Objective To observe the influences of Ganfujian Formula on the expressions of transforming growth factor-β1（TGF-β1）

TβRII-mRNA

Smad2/3

Smad4 and Smad7 in rats with hepatic fibrosis induced by porcine serum.Methods The hepatic fibrosis model was established in SD rats by the intraperitoneal injection of porcine serum（0.5 mL

twice a week for 8 weeks）.The rats then were given Ganfujian Formula orally in different doses（1.50 g/kg

2.25 g/kg and 3.0 g/kg） and colchicines （0.154 g/kg） respectively until the end of 12th week.The expressions of TGF-β1 and TβRII-mRNA were detected by using RT-PCR and the expressions of Smad2/3

Smad4 and Smad7 were detected by applying Western blotting method.Results In the model group

compared with the normal control group

the expressions of TGF-β1 and TβRII-mRNA

Smad2/3 and Smad4 increased significantly and Smad7 expression decreased significantly.After the treatment with Ganfujian Formula the expressions of TGF-β1 and TβRII-mRNA were reduced in the treatment group compared with the model group. Ganfujian Formula regulated downward the expressions of Smad2/3 and Smad4

and regulated upward Smad7 expression at the same time.Conclusion Ganfujian Formula can inhibit the expressions of TGF-β1 and TβRII-mRNA

regulates downward the expressions of Smad2/3 and Smad4

and regulates upward Smad7 expression.It may be its one of the mechanisms for curing hepatic fibrosis that it has an obvious influence on hepatic TGF-βand Smad signal pathway.