzing the reasons for the confusion about the modern concept of vessel, including wrong understanding of its phylogenetic origin, improper division of concepts, and the improper substitution of concepts, this article points out that the construction of a logically self-consistent conceptual framework of vessel is helpful to reveal the nature of relevant concepts, to clarify their relationships, to eliminate confusion, and to guide clinical practice and scientific research, so as to promote the healthy development of academic research in Chinese medicine.关键词：vessel;blood vessels;meridians;Collaterals;phylogenetics;conceptual framework
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发布时间：2024-05-20
Theoretical Studies
- 摘要：ZHU Danxi and ZHANG Congzheng were representative doctors from the Jin-Yuan dynasties. They belonged to the Hejian School, but they had notable differences in the order of reinforcing healthy qi and eliminating pathogenic factors. In the classic medical case " ZHU Danxi′s treatment of dysentery", ZHU Danxi adopted the treatment method of " First treating its deficiency and then treating its excess" for patients with excess pathogen, which was contrary to ZHANG Congzheng′s claim that " Treatment should first eliminate the pathogenic factor". By systematically analyzing the similarities and differences between the two methods of eliminating pathogenic factors, it was found that both used the three method of sweat promotion, emetic therapy, and purgation, and both paid attention to avoiding the harmful effects of eliminating pathogenic factors on qi of spleen and stomach. However, in the order of reinforcement and elimination, ZHU Danxi emphasized that " The pathogenic factor should be carefully eliminated, and healthy qi should be protected", while ZHANG Congzheng thought that " The healthy qi should not be tonified before the pathogen is eliminated". In the order of treating the spleen and the stomach, ZHU Danxi often supplemented the stomach qi and then eliminated pathogenic factors in patients with spleen-stomach weakness, while ZHANG Congzheng thought that the pathogenic factor should be eliminated first and then stomach qi should be restored. Taking classic medical records as an example, this paper discusses the reasons for the different method between the two doctors from the viewpoints of their ideological origin, the succession of teaching from a master to his disciples, social background, and geographical environment, so as to provide a basis for further exploring the method of learning about medical records and the perspective of understanding medical records.关键词：ZHU Danxi′s case of dysentery;ZHANG Congzheng;Zhu Danxi;eliminating pathogenic factors;strengthening vital qi to eliminate pathogenic factors;clinical treatment ideas;medicine in the Jin-Yuan dynasties
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发布时间：2024-05-20 - 摘要：ZHU Danxi and ZHANG Congzheng were representative doctors from the Jin-Yuan dynasties. They belonged to the Hejian School, but they had notable differences in the order of reinforcing healthy qi and eliminating pathogenic factors. In the classic medical case " ZHU Danxi′s treatment of dysentery", ZHU Danxi adopted the treatment method of " First treating its deficiency and then treating its excess" for patients with excess pathogen, which was contrary to ZHANG Congzheng′s claim that " Treatment should first eliminate the pathogenic factor". By systematically analyzing the similarities and differences between the two methods of eliminating pathogenic factors, it was found that both used the three method of sweat promotion, emetic therapy, and purgation, and both paid attention to avoiding the harmful effects of eliminating pathogenic factors on qi of spleen and stomach. However, in the order of reinforcement and elimination, ZHU Danxi emphasized that " The pathogenic factor should be carefully eliminated, and healthy qi should be protected", while ZHANG Congzheng thought that " The healthy qi should not be tonified before the pathogen is eliminated". In the order of treating the spleen and the stomach, ZHU Danxi often supplemented the stomach qi and then eliminated pathogenic factors in patients with spleen-stomach weakness, while ZHANG Congzheng thought that the pathogenic factor should be eliminated first and then stomach qi should be restored. Taking classic medical records as an example, this paper discusses the reasons for the different method between the two doctors from the viewpoints of their ideological origin, the succession of teaching from a master to his disciples, social background, and geographical environment, so as to provide a basis for further exploring the method of learning about medical records and the perspective of understanding medical records.关键词：ZHU Danxi′s case of dysentery;ZHANG Congzheng;Zhu Danxi;eliminating pathogenic factors;strengthening vital qi to eliminate pathogenic factors;clinical treatment ideas;medicine in the Jin-Yuan dynasties
