探讨活血养阴法调控Bcl-2/Bax通路改善精索静脉曲张大鼠生精功能的作用机制

梁仪春; 王文; 梁树麟; 付瑞杰; 覃湛; 耿立果; 何超拔; 袁少英

doi:10.3969/j.issn.1006-2157.2024.11.001

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探讨活血养阴法调控Bcl-2/Bax通路改善精索静脉曲张大鼠生精功能的作用机制

“中医药防治男性疾病”专题 | 更新时间：2024-12-02

- 探讨活血养阴法调控Bcl-2/Bax通路改善精索静脉曲张大鼠生精功能的作用机制
- Exploring the mechanism of regulating Bcl-2/Bax pathway by the therapy of promoting blood circulation and nourishing yin to improve spermatogenesis in rats with varicocele
- 北京中医药大学学报 2024年47卷第11期页码：1481-1489
- 作者机构：
  
  1.广州中医药大学第二临床医学院　广州　510006
  2.广东省中医院珠海医院
- 作者简介：
  
  梁仪春，女，在读硕士生
  #袁少英，男，硕士，主任医师，教授，博士生导师，主要研究方向：中西医结合治疗男科病，E-mail：ysy017@126.com
- 基金信息：
  
  广东省中医药局中医药科研项目面上项目(202205111913416600);珠海市科技局项目(2220004000307;2320004000300)
- DOI：10.3969/j.issn.1006-2157.2024.11.001
  中图分类号： R285.5
- 收稿日期：2024-05-23，
  
  网络出版日期：2024-10-10，
  
  纸质出版日期：2024-11-30
- 稿件说明：
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梁仪春, 王文, 梁树麟, 等. 探讨活血养阴法调控Bcl-2/Bax通路改善精索静脉曲张大鼠生精功能的作用机制[J]. 北京中医药大学学报, 2024,47(11):1481-1489.

LIANG Yichun, WANG Wen, LIANG Shulin, et al. Exploring the mechanism of regulating Bcl-2/Bax pathway by the therapy of promoting blood circulation and nourishing yin to improve spermatogenesis in rats with varicocele[J]. Journal of beijing university of traditional chinese medicine, 2024, 47(11): 1481-1489.
梁仪春, 王文, 梁树麟, 等. 探讨活血养阴法调控Bcl-2/Bax通路改善精索静脉曲张大鼠生精功能的作用机制[J]. 北京中医药大学学报, 2024,47(11):1481-1489. DOI： 10.3969/j.issn.1006-2157.2024.11.001.

LIANG Yichun, WANG Wen, LIANG Shulin, et al. Exploring the mechanism of regulating Bcl-2/Bax pathway by the therapy of promoting blood circulation and nourishing yin to improve spermatogenesis in rats with varicocele[J]. Journal of beijing university of traditional chinese medicine, 2024, 47(11): 1481-1489. DOI： 10.3969/j.issn.1006-2157.2024.11.001.

摘要

目的

基于B淋巴细胞瘤－2(Bcl-2)/Bcl-2相关X蛋白(Bax)通路探讨活血养阴法改善精索静脉曲张大鼠生精功能的作用机制。

方法

选取8周龄SD雄性大鼠30只，按随机数字表法分为假手术组、模型组、左卡尼汀组(1.0 g/kg)、活血养阴法低剂量组(5.2 g/kg)、活血养阴法中剂量组(10.4 g/kg)及活血养阴法高剂量组(20.8 g/kg)，每组5只。除假手术组外，其余大鼠均通过经典的Turner法复制精索静脉曲张模型。术后4周，假手术组及模型组大鼠灌胃生理盐水，各给药组灌胃相应药物，每日1次，连续4周。灌胃结束后，使用计算机辅助精子分析仪检测精子质量，HE染色法观察睾丸组织病理学形态并通过Johnsen评分评价睾丸生精功能，TUNEL法检测睾丸细胞凋亡情况，实时荧光PCR法检测睾丸组织Bcl-2及Bax mRNA表达，免疫组织化学法检测细胞色素C蛋白表达。

