参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠组织NF-κB p65蛋白表达及相关炎性因子的影响

李姿慧; 王键; 蔡荣林; 孙娟; 叶铭钢

doi:10.3969/j.issn.1006-2157.2015.05.006

您当前的位置：

首页 >

文章列表页 >

参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠组织NF-κB p65蛋白表达及相关炎性因子的影响

博士之光 | 更新时间：2021-05-20

- 参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠组织NF-κB p65蛋白表达及相关炎性因子的影响
- Effects of Shenling Baizhu San on the protein expression of NF-κB p65and the serum level of related inflammatory cytokines in the colon tissue of rats with ulcerative colitis due to dampness retention and spleen deficiency
- 北京中医药大学学报 2015年38卷第5期页码：315-317
- 作者机构：
  
  1. 安徽中医药大学中医临床学院
  2. 安徽中医药大学针灸经络研究所
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（No.81173172,81302901）();安徽省自然科学基金项目（No.1308085QH155）()
- DOI：10.3969/j.issn.1006-2157.2015.05.006
  中图分类号： R285.5
- 纸质出版：2015-05-01
- 稿件说明：
移动端阅览
[1]李姿慧,王键,蔡荣林,孙娟,叶铭钢.参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠组织NF-κB p65蛋白表达及相关炎性因子的影响[J].北京中医药大学学报,2015,38(05):315-317+360-361.

LI Zi-hui, WANG Jian, CAI Rong-lin, et al. Effects of Shenling Baizhu San on the protein expression of NF-κB p65and the serum level of related inflammatory cytokines in the colon tissue of rats with ulcerative colitis due to dampness retention and spleen deficiency[J]. Journal of Beijing University of traditional Chinese Medicine, 2015, 38(5): 315-317.
[1]李姿慧,王键,蔡荣林,孙娟,叶铭钢.参苓白术散对脾虚湿困型溃疡性结肠炎大鼠结肠组织NF-κB p65蛋白表达及相关炎性因子的影响[J].北京中医药大学学报,2015,38(05):315-317+360-361. DOI： 10.3969/j.issn.1006-2157.2015.05.006.

LI Zi-hui, WANG Jian, CAI Rong-lin, et al. Effects of Shenling Baizhu San on the protein expression of NF-κB p65and the serum level of related inflammatory cytokines in the colon tissue of rats with ulcerative colitis due to dampness retention and spleen deficiency[J]. Journal of Beijing University of traditional Chinese Medicine, 2015, 38(5): 315-317. DOI： 10.3969/j.issn.1006-2157.2015.05.006.

摘要

目的探讨参苓白术散对脾虚湿困型溃疡性结肠炎（UC）大鼠结肠组织NF-κB p65蛋白表达及相关炎性因子的影响。方法将48只Wistar大鼠按照随机数字表分为正常组、模型组、柳氮磺吡啶组、参苓白术散组

每组12只

脾虚湿困型溃疡性结肠炎大鼠模型采用三硝基苯磺酸（trinitrobenzene sulfonic acid

TNBS）/乙醇灌肠结合环境与饮食干预法复制

运用免疫组织化学法检测并比较各组大鼠结肠组织NF-κB p65蛋白表达

ELISA法测定血清IL-6、IL-8、TNF-α水平的变化。结果模型组大鼠NF-κB p65蛋白表达及IL-6、IL-8、TNF-α水平明显高于正常组（P<0.05）

参苓白术散组和柳氮磺吡啶组较模型组显著降低

差异有统计学意义（P<0.05）

参苓白术散组与柳氮磺吡啶组比较差异无统计学意义（P>0.05）。结论参苓白术散可以明显改善UC大鼠结肠组织中NF-κB p65蛋白表达及血清IL-6、IL-8、TNF-α水平

促进肠黏膜的修复。

Abstract

Objective To investigate the effects of Shenlin Baizhu San（ Ginseng

Poria and Atractylodes Macrocephalae Powder） on the protein expression of NF-κB p65 in the colon tissue of rats with ulcerative colitis due to dampness retention and spleen deficiency

as well as the related inflammatory cytokines.Methods 48 Wistar rats were divided into four groups: normal group

model group

sulfasalazine group

and Shenlin Baizhu San group

with 12 rats in each group. The model rats were induced by environment and diet intervention combined with composite trinitrobenzene sulfonic acid and ethanol in enema. The protein expression of NF-κB p65 in the colon tissue of rats were detected with immunohistochemistry methods. Levels of IL-6

IL-8

and TNF-α were measured with ELISA. Results The protein expression of NF-κB p65 and the serum level of IL-6

IL-8

and TNF-α in the model rats were significantly higher than those in normal group（ P < 0. 05）. The protein expression of NF-κB p65 and the serum level of IL-6

IL-8

and TNF-α in Shenlin Baizhu San and sulfasalazine groups significantly decreased

and the difference was significant compared with model group（ P < 0. 05）. The difference between Shenlin Baizhu San and sulfasalazine groups showed no statistical significance（ P > 0. 05）.Conclusion Shenlin Baizhu San could significantly improve the protein expression of NF-κB p65 and the serum level of IL-6

IL-8

and TNF-α in the colon tissue of rats with ulcerative colitis

which could promote the repair of the intestinal mucosa.

关键词

Keywords

references

查看原文

浏览量

871

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

益气活血法对舒张性心力衰竭大鼠心肌细胞钙稳态的影响

二陈汤对多囊卵巢综合征大鼠卵巢组织Bcl-2及Bax蛋白表达的影响

基于离体外翻肠囊法研究滇白珠抗炎镇痛活性部位的肠吸收特性

六味地黄汤对5/6肾切除大鼠肾脏缺氧诱导因子-1α及Twist和E-cadherin表达的影响

大黄颗粒对慢性肾脏病大鼠肠道微生态的影响