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发布时间：2024-05-20
Literatural Studies
- 摘要：ObjectiveWe aimed to (i) investigate the protective effect of active components of water extract of huangqi (milkvetch root) on high glucose (25 mmol/L) induced injury of human umbilical vein endothelial cells (HUVEC) and (ii) elucidate the underlying mechanism.MethodsMale SD rats were continuously fed with water extract [20 g/(kg·d)] for 7 d, and blood samples were colleeted 1, 2 and 4 h after the last gavage, combined with supernatant, and performed ultra-performance liquid chromatography (UPLC-MS) analysis to screen the active components of the extract in rats. Cells were divided into normal group, model group, different active components groups treated with different concentrations, and the concerted application of active components group. Cell viability was measured by Cell Counting Kit-8 to explore the optimal concentration of the effective components of milkvetch root. Oxidative damage was measured by reactive oxygen species (ROS). The enzyme-linked immunosorbent assay measured the contents of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA); the protein expression level of nuclear factor E2 related factor (Nrf 2) and heme oxygenase 1 (HO-1) by Western blotting.ResultsFormononetin, daidzein, and calycosin are the active components of milkvetch root water extract.Compared with the normal group, the cell viability of the model group was increased (P<0.05). Compared with the model group, cell viability was decreased in each concentration(26, 13, 6.5 μmol/L) of formononetin group (P<0.05), a high dose of daidzein (0.11 μmol/L) could reduce cell viability (P<0.05). Compared with the normal group, the ROS levels in the model group were increased(P<0.05); compared with the model group, the ROS levels in the formononetin high and medium concentrations(26, 13 μmol/L) groups, different concentrations of daidzein (0.11, 0.06, 0.03 μmol/L), the calycosin high concentration group (7.00 μmol/L) were decreased (P<0.05). The combined active components inhibitive rate was higher than the single active components, and highest in the high concentration group. Compared with the normal group, Nrf2 and HO-1 protein expressions were decreased in the model group (P<0.05); compared with the model group, Nrf2 and HO-1 proteins were increased in the group with high concentration of concerted application group (P<0.05). Compared with the normal group, SOD, CAT were decreased (P<0.05), MDA increased (P<0.05) in the model group; compared with the model group, SOD, CAT in the high concentration concerted application group were increased (P<0.05), MDA was significantly decreased (P<0.05).ConclusionThe active components of milkvetch root water extract, formononetin, daidzein, calycosin can protect HUVEC from high glucose-induced injury. The mechanism may be related to the regulation of Nrf2, HO-1, SOD, CAT, and MDA expression and their antioxidant effects.关键词：formononetin;daidzein;calycosin;concerted application;milkvetch root;nuclear factor-erythroid 2-related factor 2/heme oxygenase-1 signaling pathway;human umbilical vein endothelial cells;diabetic retinopathy
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发布时间：2024-05-20 - 摘要：ObjectiveWe aimed to (i) compare the effects of renshen (ginseng) and huangqi (milkvetch root) on different cancer cells and (ii) elucidate the molecular mechanisms underlying the similarities and differences in their medicinal properties.MethodsThe effects of ginseng standard extract (RSE75) and milkvetch root standard extract (HQE75) on the proliferative activity of different cancer cells(Hep G2, H22, H1299, A549, EC109, and AKR) were determined by the CCK-8 assay. The effects of RSE75 and HQE75 on the mRNA expression levels of immune factors IL-6, TNF-α, and TGF-β and the balance between DNA methyltransferases (DNMTs) and TET2 in A549 cells were determined by RT-qPCR. The effects of RSE75 and HQE75 on the clonal formation rate, apoptosis, and the cell cycle of A549 cells were detected by a clonal formation assay, Annexin V-FITC/PI staining, and flow cytometry, respectively. The potential targets and pathways of RSE75 and HQE75 in the treatment of lung cancer were explored by RNA sequencing technology.ResultsCompared with the blank group, the