电针对大鼠脊髓损伤后notch1、ps1表达的实验研究

时素华; 李志刚; 宋金玲; 尹明锡; 宋萌; 郑光华

您当前的位置：

首页 >

文章列表页 >

电针对大鼠脊髓损伤后notch1、ps1表达的实验研究

针灸 | 更新时间：2021-05-20

- 电针对大鼠脊髓损伤后notch1、ps1表达的实验研究
- Influences of electroacupuncture on expressions of notch1 and ps1 after spinal cord injure in rats
- 北京中医药大学学报 2011年34卷第10期页码：714-717
- 作者机构：
  
  1. 北京中医药大学第三附属医院
  2. 北京中医药大学
- 作者简介：
- 基金信息：
  
  教育部博士点基金资助项目（No.200800260002）()
- DOI：
  中图分类号： R245
- 纸质出版：2011-10-01
- 稿件说明：
移动端阅览
[1]时素华,李志刚,宋金玲,尹明锡,宋萌,郑光华.电针对大鼠脊髓损伤后notch1、ps1表达的实验研究[J].北京中医药大学学报,2011,34(10):714-717+720.

SHI Su-hua1, LI Zhi-gang2, SONG Jin-ling2, et al. Influences of electroacupuncture on expressions of notch1 and ps1 after spinal cord injure in rats[J]. Journal of Beijing University of traditional Chinese Medicine, 2011, 34(10): 714-717.
[1]时素华,李志刚,宋金玲,尹明锡,宋萌,郑光华.电针对大鼠脊髓损伤后notch1、ps1表达的实验研究[J].北京中医药大学学报,2011,34(10):714-717+720. DOI：

SHI Su-hua1, LI Zhi-gang2, SONG Jin-ling2, et al. Influences of electroacupuncture on expressions of notch1 and ps1 after spinal cord injure in rats[J]. Journal of Beijing University of traditional Chinese Medicine, 2011, 34(10): 714-717. DOI：

摘要

目的探讨电针治疗对大鼠脊髓损伤（SCI）后的保护机理。方法大鼠随机分为模型组、空白组、药物组、电针组

用改良Allen’s打击法制成脊髓损伤模型

分别于损伤后6 h、1 d、7 d取损伤局部脊髓组织

提取总RNA

经RT-PCR反应

测定损伤后脊髓组织内信号通路基因（notch1）、早老素1（ps1）mRNA的表达。结果脊髓损伤后

电针组和药物组均能显著降低脊髓损伤6 h、1 d、7 d后脊髓组织中notch1、ps1 mRNA的表达。与模型组相比较

药物组和电针组notch1、ps1 mRNA表达均降低

并都有显著性差异（P<0.05）;药物组与电针组相比较

电针组notch1、ps1 mRNA表达略高于药物组

但无显著性差（P>0.05）。结论 notch信号通路

在细胞的生长、发育、分化及凋亡中起着十分重要的作用

notch信号的激活可加速并不可逆地促进神经干细胞（NSC）向胶质细胞分化

电针治疗对大鼠脊髓损伤后notch1、ps1表达具有明显的抑制作用。

Abstract

Objective To discuss the protective mechanism of electroacupuncture in rats after spinal cord injure（SCI）.Methods All rats were randomly divided into the model group

blank group

medicinal group and electroacupuncture group.The model of SCI was established by using the modified Allen’s method.The injured spinal tissue was taken after 6 hours

1 day and 7 days respectively for extracting total RNA.The expressions of notch1 and ps1 mRNA were detected by using reverse transcription polymerase chain reaction（RT-PCR）.Results The expressions of notch1 and ps1 mRNA all decreased significantly in the electroacupuncture group and medicinal group after SCI for 6 hours

1 day and 7 days.Compared with the model group

the expressions of notch1 and ps1 mRNA decreased in the medicinal group and electroacupuncture group（P<0.05）.The comparison between the medicinal group and electroacupuncture group showed that the expressions of notch1 and ps1 mRNA were higher a little in the electroacupuncture group but without significant difference（P>0.05）.Conclusion Notch signal passway plays a very important role in the growth

development

differentiation and apoptosis of cells.The activation of notch signal can irreversibly accelerate NSC differentiation to gliacytes.Electroacupuncture has a significant inhibitory effect on the expressions of notch1 and ps1 after SCI.

关键词

Keywords

references

查看原文

浏览量

315

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

电针对高脂血症模型大鼠肝细胞脂噬的影响

电针对腰竖脊肌损伤模型大鼠穴区外泌体释放及肌肉再生修复的影响

电针介导PINK1/Parkin信号通路治疗功能性消化不良模型大鼠的机制研究

电针“足三里”“太冲”对功能性消化不良模型大鼠胃肠道功能及脑－肠轴的影响

基于谷胱甘肽抗氧化系统探讨督脉电针对脑卒中后肢体痉挛大鼠氧化应激的影响