Objective To investigate the influence of Yijing Formula（with the actions of tonifying kidney and replenishing essence

and tonifying and activating blood） on sperm apoptosis in mouse model of oligoasthenospermia（OAT） induced by cyclophosphamide（CTX）.Methods Male Kunming mice（n=40） were randomly divided into the control group（CG）

model group（MG）

small-dose treatment group（SG） and large-dose treatment group（LG）.CG was given intraperitoneal injection of normal saline solution（60 mg/kg） once a day for 5 days

and the MG

SG and LG were given the intraperitoneal injection of CTX（60 mg/kg） once a day for 5 days.On the 6th day SG and LG were given orally Yijing Formula according to the body weight（double or 5 times of human common dosage respectively） once a day for 34 days

and MG was given isopyknic normal saline solution once a day for 34 days.CG was fed normally.On the 40th day all mice were given routine analysis of seminal fluid.The sperm apoptosis rate was detected by using flow cytometer after FITC-AV/PI staining.Results In CG

MG

SG and LG

the sperm count was

respectively

5.20±1.34

1.73±0.03

2.08±0.01 and 3.31±0.56（×109 L-1）;sperm motility was

respectively

（14.49±0.30）%

（6.64±1.88）%

（11.99±1.01）% and（19.40±3.13）%;and motility rate was

respectively

（68.39±15.13）%

（39.96±4.89）%

（62.28±4.43）% and（73.61±5.05）%.The sperm count

sperm motility and motility rate were significantly lower in MG than those in CG（P<0.05）

and those in LG were significantly increased after the intervention of Yijing Formula.There was a significant difference between LG and MG（P<0.05）

but no difference in the sperm motility and motility rate between LG and CG.The sperm apoptosis rate in four groups was

respectively

（8.38±0.45）%

（14.84±1.22）%

（13.25±1.83）% and（8.35±2.1）%.The sperm apoptosis rate was significantly higher in MG than that in CG（P<0.05）.After the intervention of Yijing Formula the sperm apoptosis rate was significantly lower in LG than that in MG（P<0.05）

but no significant difference compared with CG.Conclusion The intraperitoneal injection of CTX can increase sperm apoptosis and decrease sperm count

motility and motility rate in mice.The intervention of large-dose Yijing Formula can decrease sperm apoptosis rate and increase sperm count

motility and motility rate aiming at cure OAT.