survival rate of cancer cells in the RSE75 group was decreased, but there was no significant change in the HQE75 group. The mRNA expression levels of IL-6, TNF-α, and TGF-β were decreased in the RSE75 and HQE75 groups. RSE75 inhibited the clonal formation of A549 cells in a concentration-dependent manner, while HQE75 had no obvious inhibitory effect. Both RSE75 and HQE75 significantly induced apoptosis in A549 cells and blocked DNA replication in the G1/S phase. At the same concentration, RSE75 has a stronger effect on inducing apoptosis than HQE75. The mRNA expression level of DNMTs in the RSE75 group was decreased, the mRNA expression level of TET2 in the RSE75 group was increased, and the mRNA expression level of both DNMTs and TET2 was decreased in the HQE75 group. The RNA sequencing result showed that RSE75 and HQE75 had common targets, including CXCL8, CXCL1, ICAM1, and CCL2. The common pathways of action mainly included the IL-17 signaling pathway, the NF-κB signaling pathway, and the TNF signaling pathway, and most of them were involved in immune regulation. The main difference is that renshen can inhibit the proliferation of cancer cells through the HIF-1 signaling pathway through VEGFA, EGR1, and other targets, while huangqi can regulate amino acid metabolism and the NOD-like receptor signaling pathway through IL-6, TNF, and other targets.ConclusionStudies on the anti-cancer effects and the underlying molecular mechanisms have shown that renshen and huangqi have obvious immunomodulatory effects, but renshen also had significant inhibitory effects on cancer cell proliferation. Renshen could regulate the DNMTs/TET2 balance, while huangqi could inhibit the expression of DNMTs. This study elucidated the different properties of renshen and huangqi the molecular level and provides not only a scientific basis for clinical application of renshen and huangqi, which are both qi-tonifying medicinals, but also ideas for the study of the molecular mechanisms underlying the effects of other TCMs.关键词：ginseng;milkvetch root;anticancer effect;comparison of medicinal properties;DNA methyltransferase;RNA sequencing;molecular mechanism
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发布时间：2024-05-20 - 摘要：ObjectiveTo study the mechanism of the intervention of total flavonoids from Zhongjiefeng (sarcandra[twig and leaf]) on immune thrombocytopenia (ITP) using metabolomics techniques.MethodsUsing Excel random function, 48 male SD rats were randomly divided into the normal control group, the model group, the total flavonoids from Zhongjiefeng low-, medium-, and high-dose groups, and the prednisolone acetate group, with 8 rats in each group. Except for the normal control group, rats in other groups were injected intraperitoneally with rabbit anti-rat platelet serum (5 mL/kg) on days 1, 3, 4, 6, 7, and 8 to replicate an ITP rat model. Rats in the normal control group were injected intraperitoneally with an equal volume of physiological saline. Drug intervention was given on the modeling day once a day. The total flavonoids from Zhongjiefeng low-, medium-, and high-dose groups were gavaged with 31.5, 63.0, and 94.5 mg/kg of total flavonoids from Zhongjiefeng, respectively. The prednisolone acetate group was injected intraperitoneally with 10.0 mg/kg of prednisolone acetate. On days 0, 3, 6, and 9, the number of peripheral platelets in each group of rats was dynamically detected. On day 10, the spleen mass was measured and the spleen index was calculated. Screening and identification of differential metabolites in the spleen of rats in each group were carried out using ultra-high-performance liquid chromatography coupled with four-stage rod time-of-flight mass spectrometry technology and multivariate statistical analysis, and pathway enrichment analysis was conducted.ResultsCompared with the normal control group, the model group showed a decrease in peripheral blood platelets on days 6 and 9, an increase in spleen mass, and an increase in spleen index(P<0.05). Compared with the model group, the number of peripheral platelets on day 6 and 9 of the total flavonoids from Zhongjiefeng low-, medium-, and high-dose groups were increased, the spleen mass of the total flavonoids from Zhongjiefeng high-dose group was decreased, and the spleen indexes of the total flavonoids from Zhongjiefeng medium- and high-dose groups were decreased (P<0.05). Through metabolomics analysis of the spleen, 24 biomarkers related to ITP were identified. Of these, 21 were reduced in the total flavonoids from Zhongjiefeng high-dose group, which were mainly involved in linoleic acid metabolism, cysteine and methionine metabolism, and glycerol phospholipid metabolism.ConclusionThe total flavonoids from Zhongjiefeng may restore the normal regulatory ability of the immune system by regulating spleen linoleic acid metabolism, cysteine and methionine metabolism, and glycerol phospholipid metabolism, in order to inhibit excessive platelet destruction and exert a therapeutic effect on ITP.关键词：total flavonoids from Zhongjiefeng;immune thrombocytopenia;metabolomics;spleen;immunity;rats
