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Study on the effect of Huoxue Jiedu Formula on apoptosis of submaxillary gland cells in mice with Sjögren syndrome

  • role： First author第一作者
  • Affiliation：

    Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing 100029, China

  • Introduction：王东峰，男，硕士，主治医师
WANG Dongfeng1,  
  • Affiliation：

    Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China

ZHU Yuelan2,  
  • Affiliation：

    Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China

HOU Xiujuan2,  
  • Affiliation：

    Tongzhou Branch of Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 101121, China

GU Xinyi3,  
  • Affiliation：

    Huguosi Traditional Chinese Medicine Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100035, China

HU Hai4,  
  • role： Corresponding author通信作者
  • Affiliation：

    Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China

  • Email：lifangkai1324@163.com
  • Introduction：LI Fangkai, Master′s Degree, Associate Chief Physician. Dongfang Hospital, Beijing University of Chinese Medicine, No.6, 1st Area, Fangxing Yuan, Fengtai District, Beijing 100078. E-mail: lifangkai1324@163.com
LI Fangkai2*

résumé

ObjectiveTo explore the effects of Huoxue Jiedu Formula on the expression of the apoptosis-related factors B-cell lymphoma-2 (Bcl-2) and survivin in submaxillary gland cells in mice with Sjögren syndrome (SS).MethodsUsing 15 healthy C57BL/6J female mice as the blank control group. Seventy-five of non-obese diabetes mellitus female mice were classified as SS model. They were randomly assigned to the model group, total glucosides of paeony capsule group (TGP group, 0.233 g/kg), Huoxue Jiedu Formula low- (15.925 g/kg), middle- (31.850 g/kg), and high-dose (62.335 g/kg) groups, with 15 mice in each group. The blank control and model groups received distilled water by gavage, and the other groups received respective drug doses by gavage once a day for 8 consecutive weeks. After 1, 2, 4, 6, and 8 weeks of gavage, the salivary secretion volume of the mice was measured. The submaxillary gland index was calculated. Pathological changes in the submaxillary gland of mice were observed by hematoxylin-eosin staining. The expression of apoptosis-related factors Bcl-2 and survivin was detected by Western blotting and real-time fluorescence PCR.ResultsThere was statistical significance in salivary secretion volume from aspects of between-group, time-point, and interaction effects (P<0.05). After 8 weeks of treatment, compared to the blank control group, salivary secretion volume, submaxillary gland index, and protein and mRNA expressions of Bcl-2 and survivin in the model group were decreased, while the pathological score grading increased (P<0.05). Compared to the model group, the TGP group and the Huoxue Jiedu Formula low-, medium-, and high-dose groups showed an increase in saliva secretion after 8 weeks of treatment and an increase in submaxillary gland index, Bcl-2, and survivin mRNA expression levels, and a decrease in pathological score levels. The protein expressions of Bcl-2 and survivin in the Huoxue Jiedu Formula medium- and high-dose groups were increased (P<0.05). Compared to the TGP group, salivary secretion volume and submaxillary gland index in the Huoxue Jiedu Formula low-dose group were lower at weeks 4 and 6, while the pathological score grading was higher (P<0.05). The pathological score grading in the Huoxue Jiedu Formula high-dose group was lower. The protein and mRNA expressions of Bcl-2 in the Huoxue Jiedu Formula low- and medium-dose groups were lower (P<0.05).ConclusionHuoxue Jiedu Formula may relieve the submaxillary gland lesions and increase the salivary secretion volume of the submaxillary gland in SS model mice by regulating the expressions of apoptosis-related factors such as Bcl-2 and survivin in the submaxillary gland.

