Objective To study the regulation of Kudiezi Injection with the actions of dispelling toxin and dredging collaterals on PKCδ/MARCKS signal pathway in rats with acute cerebral ischemia

and investigate the brain protective mechanism of the therapy of dispelling toxin and dredging collaterals.Methods The rat model of local cerebral ischemia induced by middle cerebral artery occlusion（MCAO） was established by using modified Zea Longa suture.All rats were randomly divided into the control group

model group

Chinese medicine group（with Kudiezi Injection） and Western medicine group（with hydroxyfasudil injection）.The neurological functional deficit score was reviewed in all groups 12 hours after model establishment.The pathology of cerebral tissue was observed by the light microscope after HE staining.The expressions of PKCδ

p-PKCδ

MARCKS and p-MARCKS were detected by using immunohistochemistry and Western blotting

and the expressions of PKCδ and MARCKS-mRNA were detected by using real-time fluorescence quantitative polymerase chain reaction（RT-PCR）.Results Kudiezi Injection decreased significantly the neurological functional deficit score（P<0.01）

alleviated the pathological changes of cerebral ischemia

and reduced the expressions of PKCδ

MARCKS and MARCKS-mRNA（P<0.05

P<0.01） in rats with acute cerebral ischemia.Conclusion The therapy of dispelling toxin and dredging collaterals has a brain protective effect in the treatment of acute cerebral ischemia

and the mechanism may be related to that the therapy can inhibit the activity of PKCδ/MARCKS signal pathway in ischemia brain.