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发布时间：2024-05-20 - 摘要：ObjectiveWe aimed to elucidate the effect of Modified Buyang Huanwu Decoction on adipose-derived stem cells (ADSCs) in the omentum majus by analyzing the hepatocyte growth factor (HGF) and transforming growth factor-β1 (TGF-β1) levels, and explore its influence on the TGF-β1/Smad signaling pathway and epithelial-mesenchymal transition (EMT) of peritoneal mesothelial cells and the extracellular matrix, in order to provide a theoretical basis for the clinical prevention and treatment of postoperative abdominal adhesion (PAA).MethodsADSCs were cultured with different concentrations(0、5、10、20 g/L) of Modified Buyang Huanwu Decoction and their conditioned medium (CM) was obtained, HGF and TGF-β1 levels in the CM were determined by ELISA. The CM of ADSCs cultured with Modified Buyang Huanwu Decoction (TCM-ADSC-CM), CM of control ADSCs (ADSC-CM), and conditioned medium of ADSCs with low expression of HGF (Low-HGF-ADSC-CM) were prepared, and their HGF content was detected by ELISA before and after treatment of peritoneal mesothelial cells. TCM-ADSC-CM, ADSC-CM, and Low-HGF-ADSC-CM were used to treat an EMT model of peritoneal mesothelial cells. Western blotting, immunofluorescence (IF), qPCR, and other methods were used to determine the expression levels of proteins related to the TGF-β1/Smad signaling pathway and EMT and FN in peritoneal mesothelial cells.ResultsCompared with the normal group (with 0 g/L Modified Buyang Huanwu Decoction), after treatment with different concentrations of TCM (5, 10, and 20 g/L), the content of HGF in TCM-ADSC-CM was significantly increased (P<0.01) and the content of TGF-β1 was significantly decreased (P<0.01). Compared with ADSC-CM, before treatment of peritoneal mesenchymal cells, the HGF content in TCM-ADSC-CM was significantly increased (P<0.01), and the HGF content in Low-HGF-ADSC-CM was significantly decreased (P<0.01). Based on the Western blotting, qPCR, and IF results, compared with the model group, CM in each group could reduce the phosphorylation level of Smad3 (P<0.05, P<0.01) and down regulate the expression of SNAIL1 (P<0.05, P<0.01). The effects were ordered as follows: TCM-ADSC-CM > ADSC-CM > Low-HGF-ADSC-CM. Compared with the model group, CM in each group increased the expression of E-cad (P<0.05, P<0.01), decreased the expression of VIM and FN (P<0.05, P<0.01), inhibited EMT in peritoneal mesothelial cells, and reduced FN deposition, and the effects were ordered as follows: TCM-ADSC-CM > ADSC-CM > Low-HGF-ADSC-CM.ConclusionThe low level of HGF in the omentum majus may be an important factor for the occurrence of PAA. Modified Buyang Huanwu Decoction prevents and treats PAA by blocking the TGF-β1/Smad signaling pathway to inhibit EMT of peritoneal mesothelial cells, reducing the deposition of ECM proteins such as FN, and increasing HGF production and decreasing TGF-β1 production by ADSCs in the omentum majus.关键词：Modofied Buyang Huanwu Decoction;postoperative abdominal adhesion;peritoneal mesothelial cells;adipose-derived stem cells;hepatocyte growth factor;epithelial-mesenchymal transition
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发布时间：2024-05-20 - 摘要：ObjectiveTo investigate the mechanism of Qiangxin Decoction treating chronic heart failure(CHF) by regulating miR-125b-5p and thus inhibiting the expression of nuclear factor-κB (NF-κB)-mediated inflammatory factors in order to protect the cardiovascular endothelial progenitor cells.MethodsAccording to the random number table method, 42 SPF-grade SD rats were divided into the control group, the model group, the Qiangxin Decoction low-dose group (7.45 g/kg), the Qiangxin Decoction medium-dose group (14.90 g/kg), the Qiangxin Decoction high-dose group (29.80 g/kg), and the sacubitril valsartan group (10.42 mg/kg), with 7 rats in each group. Except for the control group, the CHF model was established by ligating the left anterior descending branch of the coronary artery in each group. Two days after modeling, the rats were treated with the corresponding drugs by gavage for 28 days. Cardiac function indexes, including left ventricular end-diastolic internal diameter (LVEDd), left ventricular end-systolic internal diameters (LVEDs), and left ventricular ejection fraction (LVEF), were examined by cardiac ultrasound examination, and the pathological changes in the myocardial tissue of the left ventricle of the rats were observed by HE staining. Myocardial fibrosis was observed in each group by Masson staining. Fluorescence in situ hybridization (FISH) was performed to determine the expression of miR-125b-5p in endothelial progenitor cells of the left ventricular myocardial tissue. The protein expressions of brain-derived neurotrophic factor (BDNF), NF-κB p65 and p-NF-κB p65 in the left ventricular myocardial tissue were detected by Western blotting. Enzyme-linked immunosorbent assay was used to detect the expression of inflammatory factor tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6.ResultsCompared with the control group, LVEDd and LVEDs were elevated and LVEF was reduced in the model group; the pathological damage of myocardial tissue was severe with abnormal changes in the tissue structure, striated muscle became fuzzy, and cardiomyocytes were disordered in their arrangement and partially denatured and necrotic; blue collagen fibers and mucus deposition in the myocardial tissues were increased; the average optical density of miR-125b-5p was increased in the left ventricular myocardial tissue; the protein expression of BDNF was decreased, and the ratio of p-NF-κB p65/NF-κB p65 was increased; the contents of TNF-α, IL-1β, and IL-6 were significantly increased (P<0.05). Compared with the model group, LVEDd and LVEDs were reduced, and LVEF was increased in the Qiangxin Decoction medium-, high-dose groups and the sacubitril valsartan group; the pathological damage of myocardial tissues in the left ventricle was improved, with clear striated muscle, neat and orderly arrangement of cardiomyocytes, and reduction of cellular necrosis; blue collagen fibers and mucus deposition were reduced, and myocardial fibrosis was significantly reduced; the average optical density of miR-125b-5p in the left ventricular myocardial tissue was significantly reduced; the protein expression of BDNF was increased, and the ratio of p-NF-κB p65/NF-κB p65 was reduced; the contents of TNF-α, IL-1β, and IL-6 were significantly reduced (P<0.05).ConclusionQiangxin Decoction can reduce miR-125b-5p expression, inhibit the activation of NF-κB pathway, reduce the expression of the pro-inflammatory factors TNF-α, IL-1β, and IL-6, attenuate the myocardial inflammatory response, pathological injury and reduce myocardial fibrosis, ultimately protect the cardiovascular endothelium, and improve cardiac function to treat CHF.关键词：chronic heart failure;Qiangxin Decoction;miR-125b-5p;NF-κB;inflammatory response;cardiovascular endothelial progenitor cell;rats
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发布时间：2024-05-20 - 摘要：ObjectiveExploring the mechanism of Qizhu Kangai Formula in regulating the key protein of protein tyrosine phosphatase 1B (PTP1B) of homo-heterologous adhesion crosstalk inhibition of colon cancer metastasis.MethodsPrepare blank serum and Qizhu Kangai Formula containing serum. Analysis of effective chemical components in serum containing Qizhu Kangai Formula using liquid chromatography-mass spectrometry technology. Constructing PTP1B overexpression model(OE-PTP1B HCT116) in vitro through phasmid transfection. After detecting the cell survival rate of OE-PTP1B HCT116 using CCK-8 method, select 20% medicated serum and 5%、10%、20% Qizhu Kangai Formula containing serum. Scratch assay, transwell assay, and adhesion assay were used to detect the migration, invasion, and adhesion of cells overexpressing PTP1B, respectively. Western blotting was used to detect protein expression level of PTP1B, vinculin, E-cadherin, integrin αν subunit and integrin β3 subunits.ResultsLiquid chromatography-mass spectrometry analysis revealed that the serum of Qizhu Kangai Formula contains 12 main effective chemical components. Among them, the highest content is Calycosin-7-glucoside. The expression levels of PTP1B and vinculin proteins were increased (P<0.05), indicating the successful construction of the model. Compared with the blank serum group, the survival rate of cells overexpressing PTP1B was decreased in different volume fractions of Qizhu Kangai Formula containing serum groups (P<0.05). Compared with the blank serum group, the wound healing rate of cells in the 10% and 20% Qizhu Kangai Formula containing serum groups decreased, the number of invasive cells in each containing serum group decreased, the number of HCT116 cells adhering to cells in each Qizhu Kangai Formula containing serum group increased, the number of HCT116 cells adhering to the matrigel decreased. Compared with the blank serum group, the expression level of E-cadherin protein was increased(P<0.05), the expression level of PTP1B, vinculin, integrin αν subunit, and integrin β3 subunit were reduced in the 20% Qizhu Kangai Formula containing serum group.ConclusionThe mechanism of Qizhu Kangai Formula in inhibiting colon cancer metastasis may be related to the regulation of homo- and hetero-adhesion crosstalk mediated by PTP1B.关键词：Qizhu Kangai Formula;colon cancer;protein tyrosine phosphatase 1B;homologous adhesion;heterogenous adhesion;rats
