DU Shou-ying, ZHAO Shuai-mei, LU Yang. Quality standard for extracts of Zhizi(Fructus Gardeniae)[J]. Journal of Beijing University of traditional Chinese Medicine, 2009, 32(11): 768-772.
DU Shou-ying, ZHAO Shuai-mei, LU Yang. Quality standard for extracts of Zhizi(Fructus Gardeniae)[J]. Journal of Beijing University of traditional Chinese Medicine, 2009, 32(11): 768-772.DOI:
Objective To study a method for the quality control of extracts from Zhizi(Fructus Gardeniae).Methods The quality control of extracts from Zhizi was carried out through thin-layer chromatography(TLC)
examinations of moisture content
residue of ignition
heavy metals and arsenic salt
and ultraviolet spectrophotometry and HPLC.The main chromatographic peaks of fingerprint were identified.Results The experimental sample and control sample in TLC showed the same colored spots at the same position(negative without interference).The results of all examinations were accorded with the China National Pharmacopoeia.In the extracts from Zhizi the total iridoid glycosides showed a good linear relationship at a range of 5.592 - 35.712μg(r=0.999 9)
the recoveries of the samples in the concentrations of high
middle and low were 98.34%
100.67%and 101.07%respectively
and RSD were 2.60%
1.47%and 1.20%respectively(n=3).Geniposide showed a good linear relationship at a range of 0.228 - 1.140μg(r=0.999 9)
the recoveries of the samples in the concentrations of high
middle and low were 104.04%
100.97%and 95.94%respectively
and RSD were 0.17%
0.58%and 0.84%respectively(n=3).Conclusion TLC and HPLC-MS can be used to identify qualitatively the chemical constituents of extracts from Zhizi
ultraviolet spectrophotometry can be used to control the quantity of the total iridoid glycosides
and HPLC can be used to control the quantity of geniposide.The method is simple