结果

与假手术组比较，模型组大鼠的精子总数、精子活动率及(a＋b)级精子率均降低(

0.05)；睾丸组织中生精小管排列紊乱，有明显空泡样改变，Johnsen评分下降(

0.05)；睾丸组织细胞凋亡率增加(

0.05)；睾丸组织Bax mRNA表达上升(

0.05)；细胞色素C蛋白阳性表达增加(

0.05)。与模型组比较，活血养阴法中剂量组大鼠的精子总数，低、中剂量组精子活动率，以及低剂量组(a＋b)级精子率均上升(

0.05)；大鼠睾丸病理学结构有不同程度的改善，活血养阴法中剂量组Johnsen评分增加(

0.05)；左卡尼汀组与活血养阴法各剂量组睾丸细胞凋亡率均下降(

0.05)；活血养阴法低、高剂量组Bcl-2 mRNA表达上升(

0.05)，左卡尼汀组与活血养阴法中剂量组Bax mRNA表达下降(

0.05)；活血养阴法中剂量组细胞色素C蛋白阳性表达下降(

0.05)。

结论

活血养阴法可提高精索静脉曲张大鼠的精子质量，修复损伤的睾丸组织结构，改善生精功能，其作用机制可能与激活Bcl-2/Bax凋亡通路、抑制细胞凋亡有关。

Abstract

Objective

To explore the mechanism of the therapy of promoting blood circulation and nourishing yin(PBCNY) in improving spermatogenesis in rats with varicocele based on Bcl-2/Bax pathway.

Methods

Thirty 8-week-old male SD rats were randomly divided into six groups (five rats per group): sham

model

levocarnitine (1.0 g/kg)

and PBCNY low-dose (5.2 g/kg)

PBCNY medium-dose (10.4 g/kg)

and PBCNY high-dose (20.8 g/kg) groups. Apart from the sham group

all the groups were built a varicocele model by the classical Turner method. After 4 weeks

the sham group and model group were gavaged with normal saline

and the remaining groups were given with levocarnitine or different doses of PBCNY decoction once a day for 4 weeks. At the end of gavage

the sperm quality of rats in each group was detected by a computer-assisted sperm analyzer

and HE staining was used to observe the testicular tissue morphology of rats in each group and performed with Johnsen score. TUNEL method was used to detect apoptosis

RT-qPCR was used to detect Bcl-2 and Bax mRNA expression

and immunohistochemical method was used to detect cytochrome C protein expression.

Results

Compared to the sham group

sperm total count

motility rate and percentage of sperm with grade (a+ b) motility were lower in the model group of rats (

0.05). HE staining showed disturbed arrangement of seminiferous tubules in the testicular tissue with significant changes and decreased Johnsen score (

0.05). The result of TUNEL assay showed that the apoptosis rate was increased in the model group (

0.05). Bax mRNA expression was increased in testicular tissue (

0.05). The result of immunohistochemistry showed that the positive expression of cytochrome C was increased in the model group (

0.05). Compared with the model group

the rat sperm total count in the PBCNY medium-dose group

motility rate in the PBCNY low- and medium-dose groups

and percentage of sperm with grade (a+ b) motility in the PBCNY low-dose group increased(

0.05); the pathological structure of rat testis had different degrees of improvement

and Johnsen score increased in the PBCNY me

dium-dose group (

0.05); the apoptosis rate of testicular cells decreased in the levocarnitine group and all doses of PBCNY groups (

0.05); the expression of Bcl-2 mRNA increased in the PBCNY low-dose and high-dose groups (

0.05)

and the expression of Bax mRNA decreased in the levocarnitine group and the PBCNY medium-dose group (

0.05); the positive expression of cytochrome C decreased in the PBCNY medium-dose group (

0.05).

Conclusion

The therapy of PBCNY can improve sperm quality

repair damaged testicular tissue structure and improve spermatogenic function in rats with varicocele

and its mechanism of action may be related to the activation of Bcl-2/Bax pathway and inhibition of cell apoptosis.

关键词

Keywords

references

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