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发布时间：2024-05-20
Experimental Studies
- 摘要：ObjectiveTo observe the clinical effect of Xinjing Buan Yewo Bushui Formula on female perimenopausal insomnia with heart-kidney disharmony pattern based on Fu Qingzhu′s theory of " heart-mind interdependence" .MethodsThe perimenopausal insomnia patients with heart-kidney disharmony pattern who were treated in the department of obstetrics and gynecology, Affiliated Hospital of Shanxi University of Chinese medicine from April 2021 to June 2022 and met the inclusion criteria were collected and randomly divided into the experimental group (n=39) and the control group (n=39).The experimental group was treated with Xinjing Buan Yewo Bushui Formula, while the control group was treated with Tianwang Buxin Dan for 4 weeks.The Pittsburgh Sleep Quality Index (PSQI), modified Kupperman (KI) score and Chinese medicine syndrome scores were observed; the polysomnography were monitored; the serum levels of estradiol (E2), follicle-stimulating hormone (FSH), Luteinizing Hormone (LH) and gonadotropin-releasing hormone (GnRH) were measured and compared between the two groups before and after treatment.ResultsThe total effective rate of the experimental group was 91.43 %, and the total effective rate of the control group was 88.89%, the difference was not statistically significant. Compared with before treatment, in the experimental group, the PSQI and KI score were decreased after treatment. Among the sleep process parameters, sleep efficiency was increased, sleep latency, arousal times and awake times all were decreased. Among the sleep structure parameters, the sleep time of non-rapid eye movement stage 2 and stage 3 was prolonged and the proportion was increased, while the sleep time of non-rapid eye movement stage 1 and rapid eye movement stage was shortened and the proportion was decreased. The level of E2 was increased while the level of FSH, LH, and GnRH were decreased(P<0.05).ConclusionXinjing Buan Yewo Bushui Formula can obviously improve sleep quality of perimenopausal insomnia patients with heart-kidney disharmony pattern, control perimenopausal symptoms, regulate sex hormone levels, and has no obvious adverse reactions. It can be used as an alternative prescription for the treatment of female perimenopausal insomnia with heart-kidney disharmony pattern, and it can be popularized and applied.关键词：perimenopausal period;heart-kidney disharmony pattern;insomnia;FU Qingzhu;feeling anxious and restless, lying awake at night
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发布时间：2024-05-20 - 摘要：ObjectiveWe aimed to investigate the characteristics of pattern factors of traditional Chinese medicine (TCM) in patients with pulmonary arterial hypertension (PAH) associated with congenital heart disease (CHD) and analyze the relationship between the dominant TCM pattern factors in patients with PAH-CHD and prognostic indicators.MethodsPAH-CHD patients from the Department of Cardiology, Yan'an Affiliated Hospital of Kunming Medical University were enrolled from October 2020 to January 2022, and information on the four diagnostic criteria of TCM and disease-related indicators was collected. According to the mean pulmonary arterial pressure (mPAP), PAH-CHD patients were divided into the mild group (25 mmHg ≤ mPAP ≤ 35 mmHg), the moderate group (35 mmHg<mPAP ≤ 50 mmHg), and the severe group (mPAP > 50 mmHg). Factor analysis and cluster analysis were conducted to study the distribution characteristics of PAH-CHD pattern factors, and difference analysis and multiple linear regression analysis were conducted to investigate the relationship between pattern factors and prognostic indicators [N-terminal pro-B-type natriuretic peptide(NT-proBNP), 6-minute walking distance, tricuspid annular plane systolic excursion (TAPSE)].ResultsA total of 146 PAH-CHD patients were included in the study, including 43 in the mild group, 29 in the moderate group, and 74 in the severe group. The proportions of males, Hb, mPAP, and