Objective To separate the fractions with anticoagulant activity from the pepsin hydrolytic liquid of Quanxie（Scorpio）

and detect the distribution of molecular weight in the active fractions by applying gel filtration chromatography.Methods The methods of UV-detection and Coomassie brilliant blue G250 were used to detect and combine protein fractions of gel chromatography.The anticoagulant activities of different fractions were detected taken activated partial thromboplastin time（APTT） as index.The distribution of molecular weight of the separated active fractions was determined by using MALDI-TOF-MS.Results There two fractions

separated from the pepsin hydrolytic liquid of Quanxie by gel filtration chromatography

with significant anticoagulant activities.The molecular weight distributed from 850 to 5 300.Conclusion The result of anticoagulant test in vitro indicates that gel filtration chromatography is feasible for purifying and separating the pepsin hydrolytic liquid of Quanxie.The outcomes of MALDI-TOF-MS show that the active fraction is micromolecular peptide.