Objective To investigate the effects of Pixu Yihao Fang（PXYHF

No.1 Formula for Spleen Deficiency）on the expression of Glucose Transporter（GLUT1）protein（GLUT1）and mRNA in small intestine tissue of FD spleen-deficient rats and functional dyspepsia（FD）the intervention of Pixu I Fang.Methods 70 10-day-old male SD rat pups were randomly divided into normal control group（n=10）

FD model group（single model

n=10）

and spleen-deficient FD model group（double model

n=50）.The normal control group received intragastric administration of 0.2 mL of 2%sucrose

while the single and double model groups were given 0.2 mL of 0.1%iodoacetamide（IA）IA in 2%sucrose

for 6days.6 weeks after birth consecutive days

the Modified multiple platform method（MMPM）was also applied to establish double model for 14 days after week 6.These spleen-deficient FD rats were randomly divided into double model control group

domperidone group

PXYHF low-dose

medium-dose and highdose dose groups

and were orally gavaged with 10 mL/（kg·d）of distilled water

3.125 mg/（kg·d）of domperidone

1.275

2.55

5.1 g/（kg·d）of PXYHF respectively for 14 days.Immunohistochemistry

Western-blot and RT-PCR methods were used to measure the GLUT1 protein expression in liver tissues.Results Compared with the normal group

average optical density of GLUT1 protein in small intestinal mucosal tissue

the expression of GLUT1 protein and GLUT1 mRNA in small intestinal tissue of double model group were decreased（P<0.05

P<0.01）;Compared with double model group

average optical density of GLUT1 protein in small intestinal mucosa tissue

expression of GLUT1 protein and GLUT1 mRNA in small intestinal tissue of low-dose PXYHF were increased（P<0.01）.ConclusionThe expression of GLUT1 gene and protein decreased in the FD rats with spleen deficiency

and Pixu Yihao Fang could increase the expression of GLUT1 gene and protein.