pulmonary vascular resistance (PVR) were the highest in the severe group, followed by the moderate group and finally the mild group. The pulmonary/systemic circulation blood flow ratio (Qp/Qs) was the highest in the mild group, followed by the moderate group and finally the severe group. The high-frequency pattern factors of PAH-CHD patients in each group were qi deficiency > phlegm turbidity > blood stasis > water fluid. The appearance of water fluid is accompanied by an increase in NT-proBNP, a decrease in 6-minute walking distance, and a decrease in TAPSE. Qi deficiency, water fluid, blood stasis, and phlegm turbidity are the main disease characteristics of PAH-CHD patients.ConclusionQi deficiency, phlegm turbidity, blood stasis, and water fluid are the main types of pattern factors in PAH-CHD patients. Water fluid is closely related to disease prognosis. Understanding the laws of TCM pattern factors in PAH-CHD patients will be helpful for the diagnosis and treatment of PAH-CHD patients.关键词：congenital heart disease;pulmonary arterial hypertension;TCM pattern factors;mean pulmonary arterial pressure;N-terminal pro-B-type natriuretic peptide;6-minute walking distance;tricuspid annular plane systolic excursion
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发布时间：2024-05-20
Clinical Studies
- 摘要：ObjectiveTo evaluate the clinical efficacy and safety of soothing the liver and regulating the mind in the treatment of primary breast cancer complicated with generalized anxiety disorder, and to explore its immune mechanism.MethodsA randomized controlled study was conducted to include patients who met the inclusion criteria in Guang′anmen Hospital, China Academy of Chinese Medical Sciences from August 2022 to February 2023. According to the 1: 1: 1 random principle, patients were divided into three groups: the traditional Chinese medicine group(n=30), the auricular acupuncture group(n=30), and the control group (n=28). The three groups all received Western medicine standard treatment. On the basis of Western medicine standard treatment, the traditional Chinese medicine group received modified prescriptions for soothing the liver and regulating the mind, and the auricular acupuncture group received auricular acupuncture therapy for soothing the liver and regulating the mind. The course of treatment was 8 weeks. The scores of Hamilton Anxiety Scale (HAMA), Self-rating Anxiety Scale (SAS) and World Health Organization Quality of Life-BREF (WHOQOL-BREF), and the contents of interleukin(IL)-1β, IL-8, IL-10, IL-6, IL-2, IL-4, interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) were evaluated before and after treatment among the three groups. The HAMA score reduction rates of the three groups after treatment were calculated, and the total effective rates were compared.ResultsAfter treatment, the HAMA, SAS, and WHOQOL-BREF family friction scores in the traditional Chinese medicine group and the auricular acupuncture group were all lower than those before treatment (P<0.05), and the WHOQOL-BREF physiology dimension, social dimension, environment dimension, and self-assessment scores were higher than those before treatment (P<0.05). There were significant differences in HAMA, SAS, and WHOQOL-BREF physiology dimension, social dimension, environment dimension, self-assessment, and family friction scores among the three groups after treatment (P<0.05). The scores of HAMA, SAS and WHOQOL-BREF family friction in the traditional Chinese medicine group were lower than those in the control group, while the scores of WHOQOL-BREF physiology dimension, social dimension, environment dimension, and self-assessment score in the traditional Chinese medicine group were higher than those in the control group (P<0.05). The scores of HAMA and SAS in the auricular acupuncture group were lower than those in the control group, and the scores of WHOQOL-BREF physiology dimension, social dimension, and environment dimension in the auricular acupuncture group were higher than those in the control group (P<0.05). There was no significant difference between the traditional Chinese medicine group and the auricular acupuncture group in the scale score after treatment. After treatment, the contents of IL-1β, IL-8, IL-10, IL-2, IFN-γ, and TNF-α in the traditional Chinese medicine group were lower than those before treatment (P<0.05), while there was no significant difference in the auricular acupuncture group before and after treatment. The total effective rate of HAMA in the traditional Chinese medicine group was 83.3%, in the auricular acupuncture group it was 76.7%, and in the control group it was 10.7%. There was a statistical difference among the three groups (P<0.05), while there was no statistical difference between the traditional Chinese medicine group and the auricular acupuncture group.ConclusionTraditional Chinese medicine therapy of soothing the liver and regulating the mind, including prescriptions and auricular acupuncture, can effectively relieve the anxiety of breast cancer patients, improve the quality of life, and is safe without side effects, so it has a certain clinical value.关键词：soothing the liver and regulating the mind;breast cancer;anxiety;auricular acupuncture