mots-clés

Huoxue Jiedu Formula;Sjogren syndrome;submaxillary gland;cell apoptosis;mice

References

  1. [1].
    LUPPI F, SEBASTIANI M, SILVA M, et al. Interstitial lung disease in Sjögren′s syndrome: a clinical review[J]. Clin Exp Rheumatol, 2020, 38 Suppl 126(4): 291-300.
  2. [2].
    FOX RI, FOX CM, GOTTENBERG JE, et al. Treatment of Sjögren′s syndrome: current therapy and future directions[J]. Rheumatology (Oxford), 2021, 60(5): 2066-2074.
  3. [3].
    HUANG L, LIU Q, ZHOU TT, et al. Deficiency of β-arrestin2 alleviates apoptosis through GRP78-ATF6-CHOP signaling pathway in primary Sjögren′s syndrome[J]. Int Immunopharmacol, 2021, 101(Pt A): 108281.
  4. [4].
    BENCHABANE S, SLIMANI-KADDOURI A, ACHELI D, et al. Association between increased bcl-2, fas and FasL levels and inflammation extent in labial salivary glands during primary Sjögren′s syndrome[J]. Endocr Metab Immune Disord Drug Targets, 2022, 22(3): 328-338.
  5. [5].
    李方凯，朱跃兰，侯秀娟，等. 活血解毒润燥方对干燥综合征小鼠颌下腺细胞凋亡抑制因子Survivin表达的影响[J]. 中国实验方剂学杂志，2021, 27(24): 84-91.
  6. [6].
    周新尧，姜泉，唐晓颇，等. 干燥综合征中医证候专家共识[J]. 北京中医药大学学报，2023, 46(3): 310-314.
  7. [7].
    梁亦欣，黄小娟，刘小平，等. 基于“燥、毒、瘀”理论探讨中医治疗干燥综合征合并间质性肺疾病[J]. 西部中医药，2023, 36(12): 37-41.
  8. [8].
    国生，崔霞，薛小娜，等. 振腹疗法联合活血解毒方干预原发性干燥综合征的疗效观察[J]. 世界中医药，2020, 15(23): 3675-3679.
  9. [9].
    毛晓娟. 干燥综合征患者外周血中Treg和Th17细胞绝对数量与肠道微生物菌落特征的相关性研究[D]. 太原：山西医科大学，2020.
  10. [10].
    文凤，李清，李萃，等. 东莨菪碱法和高渗盐水法复制小鼠干燥综合征模型的比较[J]. 中国免疫学杂志，2023, 39(2): 261-266.
  11. [11].
    MANFRÈ V, CAFARO G, RICCUCCI I, et al. One year in review 2020: comorbidities, diagnosis and treatment of primary Sjögren′s syndrome[J]. Clin Exp Rheumatol, 2020, 38 Suppl 126(4): 10-22.
  12. [12].
    LI YF, BOOKMAN AAM. Comparison of effect on sicca symptoms of anticentromere antibody-positive Sjögren syndrome and primary Sjögren syndrome alone[J]. J Rheumatol, 2020, 47(6): 876-880.
  13. [13].
    NEGRINI S, EMMI G, GRECO M, et al. Sjögren′s syndrome: a systemic autoimmune disease[J]. Clin Exp Med, 2022, 22(1): 9-25.
  14. [14].
    ZHU H, ZHENG J, ZHOU Y, et al. Knockdown of RSAD2 attenuates B cell hyperactivity in patients with primary Sjögren′s syndrome (pSS) via suppressing NF-κb signaling pathway[J]. Mol Cell Biochem, 2021, 476(5): 2029-2037.
  15. [15].
    HUANG L, CHEN GS, CHEN Y, et al. Inhibition of melanoma by survivin-specific lymphocytes combined with CCL17 and granulocyte-macrophage colony-stimulating factor in a mouse syngeneic model[J]. Anticancer Drugs, 2021, 32(2): 138-147.
  16. [16].
    MAMRIEV D, ABBAS R, KLINGLER FM, et al. A small-molecule ARTS mimetic promotes apoptosis through degradation of both XIAP and Bcl-2[J]. Cell Death Dis, 2020, 11(6): 483.
  17. [17].
    LI N, LI YS, HU JW, et al. A link between mitochondrial dysfunction and the immune microenvironment of salivary glands in primary Sjögren′s syndrome[J]. Front Immunol, 2022, 13: 845209.
  18. [18].
    何加乐，周新尧，李达，等. 基于叶桂“上燥治气，下燥治血”理论探讨干燥综合征的发病与辨治[J]. 中医杂志，2023, 64(23): 2401-2406.
  19. [19].
    邓予新，孙鼎，顾军花，等. 养阴清热活血法治疗干燥综合征的临床舌象研究[J]. 西部中医药，2022, 35(10): 107-111.
  20. [20].
    姜利芳，刘璐，李雪梅. 益气养阴、活血祛风治疗原发性干燥综合征[J]. 吉林中医药，2021, 41(6): 763-766.
  21. [21].
    王皓，刘英，姜萍. 基于“燥毒-玄府”理论辨治干燥综合征撷粹[J]. 江苏中医药，2023, 55(1): 52-54.
  22. [22].
    石岩硕，邱学佳，赵建群，等. 白芍总苷胶囊联合羟氯喹治疗干燥综合征临床疗效的系统评价[J]. 中国医院用药评价与分析，2022, 22(12): 1502-1506.
  23. [23].
    陈爱党，康宁，郭建洲. 白芍总苷胶囊联合来氟米特治疗对类风湿关节炎患者炎性因子、心肌酶谱及不良反应的影响[J]. 实用医院临床杂志，2022, 19(5): 131-134.
  24. [24].
    王海瑜，张玉婷，柏志义，等. 活血解毒方对NOD小鼠肠道菌群的影响[J]. 中医杂志，2022, 63(1): 56-63.
  25. [25].
    闻志轲，王丽莹，高青杰，等. 孟庆良基于“阴虚燥毒”理论治疗干燥综合征[J]. 中医学报，2023, 38(4): 776-780.
  26. [26].
    王庆，李天一，吴国琳，等. 养阴益气活血方对干燥综合征NOD小鼠颌下腺BCL-6和CXCR5表达的影响[J]. 中国免疫学杂志，2020, 36(3): 261-266.
  27. [27].
    霍晓萌. 活血解毒方联合硫酸羟氯喹治疗干燥综合征疗效及对Th17/Treg免疫失衡的影响[J]. 现代中西医结合杂志，2018, 27(12): 1331-1333.
  28. [28].
    张赛，沈正东，郑凌琦，等. 活血解毒方对HSG细胞凋亡中Caspase家族及关键蛋白的影响[J]. 中华中医药杂志，2023, 38(7): 3310-3313.
  29. [29].
    傅天啸，李天一，吴方平，等．基于NF-κB P65/IκBα信号通路探讨养阴益气活血方治疗干燥综合征作用机制[J].中国中西医结合杂志，2022，42(9)：1095-1100.

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