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发布时间：2024-05-20
Hematology and Oncology
- 摘要：ObjectiveWe aimed to explore the mechanism of action of cervical transverse process tip acupuncture in a rat model of posterior circulation ischemic vertigo (PCIV) based on the G protein-coupled receptor (Mas)/protein kinase A(PKA)/cyclophosphoadenosine effector binding protein (CREB) pathway.MethodsAccording to the random number table method, 32 male SD rats were divided into the sham operation group (n=8), the model group (n=8), the nimodipine group (n=8), and the electroacupuncture group (n=8). In all groups except for the sham operation group, the PCIV model was established by ligating the right common carotid artery (CCA) and the right subclavian artery (SCA). In the sham operation group, only the right CCA and the right SCA were removed and sutured directly without ligation. The nimodipine group was given intragastric administration of nimodipine suspension (12 mg/kg), and the electroacupuncture group selected C2, C4, and C6 transverse process tip for acupuncture, and performed electroacup uncture treatment on both sides of C2 and C6, 20 minutes each time. There was no intervention in the sham operation group and the model group. The intervention was given once a day for 14 days. Cerebral blood flow, the decrease decline rate of cerebral blood flow, and the step-down avoidance time were recorded in each group. Apoptosis of neurons in the vestibular nucleus of the right brainstem was observed by TUNEL staining, and the protein expression levels of Mas, PKA-Ca, CREB, p-CREB, Bcl-2, and Bax in the vestibular nucleus of the right brainstem were determined by Western blotting.ResultsBefore intervention, compared with the sham operation group, cerebral blood flow in the right vestibular nucleus was decreased and decline rate of cerebral blood flow in the right vestibular nucleus was increased in the remaining three groups (P<0.05), which suggested that the model was successfully established. After intervention, compared with the sham operation group, the model group showed lower cerebral blood flow in the right vestibular nucleus, larger decline rate of cerebral blood flow in the right vestibular nucleus, apoptosis rate in the vestibular nucleus of the right brainstem and protein expression level of Bax increased, and reduced protein expression levels of Bcl-2, Mas, PKA-Ca, and p-CREB/CREB (P<0.05). Compared with the model group, the electroacupuncture group and the nimodipine group showed increased cerebral blood flow in the right vestibular nucleus, a smaller decline rate of cerebral blood flow in the right vestibular nucleus, a shorter step-down avoidance time, a decrease in apoptosis rate in the vestibular nucleus of the right brainstem, decreased protein expression level of Bax, and increased protein expression levels of Bcl-2, Mas, PKA-Ca, and p-CREB/CREB (P<0.05). Compared with the nimodipine group, the electroacupuncture group had increased cerebral blood flow in the right vestibular nucleus, a smaller decline rate of cerebral blood flow in the right vestibular nucleus, a shorter step-down avoidance time, a decrease in apoptosis rate in the vestibular nucleus of the right brainstem, decreased protein expression level of Bax, and increased protein expression levels of Bcl-2, Mas, PKA-Ca, and p-CREB/CREB (P<0.05).ConclusionCervical transverse process tip acupuncture can effectively inhibit apoptosis in the vestibular nucleus in rats with PCIV. Its mechanism may be related to improving cerebral blood flow of the vestibular nucleus and activation of the Mas/PKA/CREB signaling pathway, followed by the modulation of the levels of relevant proteins to inhibit apoptosis in the vestibular nucleus.关键词：cervical transverse process tip acupuncture;posterior circulation ischemia;vertigo;cell apoptosis;Mas/protein kinase A/cyclophosphoadenosine effector binding protein pathway;rats
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发布时间：2024-05-20
Acupuncture and Moxibustion
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